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Protein

Protein Wnt-7a

Gene

Wnt7a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:18230341, PubMed:20530549, PubMed:23629626). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed:7885472, PubMed:9769174, PubMed:9790192). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:28803732). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (PubMed:9790192). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (PubMed:23629626). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (PubMed:23629626). Promotes formation of synapses via its interaction with FZD5 (PubMed:20530549).7 Publications

Miscellaneous

Male mice lacking Wnt7a fail to undergo regression of the Mullerian duct as a result of the absence of the receptor for Mullerian-inhibiting substance. In males, mature sperm fills the vas deferens, but sperm exit is blocked due to the persistence of the Mullerian duct, causing male sterility. Wnt7a deficient females are infertile because of abnormal development of the oviduct and uterus, both of which are Mullerian duct derivatives.1 Publication

GO - Molecular functioni

  • cytokine activity Source: MGI
  • frizzled binding Source: MGI
  • receptor ligand activity Source: MGI
  • signaling receptor binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-3238698 WNT ligand biogenesis and trafficking

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-7a
Gene namesi
Name:Wnt7a
Synonyms:Wnt-7a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:98961 Wnt7a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Involvement in diseasei

Defects in Wnt7a cause the postaxial hemimelia (px) phenotype that is characterized by limb patterning defects accompanied by Mullerian duct-associated sterility in both sexes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000004144332 – 349Protein Wnt-7aAdd BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi73 ↔ 84By similarity
Glycosylationi83N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi123 ↔ 131By similarity
Glycosylationi127N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi133 ↔ 152By similarity
Disulfide bondi200 ↔ 214By similarity
Disulfide bondi202 ↔ 209By similarity
Lipidationi206O-palmitoleoyl serine; by PORCNBy similarity1
Disulfide bondi294 ↔ 309By similarity
Glycosylationi295N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi324 ↔ 339By similarity
Disulfide bondi326 ↔ 336By similarity
Disulfide bondi331 ↔ 332By similarity

Post-translational modificationi

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiP24383
PRIDEiP24383

PTM databases

PhosphoSitePlusiP24383

Expressioni

Developmental stagei

Detected in the flanking ectoderm of the trunk prior to limb outgrowth. First detected in the presumptive forelimb region at 8.75 dpc, and in the presumptive hindlimb region at 9.25 dpc. Uniformly distributed throughout the dorsal limb ectoderm during the initial stages of limb-bud outgrowth (9.25 dpc for the forelimbs, 9.75 dpc for the hindlimbs).1 Publication

Gene expression databases

BgeeiENSMUSG00000030093 Expressed in 185 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_WNT7A
GenevisibleiP24383 MM

Interactioni

Subunit structurei

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (By similarity). The complex with AFM may represent the physiological form in body fluids (By similarity). Interacts with FZD5 (PubMed:18230341, PubMed:20530549). Interacts with PORCN (PubMed:10866835). Interacts (via intrinsically disordered linker region) with RECK; interaction with RECK confers ligand selectivity for Wnt7 in brain endothelial cells and allows these cells to selectively respond to Wnt7 (By similarity).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204580, 4 interactors
STRINGi10090.ENSMUSP00000032180

Structurei

3D structure databases

ProteinModelPortaliP24383
SMRiP24383
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni238 – 266Intrinsically disordered linkerBy similarityAdd BLAST29

Domaini

The intrinsically disordered linker region is required for recognition by RECK in brain endothelial cells.By similarity

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913 Eukaryota
ENOG410XQZ1 LUCA
GeneTreeiENSGT00760000118943
HOGENOMiHOG000039528
HOVERGENiHBG001595
InParanoidiP24383
KOiK00572
OMAiKNMRLEC
OrthoDBiEOG091G0OFF
TreeFamiTF105310

