UniProtKB - P25205 (MCM3_HUMAN)
DNA replication licensing factor MCM3
MCM3
Functioni
Miscellaneous
Catalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 345 – 352 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: ProtInc
- DNA helicase activity Source: UniProtKB-EC
- DNA replication origin binding Source: GO_Central
- single-stranded DNA binding Source: GO_Central
GO - Biological processi
- DNA replication Source: Reactome
- DNA replication initiation Source: Reactome
- DNA strand elongation involved in DNA replication Source: GO_Central
- double-strand break repair via break-induced replication Source: GO_Central
- mitotic DNA replication initiation Source: GO_Central
- pre-replicative complex assembly Source: Reactome
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | Cell cycle, DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P25205 |
Reactomei | R-HSA-176187, Activation of ATR in response to replication stress R-HSA-176974, Unwinding of DNA R-HSA-68867, Assembly of the pre-replicative complex R-HSA-68949, Orc1 removal from chromatin R-HSA-68962, Activation of the pre-replicative complex R-HSA-69052, Switching of origins to a post-replicative state |
SIGNORi | P25205 |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor MCM3 (EC:3.6.4.12)Alternative name(s): DNA polymerase alpha holoenzyme-associated protein P1 P1-MCM3 RLF subunit beta p102 |
Gene namesi | Name:MCM3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6945, MCM3 |
MIMi | 602693, gene |
neXtProti | NX_P25205 |
VEuPathDBi | HostDB:ENSG00000112118.17 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Chromosome By similarity
Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.By similarity
Cytoskeleton
- centrosome Source: HPA
Nucleus
- alpha DNA polymerase:primase complex Source: ProtInc
- CMG complex Source: UniProtKB
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- chromosome, telomeric region Source: BHF-UCL
- MCM complex Source: UniProtKB
- membrane Source: UniProtKB
- perinuclear region of cytoplasm Source: BHF-UCL
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 535 | S → A: 50% reduction in phosphorylation by ATM or ATR. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 4172 |
OpenTargetsi | ENSG00000112118 |
PharmGKBi | PA30691 |
Miscellaneous databases
Pharosi | P25205, Tbio |
Genetic variation databases
BioMutai | MCM3 |
DMDMi | 19857543 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000194093 | 2 – 808 | DNA replication licensing factor MCM3Add BLAST | 807 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | |
Modified residuei | 160 | PhosphoserineCombined sources | 1 | |
Modified residuei | 275 | PhosphoserineCombined sources | 1 | |
Modified residuei | 293 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 535 | Phosphoserine; by ATM1 Publication | 1 | |
Modified residuei | 547 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 611 | PhosphoserineCombined sources | 1 | |
Modified residuei | 668 | PhosphoserineCombined sources | 1 | |
Modified residuei | 672 | PhosphoserineCombined sources | 1 | |
Modified residuei | 674 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 681 | PhosphoserineCombined sources | 1 | |
Modified residuei | 708 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 711 | PhosphoserineCombined sources | 1 | |
Modified residuei | 713 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 722 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 725 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 728 | PhosphoserineBy similarity | 1 | |
Modified residuei | 734 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Glycoprotein, PhosphoproteinProteomic databases
EPDi | P25205 |
jPOSTi | P25205 |
MassIVEi | P25205 |
MaxQBi | P25205 |
PaxDbi | P25205 |
PeptideAtlasi | P25205 |
PRIDEi | P25205 |
ProteomicsDBi | 54265 [P25205-1] |
PTM databases
iPTMneti | P25205 |
MetOSitei | P25205 |
PhosphoSitePlusi | P25205 |
SwissPalmi | P25205 |
Expressioni
Gene expression databases
Bgeei | ENSG00000112118, Expressed in embryo and 239 other tissues |
ExpressionAtlasi | P25205, baseline and differential |
Genevisiblei | P25205, HS |
Organism-specific databases
HPAi | ENSG00000112118, Tissue enhanced (lymphoid) |
Interactioni
Subunit structurei
Component of the MCM2-7 complex (PubMed:16899510, PubMed:17296731). The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (PubMed:16899510, PubMed:17296731). Associated with the replication-specific DNA polymerase alpha (By similarity).
Interacts with MCMBP (PubMed:17296731).
Interacts with ANKRD17 (PubMed:23711367).
Interacts with MCM3AP isoform MCM3AP; this interaction leads to MCM3 acetylation (PubMed:9712829, PubMed:11258703, PubMed:12226073).
Component of the CMG helicase complex, composed of the MCM2-7 complex, the GINS complex and CDC45 (By similarity).
