UniProtKB - P24785 (MLE_DROME)
Protein
Dosage compensation regulator
Gene
mle
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Required in males for dosage compensation of X chromosome linked genes. Mle, msl-1 and msl-3 are colocalized on X chromosome. Each of the msl proteins requires all the other msls for wild-type X-chromosome binding. Probably unwinds double-stranded DNA and RNA in a 3' to 5' direction.1 Publication
Catalytic activityi
- EC:3.6.4.13
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 407 – 414 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- 3'-5' DNA helicase activity Source: GO_Central
- 3'-5' RNA helicase activity Source: CACAO
- ATP binding Source: UniProtKB-KW
- chromatin binding Source: FlyBase
- DNA helicase activity Source: UniProtKB
- double-stranded DNA binding Source: UniProtKB
- double-stranded RNA binding Source: UniProtKB
- helicase activity Source: FlyBase
- regulatory region RNA binding Source: UniProtKB
- RNA binding Source: FlyBase
- RNA helicase activity Source: FlyBase
GO - Biological processi
- axon extension Source: FlyBase
- determination of adult lifespan Source: FlyBase
- DNA duplex unwinding Source: UniProtKB
- dosage compensation Source: FlyBase
- dosage compensation by hyperactivation of X chromosome Source: FlyBase
- dosage compensation complex assembly involved in dosage compensation by hyperactivation of X chromosome Source: FlyBase
- male courtship behavior, veined wing generated song production Source: FlyBase
- positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity Source: UniProtKB
- positive regulation of heterochromatin assembly Source: FlyBase
- positive regulation of transcription by RNA polymerase II Source: FlyBase
- regulation of cytoplasmic translation Source: UniProtKB
- regulation of mRNA processing Source: UniProtKB
Keywordsi
Molecular function | Helicase, Hydrolase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-1810476, RIP-mediated NFkB activation via ZBP1 R-DME-3134963, DEx/H-box helicases activate type I IFN and inflammatory cytokines production R-DME-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Dosage compensation regulator (EC:3.6.4.13)Alternative name(s): ATP-dependent RNA helicase mle Protein male-less Protein maleless Protein no action potential |
Gene namesi | ORF Names:CG11680Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0002774, mle |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- Chromosome 1 Publication
Note: Mle is associated with hundreds of discrete sites along the length of the X chromosome in males and not in females, and is associated with 30-40 autosomal sites in both sexes.
Cytosol
- cytosol Source: FlyBase
Nucleus
- MSL complex Source: FlyBase
- nuclear chromosome Source: FlyBase
- nucleolus Source: GO_Central
- nucleus Source: FlyBase
- X chromosome located dosage compensation complex, transcription activating Source: FlyBase
Other locations
- chromatin Source: FlyBase
- chromosome Source: FlyBase
- cytoplasm Source: GO_Central
- polytene chromosome Source: FlyBase
- ribonucleoprotein complex Source: UniProtKB
- X chromosome Source: FlyBase
Keywords - Cellular componenti
Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055181 | 1 – 1293 | Dosage compensation regulatorAdd BLAST | 1293 |
Proteomic databases
PaxDbi | P24785 |
PRIDEi | P24785 |
Expressioni
Developmental stagei
Expressed throughout development; highest in embryos and at equal levels in males and females.1 Publication
Gene expression databases
Bgeei | FBgn0002774, Expressed in embryonic/larval hemocyte (Drosophila) and 36 other tissues |
ExpressionAtlasi | P24785, baseline and differential |
Genevisiblei | P24785, DM |
Interactioni
Subunit structurei
Interacts with Top2.
