MSH3

Functionⁱ

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. MSH3 provides substrate-binding and substrate specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with either the MutL alpha or MutL beta heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. MutS beta also has a role in regulation of heteroduplex formation during mitotic and meiotic recombination. MutS beta binds to DNA flap structures predicted to form during recombination, and is required for 3' non-homologous tail removal (NHTR). MutS beta-binding alters the DNA conformation of its substrate at the ds/ssDNA junction and may facilitate its recognition and/or cleavage by the downstream nucleotide excision repair (NER) RAD1-RAD10 endonuclease. ATP binding and hydrolysis play a pivotal role in MMR and NHTR.10 Publications

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	791 – 798	ATPSequence analysis		8

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
damaged DNA binding Source: GO_Central
DNA-dependent ATPase activity Source: GO_Central
DNA insertion or deletion binding
Source: SGD
Inferred from direct assayⁱ
- 8805366
double-strand/single-strand DNA junction binding
Source: SGD
Inferred from direct assayⁱ
- 16781730

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA recombination
Source: SGD
Inferred from mutant phenotypeⁱ
- 15920474
maintenance of DNA repeat elements Source: GO_Central
meiotic mismatch repair
Source: SGD
Inferred from mutant phenotypeⁱ
- 16702432
mismatch repair
Source: SGD
Inferred from mutant phenotypeⁱ
- 8510668
- 9520271
mitotic recombination
Source: SGD
Inferred from mutant phenotypeⁱ
- 8849883
removal of nonhomologous ends
Source: SGD
Inferred from mutant phenotypeⁱ
- 9256462
replication fork arrest
Source: SGD
Inferred from mutant phenotypeⁱ
- 27045900

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	DNA damage, DNA repair
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29386-MONOMER
Reactomeⁱ	R-SCE-5358606 Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta)

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA mismatch repair protein MSH3 Alternative name(s): Mismatch-binding protein Short name: MBP MutS protein homolog 3
Gene namesⁱ	Name:MSH3 Ordered Locus Names:YCR092C ORF Names:YCR1152, YCR92C
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome III

Organism-specific databases

EuPathDBⁱ	FungiDB:YCR092C
Saccharomyces Genome Database More...SGDⁱ	S000000688 MSH3

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	4	Q → A: Partially functional in a mismatch repair assay; when associated with 10-AA-11. 1 Publication	1
Mutagenesisⁱ	10 – 11	FF → AA: Partially functional in a mismatch repair assay; when associated with A-4. 1 Publication	2
Mutagenesisⁱ	158	K → A: Alters DNA-binding activity and impairs MSH2-MSH3-mediated DNA mismatch repair; when associated with ALA-160. 1 Publication	1
Mutagenesisⁱ	160	K → A: Alters DNA-binding activity and impairs MSH2-MSH3-mediated DNA mismatch repair; when associated with ALA-158. 1 Publication	1
Mutagenesisⁱ	203	P → A: No effect. 1 Publication	1
Mutagenesisⁱ	226	Q → A: No effect. 1 Publication	1
Mutagenesisⁱ	247	R → A: Impairs MSH2-MSH3-mediated DNA mismatch repair. 1 Publication	1
Mutagenesisⁱ	796	G → D: Defective in MMR and in NHTR. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000115195	1 – 1018	DNA mismatch repair protein MSH3Add BLAST		1018

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P25336
PaxDbⁱ	P25336
PRIDEⁱ	P25336

Interactionⁱ

Subunit structureⁱ

Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with either MutL alpha (MLH1-PMS1) or MutL beta (MLH1-MLH3). MutS beta interacts with proliferating cell nuclear antigen (PCNA/POL30). Interacts with SAW1.4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
MSH2	P25847	3	EBI-11362,EBI-11352

With

Entry

#Exp.

IntAct

Notes

MSH2

P25847

3

EBI-11362,EBI-11352

Protein-protein interaction databases

BioGridⁱ	31064, 88 interactors
ComplexPortalⁱ	CPX-1036 DNA mismatch repair MutSbeta complex
Database of interacting proteins More...DIPⁱ	DIP-2422N
ELMⁱ	P25336
IntActⁱ	P25336, 36 interactors
Molecular INTeraction database More...MINTⁱ	P25336
STRINGⁱ	4932.YCR092C

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P25336
SMRⁱ	P25336
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	126 – 256	Mispair-binding domainAdd BLAST		131

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	4 – 11	PIP box		8

Domainⁱ

The PIP box serves as a PCNA(POL30)-recognition and -binding motif.

