UniProtKB - P24327 (PRSA_BACSU)
Protein
Foldase protein PrsA
Gene
prsA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Essential protein that plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins. Has PPIase activity but it is not essential for its function in vivo.1 Publication
Catalytic activityi
- EC:5.2.1.8
GO - Molecular functioni
- peptidyl-prolyl cis-trans isomerase activity Source: UniProtKB-UniRule
GO - Biological processi
- protein folding Source: UniProtKB-UniRule
Keywordsi
Molecular function | Isomerase, Rotamase |
Enzyme and pathway databases
BioCyci | BSUB:BSU09950-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:prsA Ordered Locus Names:BSU09950 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
- Cell membrane Curated; Lipid-anchor Curated
GO - Cellular componenti
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 20 | C → Y: Abolishes lipid modification of the protein. 1 Publication | 1 | |
Mutagenesisi | 141 | H → A: Loss of PPIase function. No effect on secretion of proteins and on growth. 1 Publication | 1 | |
Mutagenesisi | 163 | F → Y: No effect on secretion of proteins and on growth. 1 Publication | 1 | |
Mutagenesisi | 171 | S → A: No effect on secretion of proteins and on growth. 1 Publication | 1 | |
Mutagenesisi | 173 | D → A: Decrease in PPIase activity. No effect on secretion of proteins and on growth. 1 Publication | 1 | |
Mutagenesisi | 175 | S → P: No effect on secretion of proteins and on growth. | 1 | |
Mutagenesisi | 180 | G → A: No effect on secretion of proteins and on growth. 1 Publication | 1 | |
Mutagenesisi | 191 | M → A: No effect on secretion of proteins and on growth. 1 Publication | 1 | |
Mutagenesisi | 195 | F → A or Y: No effect on secretion of proteins and on growth. 1 Publication | 1 | |
Mutagenesisi | 215 | Y → A: No effect on secretion of proteins and on growth. 1 Publication | 1 | |
Mutagenesisi | 216 | G → A: Decreased secretion of proteins; no effect on growth. 1 Publication | 1 | |
Mutagenesisi | 218 | H → A: Decreased secretion of proteins; no effect on growth. 1 Publication | 1 | |
Mutagenesisi | 219 | I → A: No effect on secretion of proteins and on growth. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000029299 | 20 – 292 | Foldase protein PrsAAdd BLAST | 273 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 20 | N-palmitoyl cysteineSequence analysis | 1 | |
Lipidationi | 20 | S-diacylglycerol cysteineSequence analysis | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
jPOSTi | P24327 |
PaxDbi | P24327 |
PRIDEi | P24327 |
Interactioni
Protein-protein interaction databases
IntActi | P24327, 3 interactors |
MINTi | P24327 |
STRINGi | 224308.BSU09950 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P24327 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24327 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 134 – 224 | PpiCAdd BLAST | 91 |
Domaini
All three domains (PPIase domain and the flanking N- and C-terminal domains) are essential for activity.
Sequence similaritiesi
Belongs to the PrsA family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0760, Bacteria |
InParanoidi | P24327 |
OMAi | PGKMVKE |
PhylomeDBi | P24327 |
Family and domain databases
HAMAPi | MF_01145, Foldase_PrsA, 1 hit |
InterProi | View protein in InterPro IPR023059, Foldase_PrsA IPR000297, PPIase_PpiC IPR023058, PPIase_PpiC_CS IPR027304, Trigger_fact/SurA_dom_sf |
SUPFAMi | SSF109998, SSF109998, 1 hit |
PROSITEi | View protein in PROSITE PS01096, PPIC_PPIASE_1, 1 hit PS50198, PPIC_PPIASE_2, 1 hit PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P24327-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKIAIAAIT ATSILALSAC SSGDKEVIAK TDAGDVTKGE LYTNMKKTAG
60 70 80 90 100
ASVLTQLVQE KVLDKKYKVS DKEIDNKLKE YKTQLGDQYT ALEKQYGKDY
110 120 130 140 150
LKEQVKYELL TQKAAKDNIK VTDADIKEYW EGLKGKIRAS HILVADKKTA
160 170 180 190 200
EEVEKKLKKG EKFEDLAKEY STDSSASKGG DLGWFAKEGQ MDETFSKAAF
210 220 230 240 250
KLKTGEVSDP VKTQYGYHII KKTEERGKYD DMKKELKSEV LEQKLNDNAA
260 270 280 290
VQEAVQKVMK KADIEVKDKD LKDTFNTSST SNSTSSSSSN SK
Sequence cautioni
The sequence AAA22825 differs from that shown. Sequencing errors. The N-terminus of this sequence is probably the N-terminus of phrI.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57271 Genomic DNA Translation: CAA40543.1 Y14077 Genomic DNA Translation: CAA74418.1 AL009126 Genomic DNA Translation: CAB12835.1 M22909 Genomic DNA Translation: AAA22825.1 Sequence problems. |
PIRi | S15269 |
RefSeqi | NP_388876.1, NC_000964.3 WP_003245079.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB12835; CAB12835; BSU09950 |
GeneIDi | 939294 |
KEGGi | bsu:BSU09950 |
PATRICi | fig|224308.179.peg.1068 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57271 Genomic DNA Translation: CAA40543.1 Y14077 Genomic DNA Translation: CAA74418.1 AL009126 Genomic DNA Translation: CAB12835.1 M22909 Genomic DNA Translation: AAA22825.1 Sequence problems. |
PIRi | S15269 |
RefSeqi | NP_388876.1, NC_000964.3 WP_003245079.1, NZ_JNCM01000035.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZK6 | NMR | - | A | 135-225 | [»] | |
4WO7 | X-ray | 2.63 | A/B | 21-280 | [»] | |
SMRi | P24327 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P24327, 3 interactors |
MINTi | P24327 |
STRINGi | 224308.BSU09950 |
Proteomic databases
jPOSTi | P24327 |
PaxDbi | P24327 |
PRIDEi | P24327 |
Genome annotation databases
EnsemblBacteriai | CAB12835; CAB12835; BSU09950 |
GeneIDi | 939294 |
KEGGi | bsu:BSU09950 |
PATRICi | fig|224308.179.peg.1068 |
Phylogenomic databases
eggNOGi | COG0760, Bacteria |
InParanoidi | P24327 |
OMAi | PGKMVKE |
PhylomeDBi | P24327 |
Enzyme and pathway databases
BioCyci | BSUB:BSU09950-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P24327 |
Family and domain databases
HAMAPi | MF_01145, Foldase_PrsA, 1 hit |
InterProi | View protein in InterPro IPR023059, Foldase_PrsA IPR000297, PPIase_PpiC IPR023058, PPIase_PpiC_CS IPR027304, Trigger_fact/SurA_dom_sf |
SUPFAMi | SSF109998, SSF109998, 1 hit |
PROSITEi | View protein in PROSITE PS01096, PPIC_PPIASE_1, 1 hit PS50198, PPIC_PPIASE_2, 1 hit PS51257, PROKAR_LIPOPROTEIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRSA_BACSU | |
Accessioni | P24327Primary (citable) accession number: P24327 Secondary accession number(s): Q45680 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | March 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families