UniProtKB - P24937 (CAP6_ADE05)
Pre-protein VI
L3
Functioni
During virus assembly, promotes hexon trimers nuclear import through nuclear pore complexes via an importin alpha/beta-dependent mechanism. By analogy to herpesviruses capsid assembly, might act as a chaperone to promote the formation of the icosahedral capsid.
UniRule annotation1 PublicationStructural component of the virion that provides increased stability to the particle shell through its interaction with the core-capsid bridging protein and the hexon-linking protein VIII (PubMed:25071205).
Fibers shedding during virus entry into host cell allows the endosome lysis protein to be exposed as a membrane-lytic peptide (By similarity).
Exhibits pH-independent membrane fragmentation activity and probably mediates viral rapid escape from host endosome via organellar membrane lysis (PubMed:15681401, PubMed:21209115, PubMed:20409568, PubMed:22516138).
It is not clear if it then remains partially associated with the capsid and involved in the intracellular microtubule-dependent transport of capsid to the nucleus, or if it is lost during endosomal penetration (PubMed:20333243).
UniRule annotationBy similarity6 PublicationsCofactor that activates the viral protease. Binds to viral protease in a 1:1 ratio.
UniRule annotationMiscellaneous
GO - Biological processi
- lysis of host organelle involved in viral entry into host cell Source: UniProtKB
- microtubule-dependent intracellular transport of viral material towards nucleus Source: UniProtKB-UniRule
- viral release from host cell Source: UniProtKB-UniRule
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Pre-protein VIUniRule annotationShort name: pVIUniRule annotation Cleaved into the following 2 chains: Endosome lysis proteinUniRule annotation Protease cofactorUniRule annotation Alternative name(s): pVI-CUniRule annotation |
Gene namesi | Name:L3UniRule annotation |
Organismi | Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5) |
Taxonomic identifieri | 28285 [NCBI] |
Taxonomic lineagei | Viruses › Varidnaviria › Bamfordvirae › Preplasmiviricota › Tectiliviricetes › Rowavirales › Adenoviridae › Mastadenovirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Host nucleus UniRule annotation1 Publication
- Host cytoplasm UniRule annotation1 Publication Note: Shuttles between host cytoplasm and nucleus.UniRule annotation1 Publication
- Virion UniRule annotation1 Publication Note: Associates with the base of each peripentonal hexon on the capsid interior. Present in around 360 copies per virion.UniRule annotation1 Publication
Keywords - Cellular componenti
Capsid protein, Host cytoplasm, Host nucleus, VirionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 40 | L → Q: Impaired endosome penetration and reduces infectivity. 1 Publication | 1 | |
Mutagenesisi | 48 | G → C: Decreased infectivity and endosomal membrane disruption activity. 1 Publication | 1 | |
Mutagenesisi | 149 – 151 | PSY → GAA: No effect on endosomal escape; defective in microtubule-dependent trafficking toward the nucleus. 1 Publication | 3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000421133 | 1 – 250 | Pre-protein VIUniRule annotationAdd BLAST | 250 | |
PropeptideiPRO_0000036546 | 1 – 33 | UniRule annotationAdd BLAST | 33 | |
ChainiPRO_0000036547 | 34 – 239 | Endosome lysis proteinUniRule annotationAdd BLAST | 206 | |
PeptideiPRO_0000036548 | 240 – 250 | Protease cofactorAdd BLAST | 11 | |
ChainiPRO_0000439553 | 240 – 250 | Protease cofactorUniRule annotationAdd BLAST | 11 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 124 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 143 | Phosphothreonine; by hostUniRule annotation | 1 | |
Disulfide bondi | 249 | Interchain (with Adenovirus protease)UniRule annotation |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 33 – 34 | Cleavage; by viral proteaseUniRule annotation | 2 | |
Sitei | 239 – 240 | Cleavage; by viral proteaseUniRule annotation | 2 |
Keywords - PTMi
Disulfide bond, Phosphoprotein, Ubl conjugationExpressioni
Inductioni
Keywords - Developmental stagei
Late proteinInteractioni
Subunit structurei
Interacts (via PPxY motif) with host NEDD4 ubiquitine ligase; this interaction might play a role in virus intracellular transport during entry (PubMed:20333243).
