UniProtKB - P24255 (RP54_ECOLI)
Protein
RNA polymerase sigma-54 factor
Gene
rpoN
Organism
Escherichia coli (strain K12)
Status
Functioni
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma-54 and core RNA polymerase) serves as the receptor for the receipt of the melting signal from the remotely bound activator protein GlnG(NtrC).
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 366 – 385 | H-T-H motifBy similarityAdd BLAST | 20 |
GO - Molecular functioni
- DNA-binding transcription activator activity Source: CollecTF
- DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- sigma factor activity Source: UniProtKB-KW
- transcription regulatory region sequence-specific DNA binding Source: CollecTF
GO - Biological processi
- arginine metabolic process Source: UniProtKB-KW
- DNA-templated transcription, initiation Source: InterPro
Keywordsi
Molecular function | DNA-binding, Nucleotidyltransferase, Sigma factor, Transferase |
Biological process | Arginine metabolism, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:RPON-MONOMER MetaCyc:RPON-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: RNA polymerase sigma-54 factor |
Gene namesi | Name:rpoN Synonyms:glnF, ntrA Ordered Locus Names:b3202, JW3169 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- protein-DNA complex Source: CollecTF
Keywords - Cellular componenti
DNA-directed RNA polymerasePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205529 | 1 – 477 | RNA polymerase sigma-54 factorAdd BLAST | 477 |
Proteomic databases
jPOSTi | P24255 |
PaxDbi | P24255 |
PRIDEi | P24255 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259282, 32 interactors |
ComplexPortali | CPX-4884, DNA-directed RNA polymerase holoenzyme complex, Sigma54 variant |
DIPi | DIP-10776N |
IntActi | P24255, 14 interactors |
STRINGi | 511145.b3202 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P24255 |
SMRi | P24255 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 454 – 462 | RPON box | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 3 – 39 | Gln-richAdd BLAST | 37 |
Sequence similaritiesi
Belongs to the sigma-54 factor family.Curated
Phylogenomic databases
eggNOGi | COG1508, Bacteria |
HOGENOMi | CLU_020569_0_1_6 |
InParanoidi | P24255 |
PhylomeDBi | P24255 |
Family and domain databases
DisProti | DP01169 |
Gene3Di | 1.10.10.1330, 1 hit |
InterProi | View protein in InterPro IPR000394, RNA_pol_sigma_54 IPR007046, RNA_pol_sigma_54_core-bd IPR007634, RNA_pol_sigma_54_DNA-bd IPR038709, RpoN_core-bd_sf |
PANTHERi | PTHR32248, PTHR32248, 1 hit |
Pfami | View protein in Pfam PF00309, Sigma54_AID, 1 hit PF04963, Sigma54_CBD, 1 hit PF04552, Sigma54_DBD, 1 hit |
PIRSFi | PIRSF000774, RpoN, 1 hit |
PRINTSi | PR00045, SIGMA54FCT |
TIGRFAMsi | TIGR02395, rpoN_sigma, 1 hit |
PROSITEi | View protein in PROSITE PS00717, SIGMA54_1, 1 hit PS00718, SIGMA54_2, 1 hit PS50044, SIGMA54_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P24255-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKQGLQLRLS QQLAMTPQLQ QAIRLLQLST LELQQELQQA LESNPLLEQI
60 70 80 90 100
DTHEEIDTRE TQDSETLDTA DALEQKEMPE ELPLDASWDT IYTAGTPSGT
110 120 130 140 150
SGDYIDDELP VYQGETTQTL QDYLMWQVEL TPFSDTDRAI ATSIVDAVDE
160 170 180 190 200
TGYLTVPLED ILESIGDEEI DIDEVEAVLK RIQRFDPVGV AAKDLRDCLL
210 220 230 240 250
IQLSQFDKTT PWLEEARLII SDHLDLLANH DFRTLMRVTR LKEDVLKEAV
260 270 280 290 300
NLIQSLDPRP GQSIQTGEPE YVIPDVLVRK HNGHWTVELN SDSIPRLQIN
