We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P24647 (IE2_NPVAC)
Protein
E3 ubiquitin-protein ligase IE2
Gene
IE2
Organism
Autographa californica nuclear polyhedrosis virus (AcMNPV)
Status
Functioni
RING-finger E3 ubiquitin ligase that plays an important regulatory role during the initial stages of infection. Migrates to specific nuclear foci early in infection supposely to prepare the sites for viral transcription and replication by targeting and ubiquitinating host proteins. Acts as a transcriptional activator and activates a number of viral promoters including itself, IE1 and the promoter of 39K gene.
By similarity2 PublicationsCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 207 – 255 | RING-typePROSITE-ProRule annotationAdd BLAST | 49 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- transferase activity Source: UniProtKB-KW
GO - Biological processi
- modulation by virus of host protein ubiquitination Source: UniProtKB-KW
- regulation of viral transcription Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding, Transferase |
Biological process | Host-virus interaction, Modulation of host ubiquitin pathway by viral E3 ligase, Modulation of host ubiquitin pathway by virus, Transcription, Transcription regulation, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase IE2 (EC:2.3.2.27)Alternative name(s): Immediate-early protein IE2 RING-type E3 ubiquitin transferase IE2Curated |
Gene namesi | Name:IE2 ORF Names:ORF151 |
Organismi | Autographa californica nuclear polyhedrosis virus (AcMNPV) |
Taxonomic identifieri | 46015 [NCBI] |
Taxonomic lineagei | Viruses › Naldaviricetes › Lefavirales › Baculoviridae › Alphabaculovirus › |
Virus hosti | Lepidoptera (butterflies and moths) [TaxID: 7088] |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056350 | 1 – 408 | E3 ubiquitin-protein ligase IE2Add BLAST | 408 |
Post-translational modificationi
Auto-ubiquitinated.By similarity
Keywords - PTMi
Ubl conjugationInteractioni
Subunit structurei
Homooligomer.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 34 – 41 | 1-1 | 8 | |
Repeati | 42 – 49 | 1-2 | 8 | |
Repeati | 51 – 54 | 2-1 | 4 | |
Repeati | 55 – 58 | 2-2 | 4 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 67 | DisorderedSequence analysisAdd BLAST | 67 | |
Regioni | 34 – 49 | 2 X 8 AA tandem repeats of Q-P-S-S-S-S-R-SAdd BLAST | 16 | |
Regioni | 51 – 58 | 2 X 4 AA tandem repeats of R-R-Q-E | 8 | |
Regioni | 176 – 199 | DisorderedSequence analysisAdd BLAST | 24 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 15 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 23 – 46 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 47 – 62 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Belongs to the alphabaculovirus IE2 protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 207 – 255 | RING-typePROSITE-ProRule annotationAdd BLAST | 49 |
Keywords - Domaini
Repeat, Zinc-fingerFamily and domain databases
InterProi | View protein in InterPro IPR018957, Znf_C3HC4_RING-type IPR001841, Znf_RING IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF00097, zf-C3HC4, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P24647-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRQINAATP SSSRRHRLSL SRRRINFTTS PEAQPSSSSR SQPSSSSRSH
60 70 80 90 100
RRQERRQEQR VSEENVQIIG NVNEPLTRTY HRQGVTYYVH GQVNISNDDP
110 120 130 140 150
LLSQEDDVIL INSENVDRER FPDITAQQYQ DNIASETAAQ RALQRGLDLE
160 170 180 190 200
AQLMNEIAPR SPTYSPSYSP NYVIPQSPDL FASPQSPQPQ QQQQQQSEPE
210 220 230 240 250
EEVEVSCNIC FTTFKDTKNV NSSFVTSIHC NHAVCFKCYV KIIMDNSVYK
260 270 280 290 300
CFCSATSSDC RVYNKHGYVE FMPINVTRNQ DSIKQHWREL LENNTVNNHT
310 320 330 340 350
TDLNYVEQLQ KELSELRAKT SQVEHKMTML NSDYIMLKHK HAVAELDLQK
360 370 380 390 400
ANYDLQESTK KSEELQSTVN NLQEQLRKQV AESQAKFSEF ERSNSDLVSK
LQTVMSRR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 138 | A → R in AAA46701 (PubMed:2024466).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59422 Genomic DNA Translation: AAA46701.1 L22858 Genomic DNA Translation: AAA66781.1 |
PIRi | A39150, WMNVIA A72869 |
RefSeqi | NP_054182.1, NC_001623.1 |
Genome annotation databases
GeneIDi | 1403984 |
KEGGi | vg:1403984 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59422 Genomic DNA Translation: AAA46701.1 L22858 Genomic DNA Translation: AAA66781.1 |
PIRi | A39150, WMNVIA A72869 |
RefSeqi | NP_054182.1, NC_001623.1 |
3D structure databases
SMRi | P24647 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 1403984 |
KEGGi | vg:1403984 |
Family and domain databases
InterProi | View protein in InterPro IPR018957, Znf_C3HC4_RING-type IPR001841, Znf_RING IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF00097, zf-C3HC4, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IE2_NPVAC | |
Accessioni | P24647Primary (citable) accession number: P24647 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | November 1, 1995 | |
Last modified: | September 29, 2021 | |
This is version 96 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families