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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

Gene

Rpn2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • ribosome binding Source: RGD

GO - Biological processi

  • aging Source: RGD
  • protein N-linked glycosylation Source: GO_Central
  • response to drug Source: RGD

Enzyme and pathway databases

UniPathwayi
UPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
Ribophorin II
Short name:
RPN-II
Ribophorin-2
Gene namesi
Name:Rpn2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi62075 Rpn2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 540LumenalSequence analysisAdd BLAST518
Transmembranei541 – 561HelicalSequence analysisAdd BLAST21
Topological domaini562 – 571CytoplasmicSequence analysis10
Transmembranei572 – 592HelicalSequence analysisAdd BLAST21
Topological domaini593 – 596LumenalSequence analysis4
Transmembranei597 – 617HelicalSequence analysisAdd BLAST21
Topological domaini618 – 631CytoplasmicSequence analysisAdd BLAST14

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000002224523 – 631Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2Add BLAST609

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi106N-linked (GlcNAc...) asparagineCurated1
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP25235
PRIDEiP25235

PTM databases

iPTMnetiP25235
PhosphoSitePlusiP25235
SwissPalmiP25235

Expressioni

Gene expression databases

BgeeiENSRNOG00000007492 Expressed in 10 organ(s), highest expression level in liver
ExpressionAtlasiP25235 baseline and differential
GenevisibleiP25235 RN

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).By similarity

Protein-protein interaction databases

BioGridi249196, 2 interactors
IntActiP25235, 3 interactors
MINTiP25235
STRINGi10116.ENSRNOP00000063207

Family & Domainsi

Sequence similaritiesi

Belongs to the SWP1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2447 Eukaryota
ENOG410XRF2 LUCA
GeneTreeiENSGT00390000002635
HOGENOMiHOG000231261
HOVERGENiHBG002365
InParanoidiP25235
KOiK12667
OMAiPKPEIQH
OrthoDBiEOG091G0H3U
PhylomeDBiP25235

Family and domain databases

InterProiView protein in InterPro
IPR008814 Swp1
PANTHERiPTHR12640 PTHR12640, 1 hit
PfamiView protein in Pfam
PF05817 Ribophorin_II, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P25235-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPPGSSAVF LLALTITAST QALTPTHYLT KHDVERLKAS LDRPFTSLES
60 70 80 90 100
AFYSIVGLNS LGAQVPDVKK ACAFIKSNLD PSNVDSLFYA AQSSQVLSGC
110 120 130 140 150
EISVSNETRD LLLAAVSEDS SVAQIYHAVA ALSGFGLPLA SHEALGALTA
160 170 180 190 200
RLSKEETVLA TVQALHTASH LSQQADLRNI VEEIEDLVAR LDELGGVYLQ
210 220 230 240 250
FEEGLELTAL FVAATYKLMD HVGTEPSIKE DQVIQLMNTI FSKKNFESLS
260 270 280 290 300
EAFSVASAAA ALSQNRYHVP VVVVPEGSAS DTQEQAILRL QVSSVLSQPL
310 320 330 340 350
AQAAVKLEHA KSVASRATVL QKMPFSLVGD VFELNFKNVK LPSGYYDFSV
360 370 380 390 400
RVEGDNRYIA NTVELRVKIS TEVGITNVDL STVDKDQSIA PKTTRVTYPA
410 420 430 440 450
KAKGTFIADS HQNFALFFQL VDVNTGAELT PHQTFVRLHN QKTGQEVVFV
460 470 480 490 500
AEPDNKNVYK FELDTSERKI EFDSASGTYT LYLIIGDATL KNPILWNVAD
510 520 530 540 550
VVIKFPEEEA PSTVLSQNLF TPKQEIQHLF REPEKRPPTV VSNTFTALIL
560 570 580 590 600
SPLLLLFALW IRIGANVSNF TFAPSTVIFH LGHAAMLGLM YVYWTQLNMF
610 620 630
QTLKYLAVLG TVTFLAGNRM LAQQAVKRTA H
Length:631
Mass (Da):69,078
Last modified:April 12, 2005 - v2
Checksum:i9BC0486C9C8469CD
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2K757A0A0G2K757_RAT
Dolichyl-diphosphooligosaccharide--...
Rpn2
630Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti298 – 299QP → HA in CAB56805 (PubMed:1710116).Curated2
Sequence conflicti378V → A in CAB56805 (PubMed:1710116).Curated1
Sequence conflicti390A → P in CAB56805 (PubMed:1710116).Curated1
Sequence conflicti459Y → H in CAB56805 (PubMed:1710116).Curated1
Sequence conflicti575 – 577STV → TTI in CAB56805 (PubMed:1710116).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55298 mRNA Translation: CAB56805.1
BC060556 mRNA Translation: AAH60556.1
PIRiJN0065
RefSeqiNP_113886.1, NM_031698.1
UniGeneiRn.2879

Genome annotation databases

EnsembliENSRNOT00000068135; ENSRNOP00000063207; ENSRNOG00000007492
GeneIDi64701
KEGGirno:64701
UCSCiRGD:62075 rat

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55298 mRNA Translation: CAB56805.1
BC060556 mRNA Translation: AAH60556.1
PIRiJN0065
RefSeqiNP_113886.1, NM_031698.1
UniGeneiRn.2879

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249196, 2 interactors
IntActiP25235, 3 interactors
MINTiP25235
STRINGi10116.ENSRNOP00000063207

PTM databases

iPTMnetiP25235
PhosphoSitePlusiP25235
SwissPalmiP25235

Proteomic databases

PaxDbiP25235
PRIDEiP25235

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000068135; ENSRNOP00000063207; ENSRNOG00000007492
GeneIDi64701
KEGGirno:64701
UCSCiRGD:62075 rat

Organism-specific databases

CTDi6185
RGDi62075 Rpn2

Phylogenomic databases

eggNOGiKOG2447 Eukaryota
ENOG410XRF2 LUCA
GeneTreeiENSGT00390000002635
HOGENOMiHOG000231261
HOVERGENiHBG002365
InParanoidiP25235
KOiK12667
OMAiPKPEIQH
OrthoDBiEOG091G0H3U
PhylomeDBiP25235

Enzyme and pathway databases

UniPathwayi
UPA00378

Miscellaneous databases

PROiPR:P25235

Gene expression databases

BgeeiENSRNOG00000007492 Expressed in 10 organ(s), highest expression level in liver
ExpressionAtlasiP25235 baseline and differential
GenevisibleiP25235 RN

Family and domain databases

InterProiView protein in InterPro
IPR008814 Swp1
PANTHERiPTHR12640 PTHR12640, 1 hit
PfamiView protein in Pfam
PF05817 Ribophorin_II, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPN2_RAT
AccessioniPrimary (citable) accession number: P25235
Secondary accession number(s): Q6P9X0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: April 12, 2005
Last modified: November 7, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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