310 320 330 340 350
QHYASMCNNA RNDGDSQFIR SNLQDAKWLI KSLESRNDTL LRVSRCIVEQ
360 370 380 390 400
QQAFFEQGEE YMKPMVLADI AQAVEMHEST ISRVTTQKYL HSPRGIFELK
410 420 430 440 450
YFFSSHVNTE GGGEASSTAI RALVKKLIAA ENPAKPLSDS KLTSLLSEQG
460 470
IMVARRTVAK YRESLSIPPS NQRKQLV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 99 – 103 | GTSGD → APAVT (PubMed:2203540).Curated | 5 | |
Sequence conflicti | 99 – 103 | GTSGD → APAVT (PubMed:8444818).Curated | 5 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M57612 Genomic DNA Translation: AAA62790.1 D12938 Genomic DNA Translation: BAA02315.1 Z27094 Genomic DNA Translation: CAA81617.1 U12684 Genomic DNA Translation: AAB60163.1 U18997 Genomic DNA Translation: AAA58004.1 U00096 Genomic DNA Translation: AAC76234.1 AP009048 Genomic DNA Translation: BAE77246.1 |
PIRi | I57054, A35695 |
RefSeqi | NP_417669.1, NC_000913.3 WP_000809057.1, NZ_SSZK01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC76234; AAC76234; b3202 BAE77246; BAE77246; BAE77246 |
GeneIDi | 947714 |
KEGGi | ecj:JW3169 eco:b3202 |
PATRICi | fig|1411691.4.peg.3529 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M57612 Genomic DNA Translation: AAA62790.1 D12938 Genomic DNA Translation: BAA02315.1 Z27094 Genomic DNA Translation: CAA81617.1 U12684 Genomic DNA Translation: AAB60163.1 U18997 Genomic DNA Translation: AAA58004.1 U00096 Genomic DNA Translation: AAC76234.1 AP009048 Genomic DNA Translation: BAE77246.1 |
PIRi | I57054, A35695 |
RefSeqi | NP_417669.1, NC_000913.3 WP_000809057.1, NZ_SSZK01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MT3 | NMR | - | A | 414-477 | [»] | |
BMRBi | P24255 | |||||
SMRi | P24255 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259282, 32 interactors |
ComplexPortali | CPX-4884, DNA-directed RNA polymerase holoenzyme complex, Sigma54 variant |
DIPi | DIP-10776N |
IntActi | P24255, 14 interactors |
STRINGi | 511145.b3202 |
Proteomic databases
jPOSTi | P24255 |
PaxDbi | P24255 |
PRIDEi | P24255 |
Genome annotation databases
EnsemblBacteriai | AAC76234; AAC76234; b3202 BAE77246; BAE77246; BAE77246 |
GeneIDi | 947714 |
KEGGi | ecj:JW3169 eco:b3202 |
PATRICi | fig|1411691.4.peg.3529 |
Organism-specific databases
EchoBASEi | EB0891 |
Phylogenomic databases
eggNOGi | COG1508, Bacteria |
HOGENOMi | CLU_020569_0_1_6 |
InParanoidi | P24255 |
PhylomeDBi | P24255 |
Enzyme and pathway databases
BioCyci | EcoCyc:RPON-MONOMER MetaCyc:RPON-MONOMER |
Miscellaneous databases
PROi | PR:P24255 |
Gene expression databases
CollecTFi | EXPREG_00000990 |
Family and domain databases
DisProti | DP01169 |
Gene3Di | 1.10.10.1330, 1 hit |
InterProi | View protein in InterPro IPR000394, RNA_pol_sigma_54 IPR007046, RNA_pol_sigma_54_core-bd IPR007634, RNA_pol_sigma_54_DNA-bd IPR038709, RpoN_core-bd_sf |
PANTHERi | PTHR32248, PTHR32248, 1 hit |
Pfami | View protein in Pfam PF00309, Sigma54_AID, 1 hit PF04963, Sigma54_CBD, 1 hit PF04552, Sigma54_DBD, 1 hit |
PIRSFi | PIRSF000774, RpoN, 1 hit |
PRINTSi | PR00045, SIGMA54FCT |
TIGRFAMsi | TIGR02395, rpoN_sigma, 1 hit |
PROSITEi | View protein in PROSITE PS00717, SIGMA54_1, 1 hit PS00718, SIGMA54_2, 1 hit PS50044, SIGMA54_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RP54_ECOLI | |
Accessioni | P24255Primary (citable) accession number: P24255 Secondary accession number(s): Q2M910 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | February 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families