UniProtKB - P25023 (BKRB2_RAT)
Protein
B2 bradykinin receptor
Gene
Bdkrb2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
GO - Molecular functioni
- beta-2 adrenergic receptor binding Source: RGD
- bradykinin receptor activity Source: RGD
- G protein-coupled receptor activity Source: GO_Central
- protease binding Source: RGD
- protein heterodimerization activity Source: RGD
- type 1 angiotensin receptor binding Source: RGD
GO - Biological processi
- acute inflammatory response to antigenic stimulus Source: RGD
- arachidonic acid secretion Source: RGD
- cellular response to hypoxia Source: RGD
- G protein-coupled receptor signaling pathway Source: GO_Central
- maintenance of blood-brain barrier Source: RGD
- negative regulation of blood pressure Source: RGD
- negative regulation of cell population proliferation Source: RGD
- negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress Source: RGD
- negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator Source: RGD
- negative regulation of peptidyl-serine phosphorylation Source: RGD
- positive regulation of cytosolic calcium ion concentration Source: RGD
- response to drug Source: RGD
- response to salt stress Source: RGD
- sensory perception of pain Source: RGD
- smooth muscle contraction Source: InterPro
- vasoconstriction Source: InterPro
- vasodilation Source: RGD
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-416476, G alpha (q) signalling events R-RNO-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: B2 bradykinin receptorShort name: B2R Short name: BK-2 receptor |
Gene namesi | Name:Bdkrb2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2201, Bdkrb2 |
Subcellular locationi
Plasma membrane
Endosome
- endosome Source: RGD
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 65 | ExtracellularSequence analysisAdd BLAST | 65 | |
Transmembranei | 66 – 89 | Helical; Name=1Sequence analysisAdd BLAST | 24 | |
Topological domaini | 90 – 98 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 99 – 123 | Helical; Name=2Sequence analysisAdd BLAST | 25 | |
Topological domaini | 124 – 136 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 137 – 158 | Helical; Name=3Sequence analysisAdd BLAST | 22 | |
Topological domaini | 159 – 180 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 181 – 203 | Helical; Name=4Sequence analysisAdd BLAST | 23 | |
Topological domaini | 204 – 226 | ExtracellularSequence analysisAdd BLAST | 23 | |
Transmembranei | 227 – 253 | Helical; Name=5Sequence analysisAdd BLAST | 27 | |
Topological domaini | 254 – 272 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 273 – 297 | Helical; Name=6Sequence analysisAdd BLAST | 25 | |
Topological domaini | 298 – 316 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 317 – 340 | Helical; Name=7Sequence analysisAdd BLAST | 24 | |
Topological domaini | 341 – 396 | CytoplasmicSequence analysisAdd BLAST | 56 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069194 | 1 – 396 | B2 bradykinin receptorAdd BLAST | 396 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 33 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 44 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 135 ↔ 216 | PROSITE-ProRule annotation | ||
Modified residuei | 161 | Phosphotyrosine1 Publication | 1 | |
Glycosylationi | 212 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 352 | Phosphotyrosine1 Publication | 1 | |
Lipidationi | 356 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 365 | Phosphoserine1 Publication | 1 | |
Modified residuei | 371 | Phosphoserine1 Publication | 1 | |
Modified residuei | 374 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 378 | Phosphoserine; by GRK61 Publication | 1 | |
Modified residuei | 380 | Phosphoserine; by GRK61 Publication | 1 |
Post-translational modificationi
Diphosphorylation at Ser-365 and Ser-371, at Ser-378 and Ser-380, and at Thr-374 and Ser-380 seem to be correlated pairwise.
Palmitoylation at Cys-356 and phosphorylation at Tyr-352 seem to be mutually exclusive.1 Publication
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | P25023 |
PRIDEi | P25023 |
PTM databases
GlyGeni | P25023, 3 sites |
iPTMneti | P25023 |
PhosphoSitePlusi | P25023 |
SwissPalmi | P25023 |
Expressioni
Tissue specificityi
Uterus, vas deferens, kidney, ileum, heart, testis, lung and brain.
Gene expression databases
Genevisiblei | P25023, RN |
Interactioni
Subunit structurei
Forms a complex with PECAM1 and GNAQ.
Interacts with PECAM1 (By similarity).
