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Protein

Alkaline phosphatase, germ cell type

Gene

Alpg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Miscellaneous

In most mammals there are four different isozymes: placental, germ cell, intestinal and tissue non-specific (liver/bone/kidney).

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60MagnesiumBy similarity1
Metal bindingi60Zinc 1By similarity1
Active sitei110Phosphoserine intermediate1
Metal bindingi110Zinc 1By similarity1
Metal bindingi173MagnesiumBy similarity1
Metal bindingi329MagnesiumBy similarity1
Metal bindingi334Zinc 2By similarity1
Metal bindingi338Zinc 2; via tele nitrogenBy similarity1
Metal bindingi375Zinc 1By similarity1
Metal bindingi376Zinc 1; via tele nitrogenBy similarity1
Metal bindingi450Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1483166 Synthesis of PA
R-MMU-163125 Post-translational modification: synthesis of GPI-anchored proteins
R-MMU-8935690 Digestion

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, germ cell typeCurated (EC:3.1.3.1PROSITE-ProRule annotation)
Alternative name(s):
Alkaline phosphatase 5
Alkaline phosphatase, placental-like
Embryonic alkaline phosphatase
Short name:
EAP
Embryonic-type alkaline phosphatase
Gene namesi
Name:Alpg
Synonyms:Akp5, Alppl2Imported, Eap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:108009 Alppl2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3112374

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000002404519 – 502Alkaline phosphatase, germ cell typeAdd BLAST484
PropeptideiPRO_0000024046503 – 529Removed in mature formBy similarityAdd BLAST27

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi139 ↔ 201By similarity
Glycosylationi140N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi267N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi277N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi485 ↔ 492By similarity
Lipidationi502GPI-anchor amidated serineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP24823
PaxDbiP24823
PeptideAtlasiP24823
PRIDEiP24823

PTM databases

iPTMnetiP24823
PhosphoSitePlusiP24823

Expressioni

Tissue specificityi

Embryo and testis.

Gene expression databases

BgeeiENSMUSG00000026246 Expressed in 47 organ(s), highest expression level in embryo
CleanExiMM_AKP5
GenevisibleiP24823 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027455

Chemistry databases

BindingDBiP24823

Structurei

3D structure databases

ProteinModelPortaliP24823
SMRiP24823
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126 Eukaryota
COG1785 LUCA
GeneTreeiENSGT00390000008704
HOGENOMiHOG000099118
HOVERGENiHBG007345
InParanoidiP24823
KOiK01077
OMAiCKDISTQ
OrthoDBiEOG091G067H
TreeFamiTF323513

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 1 hit
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24823-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MWGACLLLLG LSLQVCPSVI PVEEENPAFW NRKAAEALDA AKKLKPIQTS
60 70 80 90 100
AKNLVILMGD GMGVSTVTAT RILKGQQQGH LGPETQLAMD RFPHMALSKT
110 120 130 140 150
YNTDKQIPDS AGTGTAFLCG VKTNMKVIGL SAAARFNQCN TTWGNEVVSV
160 170 180 190 200
MHRAKKAGKS VGVVTTTSVQ HASPAGTYAH TVNRGWYSDA QMPASALQDG
210 220 230 240 250
CKDISTQLIS NMDIDVILGG GRKFMFPKGT PDQEYPTDTK QAGTRLDGRN
260 270 280 290 300
LVQEWLAKHQ GARYVWNRSE LIQASLNRSV THLMGLFEPN DMKYEIHRDP
310 320 330 340 350
AQDPSLAEMT EVAVRMLSRN PKGFYLFVEG GRIDHGHHET VAYRALTEAV
360 370 380 390 400
MFDSAVDKAD KLTSEQDTMI LVTADHSHVF SFGGYTQRGA SIFGLAPFKA
410 420 430 440 450
EDGKSFTSIL YGNGPGYKLH NGARADVTEE ESSNPTYQQQ AAVPLSSETH
460 470 480 490 500
SGEDVAIFAR GPQAHLVHGV QEQNYIAHVM AFAACLEPYT DCGLASPAGQ
510 520
SSAVSPGYMS TLLCLLAGKM LMLMAAAEP
Length:529
Mass (Da):57,202
Last modified:July 27, 2011 - v2
Checksum:i540A95D21D90BDEC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80 – 81HL → LS in AAA37531 (PubMed:2286375).Curated2
Sequence conflicti361 – 362KL → IR in AAA37531 (PubMed:2286375).Curated2
Sequence conflicti442A → C in AAA37531 (PubMed:2286375).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61704 Genomic DNA Translation: AAA37531.1
AK145571 mRNA Translation: BAE26520.1
CH466520 Genomic DNA Translation: EDL40194.1
CCDSiCCDS15126.1
RefSeqiNP_031459.3, NM_007433.3
XP_006529145.1, XM_006529082.3
XP_011246206.1, XM_011247904.2
UniGeneiMm.195224

Genome annotation databases

EnsembliENSMUST00000027455; ENSMUSP00000027455; ENSMUSG00000026246
ENSMUST00000188310; ENSMUSP00000139887; ENSMUSG00000026246
GeneIDi11650
KEGGimmu:11650
UCSCiuc007bvy.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61704 Genomic DNA Translation: AAA37531.1
AK145571 mRNA Translation: BAE26520.1
CH466520 Genomic DNA Translation: EDL40194.1
CCDSiCCDS15126.1
RefSeqiNP_031459.3, NM_007433.3
XP_006529145.1, XM_006529082.3
XP_011246206.1, XM_011247904.2
UniGeneiMm.195224

3D structure databases

ProteinModelPortaliP24823
SMRiP24823
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027455

Chemistry databases

BindingDBiP24823
ChEMBLiCHEMBL3112374

PTM databases

iPTMnetiP24823
PhosphoSitePlusiP24823

Proteomic databases

MaxQBiP24823
PaxDbiP24823
PeptideAtlasiP24823
PRIDEiP24823

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027455; ENSMUSP00000027455; ENSMUSG00000026246
ENSMUST00000188310; ENSMUSP00000139887; ENSMUSG00000026246
GeneIDi11650
KEGGimmu:11650
UCSCiuc007bvy.1 mouse

Organism-specific databases

CTDi11650
MGIiMGI:108009 Alppl2

Phylogenomic databases

eggNOGiKOG4126 Eukaryota
COG1785 LUCA
GeneTreeiENSGT00390000008704
HOGENOMiHOG000099118
HOVERGENiHBG007345
InParanoidiP24823
KOiK01077
OMAiCKDISTQ
OrthoDBiEOG091G067H
TreeFamiTF323513

Enzyme and pathway databases

ReactomeiR-MMU-1483166 Synthesis of PA
R-MMU-163125 Post-translational modification: synthesis of GPI-anchored proteins
R-MMU-8935690 Digestion

Miscellaneous databases

PROiPR:P24823
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026246 Expressed in 47 organ(s), highest expression level in embryo
CleanExiMM_AKP5
GenevisibleiP24823 MM

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 1 hit
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPBN_MOUSE
AccessioniPrimary (citable) accession number: P24823
Secondary accession number(s): Q3ULC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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