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Protein

DNA polymerase epsilon subunit B

Gene

DPB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.1 Publication

Miscellaneous

Present with 3110 +/- 251 molecules/cell in log phase SD medium.1 Publication
In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA-dependent DNA replication Source: SGD
  • DNA-dependent DNA replication maintenance of fidelity Source: SGD
  • error-prone translesion synthesis Source: SGD
  • heterochromatin organization involved in chromatin silencing Source: SGD

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processCell cycle, DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-34302-MONOMER
ReactomeiR-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-174430 Telomere C-strand synthesis initiation
R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SCE-5656169 Termination of translesion DNA synthesis
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER
R-SCE-68952 DNA replication initiation
R-SCE-68962 Activation of the pre-replicative complex

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit B (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit 2
Gene namesi
Name:DPB2
Ordered Locus Names:YPR175W
ORF Names:P9705.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR175W
SGDiS000006379 DPB2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000715731 – 689DNA polymerase epsilon subunit BAdd BLAST689

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei122PhosphoserineCombined sources1
Modified residuei141Phosphoserine; by CDC281 Publication1
Modified residuei613Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated in a cell cycle dependent manner during late G1 phase. Phosphorylation may facilitate the interaction with POL2 or the activity of DNA polymerase II. Phosphorylation is independent of DNA replication but dependent upon CDC28 in vivo. Both Ser-141 and Ser-613 are phosphorylated in vivo, but in vitro only Ser-141 is phosphorylated by CDC28.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP24482
PaxDbiP24482
PRIDEiP24482

PTM databases

iPTMnetiP24482

Interactioni

Subunit structurei

DNA polymerase epsilon is a heterotetramer consisting of POL2, DPB2, DPB3 and DPB4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
POL2P219517EBI-6071,EBI-6140

Protein-protein interaction databases

BioGridi36348, 68 interactors
ComplexPortaliCPX-2110 DNA polymerase epsilon complex
DIPiDIP-2533N
IntActiP24482, 25 interactors
MINTiP24482
STRINGi4932.YPR175W

Structurei

3D structure databases

ProteinModelPortaliP24482
SMRiP24482
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000012435
HOGENOMiHOG000246706
InParanoidiP24482
KOiK02325
OMAiFVPGDND
OrthoDBiEOG092C2QT9

Family and domain databases

InterProiView protein in InterPro
IPR007185 DNA_pol_alpha/epsilon_bsu
PfamiView protein in Pfam
PF04042 DNA_pol_E_B, 1 hit

Sequencei

Sequence statusi: Complete.

P24482-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFGSGNVLPV KIQPPLLRPL AYRVLSRKYG LSIKSDGLSA LAEFVGTNIG
60 70 80 90 100
ANWRQGPATI KFLEQFAAVW KQQERGLFID QSGVKEVIQE MKEREKVEWS
110 120 130 140 150
HEHPIQHEEN ILGRTDDDEN NSDDEMPIAA DSSLQNVSLS SPMRQPTERD
160 170 180 190 200
EYKQPFKPES SKALDWRDYF KVINASQQQR FSYNPHKMQF IFVPNKKQNG
210 220 230 240 250
LGGIAGFLPD IEDKVQMFLT RYYLTNDRVM RNENFQNSDM FNPLSSMVSL
260 270 280 290 300
QNELSNTNRQ QQSSSMSITP IKNLLGRDAQ NFLLLGLLNK NFKGNWSLED
310 320 330 340 350
PSGSVEIDIS QTIPTQGHYY VPGCMVLVEG IYYSVGNKFH VTSMTLPPGE
360 370 380 390 400
RREITLETIG NLDLLGIHGI SNNNFIARLD KDLKIRLHLL EKELTDHKFV
410 420 430 440 450
ILGANLFLDD LKIMTALSKI LQKLNDDPPT LLIWQGSFTS VPVFASMSSR
460 470 480 490 500
NISSSTQFKN NFDALATLLS RFDNLTENTT MIFIPGPNDL WGSMVSLGAS
510 520 530 540 550
GTLPQDPIPS AFTKKINKVC KNVVWSSNPT RIAYLSQEIV IFRDDLSGRF
560 570 580 590 600
KRHRLEFPFN ESEDVYTEND NMMSKDTDIV PIDELVKEPD QLPQKVQETR
610 620 630 640 650
KLVKTILDQG HLSPFLDSLR PISWDLDHTL TLCPIPSTMV LCDTTSAQFD
660 670 680
LTYNGCKVIN PGSFIHNRRA RYMEYVPSSK KTIQEEIYI
Length:689
Mass (Da):78,340
Last modified:January 10, 2006 - v3
Checksum:i0936E3FC90552788
GO

Sequence cautioni

The sequence AAA34576 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAB68109 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti458F → Y in AAA34576 (PubMed:2052544).Curated1
Sequence conflicti521K → R in AAA34576 (PubMed:2052544).Curated1
Sequence conflicti565V → F in AAA34576 (PubMed:2052544).Curated1
Sequence conflicti584E → Q in AAA34576 (PubMed:2052544).Curated1
Sequence conflicti644T → I in AAA34576 (PubMed:2052544).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61710 Genomic DNA Translation: AAA34576.1 Different initiation.
U25842 Genomic DNA Translation: AAB68109.1 Different initiation.
BK006949 Genomic DNA Translation: DAA11592.1
PIRiS59833
RefSeqiNP_015501.2, NM_001184272.1

Genome annotation databases

EnsemblFungiiYPR175W; YPR175W; YPR175W
GeneIDi856305
KEGGisce:YPR175W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61710 Genomic DNA Translation: AAA34576.1 Different initiation.
U25842 Genomic DNA Translation: AAB68109.1 Different initiation.
BK006949 Genomic DNA Translation: DAA11592.1
PIRiS59833
RefSeqiNP_015501.2, NM_001184272.1

3D structure databases

ProteinModelPortaliP24482
SMRiP24482
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36348, 68 interactors
ComplexPortaliCPX-2110 DNA polymerase epsilon complex
DIPiDIP-2533N
IntActiP24482, 25 interactors
MINTiP24482
STRINGi4932.YPR175W

PTM databases

iPTMnetiP24482

Proteomic databases

MaxQBiP24482
PaxDbiP24482
PRIDEiP24482

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR175W; YPR175W; YPR175W
GeneIDi856305
KEGGisce:YPR175W

Organism-specific databases

EuPathDBiFungiDB:YPR175W
SGDiS000006379 DPB2

Phylogenomic databases

GeneTreeiENSGT00390000012435
HOGENOMiHOG000246706
InParanoidiP24482
KOiK02325
OMAiFVPGDND
OrthoDBiEOG092C2QT9

Enzyme and pathway databases

BioCyciYEAST:G3O-34302-MONOMER
ReactomeiR-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-174430 Telomere C-strand synthesis initiation
R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SCE-5656169 Termination of translesion DNA synthesis
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER
R-SCE-68952 DNA replication initiation
R-SCE-68962 Activation of the pre-replicative complex

Miscellaneous databases

PROiPR:P24482

Family and domain databases

InterProiView protein in InterPro
IPR007185 DNA_pol_alpha/epsilon_bsu
PfamiView protein in Pfam
PF04042 DNA_pol_E_B, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPB2_YEAST
AccessioniPrimary (citable) accession number: P24482
Secondary accession number(s): D6W4H6, Q06622
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 10, 2006
Last modified: October 10, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
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Main funding by: National Institutes of Health

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