UniProtKB - P25336 (MSH3_YEAST)
DNA mismatch repair protein MSH3
MSH3
Functioni
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. MSH3 provides substrate-binding and substrate specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with either the MutL alpha or MutL beta heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. MutS beta also has a role in regulation of heteroduplex formation during mitotic and meiotic recombination. MutS beta binds to DNA flap structures predicted to form during recombination, and is required for 3' non-homologous tail removal (NHTR). MutS beta-binding alters the DNA conformation of its substrate at the ds/ssDNA junction and may facilitate its recognition and/or cleavage by the downstream nucleotide excision repair (NER) RAD1-RAD10 endonuclease. ATP binding and hydrolysis play a pivotal role in MMR and NHTR.
10 PublicationsMiscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 791 – 798 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA insertion or deletion binding Source: SGD
- double-strand/single-strand DNA junction binding Source: SGD
- double-stranded DNA binding Source: GO_Central
GO - Biological processi
- DNA recombination Source: SGD
- meiotic mismatch repair Source: SGD
- mismatch repair Source: ComplexPortal
- mitotic recombination Source: SGD
- removal of nonhomologous ends Source: SGD
- replication fork arrest Source: SGD
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-5358606, Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) |
Names & Taxonomyi
Protein namesi | Recommended name: DNA mismatch repair protein MSH3Alternative name(s): Mismatch-binding protein Short name: MBP MutS protein homolog 3 |
Gene namesi | Name:MSH3 Ordered Locus Names:YCR092C ORF Names:YCR1152, YCR92C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000688, MSH3 |
VEuPathDBi | FungiDB:YCR092C |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- MutSbeta complex Source: SGD
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 4 | Q → A: Partially functional in a mismatch repair assay; when associated with 10-AA-11. 1 Publication | 1 | |
Mutagenesisi | 10 – 11 | FF → AA: Partially functional in a mismatch repair assay; when associated with A-4. 1 Publication | 2 | |
Mutagenesisi | 158 | K → A: Alters DNA-binding activity and impairs MSH2-MSH3-mediated DNA mismatch repair; when associated with ALA-160. 1 Publication | 1 | |
Mutagenesisi | 160 | K → A: Alters DNA-binding activity and impairs MSH2-MSH3-mediated DNA mismatch repair; when associated with ALA-158. 1 Publication | 1 | |
Mutagenesisi | 203 | P → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 226 | Q → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 247 | R → A: Impairs MSH2-MSH3-mediated DNA mismatch repair. 1 Publication | 1 | |
Mutagenesisi | 796 | G → D: Defective in MMR and in NHTR. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000115195 | 1 – 1018 | DNA mismatch repair protein MSH3Add BLAST | 1018 |
Proteomic databases
MaxQBi | P25336 |
PaxDbi | P25336 |
PRIDEi | P25336 |
Interactioni
Subunit structurei
Heterodimer consisting of MSH2-MSH3 (MutS beta).
Forms a ternary complex with either MutL alpha (MLH1-PMS1) or MutL beta (MLH1-MLH3). MutS beta interacts with proliferating cell nuclear antigen (PCNA/POL30).
Interacts with SAW1.
