UniProtKB - P24918 (NDUS1_NEUCR)
Protein
NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial
Gene
nuo78
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Functioni
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.
Catalytic activityi
- EC:7.1.1.2
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
- [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 68 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 79 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 82 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 96 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 128 | Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 132 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 135 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 141 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 182 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 185 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 188 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 232 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 4 iron, 4 sulfur cluster binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
GO - Biological processi
- ATP synthesis coupled electron transport Source: InterPro
Keywordsi
Molecular function | Oxidoreductase, Translocase |
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | 2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone |
Protein family/group databases
TCDBi | 3.D.1.6.2, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial (EC:7.1.1.2)Alternative name(s): Complex I-78kD Short name: CI-78kD |
Gene namesi | Name:nuo78 ORF Names:B17C10.90, NCU01765 |
Organismi | Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) |
Taxonomic identifieri | 367110 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:NCU01765 |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
Other locations
- respirasome Source: UniProtKB-KW
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 33 | Mitochondrion1 PublicationAdd BLAST | 33 | |
ChainiPRO_0000019974 | 34 – 744 | NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrialAdd BLAST | 711 |
Proteomic databases
PRIDEi | P24918 |
Interactioni
Subunit structurei
Complex I is composed of about 40 different subunits.
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 34 – 112 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 112 – 151 | 4Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 251 – 307 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 57 |
Sequence similaritiesi
Belongs to the complex I 75 kDa subunit family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
HOGENOMi | CLU_000422_11_6_1 |
InParanoidi | P24918 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd IPR015405, NuoG_C |
Pfami | View protein in Pfam PF00384, Molybdopterin, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit PF09326, NADH_dhqG_C, 1 hit |
SMARTi | View protein in SMART SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P24918-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRSTLSRSA WRTGRHQAAR NASRAFSATA QRPAEVELTI DGKKVSIEAG
60 70 80 90 100
SALIQACEKA GVTIPRYCYH EKLMIAGNCR MCLVEVEKVP KPVASCAWPV
110 120 130 140 150
QPGMVVKTNS PLTHKAREGV MEFLLANHPL DCPICDQGGE CDLQDQSMRY
160 170 180 190 200
GGDRGRFHEV GGKRAVEDKN MGPLIKTSMN RCIQCTRCVR FANDIAGAPE
210 220 230 240 250
LGSTGRGNDL QIGTYLEKNL DSELSGNVID LCPVGALTSK PYAFRARPWE
260 270 280 290 300
LKKTESIDVL DGLGSNIRVD TRGLEVMRIL PRLNDEVNEE WINDKTRFAC
310 320 330 340 350
DGLKTQRLTI PLVRREGKFE PASWDQALTE IAHAYQTLNP QGNEFKAIAG
360 370 380 390 400
QLTEVESLVA MKDLANRLGS ENLALDMPSG HKPLAHGVDV RSNYIFNSSI
410 420 430 440 450
VGIESADVIL LVGTNPRHEA AVLNARIRKQ WLRSDLEIGV VGQTWDSTFE
460 470 480 490 500
FEHLGTDHAA LQKALEGDFG KKLQSAKNPM IIVGSGVTDH GDANAFYETV
510 520 530 540 550
GKFVDSNASN FLTEEWNGYN VLQRAASRVG AFEVGFTVPS AEIAQTKPKF
560 570 580 590 600
VWLLGADEFN EADIPKDAFI VYQGHHGDRG AQIADIVLPG AAYTEKAGTY
610 620 630 640 650
VNTEGRVQMT RAATGLPGAA RTDWKILRAV SEYLGVRLPY DDVAQLRDRM
660 670 680 690 700
VEISPALSSY DIIEPPSLQQ LSKVQLVEQN QGATATNEPL KKVIENFYFT
710 720 730 740
DAISRSSPTM ARCSAAKKTG DPRTNFMAPG MEEDRPMGQI AYGA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 125 | L → P in CAA40828 (PubMed:1832016).Curated | 1 | |
Sequence conflicti | 152 | G → R in CAA40828 (PubMed:1832016).Curated | 1 | |
Sequence conflicti | 164 | R → Q in CAA40828 (PubMed:1832016).Curated | 1 | |
Sequence conflicti | 340 | P → A in CAA40828 (PubMed:1832016).Curated | 1 | |
Sequence conflicti | 379 – 388 | SGHKPLAHGV → FGPQTSCSWC in CAA40828 (PubMed:1832016).Curated | 10 | |
Sequence conflicti | 493 | A → R in CAA40828 (PubMed:1832016).Curated | 1 | |
Sequence conflicti | 527 – 534 | SRVGAFEV → PESAPSRL in CAA40828 (PubMed:1832016).Curated | 8 | |
Sequence conflicti | 666 – 667 | PS → SL in CAA40828 (PubMed:1832016).Curated | 2 | |
Sequence conflicti | 722 | P → S in CAA40828 (PubMed:1832016).Curated | 1 | |
Sequence conflicti | 727 – 729 | MAP → IGS in CAA40828 (PubMed:1832016).Curated | 3 | |
Sequence conflicti | 740 | I → Y in CAA40828 (PubMed:1832016).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57602 mRNA Translation: CAA40828.1 AL355926 Genomic DNA Translation: CAB91229.1 CM002237 Genomic DNA Translation: EAA27952.3 |
PIRi | S17664 T49428 |
RefSeqi | XP_957188.3, XM_952095.3 |
Genome annotation databases
EnsemblFungii | EAA27952; EAA27952; NCU01765 |
GeneIDi | 3873340 |
KEGGi | ncr:NCU01765 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57602 mRNA Translation: CAA40828.1 AL355926 Genomic DNA Translation: CAB91229.1 CM002237 Genomic DNA Translation: EAA27952.3 |
PIRi | S17664 T49428 |
RefSeqi | XP_957188.3, XM_952095.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein family/group databases
TCDBi | 3.D.1.6.2, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
Proteomic databases
PRIDEi | P24918 |
Genome annotation databases
EnsemblFungii | EAA27952; EAA27952; NCU01765 |
GeneIDi | 3873340 |
KEGGi | ncr:NCU01765 |
Organism-specific databases
VEuPathDBi | FungiDB:NCU01765 |
Phylogenomic databases
HOGENOMi | CLU_000422_11_6_1 |
InParanoidi | P24918 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd IPR015405, NuoG_C |
Pfami | View protein in Pfam PF00384, Molybdopterin, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit PF09326, NADH_dhqG_C, 1 hit |
SMARTi | View protein in SMART SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NDUS1_NEUCR | |
Accessioni | P24918Primary (citable) accession number: P24918 Secondary accession number(s): Q7RV66, Q9P6E0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families