1 PublicationProtein-protein interaction databases
BioGRIDi | 61429, 39 interactors |
IntActi | P24785, 6 interactors |
STRINGi | 7227.FBpp0085367 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SASBDBi | P24785 |
SMRi | P24785 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 70 | DRBM 1PROSITE-ProRule annotationAdd BLAST | 69 | |
Domaini | 169 – 241 | DRBM 2PROSITE-ProRule annotationAdd BLAST | 73 | |
Domaini | 394 – 560 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 167 | |
Domaini | 639 – 813 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 175 | |
Repeati | 1202 – 1208 | 1 | 7 | |
Repeati | 1209 – 1215 | 2 | 7 | |
Repeati | 1216 – 1222 | 3 | 7 | |
Repeati | 1223 – 1229 | 4 | 7 | |
Repeati | 1230 – 1236 | 5 | 7 | |
Repeati | 1237 – 1243 | 6 | 7 | |
Repeati | 1244 – 1250 | 7 | 7 | |
Repeati | 1251 – 1257 | 8 | 7 | |
Repeati | 1258 – 1263 | 9 | 6 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1202 – 1263 | 9 X 7 AA tandem repeats of G-G-G-Y-G-N-NAdd BLAST | 62 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 507 – 510 | DEAH box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1173 – 1291 | Gly-richAdd BLAST | 119 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0921, Eukaryota |
GeneTreei | ENSGT00940000155924 |
InParanoidi | P24785 |
OMAi | HARSFMA |
PhylomeDBi | P24785 |
Family and domain databases
CDDi | cd00048, DSRM, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR002464, DNA/RNA_helicase_DEAH_CS IPR014720, dsRBD_dom IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00035, dsrm, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00358, DSRM, 2 hits SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS50137, DS_RBD, 2 hits PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
Isoform A (identifier: P24785-1) [UniParc]FASTAAdd to basket
Also known as: 25
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDIKSFLYQF CAKSQIEPKF DIRQTGPKNR QRFLCEVRVE PNTYIGVGNS
60 70 80 90 100
TNKKDAEKNA CRDFVNYLVR VGKLNTNDVP ADAGASGGGP RTGLEGAGMA
110 120 130 140 150
GGSGQQKRVF DGQSGPQDLG EAYRPLNHDG GDGGNRYSVI DRIQEQRDMN
160 170 180 190 200
EAEAFDVNAA IHGNWTIENA KERLNIYKQT NNIRDDYKYT PVGPEHARSF
210 220 230 240 250
LAELSIYVPA LNRTVTARES GSNKKSASKS CALSLVRQLF HLNVIEPFSG
260 270 280 290 300
TLKKKKDEQL KPYPVKLSPN LINKIDEVIK GLDLPVVNPR NIKIELDGPP
310 320 330 340 350
IPLIVNLSRI DSSQQDGEKR QESSVIPWAP PQANWNTWHA CNIDEGELAT
360 370 380 390 400
TSIDDLSMDY ERSLRDRRQN DNEYRQFLEF REKLPIAAMR SEILTAINDN
410 420 430 440 450
PVVIIRGNTG CGKTTQIAQY ILDDYICSGQ GGYANIYVTQ PRRISAISVA
460 470 480 490 500
ERVARERCEQ LGDTVGYSVR FESVFPRPYG AILFCTVGVL LRKLEAGLRG
510 520 530 540 550
VSHIIVDEIH ERDVNSDFLL VILRDMVDTY PDLHVILMSA TIDTTKFSKY
560 570 580 590 600
FGICPVLEVP GRAFPVQQFF LEDIIQMTDF VPSAESRRKR KEVEDEEQLL
610 620 630 640 650
SEDKDEAEIN YNKVCEDKYS QKTRNAMAML SESDVSFELL EALLMHIKSK
660 670 680 690 700
NIPGAILVFL PGWNLIFALM KFLQNTNIFG DTSQYQILPC HSQIPRDEQR
710 720 730 740 750
KVFEPVPEGV TKIILSTNIA ETSITIDDIV FVIDICKARM KLFTSHNNLT
760 770 780 790 800
SYATVWASKT NLEQRKGRAG RVRPGFCFTL CSRARFQALE DNLTPEMFRT
810 820 830 840 850
PLHEMALTIK LLRLGSIHHF LSKALEPPPV DAVIEAEVLL REMRCLDAND
860 870 880 890 900
ELTPLGRLLA RLPIEPRLGK MMVLGAVFGC ADLMAIMASY SSTFSEVFSL
910 920 930 940 950
DIGQRRLANH QKALSGTKCS DHVAMIVASQ MWRREKQRGE HMEARFCDWK
960 970 980 990 1000
GLQMSTMNVI WDAKQQLLDL LQQAGFPEEC MISHEVDERI DGDDPVLDVS
1010 1020 1030 1040 1050
LALLCLGLYP NICVHKEKRK VLTTESKAAL LHKTSVNCSN LAVTFPYPFF
1060 1070 1080 1090 1100
VFGEKIRTRA VSCKQLSMVS PLQVILFGSR KIDLAANNIV RVDNWLNFDI
1110 1120 1130 1140 1150
EPELAAKIGA LKPALEDLIT VACDNPSDIL RLEEPYAQLV KVVKDLCVKS
1160 1170 1180 1190 1200
AGDFGLQRES GILPHQSRQF SDGGGPPKRG RFETGRFTNS SFGRRGNGRT
1210 1220 1230 1240 1250
FGGGYGNNGG GYGNNGGGYG NIGGGYGNNA GGYGNNGGYG NNGGGYRNNG
1260 1270 1280 1290