By similarity6 PublicationsBinary interactionsi
Hide detailsP25205
Protein-protein interaction databases
BioGRIDi | 110340, 223 interactors |
ComplexPortali | CPX-2940, MCM complex |
CORUMi | P25205 |
DIPi | DIP-31726N |
IntActi | P25205, 90 interactors |
MINTi | P25205 |
STRINGi | 9606.ENSP00000480987 |
Miscellaneous databases
RNActi | P25205, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 295 – 502 | MCMAdd BLAST | 208 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 477 – 480 | Arginine finger | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0479, Eukaryota |
GeneTreei | ENSGT01010000222249 |
HOGENOMi | CLU_000995_6_0_1 |
InParanoidi | P25205 |
OMAi | LKSVHYC |
OrthoDBi | 266497at2759 |
PhylomeDBi | P25205 |
TreeFami | TF106459 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR031327, MCM IPR008046, Mcm3 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01659, MCMPROTEIN3 |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQSKVRELI SDNQYRLIVN
60 70 80 90 100
VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDAT YAKQYEEFYV
110 120 130 140 150
GLEGSFGSKH VSPRTLTSCF LSCVVCVEGI VTKCSLVRPK VVRSVHYCPA
160 170 180 190 200
TKKTIERRYS DLTTLVAFPS SSVYPTKDEE NNPLETEYGL SVYKDHQTIT
210 220 230 240 250
IQEMPEKAPA GQLPRSVDVI LDDDLVDKAK PGDRVQVVGT YRCLPGKKGG
260 270 280 290 300
YTSGTFRTVL IACNVKQMSK DAQPSFSAED IAKIKKFSKT RSKDIFDQLA
310 320 330 340 350
KSLAPSIHGH DYVKKAILCL LLGGVERDLE NGSHIRGDIN ILLIGDPSVA
360 370 380 390 400
KSQLLRYVLC TAPRAIPTTG RGSSGVGLTA AVTTDQETGE RRLEAGAMVL
410 420 430 440 450
ADRGVVCIDE FDKMSDMDRT AIHEVMEQGR VTIAKAGIHA RLNARCSVLA
460 470 480 490 500
AANPVYGRYD QYKTPMENIG LQDSLLSRFD LLFIMLDQMD PEQDREISDH
510 520 530 540 550
VLRMHRYRAP GEQDGDAMPL GSAVDILATD DPNFSQEDQQ DTQIYEKHDN
560 570 580 590 600
LLHGTKKKKE KMVSAAFMKK YIHVAKIIKP VLTQESATYI AEEYSRLRSQ
610 620 630 640 650
DSMSSDTART SPVTARTLET LIRLATAHAK ARMSKTVDLQ DAEEAVELVQ
660 670 680 690 700
YAYFKKVLEK EKKRKKRSED ESETEDEEEK SQEDQEQKRK RRKTRQPDAK
710 720 730 740 750
DGDSYDPYDF SDTEEEMPQV HTPKTADSQE TKESQKVELS ESRLKAFKVA
760 770 780 790 800
LLDVFREAHA QSIGMNRLTE SINRDSEEPF SSVEIQAALS KMQDDNQVMV
SEGIIFLI
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3KQ69 | J3KQ69_HUMAN | DNA helicase | MCM3 | 762 | Annotation score: | ||
A0A499FHX9 | A0A499FHX9_HUMAN | DNA helicase | MCM3 | 818 | Annotation score: | ||
Q7Z6P5 | Q7Z6P5_HUMAN | DNA replication licensing factor MC... | MCM3 | 328 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 230 – 231 | KP → NA in BAA07267 (PubMed:7758114).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_014810 | 105 | S → G. Corresponds to variant dbSNP:rs2307332Ensembl. | 1 | |
Natural variantiVAR_014811 | 280 | D → V1 PublicationCorresponds to variant dbSNP:rs2307329Ensembl. | 1 | |
Natural variantiVAR_014812 | 287 | F → L1 PublicationCorresponds to variant dbSNP:rs2307328Ensembl. | 1 | |
Natural variantiVAR_020516 | 590 | I → L1 PublicationCorresponds to variant dbSNP:rs17240063Ensembl. | 1 | |
Natural variantiVAR_020517 | 774 | R → W1 PublicationCorresponds to variant dbSNP:rs2230239Ensembl. | 1 | |
Natural variantiVAR_020427 | 777 | E → K1 PublicationCorresponds to variant dbSNP:rs2230240Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_057050 | 1 | M → MLPRSPPLPRGNLWWREEFG SFRAGVESSWEPPRDFGGGS SLAAGM in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62153 mRNA Translation: CAA44078.2 D38073 mRNA Translation: BAA07267.1 AK301704 mRNA Translation: BAG63176.1 AY621074 Genomic DNA Translation: AAT27321.1 AL034343 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX04367.1 BC001626 mRNA Translation: AAH01626.1 BC003509 mRNA Translation: AAH03509.2 |
CCDSi | CCDS4940.2 [P25205-1] |
PIRi | S62594 |