Sequence similaritiesⁱ

Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.Curated

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074949
HOGENOMⁱ	HOG000057130
InParanoidⁱ	P25336
KEGG Orthology (KO) More...KOⁱ	K08736
OMAⁱ	YHLKSEF
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C0EWT

Family and domain databases

Gene3Dⁱ	3.30.420.110, 1 hit 3.40.1170.10, 1 hit
InterProⁱ	View protein in InterPro IPR007695 DNA_mismatch_repair_MutS-lik_N IPR017261 DNA_mismatch_repair_MutS/MSH IPR000432 DNA_mismatch_repair_MutS_C IPR007861 DNA_mismatch_repair_MutS_clamp IPR007696 DNA_mismatch_repair_MutS_core IPR016151 DNA_mismatch_repair_MutS_N IPR036187 DNA_mismatch_repair_MutS_sf IPR007860 DNA_mmatch_repair_MutS_con_dom IPR036678 MutS_con_dom_sf IPR027417 P-loop_NTPase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01624 MutS_I, 1 hit PF05188 MutS_II, 1 hit PF05192 MutS_III, 1 hit PF05190 MutS_IV, 1 hit PF00488 MutS_V, 1 hit
PIRSFⁱ	PIRSF037677 DNA_mis_repair_Msh6, 1 hit
SMARTⁱ	View protein in SMART SM00534 MUTSac, 1 hit SM00533 MUTSd, 1 hit
SUPFAMⁱ	SSF48334 SSF48334, 1 hit SSF52540 SSF52540, 1 hit SSF55271 SSF55271, 1 hit
PROSITEⁱ	View protein in PROSITE PS00486 DNA_MISMATCH_REPAIR_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P25336-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAGQPTISRF FKKAVKSELT HKQEQEVAVG NGAGSESICL DTDEEDNLSS 
        60         70         80         90        100
VASTTVTNDS FPLKGSVSSK NSKNSEKTSG TSTTFNDIDF AKKLDRIMKR 
       110        120        130        140        150
RSDENVEAED DEEEGEEDFV KKKARKSPTA KLTPLDKQVK DLKMHHRDKV 
       160        170        180        190        200
LVIRVGYKYK CFAEDAVTVS RILHIKLVPG KLTIDESNPQ DCNHRQFAYC 
       210        220        230        240        250
SFPDVRLNVH LERLVHHNLK VAVVEQAETS AIKKHDPGAS KSSVFERKIS 
       260        270        280        290        300
NVFTKATFGV NSTFVLRGKR ILGDTNSIWA LSRDVHQGKV AKYSLISVNL 
       310        320        330        340        350
NNGEVVYDEF EEPNLADEKL QIRIKYLQPI EVLVNTDDLP LHVAKFFKDI 
       360        370        380        390        400
SCPLIHKQEY DLEDHVVQAI KVMNEKIQLS PSLIRLVSKL YSHMVEYNNE 
       410        420        430        440        450
QVMLIPSIYS PFASKIHMLL DPNSLQSLDI FTHDGGKGSL FWLLDHTRTS 
       460        470        480        490        500
FGLRMLREWI LKPLVDVHQI EERLDAIECI TSEINNSIFF ESLNQMLNHT 
       510        520        530        540        550
PDLLRTLNRI MYGTTSRKEV YFYLKQITSF VDHFKMHQSY LSEHFKSSDG 
       560        570        580        590        600
RIGKQSPLLF RLFSELNELL STTQLPHFLT MINVSAVMEK NSDKQVMDFF 
       610        620        630        640        650
NLNNYDCSEG IIKIQRESES VRSQLKEELA EIRKYLKRPY LNFRDEVDYL 
       660        670        680        690        700
IEVKNSQIKD LPDDWIKVNN TKMVSRFTTP RTQKLTQKLE YYKDLLIRES 
       710        720        730        740        750
ELQYKEFLNK ITAEYTELRK ITLNLAQYDC ILSLAATSCN VNYVRPTFVN 
       760        770        780        790        800
GQQAIIAKNA RNPIIESLDV HYVPNDIMMS PENGKINIIT GPNMGGKSSY 
       810        820        830        840        850
IRQVALLTIM AQIGSFVPAE EIRLSIFENV LTRIGAHDDI INGDSTFKVE 
       860        870        880        890        900
MLDILHILKN CNKRSLLLLD EVGRGTGTHD GIAISYALIK YFSELSDCPL 
       910        920        930        940        950
ILFTTHFPML GEIKSPLIRN YHMDYVEEQK TGEDWMSVIF LYKLKKGLTY 
       960        970        980        990       1000
NSYGMNVAKL ARLDKDIINR AFSISEELRK ESINEDALKL FSSLKRILKS 
      1010 
DNITATDKLA KLLSLDIH

Length:1,018

Mass (Da):116,534

Last modified:June 10, 2008 - v2

Checksum:ⁱ1AD91C2E2F2856EF

GO

Sequence cautionⁱ

The sequence AAA34803 differs from that shown. Reason: Erroneous initiation.Curated

The sequence CAA42247 differs from that shown. Reason: Erroneous initiation.Curated

