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Protein

Alanine aminotransferase 1

Gene

GPT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).By similarity

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pathwayi: L-alanine degradation via transaminase pathway

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine aminotransferase 2 (GPT2), Alanine aminotransferase 1 (GPT)
This subpathway is part of the pathway L-alanine degradation via transaminase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from L-alanine, the pathway L-alanine degradation via transaminase pathway and in Amino-acid degradation.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:HS09610-MONOMER
ReactomeiR-HSA-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayi
UPA00528;UER00586

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine aminotransferase 1 (EC:2.6.1.2)
Short name:
ALT1
Alternative name(s):
Glutamate pyruvate transaminase 1
Short name:
GPT 1
Glutamic--alanine transaminase 1
Glutamic--pyruvic transaminase 1
Gene namesi
Name:GPT
Synonyms:AAT1, GPT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000167701.13
HGNCiHGNC:4552 GPT
MIMi138200 gene
neXtProtiNX_P24298

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi2875
OpenTargetsiENSG00000167701
PharmGKBiPA28947

Chemistry databases

ChEMBLiCHEMBL5929
DrugBankiDB00160 L-Alanine
DB00142 L-Glutamic Acid
DB00780 Phenelzine
DB00114 Pyridoxal Phosphate
DB09325 Sodium fluoride

Polymorphism and mutation databases

BioMutaiGPT
DMDMi46577683

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001239332 – 496Alanine aminotransferase 1Add BLAST495

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei22PhosphothreonineCombined sources1
Modified residuei314N6-(pyridoxal phosphate)lysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP24298
MaxQBiP24298
PaxDbiP24298
PeptideAtlasiP24298
PRIDEiP24298
ProteomicsDBi54194

PTM databases

iPTMnetiP24298
PhosphoSitePlusiP24298

Expressioni

Tissue specificityi

Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.1 Publication

Inductioni

By glucocorticoids.

Gene expression databases

BgeeiENSG00000167701 Expressed in 128 organ(s), highest expression level in right lobe of liver
CleanExiHS_GPT
GenevisibleiP24298 HS

Organism-specific databases

HPAiCAB032997
CAB032999
HPA031059
HPA031060

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi109133, 6 interactors
IntActiP24298, 3 interactors
STRINGi9606.ENSP00000378408

Structurei

3D structure databases

ProteinModelPortaliP24298
SMRiP24298
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0258 Eukaryota
COG0436 LUCA
GeneTreeiENSGT00650000093331
HOGENOMiHOG000215020
HOVERGENiHBG026148
InParanoidiP24298
KOiK00814
OMAiFHAKFTL
OrthoDBiEOG091G0PY3
PhylomeDBiP24298
TreeFamiTF300839

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24298-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ
60 70 80 90 100
ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF
110 120 130 140 150
PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD
160 170 180 190 200
PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG
210 220 230 240 250
AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ
260 270 280 290 300
TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY
310 320 330 340 350
AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP
360 370 380 390 400
PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA
410 420 430 440 450
PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC
460 470 480 490
VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS
Length:496
Mass (Da):54,637
Last modified:January 23, 2007 - v3
Checksum:i23705E7A3A4283B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4 – 7STGD → RRGN in BAA01186 (Ref. 7) Curated4
Sequence conflicti39G → S in BAA01186 (Ref. 7) Curated1
Sequence conflicti222H → A AA sequence (PubMed:1931970).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_00056114H → N in allele GPT*2. 2 PublicationsCorresponds to variant dbSNP:rs1063739Ensembl.1
Natural variantiVAR_075711430E → Q Found in patient with Joubert syndrome; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs141505249Ensembl.1
Natural variantiVAR_075712452V → L Found in patient with Joubert syndrome; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs147998249Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70732 Genomic DNA Translation: AAC51155.1
BT006992 mRNA Translation: AAP35638.1
EF444981 Genomic DNA Translation: ACA05996.1
CH471162 Genomic DNA Translation: EAW82077.1
CH471162 Genomic DNA Translation: EAW82078.1
BC018207 mRNA Translation: AAH18207.1
D10355 mRNA Translation: BAA01186.1
CCDSiCCDS6430.1
PIRiA40465
RefSeqiNP_005300.1, NM_005309.2
XP_011515295.1, XM_011516993.2
UniGeneiHs.103502

Genome annotation databases

EnsembliENST00000394955; ENSP00000378408; ENSG00000167701
ENST00000528431; ENSP00000433586; ENSG00000167701
GeneIDi2875
KEGGihsa:2875
UCSCiuc003zdh.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

SHMPD

The Singapore human mutation and polymorphism database

Wikipedia

Alanine transaminase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70732 Genomic DNA Translation: AAC51155.1
BT006992 mRNA Translation: AAP35638.1
EF444981 Genomic DNA Translation: ACA05996.1
CH471162 Genomic DNA Translation: EAW82077.1
CH471162 Genomic DNA Translation: EAW82078.1
BC018207 mRNA Translation: AAH18207.1
D10355 mRNA Translation: BAA01186.1
CCDSiCCDS6430.1
PIRiA40465
RefSeqiNP_005300.1, NM_005309.2
XP_011515295.1, XM_011516993.2
UniGeneiHs.103502

3D structure databases

ProteinModelPortaliP24298
SMRiP24298
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109133, 6 interactors
IntActiP24298, 3 interactors
STRINGi9606.ENSP00000378408

Chemistry databases

ChEMBLiCHEMBL5929
DrugBankiDB00160 L-Alanine
DB00142 L-Glutamic Acid
DB00780 Phenelzine
DB00114 Pyridoxal Phosphate
DB09325 Sodium fluoride

PTM databases

iPTMnetiP24298
PhosphoSitePlusiP24298

Polymorphism and mutation databases

BioMutaiGPT
DMDMi46577683

Proteomic databases

EPDiP24298
MaxQBiP24298
PaxDbiP24298
PeptideAtlasiP24298
PRIDEiP24298
ProteomicsDBi54194

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394955; ENSP00000378408; ENSG00000167701
ENST00000528431; ENSP00000433586; ENSG00000167701
GeneIDi2875
KEGGihsa:2875
UCSCiuc003zdh.5 human

Organism-specific databases

CTDi2875
DisGeNETi2875
EuPathDBiHostDB:ENSG00000167701.13
GeneCardsiGPT
HGNCiHGNC:4552 GPT
HPAiCAB032997
CAB032999
HPA031059
HPA031060
MIMi138200 gene
neXtProtiNX_P24298
OpenTargetsiENSG00000167701
PharmGKBiPA28947
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0258 Eukaryota
COG0436 LUCA
GeneTreeiENSGT00650000093331
HOGENOMiHOG000215020
HOVERGENiHBG026148
InParanoidiP24298
KOiK00814
OMAiFHAKFTL
OrthoDBiEOG091G0PY3
PhylomeDBiP24298
TreeFamiTF300839

Enzyme and pathway databases

UniPathwayi
UPA00528;UER00586

BioCyciMetaCyc:HS09610-MONOMER
ReactomeiR-HSA-70614 Amino acid synthesis and interconversion (transamination)

Miscellaneous databases

GenomeRNAii2875
PROiPR:P24298
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167701 Expressed in 128 organ(s), highest expression level in right lobe of liver
CleanExiHS_GPT
GenevisibleiP24298 HS

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiALAT1_HUMAN
AccessioniPrimary (citable) accession number: P24298
Secondary accession number(s): B0YJ18
, D3DWM7, P78398, Q93076
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 185 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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