GGYGNNGGGY GNKRGGFGDS FESNRGSGGG FRNGDQGGRW GNF
Isoform 12 (identifier: P24785-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
199-226: SFLAELSIYVPALNRTVTARESGSNKKS → YVLPFQLSSACISDLTRYGLRPNLKCSP
227-1293: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 590 | R → P in AAC41573 (PubMed:1653648).Curated | 1 | |
Sequence conflicti | 1263 | K → N in AAC41573 (PubMed:1653648).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 1276 | G → V. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015690 | 1 – 357 | Missing in isoform B. 1 PublicationAdd BLAST | 357 | |
Alternative sequenceiVSP_005775 | 199 – 226 | SFLAE…SNKKS → YVLPFQLSSACISDLTRYGL RPNLKCSP in isoform 12. 1 PublicationAdd BLAST | 28 | |
Alternative sequenceiVSP_005776 | 227 – 1293 | Missing in isoform 12. 1 PublicationAdd BLAST | 1067 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74121 mRNA Translation: AAC41573.1 AE013599 Genomic DNA Translation: AAF57297.1 AE013599 Genomic DNA Translation: AAM68335.1 BT003785 mRNA Translation: AAO41468.1 BT010267 mRNA Translation: AAQ23585.1 |
PIRi | B40025 |
RefSeqi | NP_476641.1, NM_057293.4 [P24785-1] NP_724440.1, NM_165451.3 [P24785-3] |
Genome annotation databases
EnsemblMetazoai | FBtr0086031; FBpp0085367; FBgn0002774 [P24785-1] FBtr0100576; FBpp0100031; FBgn0002774 [P24785-3] |
GeneIDi | 35523 |
KEGGi | dme:Dmel_CG11680 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74121 mRNA Translation: AAC41573.1 AE013599 Genomic DNA Translation: AAF57297.1 AE013599 Genomic DNA Translation: AAM68335.1 BT003785 mRNA Translation: AAO41468.1 BT010267 mRNA Translation: AAQ23585.1 |
PIRi | B40025 |
RefSeqi | NP_476641.1, NM_057293.4 [P24785-1] NP_724440.1, NM_165451.3 [P24785-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5AOR | X-ray | 2.08 | A/B | 1-1293 | [»] | |
5ZTM | X-ray | 2.90 | A/B | 1-264 | [»] | |
6I3R | NMR | - | A | 1-257 | [»] | |
SASBDBi | P24785 | |||||
SMRi | P24785 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 61429, 39 interactors |
IntActi | P24785, 6 interactors |
STRINGi | 7227.FBpp0085367 |
Proteomic databases
PaxDbi | P24785 |
PRIDEi | P24785 |
Genome annotation databases
EnsemblMetazoai | FBtr0086031; FBpp0085367; FBgn0002774 [P24785-1] FBtr0100576; FBpp0100031; FBgn0002774 [P24785-3] |
GeneIDi | 35523 |
KEGGi | dme:Dmel_CG11680 |
Organism-specific databases
CTDi | 35523 |
FlyBasei | FBgn0002774, mle |
Phylogenomic databases
eggNOGi | KOG0921, Eukaryota |
GeneTreei | ENSGT00940000155924 |
InParanoidi | P24785 |
OMAi | HARSFMA |
PhylomeDBi | P24785 |
Enzyme and pathway databases
Reactomei | R-DME-1810476, RIP-mediated NFkB activation via ZBP1 R-DME-3134963, DEx/H-box helicases activate type I IFN and inflammatory cytokines production R-DME-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
BioGRID-ORCSi | 35523, 0 hits in 1 CRISPR screen |
GenomeRNAii | 35523 |
PROi | PR:P24785 |
Gene expression databases
Bgeei | FBgn0002774, Expressed in embryonic/larval hemocyte (Drosophila) and 36 other tissues |
ExpressionAtlasi | P24785, baseline and differential |
Genevisiblei | P24785, DM |
Family and domain databases
CDDi | cd00048, DSRM, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR002464, DNA/RNA_helicase_DEAH_CS IPR014720, dsRBD_dom IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00035, dsrm, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00358, DSRM, 2 hits SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS50137, DS_RBD, 2 hits PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MLE_DROME | |
Accessioni | P24785Primary (citable) accession number: P24785 Secondary accession number(s): Q86NQ4, Q9V9J1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | September 27, 2005 | |
Last modified: | December 2, 2020 | |
This is version 194 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families