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Protein

DnaJ-related protein SCJ1

Gene

SCJ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates protein folding in the endoplasmic reticulum lumen. Probably acts as a J-protein for the Hsp70-type chaperone KAR2 by stimulating its ATP-dependent reaction cycle and initiating folding reactions. Also involved in the endoplasmic reticulum-associated degradation (ERAD) process. Cooperates with KAR2 and another J-protein JEM1 to facilitate the export of ERAD substrates to the cytoplasm by maintaining them in a translocation-competent state and preventing their aggregation in the endoplasmic reticulum lumen.2 Publications

Miscellaneous

Present with 8260 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri156 – 237CR-typePROSITE-ProRule annotationAdd BLAST82

GO - Molecular functioni

  • chaperone binding Source: SGD
  • heat shock protein binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • unfolded protein binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32897-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ-related protein SCJ1
Short name:
J protein SCJ1
Gene namesi
Name:SCJ1
Ordered Locus Names:YMR214W
ORF Names:YM8261.08
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

SGDiS000004827 SCJ1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000007109122 – 377DnaJ-related protein SCJ1Add BLAST356

Proteomic databases

MaxQBiP25303
PaxDbiP25303
PRIDEiP25303

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi35392, 153 interactors
DIPiDIP-2395N
IntActiP25303, 36 interactors
MINTiP25303
STRINGi4932.YMR214W

Structurei

3D structure databases

ProteinModelPortaliP25303
SMRiP25303
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 88JPROSITE-ProRule annotationAdd BLAST66
Repeati169 – 176CXXCXGXG motif8
Repeati185 – 192CXXCXGXG motif8
Repeati211 – 218CXXCXGXG motif8
Repeati225 – 232CXXCXGXG motif8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi288 – 290Cell attachment siteSequence analysis3
Motifi374 – 377Prevents secretion from ERPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi95 – 132Gly-richAdd BLAST38

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri156 – 237CR-typePROSITE-ProRule annotationAdd BLAST82

Keywords - Domaini

Repeat, Signal, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00860000133842
HOGENOMiHOG000226718
InParanoidiP25303
KOiK14002
OMAiHCRGTGA
OrthoDBiEOG092C1UGR

Family and domain databases

CDDicd06257 DnaJ, 1 hit
cd10719 DnaJ_zf, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR001305 HSP_DnaJ_Cys-rich_dom
IPR036410 HSP_DnaJ_Cys-rich_dom_sf
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PF00684 DnaJ_CXXCXGXG, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 2 hits
SSF57938 SSF57938, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
PS00014 ER_TARGET, 1 hit
PS51188 ZF_CR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25303-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIPKLYIHLI LSLLLLPLIL AQDYYAILEI DKDATEKEIK SAYRQLSKKY
60 70 80 90 100
HPDKNAGSEE AHQKFIEVGE AYDVLSDPEK KKIYDQFGAD AVKNGGGGGG
110 120 130 140 150
PGGPGAGGFH DPFDIFERMF QGGHGGPGGG FGQRQRQRGP MIKVQEKLSL
160 170 180 190 200
KQFYSGSSIE FTLNLNDECD ACHGSGSADG KLAQCPDCQG RGVIIQVLRM
210 220 230 240 250
GIMTQQIQQM CGRCGGTGQI IKNECKTCHG KKVTKKNKFF HVDVPPGAPR
260 270 280 290 300
NYMDTRVGEA EKGPDFDAGD LVIEFKEKDT ENMGYRRRGD NLYRTEVLSA
310 320 330 340 350
AEALYGGWQR TIEFLDENKP VKLSRPAHVV VSNGEVEVVK GFGMPKGSKG
360 370
YGDLYIDYVV VMPKTFKSGQ NMLKDEL
Length:377
Mass (Da):41,543
Last modified:September 5, 2006 - v2
Checksum:iFD0226902F33E2B8
GO

Sequence cautioni

The sequence CAA41529 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA89929 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58679 Genomic DNA Translation: CAA41529.1 Different initiation.
Z49809 Genomic DNA Translation: CAA89929.1 Different initiation.
BK006946 Genomic DNA Translation: DAA10113.1
PIRiS13648
RefSeqiNP_013941.2, NM_001182721.1

Genome annotation databases

EnsemblFungiiYMR214W; YMR214W; YMR214W
GeneIDi855254
KEGGisce:YMR214W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58679 Genomic DNA Translation: CAA41529.1 Different initiation.
Z49809 Genomic DNA Translation: CAA89929.1 Different initiation.
BK006946 Genomic DNA Translation: DAA10113.1
PIRiS13648
RefSeqiNP_013941.2, NM_001182721.1

3D structure databases

ProteinModelPortaliP25303
SMRiP25303
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35392, 153 interactors
DIPiDIP-2395N
IntActiP25303, 36 interactors
MINTiP25303
STRINGi4932.YMR214W

Proteomic databases

MaxQBiP25303
PaxDbiP25303
PRIDEiP25303

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR214W; YMR214W; YMR214W
GeneIDi855254
KEGGisce:YMR214W

Organism-specific databases

SGDiS000004827 SCJ1

Phylogenomic databases

GeneTreeiENSGT00860000133842
HOGENOMiHOG000226718
InParanoidiP25303
KOiK14002
OMAiHCRGTGA
OrthoDBiEOG092C1UGR

Enzyme and pathway databases

BioCyciYEAST:G3O-32897-MONOMER

Miscellaneous databases

PROiPR:P25303

Family and domain databases

CDDicd06257 DnaJ, 1 hit
cd10719 DnaJ_zf, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR001305 HSP_DnaJ_Cys-rich_dom
IPR036410 HSP_DnaJ_Cys-rich_dom_sf
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PF00684 DnaJ_CXXCXGXG, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 2 hits
SSF57938 SSF57938, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
PS00014 ER_TARGET, 1 hit
PS51188 ZF_CR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSCJ1_YEAST
AccessioniPrimary (citable) accession number: P25303
Secondary accession number(s): D6W039
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: September 5, 2006
Last modified: November 7, 2018
This is version 181 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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