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Protein

Protein SIS1

Gene

SIS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for nuclear migration during mitosis. It is required for the normal initiation of translation. Might mediate the dissociation of a specific protein complex of the translation machinery. Essential for viability.

Miscellaneous

Present with 20300 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • misfolded protein binding Source: SGD
  • unfolded protein binding Source: InterPro

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • misfolded protein transport Source: SGD
  • nuclear protein quality control by the ubiquitin-proteasome system Source: SGD
  • protein folding Source: SGD
  • translational initiation Source: SGD
  • tRNA import into nucleus Source: SGD

Keywordsi

Molecular functionChaperone, DNA-binding
Biological processCell cycle

Enzyme and pathway databases

BioCyciYEAST:G3O-33049-MONOMER
ReactomeiR-SCE-3371453 Regulation of HSF1-mediated heat shock response
R-SCE-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-SCE-3371568 Attenuation phase
R-SCE-3371571 HSF1-dependent transactivation
R-SCE-5687128 MAPK6/MAPK4 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SIS1
Gene namesi
Name:SIS1
Ordered Locus Names:YNL007C
ORF Names:N2879
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL007C
SGDiS000004952 SIS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000710901 – 352Protein SIS1Add BLAST352

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei275PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25294
PaxDbiP25294
PRIDEiP25294

PTM databases

iPTMnetiP25294

Interactioni

Subunit structurei

Interacts with polyadenylate-binding protein PAB1.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • misfolded protein binding Source: SGD
  • unfolded protein binding Source: InterPro

Protein-protein interaction databases

BioGridi35818, 94 interactors
DIPiDIP-4385N
IntActiP25294, 551 interactors
MINTiP25294
STRINGi4932.YNL007C

Structurei

Secondary structure

1352
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 11Combined sources6
Helixi19 – 33Combined sources15
Helixi42 – 55Combined sources14
Helixi58 – 67Combined sources10
Helixi69 – 73Combined sources5
Beta strandi181 – 188Combined sources8
Helixi190 – 195Combined sources6
Beta strandi198 – 207Combined sources10
Turni208 – 210Combined sources3
Beta strandi211 – 220Combined sources10
Beta strandi230 – 235Combined sources6
Beta strandi237 – 239Combined sources3
Beta strandi241 – 244Combined sources4
Beta strandi248 – 255Combined sources8
Beta strandi259 – 264Combined sources6
Beta strandi267 – 272Combined sources6
Helixi276 – 281Combined sources6
Beta strandi283 – 288Combined sources6
Beta strandi290 – 292Combined sources3
Beta strandi294 – 301Combined sources8
Beta strandi308 – 310Combined sources3
Beta strandi319 – 321Combined sources3
Beta strandi328 – 332Combined sources5
Helixi344 – 347Combined sources4

3D structure databases

ProteinModelPortaliP25294
SMRiP25294
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25294

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 70JPROSITE-ProRule annotationAdd BLAST67

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 166Gly-richAdd BLAST92

Phylogenomic databases

GeneTreeiENSGT00760000118947
HOGENOMiHOG000226718
InParanoidiP25294
KOiK09510
OMAiKEGMMDH
OrthoDBiEOG092C1UGR

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 2 hits
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit

Sequencei

Sequence statusi: Complete.

P25294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKETKLYDL LGVSPSANEQ ELKKGYRKAA LKYHPDKPTG DTEKFKEISE
60 70 80 90 100
AFEILNDPQK REIYDQYGLE AARSGGPSFG PGGPGGAGGA GGFPGGAGGF
110 120 130 140 150
SGGHAFSNED AFNIFSQFFG GSSPFGGADD SGFSFSSYPS GGGAGMGGMP
160 170 180 190 200
GGMGGMHGGM GGMPGGFRSA SSSPTYPEEE TVQVNLPVSL EDLFVGKKKS
210 220 230 240 250
FKIGRKGPHG ASEKTQIDIQ LKPGWKAGTK ITYKNQGDYN PQTGRRKTLQ
260 270 280 290 300
FVIQEKSHPN FKRDGDDLIY TLPLSFKESL LGFSKTIQTI DGRTLPLSRV
310 320 330 340 350
QPVQPSQTST YPGQGMPTPK NPSQRGNLIV KYKVDYPISL NDAQKRAIDE

NF
Length:352
Mass (Da):37,590
Last modified:May 1, 1992 - v1
Checksum:i642D3709C0FD5682
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58460 Genomic DNA Translation: CAA41366.1
Z71283 Genomic DNA Translation: CAA95866.1
BK006947 Genomic DNA Translation: DAA10536.1
PIRiA39660
RefSeqiNP_014391.1, NM_001182846.1

Genome annotation databases

EnsemblFungiiYNL007C; YNL007C; YNL007C
GeneIDi855725
KEGGisce:YNL007C

Similar proteinsi

Entry informationi

Entry nameiSIS1_YEAST
AccessioniPrimary (citable) accession number: P25294
Secondary accession number(s): D6W1H0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: July 18, 2018
This is version 182 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

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