UniProtKB - P24288 (BCAT1_MOUSE)
Protein
Branched-chain-amino-acid aminotransferase, cytosolic
Gene
Bcat1
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
Catalytic activityi
Cofactori
GO - Molecular functioni
- branched-chain-amino-acid transaminase activity Source: MGI
- identical protein binding Source: MGI
- L-isoleucine transaminase activity Source: UniProtKB-EC
- L-leucine transaminase activity Source: UniProtKB-EC
- L-valine transaminase activity Source: UniProtKB-EC
GO - Biological processi
- branched-chain amino acid catabolic process Source: MGI
- leucine biosynthetic process Source: GO_Central
- valine biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
Reactomei | R-MMU-70895, Branched-chain amino acid catabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Branched-chain-amino-acid aminotransferase, cytosolic (EC:2.6.1.42)Short name: BCAT(c) Alternative name(s): Protein ECA39 |
Gene namesi | Name:Bcat1 Synonyms:Eca39 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104861, Bcat1 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000103293 | 1 – 386 | Branched-chain-amino-acid aminotransferase, cytosolicAdd BLAST | 386 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 222 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | P24288 |
jPOSTi | P24288 |
PaxDbi | P24288 |
PeptideAtlasi | P24288 |
PRIDEi | P24288 |
PTM databases
iPTMneti | P24288 |
PhosphoSitePlusi | P24288 |
SwissPalmi | P24288 |
Expressioni
Tissue specificityi
Expressed in brain and kidney. Overexpressed in MYC-induced brain tumors, lymphomas, as well as in a teratocarcinoma cell line.
Developmental stagei
Highly expressed at day 9 of embryogenesis. Expression decreases to moderate levels through day 13. In the developing embryo, expressed in the brain, somites and mesenophric tubules.
Gene expression databases
Bgeei | ENSMUSG00000030268, Expressed in cumulus cell and 287 other tissues |
ExpressionAtlasi | P24288, baseline and differential |
Genevisiblei | P24288, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198311, 7 interactors |
IntActi | P24288, 1 interactor |
MINTi | P24288 |
STRINGi | 10090.ENSMUSP00000032402 |
Miscellaneous databases
RNActi | P24288, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | KOG0975, Eukaryota |
GeneTreei | ENSGT00390000009532 |
HOGENOMi | CLU_031922_0_3_1 |
InParanoidi | P24288 |
OMAi | LTEVFAC |
Family and domain databases
CDDi | cd01557, BCAT_beta_family, 1 hit |
Gene3Di | 3.20.10.10, 1 hit 3.30.470.10, 1 hit |
InterProi | View protein in InterPro IPR001544, Aminotrans_IV IPR018300, Aminotrans_IV_CS IPR036038, Aminotransferase-like IPR005786, B_amino_transII IPR043132, BCAT-like_C IPR043131, BCAT-like_N IPR033939, BCAT_family |
Pfami | View protein in Pfam PF01063, Aminotran_4, 1 hit |
PIRSFi | PIRSF006468, BCAT1, 1 hit |
SUPFAMi | SSF56752, SSF56752, 1 hit |
TIGRFAMsi | TIGR01123, ilvE_II, 1 hit |
PROSITEi | View protein in PROSITE PS00770, AA_TRANSFER_CLASS_4, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
P24288-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKDCSNGCSA PFAGERGSEE VAETFRAKDL IITPATVLKE KPDPDSLVFG
60 70 80 90 100
ATFTDHMLTV EWSSASGWEK PHIKPFGNLP IHPAASVLHY AVELFEGLKA
110 120 130 140 150
FRGVDNKIRL FRPDLNMDRM CRSAVRTTLP MFDKEELLKC ILQLLQIDQE
160 170 180 190 200
WVPYSTSASL YIRPTFIGTE PSLGVKKPSK ALLFVILSPV GPYFSSGSFT
210 220 230 240 250
PVSLWANPKY IRAWKGGTGD CKMGGNYGAS LLAQCEAVEN GCQQVLWLYG
260 270 280 290 300
KDNQITEVGT MNLFLYWINE DGEEELATPP LDGIILPGVT RQSILELAQQ
310 320 330 340 350
WGEFKVCERH LTMDDLATAL EGNRVKEMFG SGTACVVCPV SDILYKGQML
360 370 380
HIPTMENGPK LASRILGKLT DIQYGRVESD WTIELP
