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Protein

mRNA 3'-end-processing protein RNA15

Gene

RNA15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor NAB4/CFIB and the cleavage and polyadenylation factor (CPF) complex. Binds to A-rich RNA sequence elements.2 Publications

Miscellaneous

Present with 6350 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • mRNA binding Source: SGD
  • protein heterodimerization activity Source: SGD

GO - Biological processi

  • mRNA polyadenylation Source: SGD
  • pre-mRNA cleavage required for polyadenylation Source: SGD
  • response to DNA damage checkpoint signaling Source: SGD

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-30555-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA 3'-end-processing protein RNA15
Gene namesi
Name:RNA15
Ordered Locus Names:YGL044C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL044C
SGDiS000003012 RNA15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000818071 – 296mRNA 3'-end-processing protein RNA15Add BLAST296

Proteomic databases

MaxQBiP25299
PaxDbiP25299
PRIDEiP25299

Interactioni

Subunit structurei

Component of the CFIA complex, which is composed of RNA14, RNA15, PCF11 and CLP1. Interacts directly with RNA14. Interacts with polyadenylate-binding protein PAB1.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein heterodimerization activity Source: SGD

Protein-protein interaction databases

BioGridi33204, 315 interactors
ComplexPortaliCPX-1895 mRNA cleavage factor complex CF IA
CPX-1896 mRNA cleavage factor complex CF I
DIPiDIP-1489N
IntActiP25299, 13 interactors
MINTiP25299
STRINGi4932.YGL044C

Structurei

Secondary structure

1296
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 24Combined sources7
Helixi31 – 39Combined sources9
Beta strandi44 – 48Combined sources5
Turni53 – 55Combined sources3
Beta strandi60 – 68Combined sources9
Helixi69 – 79Combined sources11
Beta strandi85 – 87Combined sources3
Beta strandi90 – 93Combined sources4
Helixi99 – 101Combined sources3
Helixi102 – 106Combined sources5
Helixi139 – 143Combined sources5
Turni156 – 158Combined sources3
Helixi161 – 170Combined sources10
Helixi174 – 190Combined sources17
Helixi192 – 201Combined sources10
Helixi203 – 216Combined sources14
Beta strandi217 – 219Combined sources3
Helixi221 – 224Combined sources4
Turni225 – 227Combined sources3

3D structure databases

ProteinModelPortaliP25299
SMRiP25299
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25299

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 96RRMPROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi104 – 109Poly-Gln6
Compositional biasi111 – 128Asn-richAdd BLAST18

Phylogenomic databases

GeneTreeiENSGT00890000139457
HOGENOMiHOG000247912
KOiK14407
OrthoDBiEOG092C3SCB

Family and domain databases

Gene3Di1.10.20.70, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR038192 CSTF2_C_sf
IPR026896 CSTF_C
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF14304 CSTF_C, 1 hit
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequencei

Sequence statusi: Complete.

P25299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRQSGVNAG VQNNPPSRVV YLGSIPYDQT EEQILDLCSN VGPVINLKMM
60 70 80 90 100
FDPQTGRSKG YAFIEFRDLE SSASAVRNLN GYQLGSRFLK CGYSSNSDIS
110 120 130 140 150
GVSQQQQQQY NNINGNNNNN GNNNNNSNGP DFQNSGNANF LSQKFPELPS
160 170 180 190 200
GIDVNINMTT PAMMISSELA KKPKEVQLKF LQKFQEWTRA HPEDAVSLLE
210 220 230 240 250
LCPQLSFVTA ELLLTNGICK VDDLIPLASR PQEEASATNN NSVNEVVDPA
260 270 280 290
VLNKQKELLK QVLQLNDSQI SILPDDERMA IWDLKQKALR GEFGAF
Length:296
Mass (Da):32,791
Last modified:May 1, 1992 - v1
Checksum:i10053B8648A71ECB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73462 Genomic DNA Translation: AAA34984.1
Z72566 Genomic DNA Translation: CAA96746.1
BK006941 Genomic DNA Translation: DAA08056.1
PIRiB40257
RefSeqiNP_011471.1, NM_001180909.1

Genome annotation databases

EnsemblFungiiYGL044C; YGL044C; YGL044C
GeneIDi852838
KEGGisce:YGL044C

Similar proteinsi

Entry informationi

Entry nameiRNA15_YEAST
AccessioniPrimary (citable) accession number: P25299
Secondary accession number(s): D6VU95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: July 18, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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