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Protein

RNA polymerase sigma-54 factor

Gene

rpoN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma-54 and core RNA polymerase) serves as the receptor for the receipt of the melting signal from the remotely bound activator protein GlnG(NtrC).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi366 – 385H-T-H motifBy similarityAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Sigma factor, Transferase
Biological processArginine metabolism, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:RPON-MONOMER
MetaCyc:RPON-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma-54 factor
Gene namesi
Name:rpoN
Synonyms:glnF, ntrA
Ordered Locus Names:b3202, JW3169
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10898 rpoN

Subcellular locationi

GO - Cellular componenti

  • protein-DNA complex Source: CollecTF

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002055291 – 477RNA polymerase sigma-54 factorAdd BLAST477

Proteomic databases

PaxDbiP24255
PRIDEiP24255

Expressioni

Gene expression databases

CollecTFiEXPREG_00000990

Interactioni

Protein-protein interaction databases

BioGridi4259282, 32 interactors
DIPiDIP-10776N
IntActiP24255, 14 interactors
STRINGi316385.ECDH10B_3376

Structurei

Secondary structure

1477
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi416 – 419Combined sources4
Turni420 – 422Combined sources3
Helixi423 – 429Combined sources7
Helixi441 – 447Combined sources7
Turni448 – 450Combined sources3
Helixi454 – 465Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MT3NMR-A414-477[»]
ProteinModelPortaliP24255
SMRiP24255
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi454 – 462RPON box9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 39Gln-richAdd BLAST37

Sequence similaritiesi

Belongs to the sigma-54 factor family.Curated

Phylogenomic databases

eggNOGiENOG4105DTW Bacteria
COG1508 LUCA
HOGENOMiHOG000071905
InParanoidiP24255
KOiK03092
OMAiVTTQKFM
PhylomeDBiP24255

Family and domain databases

Gene3Di1.10.10.1330, 1 hit
InterProiView protein in InterPro
IPR000394 RNA_pol_sigma_54
IPR007046 RNA_pol_sigma_54_core-bd
IPR007634 RNA_pol_sigma_54_DNA-bd
IPR038709 RpoN_core-bd_sf
PANTHERiPTHR32248 PTHR32248, 1 hit
PfamiView protein in Pfam
PF00309 Sigma54_AID, 1 hit
PF04963 Sigma54_CBD, 1 hit
PF04552 Sigma54_DBD, 1 hit
PIRSFiPIRSF000774 RpoN, 1 hit
PRINTSiPR00045 SIGMA54FCT
TIGRFAMsiTIGR02395 rpoN_sigma, 1 hit
PROSITEiView protein in PROSITE
PS00717 SIGMA54_1, 1 hit
PS00718 SIGMA54_2, 1 hit
PS50044 SIGMA54_3, 1 hit

Sequencei

Sequence statusi: Complete.

P24255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQGLQLRLS QQLAMTPQLQ QAIRLLQLST LELQQELQQA LESNPLLEQI
60 70 80 90 100
DTHEEIDTRE TQDSETLDTA DALEQKEMPE ELPLDASWDT IYTAGTPSGT
110 120 130 140 150
SGDYIDDELP VYQGETTQTL QDYLMWQVEL TPFSDTDRAI ATSIVDAVDE
160 170 180 190 200
TGYLTVPLED ILESIGDEEI DIDEVEAVLK RIQRFDPVGV AAKDLRDCLL
210 220 230 240 250
IQLSQFDKTT PWLEEARLII SDHLDLLANH DFRTLMRVTR LKEDVLKEAV
260 270 280 290 300
NLIQSLDPRP GQSIQTGEPE YVIPDVLVRK HNGHWTVELN SDSIPRLQIN
310 320 330 340 350
QHYASMCNNA RNDGDSQFIR SNLQDAKWLI KSLESRNDTL LRVSRCIVEQ
360 370 380 390 400
QQAFFEQGEE YMKPMVLADI AQAVEMHEST ISRVTTQKYL HSPRGIFELK
410 420 430 440 450
YFFSSHVNTE GGGEASSTAI RALVKKLIAA ENPAKPLSDS KLTSLLSEQG
460 470
IMVARRTVAK YRESLSIPPS NQRKQLV
Length:477
Mass (Da):53,990
Last modified:February 1, 1994 - v2
Checksum:i1FAA4273A02B34FD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99 – 103GTSGD → APAVT (PubMed:2203540).Curated5
Sequence conflicti99 – 103GTSGD → APAVT (PubMed:8444818).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57612 Genomic DNA Translation: AAA62790.1
D12938 Genomic DNA Translation: BAA02315.1
Z27094 Genomic DNA Translation: CAA81617.1
U12684 Genomic DNA Translation: AAB60163.1
U18997 Genomic DNA Translation: AAA58004.1
U00096 Genomic DNA Translation: AAC76234.1
AP009048 Genomic DNA Translation: BAE77246.1
PIRiI57054 A35695
RefSeqiNP_417669.1, NC_000913.3
WP_000809057.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76234; AAC76234; b3202
BAE77246; BAE77246; BAE77246
GeneIDi947714
KEGGiecj:JW3169
eco:b3202
PATRICifig|1411691.4.peg.3529

Similar proteinsi

Entry informationi

Entry nameiRP54_ECOLI
AccessioniPrimary (citable) accession number: P24255
Secondary accession number(s): Q2M910
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 1, 1994
Last modified: June 20, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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