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q4K3 | E9Q4K3_MOUSE | Branched-chain-amino-acid aminotran... | Bcat1 | 393 | Annotation score: | ||
Q8CBC8 | Q8CBC8_MOUSE | Branched-chain-amino-acid aminotran... | Bcat1 | 453 | Annotation score: | ||
A0A0N4SV61 | A0A0N4SV61_MOUSE | Branched-chain-amino-acid aminotran... | Bcat1 | 180 | Annotation score: | ||
B2KFA8 | B2KFA8_MOUSE | Branched-chain-amino-acid aminotran... | Bcat1 | 223 | Annotation score: | ||
B2KFA9 | B2KFA9_MOUSE | Branched-chain-amino-acid aminotran... | Bcat1 | 136 | Annotation score: | ||
F6YUH3 | F6YUH3_MOUSE | Branched-chain-amino-acid aminotran... | Bcat1 | 83 | Annotation score: |
Sequence cautioni
The sequence CAA35543 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 238 | V → A in AAH53706 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 265 | L → P in AAH53706 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U42443 mRNA Translation: AAB05673.1 X17502 mRNA Translation: CAA35543.1 Frameshift. BC053706 mRNA Translation: AAH53706.1 |
CCDSi | CCDS39703.1 |
PIRi | S13108 |
RefSeqi | NP_031558.3, NM_007532.5 |
Genome annotation databases
Ensembli | ENSMUST00000111742; ENSMUSP00000107371; ENSMUSG00000030268 |
GeneIDi | 12035 |
KEGGi | mmu:12035 |
UCSCi | uc009eqt.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U42443 mRNA Translation: AAB05673.1 X17502 mRNA Translation: CAA35543.1 Frameshift. BC053706 mRNA Translation: AAH53706.1 |
CCDSi | CCDS39703.1 |
PIRi | S13108 |
RefSeqi | NP_031558.3, NM_007532.5 |
3D structure databases
SMRi | P24288 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198311, 7 interactors |
IntActi | P24288, 1 interactor |
MINTi | P24288 |
STRINGi | 10090.ENSMUSP00000032402 |
PTM databases
iPTMneti | P24288 |
PhosphoSitePlusi | P24288 |
SwissPalmi | P24288 |
Proteomic databases
EPDi | P24288 |
jPOSTi | P24288 |
PaxDbi | P24288 |
PeptideAtlasi | P24288 |
PRIDEi | P24288 |
Protocols and materials databases
Antibodypediai | 24197, 349 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000111742; ENSMUSP00000107371; ENSMUSG00000030268 |
GeneIDi | 12035 |
KEGGi | mmu:12035 |
UCSCi | uc009eqt.2, mouse |
Organism-specific databases
CTDi | 586 |
MGIi | MGI:104861, Bcat1 |
Phylogenomic databases
eggNOGi | KOG0975, Eukaryota |
GeneTreei | ENSGT00390000009532 |
HOGENOMi | CLU_031922_0_3_1 |
InParanoidi | P24288 |
OMAi | LTEVFAC |
Enzyme and pathway databases
Reactomei | R-MMU-70895, Branched-chain amino acid catabolism |
Miscellaneous databases
BioGRID-ORCSi | 12035, 2 hits in 17 CRISPR screens |
ChiTaRSi | Bcat1, mouse |
PROi | PR:P24288 |
RNActi | P24288, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030268, Expressed in cumulus cell and 287 other tissues |
ExpressionAtlasi | P24288, baseline and differential |
Genevisiblei | P24288, MM |
Family and domain databases
CDDi | cd01557, BCAT_beta_family, 1 hit |
Gene3Di | 3.20.10.10, 1 hit 3.30.470.10, 1 hit |
InterProi | View protein in InterPro IPR001544, Aminotrans_IV IPR018300, Aminotrans_IV_CS IPR036038, Aminotransferase-like IPR005786, B_amino_transII IPR043132, BCAT-like_C IPR043131, BCAT-like_N IPR033939, BCAT_family |
Pfami | View protein in Pfam PF01063, Aminotran_4, 1 hit |
PIRSFi | PIRSF006468, BCAT1, 1 hit |
SUPFAMi | SSF56752, SSF56752, 1 hit |
TIGRFAMsi | TIGR01123, ilvE_II, 1 hit |
PROSITEi | View protein in PROSITE PS00770, AA_TRANSFER_CLASS_4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BCAT1_MOUSE | |
Accessioni | P24288Primary (citable) accession number: P24288 Secondary accession number(s): Q7TMT2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families