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Protein

DNA mismatch repair protein MSH3

Gene

MSH3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. MSH3 provides substrate-binding and substrate specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with either the MutL alpha or MutL beta heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. MutS beta also has a role in regulation of heteroduplex formation during mitotic and meiotic recombination. MutS beta binds to DNA flap structures predicted to form during recombination, and is required for 3' non-homologous tail removal (NHTR). MutS beta-binding alters the DNA conformation of its substrate at the ds/ssDNA junction and may facilitate its recognition and/or cleavage by the downstream nucleotide excision repair (NER) RAD1-RAD10 endonuclease. ATP binding and hydrolysis play a pivotal role in MMR and NHTR.10 Publications

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi791 – 798ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • damaged DNA binding Source: GO_Central
  • DNA-dependent ATPase activity Source: GO_Central
  • DNA insertion or deletion binding Source: SGD
  • double-strand/single-strand DNA junction binding Source: SGD

GO - Biological processi

  • DNA recombination Source: SGD
  • maintenance of DNA repeat elements Source: GO_Central
  • meiotic mismatch repair Source: SGD
  • mismatch repair Source: SGD
  • mitotic recombination Source: SGD
  • removal of nonhomologous ends Source: SGD
  • replication fork arrest Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29386-MONOMER
ReactomeiR-SCE-5358606 Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta)

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MSH3
Alternative name(s):
Mismatch-binding protein
Short name:
MBP
MutS protein homolog 3
Gene namesi
Name:MSH3
Ordered Locus Names:YCR092C
ORF Names:YCR1152, YCR92C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR092C
SGDiS000000688 MSH3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi4Q → A: Partially functional in a mismatch repair assay; when associated with 10-AA-11. 1 Publication1
Mutagenesisi10 – 11FF → AA: Partially functional in a mismatch repair assay; when associated with A-4. 1 Publication2
Mutagenesisi158K → A: Alters DNA-binding activity and impairs MSH2-MSH3-mediated DNA mismatch repair; when associated with ALA-160. 1 Publication1
Mutagenesisi160K → A: Alters DNA-binding activity and impairs MSH2-MSH3-mediated DNA mismatch repair; when associated with ALA-158. 1 Publication1
Mutagenesisi203P → A: No effect. 1 Publication1
Mutagenesisi226Q → A: No effect. 1 Publication1
Mutagenesisi247R → A: Impairs MSH2-MSH3-mediated DNA mismatch repair. 1 Publication1
Mutagenesisi796G → D: Defective in MMR and in NHTR. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001151951 – 1018DNA mismatch repair protein MSH3Add BLAST1018

Proteomic databases

MaxQBiP25336
PaxDbiP25336
PRIDEiP25336

Interactioni

Subunit structurei

Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with either MutL alpha (MLH1-PMS1) or MutL beta (MLH1-MLH3). MutS beta interacts with proliferating cell nuclear antigen (PCNA/POL30). Interacts with SAW1.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31064, 88 interactors
ComplexPortaliCPX-1036 DNA mismatch repair MutSbeta complex
DIPiDIP-2422N
ELMiP25336
IntActiP25336, 39 interactors
MINTiP25336
STRINGi4932.YCR092C

Structurei

3D structure databases

ProteinModelPortaliP25336
SMRiP25336
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 256Mispair-binding domainAdd BLAST131

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi4 – 11PIP box8

Domaini

The PIP box serves as a PCNA(POL30)-recognition and -binding motif.

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074949
HOGENOMiHOG000057130
InParanoidiP25336
KOiK08736
OMAiYHLKSEF
OrthoDBiEOG092C0EWT

