Repressor ROX1

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• cis-regulatory region sequence-specific DNA binding Source: SGD <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "Molecular mechanism of the multiple regulation of the Saccharomyces cerevisiae ATF1 gene encoding alcohol acetyltransferase."
    Fujiwara D., Kobayashi O., Yoshimoto H., Harashima S., Tamai Y.
    Yeast 15:1183-1197(1999) [PubMed] [Europe PMC] [Abstract]
• DNA binding, bending Source: SGDInferred from direct assayⁱ
  • "Characterization of the DNA binding and bending HMG domain of the yeast hypoxic repressor Rox1."
    Deckert J., Khalaf R.A., Hwang S.M., Zitomer R.S.
    Nucleic Acids Res 27:3518-3526(1999) [PubMed] [Europe PMC] [Abstract]
  • "Mutational analysis of Rox1, a DNA-bending repressor of hypoxic genes in Saccharomyces cerevisiae."
    Deckert J., Rodriguez Torres A.M., Simon J.T., Zitomer R.S.
    Mol Cell Biol 15:6109-6117(1995) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription repressor activity, RNA polymerase II-specific Source: SGD <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Characterization of the DNA binding and bending HMG domain of the yeast hypoxic repressor Rox1."
    Deckert J., Khalaf R.A., Hwang S.M., Zitomer R.S.
    Nucleic Acids Res 27:3518-3526(1999) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_CentralInferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• sequence-specific DNA binding Source: SGDInferred from direct assayⁱ
  • "Characterization of the DNA binding and bending HMG domain of the yeast hypoxic repressor Rox1."
    Deckert J., Khalaf R.A., Hwang S.M., Zitomer R.S.
    Nucleic Acids Res 27:3518-3526(1999) [PubMed] [Europe PMC] [Abstract]
  • Ref.1

    "The Rox1 repressor of the Saccharomyces cerevisiae hypoxic genes is a specific DNA-binding protein with a high-mobility-group motif."
    Balasubramanian B., Lowry C.V., Zitomer R.S.
    Mol. Cell. Biol. 13:6071-6078(1993) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
• transcription corepressor activity Source: SGDInferred from mutant phenotypeⁱ
  • "Recruitment of Tup1-Ssn6 by yeast hypoxic genes and chromatin-independent exclusion of TATA binding protein."
    Mennella T.A., Klinkenberg L.G., Zitomer R.S.
    Eukaryot. Cell 2:1288-1303(2003) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• cellular response to osmotic stress Source: SGDInferred from mutant phenotypeⁱ
  • "Activator and repressor functions of the Mot3 transcription factor in the osmostress response of Saccharomyces cerevisiae."
    Martinez-Montanes F., Rienzo A., Poveda-Huertes D., Pascual-Ahuir A., Proft M.
    Eukaryot Cell 12:636-647(2013) [PubMed] [Europe PMC] [Abstract]
• negative regulation of transcription by RNA polymerase II Source: SGDInferred from mutant phenotypeⁱ
  • "Characterization of the DNA binding and bending HMG domain of the yeast hypoxic repressor Rox1."
    Deckert J., Khalaf R.A., Hwang S.M., Zitomer R.S.
    Nucleic Acids Res 27:3518-3526(1999) [PubMed] [Europe PMC] [Abstract]
  • "Molecular mechanism of the multiple regulation of the Saccharomyces cerevisiae ATF1 gene encoding alcohol acetyltransferase."
    Fujiwara D., Kobayashi O., Yoshimoto H., Harashima S., Tamai Y.
    Yeast 15:1183-1197(1999) [PubMed] [Europe PMC] [Abstract]
  • "Regulation of Saccharomyces cerevisiae FET4 by oxygen and iron."
    Jensen L.T., Culotta V.C.
    J Mol Biol 318:251-260(2002) [PubMed] [Europe PMC] [Abstract]
  • "Combinatorial control of yeast FET4 gene expression by iron, zinc, and oxygen."
    Waters B.M., Eide D.J.
    J Biol Chem 277:33749-33757(2002) [PubMed] [Europe PMC] [Abstract]
  • "HAP1 and ROX1 form a regulatory pathway in the repression of HEM13 transcription in Saccharomyces cerevisiae."
    Keng T.
    Mol Cell Biol 12:2616-2623(1992) [PubMed] [Europe PMC] [Abstract]
  • "Synergy among differentially regulated repressors of the ribonucleotide diphosphate reductase genes of Saccharomyces cerevisiae."
    Klinkenberg L.G., Webb T., Zitomer R.S.
    Eukaryot Cell 5:1007-1017(2006) [PubMed] [Europe PMC] [Abstract]
  • "Inverse regulation of the yeast COX5 genes by oxygen and heme."
    Hodge M.R., Kim G., Singh K., Cumsky M.G.
    Mol. Cell. Biol. 9:1958-1964(1989) [PubMed] [Europe PMC] [Abstract]
  • "Differential regulation of the two genes encoding Saccharomyces cerevisiae cytochrome c oxidase subunit V by heme and the HAP2 and REO1 genes."
    Trueblood C.E., Wright R.M., Poyton R.O.
    Mol Cell Biol 8:4537-4540(1988) [PubMed] [Europe PMC] [Abstract]
  • "ROX1 encodes a heme-induced repression factor regulating ANB1 and CYC7 of Saccharomyces cerevisiae."
    Lowry C.V., Zitomer R.S.
    Mol. Cell. Biol. 8:4651-4658(1988) [PubMed] [Europe PMC] [Abstract]
  • "REO1 and ROX1 are alleles of the same gene which encodes a transcriptional repressor of hypoxic genes in Saccharomyces cerevisiae."
    Kwast K.E., Burke P.V., Brown K., Poyton R.O.
    Curr Genet 32:377-383(1997) [PubMed] [Europe PMC] [Abstract]
• negative regulation of transcription from RNA polymerase II promoter in response to stress Source: SGDInferred from mutant phenotypeⁱ
  • "Repression of ergosterol biosynthesis is essential for stress resistance and is mediated by the Hog1 MAP kinase and the Mot3 and Rox1 transcription factors."
    Montanes F.M., Pascual-Ahuir A., Proft M.
    Mol Microbiol 79:1008-1023(2011) [PubMed] [Europe PMC] [Abstract]
• regulation of transcription by RNA polymerase II Source: GO_CentralInferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

• UniProt annotation
• GO - Cellular component

Keywords - Cellular componentⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductionⁱ

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Compositional bias

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MNPKSSTPKI PRPKNAFILF RQHYHRILID EWTAQGVEIP HNSNISKIIG 
        60         70         80         90        100
TKWKGLQPED KAHWENLAEK EKLEHERKYP EYKYKPVRKS KKKQLLLKEI 
       110        120        130        140        150
EQQQQQQQKE QQQQKQSQPQ LQQPFNNNIV LMKRAHSLSP SSSVSSSNSY 
       160        170        180        190        200
QFQLNNDLKR LPIPSVNTSN YMVSRSLSGL PLTHDKTARD LPQLSSQLNS 
       210        220        230        240        250
IPYYSAPHDP STRHHYLNVA QAQPRANSTP QLPFISSIIN NSSQTPVTTT 
       260        270        280        290        300
TTSTTTATSS PGKFSSSPNS SVLENNRLNS INNSNQYLPP PLLPSLQDFQ 
       310        320        330        340        350
LDQYQQLKQM GPTYIVKPLS HTRNNLLSTT TPTHHHIPHI PNQNIPLHQI 
       360 
INSSNTEVTA KTSLVSPK                                    

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. Yeast
   Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
2. Yeast chromosome XVI
   Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names