Family and domain databases

InterProiView protein in InterPro
IPR005817 Wnt
IPR013300 Wnt7
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 1 hit
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01891 WNT7PROTEIN
PR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24383-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTRKARRCLG HLFLSLGIVY LRIGGFSSVV ALGASIICNK IPGLAPRQRA
60 70 80 90 100
ICQSRPDAII VIGEGSQMGL DECQFQFRNG RWNCSALGER TVFGKELKVG
110 120 130 140 150
SREAAFTYAI IAAGVAHAIT AACTQGNLSD CGCDKEKQGQ YHRDEGWKWG
160 170 180 190 200
GCSADIRYGI GFAKVFVDAR EIKQNARTLM NLHNNEAGRK ILEENMKLEC
210 220 230 240 250
KCHGVSGSCT TKTCWTTLPQ FRELGYVLKD KYNEAVHVEP VRASRNKRPT
260 270 280 290 300
FLKIKKPLSY RKPMDTDLVY IEKSPNYCEE DPVTGSVGTQ GRACNKTAPQ
310 320 330 340
ASGCDLMCCG RGYNTHQYAR VWQCNCKFHW CCYVKCNTCS ERTEMYTCK
Length:349
Mass (Da):38,974
Last modified:June 28, 2011 - v2
Checksum:iC36ACDF7930790A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143R → W in AAA40570 (PubMed:2279700).Curated1
Sequence conflicti273K → L in AAA40570 (PubMed:2279700).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89801 mRNA Translation: AAA40570.1
AK004683 mRNA Translation: BAB23470.1
CH466523 Genomic DNA Translation: EDK99298.1
BC049093 mRNA Translation: AAH49093.2
BC057586 mRNA Translation: AAH57586.1
CCDSiCCDS39568.1
PIRiG36470
RefSeqiNP_033553.2, NM_009527.3
UniGeneiMm.56964

Genome annotation databases

EnsembliENSMUST00000032180; ENSMUSP00000032180; ENSMUSG00000030093
GeneIDi22421
KEGGimmu:22421
UCSCiuc009cxz.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89801 mRNA Translation: AAA40570.1
AK004683 mRNA Translation: BAB23470.1
CH466523 Genomic DNA Translation: EDK99298.1
BC049093 mRNA Translation: AAH49093.2
BC057586 mRNA Translation: AAH57586.1
CCDSiCCDS39568.1
PIRiG36470
RefSeqiNP_033553.2, NM_009527.3
UniGeneiMm.56964

3D structure databases

ProteinModelPortaliP24383
SMRiP24383
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204580, 4 interactors
STRINGi10090.ENSMUSP00000032180

PTM databases

PhosphoSitePlusiP24383

Proteomic databases

PaxDbiP24383
PRIDEiP24383

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032180; ENSMUSP00000032180; ENSMUSG00000030093
GeneIDi22421
KEGGimmu:22421
UCSCiuc009cxz.1 mouse

Organism-specific databases

CTDi7476
MGIiMGI:98961 Wnt7a

Phylogenomic databases

eggNOGiKOG3913 Eukaryota
ENOG410XQZ1 LUCA
GeneTreeiENSGT00760000118943
HOGENOMiHOG000039528
HOVERGENiHBG001595
InParanoidiP24383
KOiK00572
OMAiKNMRLEC
OrthoDBiEOG091G0OFF
TreeFamiTF105310

Enzyme and pathway databases

ReactomeiR-MMU-3238698 WNT ligand biogenesis and trafficking

Miscellaneous databases

PROiPR:P24383
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030093 Expressed in 185 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_WNT7A
GenevisibleiP24383 MM

Family and domain databases

InterProiView protein in InterPro
IPR005817 Wnt
IPR013300 Wnt7
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 1 hit
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01891 WNT7PROTEIN
PR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWNT7A_MOUSE
AccessioniPrimary (citable) accession number: P24383
Secondary accession number(s): Q80VH3, Q9DBY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: June 28, 2011
Last modified: November 7, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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