UniProtKB - P24860 (CCNB1_MOUSE)
G2/mitotic-specific cyclin-B1
Ccnb1
Functioni
GO - Molecular functioni
- cyclin-dependent protein serine/threonine kinase activator activity Source: MGI
- cyclin-dependent protein serine/threonine kinase regulator activity Source: GO_Central
- patched binding Source: MGI
- protein-containing complex binding Source: MGI
- protein kinase binding Source: MGI
- ubiquitin-like protein ligase binding Source: MGI
GO - Biological processi
- cell division Source: UniProtKB-KW
- cellular response to fatty acid Source: Ensembl
- cellular response to hypoxia Source: Ensembl
- cellular response to iron(III) ion Source: Ensembl
- cellular response to organic cyclic compound Source: Ensembl
- digestive tract development Source: Ensembl
- histone H3-S10 phosphorylation involved in chromosome condensation Source: MGI
- in utero embryonic development Source: MGI
- mitotic cell cycle phase transition Source: GO_Central
- mitotic metaphase plate congression Source: MGI
- mitotic spindle organization Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of protein phosphorylation Source: MGI
- oocyte maturation Source: MGI
- positive regulation of attachment of spindle microtubules to kinetochore Source: MGI
- positive regulation of cardiac muscle cell proliferation Source: MGI
- positive regulation of cell cycle Source: MGI
- positive regulation of fibroblast proliferation Source: MGI
- positive regulation of G2/M transition of mitotic cell cycle Source: MGI
- positive regulation of histone phosphorylation Source: MGI
- positive regulation of mitochondrial ATP synthesis coupled electron transport Source: MGI
- positive regulation of mitotic cell cycle Source: MGI
- positive regulation of mRNA 3'-end processing Source: MGI
- protein-containing complex assembly Source: MGI
- protein phosphorylation Source: MGI
- regulation of chromosome condensation Source: MGI
- regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
- regulation of mitotic cell cycle spindle assembly checkpoint Source: MGI
- response to DDT Source: Ensembl
- response to drug Source: Ensembl
- response to mechanical stimulus Source: Ensembl
- spermatogenesis Source: Ensembl
- tissue regeneration Source: Ensembl
- ventricular cardiac muscle cell development Source: Ensembl
Keywordsi
Molecular function | Cyclin |
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
Reactomei | R-MMU-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-MMU-176408, Regulation of APC/C activators between G1/S and early anaphase R-MMU-176412, Phosphorylation of the APC/C R-MMU-176417, Phosphorylation of Emi1 R-MMU-2299718, Condensation of Prophase Chromosomes R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 R-MMU-2995383, Initiation of Nuclear Envelope (NE) Reformation R-MMU-3301854, Nuclear Pore Complex (NPC) Disassembly R-MMU-4419969, Depolymerisation of the Nuclear Lamina R-MMU-6804114, TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest R-MMU-68875, Mitotic Prophase R-MMU-69273, Cyclin A/B1/B2 associated events during G2/M transition R-MMU-69478, G2/M DNA replication checkpoint R-MMU-75035, Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex R-MMU-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-MMU-8878166, Transcriptional regulation by RUNX2 |
Names & Taxonomyi
Protein namesi | Recommended name: G2/mitotic-specific cyclin-B1 |
Gene namesi | Name:Ccnb1 Synonyms:Ccn-2, Ccnb1-rs13, Cycb, Cycb1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88302, Ccnb1 |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
Nucleus
Other locations
Cytoskeleton
- centrosome Source: UniProtKB
- spindle pole Source: MGI
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrial matrix Source: Ensembl
Nucleus
- condensed nuclear chromosome outer kinetochore Source: MGI
- nucleus Source: MGI
Other locations
- cyclin B1-CDK1 complex Source: MGI
- cyclin-dependent protein kinase holoenzyme complex Source: GO_Central
- cytoplasm Source: MGI
- membrane Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080352 | 1 – 430 | G2/mitotic-specific cyclin-B1Add BLAST | 430 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 73 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 123 | Phosphoserine; by CDK1By similarity | 1 | |
Modified residuei | 125 | PhosphoserineBy similarity | 1 | |
Modified residuei | 130 | Phosphoserine; by PLK1By similarity | 1 | |
Modified residuei | 144 | PhosphoserineBy similarity | 1 | |
Modified residuei | 318 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P24860 |
PaxDbi | P24860 |
PeptideAtlasi | P24860 |
PRIDEi | P24860 |
PTM databases
iPTMneti | P24860 |
PhosphoSitePlusi | P24860 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000041431, Expressed in embryo and 212 other tissues |
Genevisiblei | P24860, MM |
Interactioni
Subunit structurei
Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10.
Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.
Interacts with CCNF; interaction is required for nuclear localization.
Interacts with CDK5RAP3 (By similarity).
Interacts with RFPL4A and UBE2A (PubMed:12525704).
Interacts with INCA1 (By similarity).
By similarity1 PublicationGO - Molecular functioni
- patched binding Source: MGI
- protein kinase binding Source: MGI
- ubiquitin-like protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 234538, 11 interactors |
ComplexPortali | CPX-2069, Cyclin B1-CDK1 complex |
CORUMi | P24860 |
IntActi | P24860, 2 interactors |
MINTi | P24860 |
STRINGi | 10090.ENSMUSP00000071989 |
Miscellaneous databases
RNActi | P24860, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 166 – 174 | Interaction with CDK2By similarity | 9 | |
Regioni | 255 – 258 | Interaction with CDK2By similarity | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 51 – 85 | Lys-richAdd BLAST | 35 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0653, Eukaryota |
GeneTreei | ENSGT00940000154586 |
InParanoidi | P24860 |
OMAi | YLVGQEV |
OrthoDBi | 993640at2759 |
PhylomeDBi | P24860 |
TreeFami | TF101001 |
Family and domain databases
CDDi | cd00043, CYCLIN, 2 hits |
InterProi | View protein in InterPro IPR039361, Cyclin IPR013763, Cyclin-like IPR036915, Cyclin-like_sf IPR004367, Cyclin_C-dom IPR006671, Cyclin_N |
PANTHERi | PTHR10177, PTHR10177, 1 hit |
Pfami | View protein in Pfam PF02984, Cyclin_C, 1 hit PF00134, Cyclin_N, 1 hit |
PIRSFi | PIRSF001771, Cyclin_A_B_D_E, 1 hit |
SMARTi | View protein in SMART SM00385, CYCLIN, 2 hits SM01332, Cyclin_C, 1 hit |
SUPFAMi | SSF47954, SSF47954, 2 hits |
PROSITEi | View protein in PROSITE PS00292, CYCLINS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MALRVTRNTK INAENKAKVS MAGAKRVPVT VTAASKPGLR PRTALGDIGN
60 70 80 90 100
KVSEELQARV PLKREAKTLG TGKGTVKALP KPVEKVPVCE PEVELAEPEP
110 120 130 140 150
EPELEHVREE KLSPEPILVD NPSPSPMETS GCAPAEEYLC QAFSDVILAV
160 170 180 190 200
SDVDADDGAD PNLCSEYVKD IYAYLRQLEE EQSVRPKYLQ GREVTGNMRA
210 220 230 240 250
ILIDWLIQVQ MKFRLLQETM YMTVSIIDRF MQNSCVPKKM LQLVGVTAMF
260 270 280 290 300
IASKYEEMYP PEIGDFAFVT NNTYTKHQIR QMEMKILRVL NFSLGRPLPL
310 320 330 340 350
HFLRRASKVG EVDVEQHTLA KYLMELSMLD YDMVHFAPSQ IAAGAFCLAL
360 370 380 390 400
KILDNGEWTP TLQHYLSYSE DSLLPVMQHL AKNVVMVNCG LTKHMTVKNK
410 420 430
YAASKHAKIS TLAQLNCTLV QNLSKAVTKA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UY65 | G3UY65_MOUSE | G2/mitotic-specific cyclin-B1 | Ccnb1 | 393 | Annotation score: | ||
Q68EM3 | Q68EM3_MOUSE | Ccnb1 protein | Ccnb1 | 369 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 25 | K → M in CAA45968 (PubMed:1280449).Curated | 1 | |
Sequence conflicti | 59 | R → T in CAA41545 (PubMed:1836195).Curated | 1 | |
Sequence conflicti | 130 | S → C in CAA41545 (PubMed:1836195).Curated | 1 | |
Sequence conflicti | 139 | L → P in CAA41545 (PubMed:1836195).Curated | 1 | |
Sequence conflicti | 157 | D → S in CAA41545 (PubMed:1836195).Curated | 1 | |
Sequence conflicti | 241 | L → I in CAA41545 (PubMed:1836195).Curated | 1 | |
Sequence conflicti | 257 | E → D in CAA41545 (PubMed:1836195).Curated | 1 | |
Sequence conflicti | 315 | E → R in CAA41545 (PubMed:1836195).Curated | 1 | |
Sequence conflicti | 331 | Y → C in CAA45968 (PubMed:1280449).Curated | 1 | |
Sequence conflicti | 340 – 343 | QIAA → RAFS in CAA41545 (PubMed:1836195).Curated | 4 | |
Sequence conflicti | 351 | K → E in CAA41545 (PubMed:1836195).Curated | 1 | |
Sequence conflicti | 419 | L → H in CAA41545 (PubMed:1836195).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64713 mRNA Translation: CAA45968.1 X58708 mRNA Translation: CAA41545.1 S43105 mRNA Translation: AAB22970.1 BC011478 mRNA Translation: AAH11478.1 BC085238 mRNA Translation: AAH85238.1 |