4 PublicationsBinary interactionsi
P25336
With | #Exp. | IntAct |
---|---|---|
MSH2 [P25847] | 3 | EBI-11362,EBI-11352 |
Protein-protein interaction databases
BioGRIDi | 31064, 97 interactors |
ComplexPortali | CPX-1036, DNA mismatch repair MutSbeta complex |
DIPi | DIP-2422N |
ELMi | P25336 |
IntActi | P25336, 36 interactors |
MINTi | P25336 |
STRINGi | 4932.YCR092C |
Miscellaneous databases
RNActi | P25336, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 21 – 82 | DisorderedSequence analysisAdd BLAST | 62 | |
Regioni | 102 – 127 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 126 – 256 | Mispair-binding domainAdd BLAST | 131 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 4 – 11 | PIP box | 8 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 44 – 82 | Polar residuesSequence analysisAdd BLAST | 39 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0218, Eukaryota |
GeneTreei | ENSGT00550000074949 |
HOGENOMi | CLU_002472_3_1_1 |
InParanoidi | P25336 |
OMAi | LRNVHMK |
Family and domain databases
Gene3Di | 3.30.420.110, 1 hit 3.40.1170.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR007695, DNA_mismatch_repair_MutS-lik_N IPR017261, DNA_mismatch_repair_MutS/MSH IPR000432, DNA_mismatch_repair_MutS_C IPR007861, DNA_mismatch_repair_MutS_clamp IPR007696, DNA_mismatch_repair_MutS_core IPR016151, DNA_mismatch_repair_MutS_N IPR036187, DNA_mismatch_repair_MutS_sf IPR007860, DNA_mmatch_repair_MutS_con_dom IPR036678, MutS_con_dom_sf IPR045076, MutS_family IPR027417, P-loop_NTPase |
PANTHERi | PTHR11361, PTHR11361, 1 hit |
Pfami | View protein in Pfam PF01624, MutS_I, 1 hit PF05188, MutS_II, 1 hit PF05192, MutS_III, 1 hit PF05190, MutS_IV, 1 hit PF00488, MutS_V, 1 hit |
PIRSFi | PIRSF037677, DNA_mis_repair_Msh6, 1 hit |
SMARTi | View protein in SMART SM00534, MUTSac, 1 hit SM00533, MUTSd, 1 hit |
SUPFAMi | SSF48334, SSF48334, 1 hit SSF52540, SSF52540, 1 hit SSF55271, SSF55271, 1 hit |
PROSITEi | View protein in PROSITE PS00486, DNA_MISMATCH_REPAIR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAGQPTISRF FKKAVKSELT HKQEQEVAVG NGAGSESICL DTDEEDNLSS
60 70 80 90 100
VASTTVTNDS FPLKGSVSSK NSKNSEKTSG TSTTFNDIDF AKKLDRIMKR
110 120 130 140 150
RSDENVEAED DEEEGEEDFV KKKARKSPTA KLTPLDKQVK DLKMHHRDKV
160 170 180 190 200
LVIRVGYKYK CFAEDAVTVS RILHIKLVPG KLTIDESNPQ DCNHRQFAYC
210 220 230 240 250
SFPDVRLNVH LERLVHHNLK VAVVEQAETS AIKKHDPGAS KSSVFERKIS
260 270 280 290 300
NVFTKATFGV NSTFVLRGKR ILGDTNSIWA LSRDVHQGKV AKYSLISVNL
310 320 330 340 350
NNGEVVYDEF EEPNLADEKL QIRIKYLQPI EVLVNTDDLP LHVAKFFKDI
360 370 380 390 400
SCPLIHKQEY DLEDHVVQAI KVMNEKIQLS PSLIRLVSKL YSHMVEYNNE
410 420 430 440 450
QVMLIPSIYS PFASKIHMLL DPNSLQSLDI FTHDGGKGSL FWLLDHTRTS
460 470 480 490 500
FGLRMLREWI LKPLVDVHQI EERLDAIECI TSEINNSIFF ESLNQMLNHT
510 520 530 540 550
PDLLRTLNRI MYGTTSRKEV YFYLKQITSF VDHFKMHQSY LSEHFKSSDG
560 570 580 590 600
RIGKQSPLLF RLFSELNELL STTQLPHFLT MINVSAVMEK NSDKQVMDFF
610 620 630 640 650
NLNNYDCSEG IIKIQRESES VRSQLKEELA EIRKYLKRPY LNFRDEVDYL
660 670 680 690 700
IEVKNSQIKD LPDDWIKVNN TKMVSRFTTP RTQKLTQKLE YYKDLLIRES
710 720 730 740 750
ELQYKEFLNK ITAEYTELRK ITLNLAQYDC ILSLAATSCN VNYVRPTFVN
760 770 780 790 800
GQQAIIAKNA RNPIIESLDV HYVPNDIMMS PENGKINIIT GPNMGGKSSY
810 820 830 840 850
IRQVALLTIM AQIGSFVPAE EIRLSIFENV LTRIGAHDDI INGDSTFKVE
860 870 880 890 900
MLDILHILKN CNKRSLLLLD EVGRGTGTHD GIAISYALIK YFSELSDCPL