The sequence CAA46116 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X64954 Genomic DNA Translation: CAA46116.1 Different initiation. M96250 Genomic DNA Translation: AAA34803.1 Different initiation. X59720 Genomic DNA Translation: CAA42247.1 Different initiation. BK006937 Genomic DNA Translation: DAA07561.1
PIRⁱ	S19508
NCBI Reference Sequences More...RefSeqⁱ	NP_010016.2, NM_001178798.1

Genome annotation databases

EnsemblFungiⁱ	CAA42247; CAA42247; CAA42247 YCR092C; YCR092C; YCR092C
GeneIDⁱ	850454
KEGGⁱ	sce:YCR092C

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P25336	DNA mismatch repair protein MSH3		YEAS7		1018	UniRef100_A6ZTR3

Protein	Similar proteins		Species	Score	Length	Source
P25336	DNA mismatch repair protein MSH3		YEAS7		1018	UniRef90_A6ZTR3
Mismatch repair protein		YEAS1		1047
K7_Msh3p		YEASK		1047
MSH3p Mismatch repair protein		Saccharomyces sp. 'boulardii'		1047
YCR092Cp-like protein		YEAS6		1047
+3

Protein	Similar proteins		Species	Score	Length	Source
P25336	DNA mismatch repair protein MSH3		YEAS7		1018	UniRef50_A6ZTR3
K7_Msh3p		YEASK		1047
YCR092Cp-like protein		YEAS6		1047
MSH3p Mismatch repair protein		Saccharomyces sp. 'boulardii'		1047
Mismatch repair protein		YEAS1		1047
+4

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X64954 Genomic DNA Translation: CAA46116.1 Different initiation. M96250 Genomic DNA Translation: AAA34803.1 Different initiation. X59720 Genomic DNA Translation: CAA42247.1 Different initiation. BK006937 Genomic DNA Translation: DAA07561.1
PIRⁱ	S19508
RefSeqⁱ	NP_010016.2, NM_001178798.1

3D structure databases

ProteinModelPortalⁱ	P25336
SMRⁱ	P25336
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	31064, 88 interactors
ComplexPortalⁱ	CPX-1036 DNA mismatch repair MutSbeta complex
DIPⁱ	DIP-2422N
ELMⁱ	P25336
IntActⁱ	P25336, 36 interactors
MINTⁱ	P25336
STRINGⁱ	4932.YCR092C

Proteomic databases

MaxQBⁱ	P25336
PaxDbⁱ	P25336
PRIDEⁱ	P25336

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	CAA42247; CAA42247; CAA42247 YCR092C; YCR092C; YCR092C
GeneIDⁱ	850454
KEGGⁱ	sce:YCR092C

Organism-specific databases

EuPathDBⁱ	FungiDB:YCR092C
SGDⁱ	S000000688 MSH3

Phylogenomic databases

GeneTreeⁱ	ENSGT00550000074949
HOGENOMⁱ	HOG000057130
InParanoidⁱ	P25336
KOⁱ	K08736
OMAⁱ	YHLKSEF
OrthoDBⁱ	EOG092C0EWT

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29386-MONOMER
Reactomeⁱ	R-SCE-5358606 Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta)

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P25336

PROⁱ

PR:P25336

Family and domain databases

Gene3Dⁱ	3.30.420.110, 1 hit 3.40.1170.10, 1 hit
InterProⁱ	View protein in InterPro IPR007695 DNA_mismatch_repair_MutS-lik_N IPR017261 DNA_mismatch_repair_MutS/MSH IPR000432 DNA_mismatch_repair_MutS_C IPR007861 DNA_mismatch_repair_MutS_clamp IPR007696 DNA_mismatch_repair_MutS_core IPR016151 DNA_mismatch_repair_MutS_N IPR036187 DNA_mismatch_repair_MutS_sf IPR007860 DNA_mmatch_repair_MutS_con_dom IPR036678 MutS_con_dom_sf IPR027417 P-loop_NTPase
Pfamⁱ	View protein in Pfam PF01624 MutS_I, 1 hit PF05188 MutS_II, 1 hit PF05192 MutS_III, 1 hit PF05190 MutS_IV, 1 hit PF00488 MutS_V, 1 hit
PIRSFⁱ	PIRSF037677 DNA_mis_repair_Msh6, 1 hit
SMARTⁱ	View protein in SMART SM00534 MUTSac, 1 hit SM00533 MUTSd, 1 hit
SUPFAMⁱ	SSF48334 SSF48334, 1 hit SSF52540 SSF52540, 1 hit SSF55271 SSF55271, 1 hit
PROSITEⁱ	View protein in PROSITE PS00486 DNA_MISMATCH_REPAIR_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MSH3_YEAST
Accessionⁱ	P25336Primary (citable) accession number: P25336 Secondary accession number(s): D6VR92
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
	Last sequence update:	June 10, 2008
	Last modified:	September 12, 2018
	This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome III
Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

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UniProtKB - P25336 (MSH3_YEAST)

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DNA mismatch repair protein MSH3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