CCDSi | CCDS36768.1 |
PIRi | A43285 I48316 JH0509 |
RefSeqi | NP_758505.2, NM_172301.3 |
Genome annotation databases
Ensembli | ENSMUST00000072119; ENSMUSP00000071989; ENSMUSG00000041431 |
GeneIDi | 268697 |
KEGGi | mmu:268697 |
UCSCi | uc007rro.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64713 mRNA Translation: CAA45968.1 X58708 mRNA Translation: CAA41545.1 S43105 mRNA Translation: AAB22970.1 BC011478 mRNA Translation: AAH11478.1 BC085238 mRNA Translation: AAH85238.1 |
CCDSi | CCDS36768.1 |
PIRi | A43285 I48316 JH0509 |
RefSeqi | NP_758505.2, NM_172301.3 |
3D structure databases
SMRi | P24860 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 234538, 11 interactors |
ComplexPortali | CPX-2069, Cyclin B1-CDK1 complex |
CORUMi | P24860 |
IntActi | P24860, 2 interactors |
MINTi | P24860 |
STRINGi | 10090.ENSMUSP00000071989 |
PTM databases
iPTMneti | P24860 |
PhosphoSitePlusi | P24860 |
Proteomic databases
EPDi | P24860 |
PaxDbi | P24860 |
PeptideAtlasi | P24860 |
PRIDEi | P24860 |
Protocols and materials databases
Antibodypediai | 3537, 1825 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000072119; ENSMUSP00000071989; ENSMUSG00000041431 |
GeneIDi | 268697 |
KEGGi | mmu:268697 |
UCSCi | uc007rro.1, mouse |
Organism-specific databases
CTDi | 891 |
MGIi | MGI:88302, Ccnb1 |
Phylogenomic databases
eggNOGi | KOG0653, Eukaryota |
GeneTreei | ENSGT00940000154586 |
InParanoidi | P24860 |
OMAi | YLVGQEV |
OrthoDBi | 993640at2759 |
PhylomeDBi | P24860 |
TreeFami | TF101001 |
Enzyme and pathway databases
Reactomei | R-MMU-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-MMU-176408, Regulation of APC/C activators between G1/S and early anaphase R-MMU-176412, Phosphorylation of the APC/C R-MMU-176417, Phosphorylation of Emi1 R-MMU-2299718, Condensation of Prophase Chromosomes R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 R-MMU-2995383, Initiation of Nuclear Envelope (NE) Reformation R-MMU-3301854, Nuclear Pore Complex (NPC) Disassembly R-MMU-4419969, Depolymerisation of the Nuclear Lamina R-MMU-6804114, TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest R-MMU-68875, Mitotic Prophase R-MMU-69273, Cyclin A/B1/B2 associated events during G2/M transition R-MMU-69478, G2/M DNA replication checkpoint R-MMU-75035, Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex R-MMU-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-MMU-8878166, Transcriptional regulation by RUNX2 |
Miscellaneous databases
BioGRID-ORCSi | 268697, 7 hits in 18 CRISPR screens |
ChiTaRSi | Ccnb1, mouse |
PROi | PR:P24860 |
RNActi | P24860, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000041431, Expressed in embryo and 212 other tissues |
Genevisiblei | P24860, MM |
Family and domain databases
CDDi | cd00043, CYCLIN, 2 hits |
InterProi | View protein in InterPro IPR039361, Cyclin IPR013763, Cyclin-like IPR036915, Cyclin-like_sf IPR004367, Cyclin_C-dom IPR006671, Cyclin_N |
PANTHERi | PTHR10177, PTHR10177, 1 hit |
Pfami | View protein in Pfam PF02984, Cyclin_C, 1 hit PF00134, Cyclin_N, 1 hit |
PIRSFi | PIRSF001771, Cyclin_A_B_D_E, 1 hit |
SMARTi | View protein in SMART SM00385, CYCLIN, 2 hits SM01332, Cyclin_C, 1 hit |
SUPFAMi | SSF47954, SSF47954, 2 hits |
PROSITEi | View protein in PROSITE PS00292, CYCLINS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CCNB1_MOUSE | |
Accessioni | P24860Primary (citable) accession number: P24860 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 191 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families