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Protein

Probable 26S proteasome non-ATPase regulatory subunit 3

Gene

Rpn3

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.By similarity

Caution

Was originally thought to be the diphenol oxidase A2 component involved in catecholamine metabolism, melanin formation, and sclerotization of the cuticle.1 Publication

GO - Molecular functioni

  • enzyme regulator activity Source: InterPro
  • zinc ion binding Source: FlyBase

GO - Biological processi

Enzyme and pathway databases

BRENDAi3.4.25.1 1994
ReactomeiR-DME-1169091 Activation of NF-kappaB in B cells
R-DME-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-DME-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-DME-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-DME-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-DME-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-DME-187577 SCF(Skp2)-mediated degradation of p27/p21
R-DME-195253 Degradation of beta-catenin by the destruction complex
R-DME-202424 Downstream TCR signaling
R-DME-209360 Ubiquitination and proteolysis of phosphorylated CI
R-DME-209406 Degradation of NF-kappa-B inhibitor, CACT
R-DME-209461 Ubiquitination and degradation of phosphorylated ARM
R-DME-216167 Nuclear CI is degraded
R-DME-2871837 FCERI mediated NF-kB activation
R-DME-432395 Degradation of TIM
R-DME-432524 Degradation of PER
R-DME-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-DME-4608870 Asymmetric localization of PCP proteins
R-DME-4641257 Degradation of AXIN
R-DME-4641258 Degradation of DVL
R-DME-5358346 Hedgehog ligand biogenesis
R-DME-538848 Degradation of CLK
R-DME-538864 Degradation of CRY
R-DME-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-DME-5607764 CLEC7A (Dectin-1) signaling
R-DME-5610785 GLI3 is processed to GLI3R by the proteasome
R-DME-5632684 Hedgehog 'on' state
R-DME-5658442 Regulation of RAS by GAPs
R-DME-5676590 NIK-->noncanonical NF-kB signaling
R-DME-5689603 UCH proteinases
R-DME-5689880 Ub-specific processing proteases
R-DME-6798695 Neutrophil degranulation
R-DME-68949 Orc1 removal from chromatin
R-DME-69017 CDK-mediated phosphorylation and removal of Cdc6
R-DME-69229 Ubiquitin-dependent degradation of Cyclin D1
R-DME-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-DME-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-DME-8939902 Regulation of RUNX2 expression and activity
R-DME-8941858 Regulation of RUNX3 expression and activity
R-DME-8948751 Regulation of PTEN stability and activity
R-DME-9020702 Interleukin-1 signaling
R-DME-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 26S proteasome non-ATPase regulatory subunit 3
Short name:
26S proteasome subunit S3
Alternative name(s):
Diphenol oxidase A2 component
Short name:
DOX-A2
Regulatory particle non-ATPase 3
Gene namesi
Name:Rpn3
Synonyms:Dox-A2
ORF Names:CG42641
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0261396 Rpn3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738211 – 494Probable 26S proteasome non-ATPase regulatory subunit 3Add BLAST494

Proteomic databases

PaxDbiP25161
PRIDEiP25161

Expressioni

Tissue specificityi

Blood (crystal) cells and cuticle.

Gene expression databases

BgeeiFBgn0261396
ExpressionAtlasiP25161 baseline and differential
GenevisibleiP25161 DM

Interactioni

Subunit structurei

The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively (By similarity).By similarity

Protein-protein interaction databases

BioGridi61163, 21 interactors
DIPiDIP-18295N
IntActiP25161, 6 interactors
STRINGi7227.FBpp0291495

Structurei

3D structure databases

ProteinModelPortaliP25161
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini247 – 426PCIPROSITE-ProRule annotationAdd BLAST180

Sequence similaritiesi

Belongs to the proteasome subunit S3 family.Curated

Phylogenomic databases

eggNOGiKOG2581 Eukaryota
ENOG410XS40 LUCA
GeneTreeiENSGT00490000043406
InParanoidiP25161
KOiK03033
OMAiAMRYPPK
OrthoDBiEOG091G05QN
PhylomeDBiP25161

Family and domain databases

InterProiView protein in InterPro
IPR013586 26S_Psome_reg_C
IPR000717 PCI_dom
IPR035267 PSMD3
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10758:SF2 PTHR10758:SF2, 1 hit
PfamiView protein in Pfam
PF01399 PCI, 1 hit
PF08375 Rpn3_C, 1 hit
SMARTiView protein in SMART
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequencei

Sequence statusi: Complete.

P25161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNATDIGAN DVEMEVDPTA ETLADEKKNQ DVAAVQEIRE QIRQIEKGVA
60 70 80 90 100
SKESRFILRV LRNLPNTRRK LNGVVFRNLA QSIYPAGADR EAAVALMPAV
110 120 130 140 150
EKDATELPDV PKKQVATKAP IAEVDAYFYL LLLVKLIDAS DLKRAGISAD
160 170 180 190 200
ALMAKISIQN RRTLDLIGAK SYFYFSRVAE LKNSLEGIRS FLHARLRTAT
210 220 230 240 250
LRNDFEGQAV LINCLLRNYL HYALYDQADK LVKKSVYPES ASNNEWARFL
260 270 280 290 300
YYLGRIKAAK LEYSDAHKHL VQALRKSPQH AAIGFRQTVQ KLIIVVELLL
310 320 330 340 350
GNIPERVVFR QAGLRQSLGA YFQLTQAVRL GNLKRFGDVV SQYGPKFQLD
360 370 380 390 400
HTFTLIIRLR HNVIKTAIRS IGLSYSRISP QDIAKRLMLD SAEDAEFIVS
410 420 430 440 450
KAIRDGVIEA TLDPAQNFMR SKESTDIYST REPQLAFHER ISFCLNLHNQ
460 470 480 490
SVKAMRYPPK SYGKDLESAE ERREREQQDL ELAKEMAEDD EDGF
Length:494
Mass (Da):56,004
Last modified:May 1, 1992 - v1
Checksum:i8659CBCAFF95B735
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti204D → H in AAL90075 (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63010 Genomic DNA Translation: AAB00732.1
AE014134 Genomic DNA Translation: AAF53749.1
AY089337 mRNA Translation: AAL90075.1
BT044249 mRNA Translation: ACH92314.1
PIRiJH0665
RefSeqiNP_001260557.1, NM_001273628.1
NP_477300.1, NM_057952.4
UniGeneiDm.4848

Genome annotation databases

EnsemblMetazoaiFBtr0302289; FBpp0291495; FBgn0261396
FBtr0336843; FBpp0307804; FBgn0261396
GeneIDi35176
KEGGidme:Dmel_CG42641

Similar proteinsi

Entry informationi

Entry nameiPSMD3_DROME
AccessioniPrimary (citable) accession number: P25161
Secondary accession number(s): B5RIX5, Q8T460, Q9VJ09
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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