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Protein

Nuclear transcription factor Y subunit beta

Gene

NFYB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi59 – 65By similarity7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-2426168 Activation of gene expression by SREBF (SREBP)
R-HSA-380994 ATF4 activates genes
R-HSA-381183 ATF6 (ATF6-alpha) activates chaperone genes
SignaLinkiP25208
SIGNORiP25208

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transcription factor Y subunit beta
Alternative name(s):
CAAT box DNA-binding protein subunit B
Nuclear transcription factor Y subunit B
Short name:
NF-YB
Gene namesi
Name:NFYB
Synonyms:HAP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000120837.7
HGNCiHGNC:7805 NFYB
MIMi189904 gene
neXtProtiNX_P25208

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4801
OpenTargetsiENSG00000120837
PharmGKBiPA31610

Polymorphism and mutation databases

BioMutaiNFYB
DMDMi399193

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002046091 – 207Nuclear transcription factor Y subunit betaAdd BLAST207

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki140Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Monoubiquitination at Lys-140 plays an important role in transcriptional activation by allowing the deposition of histone H3 methylations as well as histone H2B monoubiquitination at 'Lys-121'.

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP25208
MaxQBiP25208
PaxDbiP25208
PeptideAtlasiP25208
PRIDEiP25208
ProteomicsDBi54266

PTM databases

iPTMnetiP25208
PhosphoSitePlusiP25208

Expressioni

Gene expression databases

BgeeiENSG00000120837 Expressed in 233 organ(s), highest expression level in substantia nigra
CleanExiHS_NFYB
ExpressionAtlasiP25208 baseline and differential
GenevisibleiP25208 HS

Organism-specific databases

HPAiCAB004211

Interactioni

Subunit structurei

Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. Interacts with C1QBP.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110867, 32 interactors
ComplexPortaliCPX-1956 CCAAT-binding factor complex
CORUMiP25208
IntActiP25208, 10 interactors
STRINGi9606.ENSP00000240055

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP25208
SMRiP25208
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25208

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 52A domainAdd BLAST52
Regioni53 – 142B domainAdd BLAST90
Regioni86 – 97Subunit association domain (SAD)By similarityAdd BLAST12
Regioni143 – 207C domainAdd BLAST65

Domaini

Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.

Sequence similaritiesi

Belongs to the NFYB/HAP3 subunit family.Curated

Phylogenomic databases

eggNOGiKOG0869 Eukaryota
COG2036 LUCA
GeneTreeiENSGT00550000074689
HOGENOMiHOG000211832
HOVERGENiHBG008864
InParanoidiP25208
KOiK08065
OMAiKENFREQ
OrthoDBiEOG091G0Y4D
PhylomeDBiP25208
TreeFamiTF314521

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR003958 CBFA_NFYB_domain
IPR009072 Histone-fold
IPR003956 Transcrpt_fac_NFYB/HAP3_CS
PfamiView protein in Pfam
PF00808 CBFD_NFYB_HMF, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00685 NFYB_HAP3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P25208-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTMDGDSSTT DASQLGISAD YIGGSHYVIQ PHDDTEDSMN DHEDTNGSKE
60 70 80 90 100
SFREQDIYLP IANVARIMKN AIPQTGKIAK DAKECVQECV SEFISFITSE
110 120 130 140 150
ASERCHQEKR KTINGEDILF AMSTLGFDSY VEPLKLYLQK FREAMKGEKG
160 170 180 190 200
IGGAVTATDG LSEELTEEAF TNQLPAGLIT TDGQQQNVMV YTTSYQQISG

VQQIQFS
Length:207
Mass (Da):22,831
Last modified:July 1, 1993 - v2
Checksum:i1ADFA0E45F3CF972
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VSL3F8VSL3_HUMAN
Nuclear transcription factor Y subu...
NFYB
131Annotation score:

Sequence cautioni

The sequence CAA42230 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29I → M in AAH07035 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06145 mRNA Translation: AAA59930.1
X59710 mRNA Translation: CAA42230.1 Different initiation.
AK291934 mRNA Translation: BAF84623.1
CH471054 Genomic DNA Translation: EAW97740.1
BC005316 mRNA Translation: AAH05316.1
BC005317 mRNA Translation: AAH05317.1
BC007035 mRNA Translation: AAH07035.1
CCDSiCCDS9098.1
PIRiS22817
RefSeqiNP_006157.1, NM_006166.3
UniGeneiHs.84928

Genome annotation databases

EnsembliENST00000240055; ENSP00000240055; ENSG00000120837
ENST00000551727; ENSP00000447486; ENSG00000120837
GeneIDi4801
KEGGihsa:4801
UCSCiuc001tkl.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06145 mRNA Translation: AAA59930.1
X59710 mRNA Translation: CAA42230.1 Different initiation.
AK291934 mRNA Translation: BAF84623.1
CH471054 Genomic DNA Translation: EAW97740.1
BC005316 mRNA Translation: AAH05316.1
BC005317 mRNA Translation: AAH05317.1
BC007035 mRNA Translation: AAH07035.1
CCDSiCCDS9098.1
PIRiS22817
RefSeqiNP_006157.1, NM_006166.3
UniGeneiHs.84928

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N1JX-ray1.67A51-143[»]
4AWLX-ray3.08B51-143[»]
4CSRX-ray1.50A51-143[»]
ProteinModelPortaliP25208
SMRiP25208
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110867, 32 interactors
ComplexPortaliCPX-1956 CCAAT-binding factor complex
CORUMiP25208
IntActiP25208, 10 interactors
STRINGi9606.ENSP00000240055

PTM databases

iPTMnetiP25208
PhosphoSitePlusiP25208

Polymorphism and mutation databases

BioMutaiNFYB
DMDMi399193

Proteomic databases

EPDiP25208
MaxQBiP25208
PaxDbiP25208
PeptideAtlasiP25208
PRIDEiP25208
ProteomicsDBi54266

Protocols and materials databases

DNASUi4801
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000240055; ENSP00000240055; ENSG00000120837
ENST00000551727; ENSP00000447486; ENSG00000120837
GeneIDi4801
KEGGihsa:4801
UCSCiuc001tkl.2 human

Organism-specific databases

CTDi4801
DisGeNETi4801
EuPathDBiHostDB:ENSG00000120837.7
GeneCardsiNFYB
HGNCiHGNC:7805 NFYB
HPAiCAB004211
MIMi189904 gene
neXtProtiNX_P25208
OpenTargetsiENSG00000120837
PharmGKBiPA31610
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0869 Eukaryota
COG2036 LUCA
GeneTreeiENSGT00550000074689
HOGENOMiHOG000211832
HOVERGENiHBG008864
InParanoidiP25208
KOiK08065
OMAiKENFREQ
OrthoDBiEOG091G0Y4D
PhylomeDBiP25208
TreeFamiTF314521

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-2426168 Activation of gene expression by SREBF (SREBP)
R-HSA-380994 ATF4 activates genes
R-HSA-381183 ATF6 (ATF6-alpha) activates chaperone genes
SignaLinkiP25208
SIGNORiP25208

Miscellaneous databases

ChiTaRSiNFYB human
EvolutionaryTraceiP25208
GeneWikiiNFYB
GenomeRNAii4801
PROiPR:P25208
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120837 Expressed in 233 organ(s), highest expression level in substantia nigra
CleanExiHS_NFYB
ExpressionAtlasiP25208 baseline and differential
GenevisibleiP25208 HS

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR003958 CBFA_NFYB_domain
IPR009072 Histone-fold
IPR003956 Transcrpt_fac_NFYB/HAP3_CS
PfamiView protein in Pfam
PF00808 CBFD_NFYB_HMF, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00685 NFYB_HAP3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNFYB_HUMAN
AccessioniPrimary (citable) accession number: P25208
Secondary accession number(s): A8K7B9, Q96IY8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 1, 1993
Last modified: November 7, 2018
This is version 188 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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