UniProtKB - P25240 (MT57_ECOLX)
Protein
Modification methylase Eco57IB
Gene
eco57IBM
Organism
Escherichia coli
Status
Functioni
This methylase recognizes the double-stranded sequence 5'-CTGAAG-3' in one strand and 5'-CTTCAG-3' in the other, causes specific methylation on A-5 on one strand, and protects the DNA from cleavage by the Eco57I endonuclease.
Catalytic activityi
- a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H+ + S-adenosyl-L-homocysteineEC:2.1.1.72
GO - Molecular functioni
- DNA binding Source: InterPro
- N-methyltransferase activity Source: InterPro
- site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
GO - Biological processi
- DNA restriction-modification system Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Restriction system |
Ligand | S-adenosyl-L-methionine |
Protein family/group databases
REBASEi | 5234, M.Eco57I |
Names & Taxonomyi
Protein namesi | Recommended name: Modification methylase Eco57IB (EC:2.1.1.72)Short name: M.Eco57IB Alternative name(s): Adenine-specific methyltransferase Eco57IB |
Gene namesi | Name:eco57IBM Synonyms:eco57IM |
Organismi | Escherichia coli |
Taxonomic identifieri | 562 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087940 | 1 – 544 | Modification methylase Eco57IBAdd BLAST | 544 |
Interactioni
Subunit structurei
Monomer.
Family & Domainsi
Sequence similaritiesi
Belongs to the N(4)/N(6)-methyltransferase family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR003356, DNA_methylase_A-5 IPR002052, DNA_methylase_N6_adenine_CS IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF02384, N6_Mtase, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P25240-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFKADQTSQ KLRGGYYTPQ NLADYVTKWV LSKNPKTILE PSCGDGVFIQ
60 70 80 90 100
AIANNGYNSN IELFCFELFD TEASKALERC KLNNFSNATI TEGDFLVWAN
110 120 130 140 150
ECLKKNKQIF DGALGNPPFI RYQFLERNFQ EQAQLVFEHL DLKFTKHTNA
160 170 180 190 200
WVPFLLSSLA LLKQGGRIGM VIPSEISHVM HAQSLRSYLG HVCSKIVIID
210 220 230 240 250
PKEIWFEDTL QGAVILLAEK KQYPDEASQG VGIVSVSGFE FLQEDPNVLF
260 270 280 290 300
NDTAGINGET VEGKWTKATL SIDELQLIKR VIAHPDVRKF KDIAKVDVGR
310 320 330 340 350
YCDGANNYFL VDNETVKLYK LERFAHPMFG RSQHCPGIIY DEKQHIENQE
360 370 380 390 400
KGLPTNFLYI DEEFEYLSKS VKNYIKLGEV EEYHKRYKCR IRKPWFKVPS
410 420 430 440 450
VYSTEIGMLK RCHDAPRLIH NRVRAYTTDT AYRVSSTVTS TENLVCSFLN
460 470 480 490 500
PITVITAELE GLFYGGGVLE LVPSEIEKLY ILIVEGLEHN VEELNLLIKD
510 520 530 540
GQIERVIRQQ GSLILGTLGF TQEENEKLVE IGRSLEIEGY VSRV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74821 Genomic DNA Translation: AAA23388.1 X61122 Genomic DNA Translation: CAA43433.1 |
PIRi | S26425 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74821 Genomic DNA Translation: AAA23388.1 X61122 Genomic DNA Translation: CAA43433.1 |
PIRi | S26425 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein family/group databases
REBASEi | 5234, M.Eco57I |
Miscellaneous databases
PROi | PR:P25240 |
Family and domain databases
InterProi | View protein in InterPro IPR003356, DNA_methylase_A-5 IPR002052, DNA_methylase_N6_adenine_CS IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF02384, N6_Mtase, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MT57_ECOLX | |
Accessioni | P25240Primary (citable) accession number: P25240 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | May 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 78 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families - Restriction enzymes and methylases
Classification of restriction enzymes and methylases and list of entries