Family and domain databases

Gene3Di3.30.420.110, 1 hit
3.40.1170.10, 1 hit
InterProiView protein in InterPro
IPR007695 DNA_mismatch_repair_MutS-lik_N
IPR017261 DNA_mismatch_repair_MutS/MSH
IPR000432 DNA_mismatch_repair_MutS_C
IPR007861 DNA_mismatch_repair_MutS_clamp
IPR007696 DNA_mismatch_repair_MutS_core
IPR016151 DNA_mismatch_repair_MutS_N
IPR036187 DNA_mismatch_repair_MutS_sf
IPR007860 DNA_mmatch_repair_MutS_con_dom
IPR036678 MutS_con_dom_sf
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01624 MutS_I, 1 hit
PF05188 MutS_II, 1 hit
PF05192 MutS_III, 1 hit
PF05190 MutS_IV, 1 hit
PF00488 MutS_V, 1 hit
PIRSFiPIRSF037677 DNA_mis_repair_Msh6, 1 hit
SMARTiView protein in SMART
SM00534 MUTSac, 1 hit
SM00533 MUTSd, 1 hit
SUPFAMiSSF48334 SSF48334, 1 hit
SSF52540 SSF52540, 1 hit
SSF55271 SSF55271, 1 hit
PROSITEiView protein in PROSITE
PS00486 DNA_MISMATCH_REPAIR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P25336-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGQPTISRF FKKAVKSELT HKQEQEVAVG NGAGSESICL DTDEEDNLSS
60 70 80 90 100
VASTTVTNDS FPLKGSVSSK NSKNSEKTSG TSTTFNDIDF AKKLDRIMKR
110 120 130 140 150
RSDENVEAED DEEEGEEDFV KKKARKSPTA KLTPLDKQVK DLKMHHRDKV
160 170 180 190 200
LVIRVGYKYK CFAEDAVTVS RILHIKLVPG KLTIDESNPQ DCNHRQFAYC
210 220 230 240 250
SFPDVRLNVH LERLVHHNLK VAVVEQAETS AIKKHDPGAS KSSVFERKIS
260 270 280 290 300
NVFTKATFGV NSTFVLRGKR ILGDTNSIWA LSRDVHQGKV AKYSLISVNL
310 320 330 340 350
NNGEVVYDEF EEPNLADEKL QIRIKYLQPI EVLVNTDDLP LHVAKFFKDI
360 370 380 390 400
SCPLIHKQEY DLEDHVVQAI KVMNEKIQLS PSLIRLVSKL YSHMVEYNNE
410 420 430 440 450
QVMLIPSIYS PFASKIHMLL DPNSLQSLDI FTHDGGKGSL FWLLDHTRTS
460 470 480 490 500
FGLRMLREWI LKPLVDVHQI EERLDAIECI TSEINNSIFF ESLNQMLNHT
510 520 530 540 550
PDLLRTLNRI MYGTTSRKEV YFYLKQITSF VDHFKMHQSY LSEHFKSSDG
560 570 580 590 600
RIGKQSPLLF RLFSELNELL STTQLPHFLT MINVSAVMEK NSDKQVMDFF
610 620 630 640 650
NLNNYDCSEG IIKIQRESES VRSQLKEELA EIRKYLKRPY LNFRDEVDYL
660 670 680 690 700
IEVKNSQIKD LPDDWIKVNN TKMVSRFTTP RTQKLTQKLE YYKDLLIRES
710 720 730 740 750
ELQYKEFLNK ITAEYTELRK ITLNLAQYDC ILSLAATSCN VNYVRPTFVN
760 770 780 790 800
GQQAIIAKNA RNPIIESLDV HYVPNDIMMS PENGKINIIT GPNMGGKSSY
810 820 830 840 850
IRQVALLTIM AQIGSFVPAE EIRLSIFENV LTRIGAHDDI INGDSTFKVE
860 870 880 890 900
MLDILHILKN CNKRSLLLLD EVGRGTGTHD GIAISYALIK YFSELSDCPL
910 920 930 940 950
ILFTTHFPML GEIKSPLIRN YHMDYVEEQK TGEDWMSVIF LYKLKKGLTY
960 970 980 990 1000
NSYGMNVAKL ARLDKDIINR AFSISEELRK ESINEDALKL FSSLKRILKS
1010
DNITATDKLA KLLSLDIH
Length:1,018
Mass (Da):116,534
Last modified:June 10, 2008 - v2
Checksum:i1AD91C2E2F2856EF
GO

Sequence cautioni

The sequence AAA34803 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA42247 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA46116 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64954 Genomic DNA Translation: CAA46116.1 Different initiation.
M96250 Genomic DNA Translation: AAA34803.1 Different initiation.
X59720 Genomic DNA Translation: CAA42247.1 Different initiation.
BK006937 Genomic DNA Translation: DAA07561.1
PIRiS19508
RefSeqiNP_010016.2, NM_001178798.1

Genome annotation databases

EnsemblFungiiCAA42247; CAA42247; CAA42247
YCR092C; YCR092C; YCR092C
GeneIDi850454
KEGGisce:YCR092C

Similar proteinsi

Entry informationi

Entry nameiMSH3_YEAST
AccessioniPrimary (citable) accession number: P25336
Secondary accession number(s): D6VR92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 10, 2008
Last modified: July 18, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

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