RefSeqi | NP_001257401.1, NM_001270472.1 NP_002379.3, NM_002388.4 [P25205-1] |
Genome annotation databases
Ensembli | ENST00000596288; ENSP00000472940; ENSG00000112118 [P25205-1] ENST00000616552; ENSP00000480987; ENSG00000112118 [P25205-2] |
GeneIDi | 4172 |
KEGGi | hsa:4172 |
UCSCi | uc003pan.2, human [P25205-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62153 mRNA Translation: CAA44078.2 D38073 mRNA Translation: BAA07267.1 AK301704 mRNA Translation: BAG63176.1 AY621074 Genomic DNA Translation: AAT27321.1 AL034343 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX04367.1 BC001626 mRNA Translation: AAH01626.1 BC003509 mRNA Translation: AAH03509.2 |
CCDSi | CCDS4940.2 [P25205-1] |
PIRi | S62594 |
RefSeqi | NP_001257401.1, NM_001270472.1 NP_002379.3, NM_002388.4 [P25205-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6XTX | electron microscopy | 3.29 | 3 | 1-808 | [»] | |
6XTY | electron microscopy | 6.77 | 3 | 1-808 | [»] | |
SMRi | P25205 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110340, 223 interactors |
ComplexPortali | CPX-2940, MCM complex |
CORUMi | P25205 |
DIPi | DIP-31726N |
IntActi | P25205, 90 interactors |
MINTi | P25205 |
STRINGi | 9606.ENSP00000480987 |
PTM databases
iPTMneti | P25205 |
MetOSitei | P25205 |
PhosphoSitePlusi | P25205 |
SwissPalmi | P25205 |
Genetic variation databases
BioMutai | MCM3 |
DMDMi | 19857543 |
Proteomic databases
EPDi | P25205 |
jPOSTi | P25205 |
MassIVEi | P25205 |
MaxQBi | P25205 |
PaxDbi | P25205 |
PeptideAtlasi | P25205 |
PRIDEi | P25205 |
ProteomicsDBi | 54265 [P25205-1] |
Protocols and materials databases
Antibodypediai | 1433, 499 antibodies |
DNASUi | 4172 |
Genome annotation databases
Ensembli | ENST00000596288; ENSP00000472940; ENSG00000112118 [P25205-1] ENST00000616552; ENSP00000480987; ENSG00000112118 [P25205-2] |
GeneIDi | 4172 |
KEGGi | hsa:4172 |
UCSCi | uc003pan.2, human [P25205-1] |
Organism-specific databases
CTDi | 4172 |
DisGeNETi | 4172 |
GeneCardsi | MCM3 |
HGNCi | HGNC:6945, MCM3 |
HPAi | ENSG00000112118, Tissue enhanced (lymphoid) |
MIMi | 602693, gene |
neXtProti | NX_P25205 |
OpenTargetsi | ENSG00000112118 |
PharmGKBi | PA30691 |
VEuPathDBi | HostDB:ENSG00000112118.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0479, Eukaryota |
GeneTreei | ENSGT01010000222249 |
HOGENOMi | CLU_000995_6_0_1 |
InParanoidi | P25205 |
OMAi | LKSVHYC |
OrthoDBi | 266497at2759 |
PhylomeDBi | P25205 |
TreeFami | TF106459 |
Enzyme and pathway databases
PathwayCommonsi | P25205 |
Reactomei | R-HSA-176187, Activation of ATR in response to replication stress R-HSA-176974, Unwinding of DNA R-HSA-68867, Assembly of the pre-replicative complex R-HSA-68949, Orc1 removal from chromatin R-HSA-68962, Activation of the pre-replicative complex R-HSA-69052, Switching of origins to a post-replicative state |
SIGNORi | P25205 |
Miscellaneous databases
BioGRID-ORCSi | 4172, 710 hits in 977 CRISPR screens |
ChiTaRSi | MCM3, human |
GeneWikii | MCM3 |
GenomeRNAii | 4172 |
Pharosi | P25205, Tbio |
PROi | PR:P25205 |
RNActi | P25205, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000112118, Expressed in embryo and 239 other tissues |
ExpressionAtlasi | P25205, baseline and differential |
Genevisiblei | P25205, HS |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR031327, MCM IPR008046, Mcm3 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01659, MCMPROTEIN3 |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCM3_HUMAN | |
Accessioni | P25205Primary (citable) accession number: P25205 Secondary accession number(s): B4DWW4 Q9NUE7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | August 14, 2001 | |
Last modified: | April 7, 2021 | |
This is version 234 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families