Part of a complex composed of the core-capsid bridging protein, the endosome lysis protein VI and the hexon-linking protein VIII; these interactions bridge the virus core to the capsid (PubMed:25071205).
Interacts with peripentonal hexons; this interaction stabilizes the capsid by gluing two peripentonal hexons together and joining them with an adjacent group-of-nine hexon (PubMed:25071205).
UniRule annotation2 PublicationsHeterodimer with the viral protease; disulfide-linked.
Interacts with the viral protease.
UniRule annotationStructurei
3D structure databases
SMRi | P24937 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24937 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 54 | Amphipathic alpha-helix essential for membrane lytic activityUniRule annotationAdd BLAST | 21 | |
Regioni | 36 – 53 | Involved in endosomal membrane lysisUniRule annotationAdd BLAST | 18 | |
Regioni | 48 – 74 | Interaction with hexon proteinUniRule annotationAdd BLAST | 27 | |
Regioni | 103 – 148 | DisorderedSequence analysisAdd BLAST | 46 | |
Regioni | 233 – 239 | Interaction with hexon proteinUniRule annotation | 7 | |
Regioni | 240 – 250 | Binds to importin alpha/beta, involved in hexon nuclear importUniRule annotationAdd BLAST | 11 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 67 – 76 | Nuclear export signalUniRule annotation | 10 | |
Motifi | 131 – 135 | Nuclear localization signalUniRule annotation | 5 | |
Motifi | 148 – 151 | PPXY motifUniRule annotation | 4 | |
Motifi | 231 – 242 | Nuclear export signalUniRule annotationAdd BLAST | 12 | |
Motifi | 245 – 248 | Nuclear localization signalUniRule annotation | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 125 – 143 | Basic and acidic residuesSequence analysisAdd BLAST | 19 |
Domaini
Sequence similaritiesi
Family and domain databases
DisProti | DP00808 |
HAMAPi | MF_04048, ADV_CAP6, 1 hit |
InterProi | View protein in InterPro IPR004243, McpVI |
Pfami | View protein in Pfam PF02993, MCPVI, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MEDINFASLA PRHGSRPFMG NWQDIGTSNM SGGAFSWGSL WSGIKNFGST
60 70 80 90 100
VKNYGSKAWN SSTGQMLRDK LKEQNFQQKV VDGLASGISG VVDLANQAVQ
110 120 130 140 150
NKINSKLDPR PPVEEPPPAV ETVSPEGRGE KRPRPDREET LVTQIDEPPS
160 170 180 190 200
YEEALKQGLP TTRPIAPMAT GVLGQHTPVT LDLPPPADTQ QKPVLPGPTA
210 220 230 240 250
VVVTRPSRAS LRRAASGPRS LRPVASGNWQ STLNSIVGLG VQSLKRRRCF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M73260 Genomic DNA Translation: AAA96411.1 |
PIRi | D39449, Q5ADB5 |
RefSeqi | AP_000210.1, AC_000008.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M73260 Genomic DNA Translation: AAA96411.1 |
PIRi | D39449, Q5ADB5 |
RefSeqi | AP_000210.1, AC_000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6CGV | X-ray | 3.80 | W | 6-29 | [»] | |
SMRi | P24937 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24937 |
Family and domain databases
DisProti | DP00808 |
HAMAPi | MF_04048, ADV_CAP6, 1 hit |
InterProi | View protein in InterPro IPR004243, McpVI |
Pfami | View protein in Pfam PF02993, MCPVI, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAP6_ADE05 | |
Accessioni | P24937Primary (citable) accession number: P24937 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | March 1, 1992 | |
Last modified: | February 23, 2022 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families