UniProtKB - P24605 (PA2H2_BOTAS)
Protein
Basic phospholipase A2 homolog 2
Gene
N/A
Organism
Bothrops asper (Terciopelo)
Status
Functioni
Snake venom phospholipase A2 homolog that lacks enzymatic activity. Is myotoxic, induces a dose-dependent edema in the mouse foot pad, and exhibits strong anticoagulant effects by binding to factor Xa (F10) and inhibiting the prothrombinase activity (IC50 is 3 nM).4 Publications
Caution
Does not bind calcium as one of the calcium-binding sites is lost (Asp->Lys in position 64, which corresponds to 'Lys-49' in the current nomenclature).Curated
Activity regulationi
Suramin inhibits the myotoxic activity.1 Publication
GO - Molecular functioni
- calcium ion binding Source: InterPro
- phospholipase A2 activity Source: InterPro
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- arachidonic acid secretion Source: InterPro
- defense response to bacterium Source: UniProtKB-KW
- lipid catabolic process Source: InterPro
- phospholipid metabolic process Source: InterPro
Keywordsi
Molecular function | Antibiotic, Antimicrobial, Blood coagulation cascade inhibiting toxin, Hemostasis impairing toxin, Myotoxin, Toxin |
Names & Taxonomyi
Protein namesi | Recommended name: Basic phospholipase A2 homolog 2Short name: svPLA2 homolog Alternative name(s): Myotoxin II Short name: MtxII |
Organismi | Bothrops asper (Terciopelo) |
Taxonomic identifieri | 8722 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Viperidae › Crotalinae › Bothrops |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | 1 PublicationAdd BLAST | 16 | |
ChainiPRO_0000161618 | 17 – 137 | Basic phospholipase A2 homolog 2Add BLAST | 121 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 42 ↔ 131 | 1 Publication | ||
Disulfide bondi | 44 ↔ 60 | 1 Publication | ||
Disulfide bondi | 59 ↔ 111 | 1 Publication | ||
Disulfide bondi | 65 ↔ 137 | 1 Publication | ||
Disulfide bondi | 66 ↔ 104 | 1 Publication | ||
Disulfide bondi | 73 ↔ 97 | 1 Publication | ||
Disulfide bondi | 91 ↔ 102 | 1 Publication |
Post-translational modificationi
It binds long-chain fatty acids covalently by a rapid, spontaneous, and autocatalytic process. When acylated, it binds to the surface of liposomes and isolated muscle membranes, with the fatty acid moiety inserted into the lipid bilayer and possibly acting as an anchor (PubMed:7718570).1 Publication
Keywords - PTMi
Disulfide bond, LipoproteinExpressioni
Tissue specificityi
Expressed by the venom gland.
Interactioni
Subunit structurei
Dimer.4 Publications
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CLP | X-ray | 2.80 | A/B | 17-137 | [»] | |
1Y4L | X-ray | 1.70 | A/B | 17-137 | [»] | |
ProteinModelPortali | P24605 | |||||
SMRi | P24605 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24605 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 121 – 133 | Important for membrane-damaging activities in eukaryotes and bacteriaAdd BLAST | 13 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
HOVERGENi | HBG008137 |
Family and domain databases
CDDi | cd00125 PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211 PLipase_A2 IPR033112 PLipase_A2_Asp_AS IPR016090 PLipase_A2_dom IPR036444 PLipase_A2_dom_sf IPR033113 PLipase_A2_His_AS |
PANTHERi | PTHR11716 PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068 Phospholip_A2_1, 1 hit |
PRINTSi | PR00389 PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085 PA2c, 1 hit |
SUPFAMi | SSF48619 SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119 PA2_ASP, 1 hit PS00118 PA2_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P24605-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRTLWIMAVL LVGVEGSLFE LGKMILQETG KNPAKSYGAY GCNCGVLGRG
60 70 80 90 100
KPKDATDRCC YVHKCCYKKL TGCNPKKDRY SYSWKDKTIV CGENNSCLKE
110 120 130
LCECDKAVAI CLRENLNTYN KKYRYYLKPL CKKADAC
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 130 | L → F. | 1 |
Sequence databases
PIRi | S13900 |
Similar proteinsi
Cross-referencesi
Sequence databases
PIRi | S13900 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CLP | X-ray | 2.80 | A/B | 17-137 | [»] | |
1Y4L | X-ray | 1.70 | A/B | 17-137 | [»] | |
ProteinModelPortali | P24605 | |||||
SMRi | P24605 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protein family/group databases
Allergomei | 6319 Bot as 1 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Phylogenomic databases
HOVERGENi | HBG008137 |
Miscellaneous databases
EvolutionaryTracei | P24605 |
Family and domain databases
CDDi | cd00125 PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211 PLipase_A2 IPR033112 PLipase_A2_Asp_AS IPR016090 PLipase_A2_dom IPR036444 PLipase_A2_dom_sf IPR033113 PLipase_A2_His_AS |
PANTHERi | PTHR11716 PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068 Phospholip_A2_1, 1 hit |
PRINTSi | PR00389 PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085 PA2c, 1 hit |
SUPFAMi | SSF48619 SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119 PA2_ASP, 1 hit PS00118 PA2_HIS, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | PA2H2_BOTAS | |
Accessioni | P24605Primary (citable) accession number: P24605 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | January 15, 2008 | |
Last modified: | September 12, 2018 | |
This is version 104 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references