By similarityGO - Molecular functioni
- beta-2 adrenergic receptor binding Source: RGD
- protease binding Source: RGD
- protein heterodimerization activity Source: RGD
- type 1 angiotensin receptor binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000064990 |
Chemistry databases
BindingDBi | P25023 |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QTX6, Eukaryota |
InParanoidi | P25023 |
OrthoDBi | 837077at2759 |
PhylomeDBi | P25023 |
Family and domain databases
InterProi | View protein in InterPro IPR001504, Brdyknn_2_rcpt IPR000496, Brdyknn_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00425, BRADYKININR PR00994, BRADYKINNB2R PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform Long (identifier: P25023-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDTRSSLCPK TQAVVAVFWG PGCHLSTCIE MFNITTQALG SAHNGTFSEV
60 70 80 90 100
NCPDTEWWSW LNAIQAPFLW VLFLLAALEN IFVLSVFCLH KTNCTVAEIY
110 120 130 140 150
LGNLAAADLI LACGLPFWAI TIANNFDWLF GEVLCRVVNT MIYMNLYSSI
160 170 180 190 200
CFLMLVSIDR YLALVKTMSM GRMRGVRWAK LYSLVIWSCT LLLSSPMLVF
210 220 230 240 250
RTMKDYREEG HNVTACVIVY PSRSWEVFTN MLLNLVGFLL PLSIITFCTV
260 270 280 290 300
RIMQVLRNNE MKKFKEVQTE KKATVLVLAV LGLFVLCWFP FQISTFLDTL
310 320 330 340 350
LRLGVLSGCW NERAVDIVTQ ISSYVAYSNS CLNPLVYVIV GKRFRKKSRE
360 370 380 390
VYQAICRKGG CMGESVQMEN SMGTLRTSIS VDRQIHKLQD WAGNKQ
Isoform Short (identifier: P25023-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-30: Missing.
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V942 | G3V942_RAT | B2 bradykinin receptor | Bdkrb2 rCG_27741 | 398 | Annotation score: | ||
A0A0H2UI29 | A0A0H2UI29_RAT | B2 bradykinin receptor | Bdkrb2 rCG_27741 | 392 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 106 | A → G (PubMed:7929432).Curated | 1 | |
Sequence conflicti | 106 | A → G (PubMed:8086459).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001867 | 1 – 30 | Missing in isoform Short. 1 PublicationAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59967 mRNA Translation: AAA16425.1 M59967 mRNA Translation: AAA16426.1 X80187 Genomic DNA No translation available. X80188 Genomic DNA No translation available. X80189 Genomic DNA No translation available. X80190 Genomic DNA No translation available. L26173 Genomic DNA Translation: AAA62492.1 X69681 mRNA Translation: CAA49361.1 |
PIRi | A41283, OORTB2 |
RefSeqi | NP_775123.2, NM_173100.2 |
Genome annotation databases
GeneIDi | 25245 |
KEGGi | rno:25245 |
UCSCi | RGD:2201, rat [P25023-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59967 mRNA Translation: AAA16425.1 M59967 mRNA Translation: AAA16426.1 X80187 Genomic DNA No translation available. X80188 Genomic DNA No translation available. X80189 Genomic DNA No translation available. X80190 Genomic DNA No translation available. L26173 Genomic DNA Translation: AAA62492.1 X69681 mRNA Translation: CAA49361.1 |
PIRi | A41283, OORTB2 |
RefSeqi | NP_775123.2, NM_173100.2 |
3D structure databases
SMRi | P25023 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000064990 |
Chemistry databases
BindingDBi | P25023 |
ChEMBLi | CHEMBL2501 |
DrugCentrali | P25023 |
GuidetoPHARMACOLOGYi | 42 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P25023, 3 sites |
iPTMneti | P25023 |
PhosphoSitePlusi | P25023 |
SwissPalmi | P25023 |
Proteomic databases
PaxDbi | P25023 |
PRIDEi | P25023 |
Genome annotation databases
GeneIDi | 25245 |
KEGGi | rno:25245 |
UCSCi | RGD:2201, rat [P25023-1] |
Organism-specific databases
CTDi | 624 |
RGDi | 2201, Bdkrb2 |
Phylogenomic databases
eggNOGi | ENOG502QTX6, Eukaryota |
InParanoidi | P25023 |
OrthoDBi | 837077at2759 |
PhylomeDBi | P25023 |
Enzyme and pathway databases
Reactomei | R-RNO-416476, G alpha (q) signalling events R-RNO-418594, G alpha (i) signalling events |
Miscellaneous databases
PROi | PR:P25023 |
Gene expression databases
Genevisiblei | P25023, RN |
Family and domain databases
InterProi | View protein in InterPro IPR001504, Brdyknn_2_rcpt IPR000496, Brdyknn_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00425, BRADYKININR PR00994, BRADYKINNB2R PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BKRB2_RAT | |
Accessioni | P25023Primary (citable) accession number: P25023 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | December 15, 1998 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries