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UniProtKB - P24255 (RP54_ECOLI)

Entry version 154 (07 Oct 2020)
Sequence version 2 (01 Feb 1994)
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Protein

RNA polymerase sigma-54 factor

Gene

rpoN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma-54 and core RNA polymerase) serves as the receptor for the receipt of the melting signal from the remotely bound activator protein GlnG(NtrC).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position and type of each DNA-binding domain present within the protein.<p><a href='/help/dna_bind' target='_top'>More...</a></p>DNA bindingi366 – 385H-T-H motifBy similarityAdd BLAST20

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Sigma factor, Transferase
Biological processArginine metabolism, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:RPON-MONOMER
MetaCyc:RPON-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
RNA polymerase sigma-54 factor
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:rpoN
Synonyms:glnF, ntrA
Ordered Locus Names:b3202, JW3169
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Complete GO annotation on QuickGO ...

Keywords - Cellular componenti

DNA-directed RNA polymerase

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002055291 – 477RNA polymerase sigma-54 factorAdd BLAST477

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P24255

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P24255

PRoteomics IDEntifications database

More...PRIDEi		P24255

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

CollecTF database of bacterial transcription factor binding sites

More...CollecTFi		EXPREG_00000990

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4259282, 32 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-4884, DNA-directed RNA polymerase holoenzyme complex, Sigma54 variant

Database of interacting proteins

More...DIPi		DIP-10776N

Protein interaction database and analysis system

More...IntActi		P24255, 14 interactors

STRING: functional protein association networks

More...STRINGi		511145.b3202

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1477
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Biological Magnetic Resonance Data Bank

More...BMRBi		P24255

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P24255

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi454 – 462RPON box9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi3 – 39Gln-richAdd BLAST37

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the sigma-54 factor family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG1508, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_020569_0_1_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P24255

KEGG Orthology (KO)

More...KOi		K03092

Database for complete collections of gene phylogenies

More...PhylomeDBi		P24255

Family and domain databases

Database of protein disorder

More...DisProti		DP01169

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.10.1330, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR000394, RNA_pol_sigma_54
IPR007046, RNA_pol_sigma_54_core-bd
IPR007634, RNA_pol_sigma_54_DNA-bd
IPR038709, RpoN_core-bd_sf

The PANTHER Classification System

More...PANTHERi		PTHR32248, PTHR32248, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00309, Sigma54_AID, 1 hit
PF04963, Sigma54_CBD, 1 hit
PF04552, Sigma54_DBD, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF000774, RpoN, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00045, SIGMA54FCT

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR02395, rpoN_sigma, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00717, SIGMA54_1, 1 hit
PS00718, SIGMA54_2, 1 hit
PS50044, SIGMA54_3, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P24255-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKQGLQLRLS QQLAMTPQLQ QAIRLLQLST LELQQELQQA LESNPLLEQI 
        60         70         80         90        100
DTHEEIDTRE TQDSETLDTA DALEQKEMPE ELPLDASWDT IYTAGTPSGT 
       110        120        130        140        150
SGDYIDDELP VYQGETTQTL QDYLMWQVEL TPFSDTDRAI ATSIVDAVDE 
       160        170        180        190        200
TGYLTVPLED ILESIGDEEI DIDEVEAVLK RIQRFDPVGV AAKDLRDCLL 
       210        220        230        240        250
IQLSQFDKTT PWLEEARLII SDHLDLLANH DFRTLMRVTR LKEDVLKEAV 
       260        270        280        290        300
NLIQSLDPRP GQSIQTGEPE YVIPDVLVRK HNGHWTVELN SDSIPRLQIN 
       310        320        330        340        350
QHYASMCNNA RNDGDSQFIR SNLQDAKWLI KSLESRNDTL LRVSRCIVEQ 
       360        370        380        390        400
QQAFFEQGEE YMKPMVLADI AQAVEMHEST ISRVTTQKYL HSPRGIFELK 
       410        420        430        440        450
YFFSSHVNTE GGGEASSTAI RALVKKLIAA ENPAKPLSDS KLTSLLSEQG 
       460        470 
IMVARRTVAK YRESLSIPPS NQRKQLV
Length:477
Mass (Da):53,990
Last modified:February 1, 1994 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i1FAA4273A02B34FD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti99 – 103GTSGD → APAVT (PubMed:2203540).Curated5
Sequence conflicti99 – 103GTSGD → APAVT (PubMed:8444818).Curated5

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
M57612 Genomic DNA Translation: AAA62790.1
D12938 Genomic DNA Translation: BAA02315.1
Z27094 Genomic DNA Translation: CAA81617.1
U12684 Genomic DNA Translation: AAB60163.1
U18997 Genomic DNA Translation: AAA58004.1
U00096 Genomic DNA Translation: AAC76234.1
AP009048 Genomic DNA Translation: BAE77246.1

Protein sequence database of the Protein Information Resource

More...PIRi		I57054, A35695

NCBI Reference Sequences

More...RefSeqi		NP_417669.1, NC_000913.3
WP_000809057.1, NZ_SSZK01000007.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC76234; AAC76234; b3202
BAE77246; BAE77246; BAE77246

Database of genes from NCBI RefSeq genomes

More...GeneIDi		947714

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW3169
eco:b3202

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.3529

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57612 Genomic DNA Translation: AAA62790.1
D12938 Genomic DNA Translation: BAA02315.1
Z27094 Genomic DNA Translation: CAA81617.1
U12684 Genomic DNA Translation: AAB60163.1
U18997 Genomic DNA Translation: AAA58004.1
U00096 Genomic DNA Translation: AAC76234.1
AP009048 Genomic DNA Translation: BAE77246.1
PIRiI57054, A35695
RefSeqiNP_417669.1, NC_000913.3
WP_000809057.1, NZ_SSZK01000007.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MT3NMR-A414-477[»]
BMRBiP24255
SMRiP24255
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4259282, 32 interactors
ComplexPortaliCPX-4884, DNA-directed RNA polymerase holoenzyme complex, Sigma54 variant
DIPiDIP-10776N
IntActiP24255, 14 interactors
STRINGi511145.b3202

Proteomic databases

jPOSTiP24255
PaxDbiP24255
PRIDEiP24255

Genome annotation databases

EnsemblBacteriaiAAC76234; AAC76234; b3202
BAE77246; BAE77246; BAE77246
GeneIDi947714
KEGGiecj:JW3169
eco:b3202
PATRICifig|1411691.4.peg.3529

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB0891

Phylogenomic databases

eggNOGiCOG1508, Bacteria
HOGENOMiCLU_020569_0_1_6
InParanoidiP24255
KOiK03092
PhylomeDBiP24255

Enzyme and pathway databases

BioCyciEcoCyc:RPON-MONOMER
MetaCyc:RPON-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P24255

Gene expression databases

CollecTFiEXPREG_00000990

Family and domain databases

DisProtiDP01169
Gene3Di1.10.10.1330, 1 hit
InterProiView protein in InterPro
IPR000394, RNA_pol_sigma_54
IPR007046, RNA_pol_sigma_54_core-bd
IPR007634, RNA_pol_sigma_54_DNA-bd
IPR038709, RpoN_core-bd_sf
PANTHERiPTHR32248, PTHR32248, 1 hit
PfamiView protein in Pfam
PF00309, Sigma54_AID, 1 hit
PF04963, Sigma54_CBD, 1 hit
PF04552, Sigma54_DBD, 1 hit
PIRSFiPIRSF000774, RpoN, 1 hit
PRINTSiPR00045, SIGMA54FCT
TIGRFAMsiTIGR02395, rpoN_sigma, 1 hit
PROSITEiView protein in PROSITE
PS00717, SIGMA54_1, 1 hit
PS00718, SIGMA54_2, 1 hit
PS50044, SIGMA54_3, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRP54_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P24255
Secondary accession number(s): Q2M910
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 1, 1994
Last modified: October 7, 2020
This is version 154 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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