910 920 930 940 950
ILFTTHFPML GEIKSPLIRN YHMDYVEEQK TGEDWMSVIF LYKLKKGLTY
960 970 980 990 1000
NSYGMNVAKL ARLDKDIINR AFSISEELRK ESINEDALKL FSSLKRILKS
1010
DNITATDKLA KLLSLDIH
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64954 Genomic DNA Translation: CAA46116.1 Different initiation. M96250 Genomic DNA Translation: AAA34803.1 Different initiation. X59720 Genomic DNA Translation: CAA42247.1 Different initiation. BK006937 Genomic DNA Translation: DAA07561.1 |
PIRi | S19508 |
RefSeqi | NP_010016.2, NM_001178798.1 |
Genome annotation databases
EnsemblFungii | YCR092C_mRNA; YCR092C; YCR092C |
GeneIDi | 850454 |
KEGGi | sce:YCR092C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64954 Genomic DNA Translation: CAA46116.1 Different initiation. M96250 Genomic DNA Translation: AAA34803.1 Different initiation. X59720 Genomic DNA Translation: CAA42247.1 Different initiation. BK006937 Genomic DNA Translation: DAA07561.1 |
PIRi | S19508 |
RefSeqi | NP_010016.2, NM_001178798.1 |
3D structure databases
AlphaFoldDBi | P25336 |
SMRi | P25336 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31064, 97 interactors |
ComplexPortali | CPX-1036, DNA mismatch repair MutSbeta complex |
DIPi | DIP-2422N |
ELMi | P25336 |
IntActi | P25336, 36 interactors |
MINTi | P25336 |
STRINGi | 4932.YCR092C |
Proteomic databases
MaxQBi | P25336 |
PaxDbi | P25336 |
PRIDEi | P25336 |
Genome annotation databases
EnsemblFungii | YCR092C_mRNA; YCR092C; YCR092C |
GeneIDi | 850454 |
KEGGi | sce:YCR092C |
Organism-specific databases
SGDi | S000000688, MSH3 |
VEuPathDBi | FungiDB:YCR092C |
Phylogenomic databases
eggNOGi | KOG0218, Eukaryota |
GeneTreei | ENSGT00550000074949 |
HOGENOMi | CLU_002472_3_1_1 |
InParanoidi | P25336 |
OMAi | LRNVHMK |
Enzyme and pathway databases
Reactomei | R-SCE-5358606, Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) |
Miscellaneous databases
PROi | PR:P25336 |
RNActi | P25336, protein |
Family and domain databases
Gene3Di | 3.30.420.110, 1 hit 3.40.1170.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR007695, DNA_mismatch_repair_MutS-lik_N IPR017261, DNA_mismatch_repair_MutS/MSH IPR000432, DNA_mismatch_repair_MutS_C IPR007861, DNA_mismatch_repair_MutS_clamp IPR007696, DNA_mismatch_repair_MutS_core IPR016151, DNA_mismatch_repair_MutS_N IPR036187, DNA_mismatch_repair_MutS_sf IPR007860, DNA_mmatch_repair_MutS_con_dom IPR036678, MutS_con_dom_sf IPR045076, MutS_family IPR027417, P-loop_NTPase |
PANTHERi | PTHR11361, PTHR11361, 1 hit |
Pfami | View protein in Pfam PF01624, MutS_I, 1 hit PF05188, MutS_II, 1 hit PF05192, MutS_III, 1 hit PF05190, MutS_IV, 1 hit PF00488, MutS_V, 1 hit |
PIRSFi | PIRSF037677, DNA_mis_repair_Msh6, 1 hit |
SMARTi | View protein in SMART SM00534, MUTSac, 1 hit SM00533, MUTSd, 1 hit |
SUPFAMi | SSF48334, SSF48334, 1 hit SSF52540, SSF52540, 1 hit SSF55271, SSF55271, 1 hit |
PROSITEi | View protein in PROSITE PS00486, DNA_MISMATCH_REPAIR_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MSH3_YEAST | |
Accessioni | P25336Primary (citable) accession number: P25336 Secondary accession number(s): D6VR92 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | June 10, 2008 | |
Last modified: | May 25, 2022 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome III
Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names - SIMILARITY comments
Index of protein domains and families