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Protein

ATP-dependent DNA helicase RecG

Gene

recG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Play a critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA). Has a role in constitutive stable DNA replication (cSDR) and R-loop formation. Is genetically synergistic to RadA and RuvABC (PubMed:12446634, PubMed:25484163).4 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi296 – 303ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent DNA helicase activity Source: EcoCyc
  • DNA binding Source: UniProtKB-KW

GO - Biological processi

  • DNA duplex unwinding Source: EcoCyc
  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • response to radiation Source: EcoCyc

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10829-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase RecG (EC:3.6.4.12)
Gene namesi
Name:recG
Synonyms:radC
Ordered Locus Names:b3652, JW3627
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10829 recG

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

Very slight sensitivity to ciprofloxacin (CFX), UV and azidothymidine (AZT) in single deletion mutants, but a radA-recG deletion is extremely sensitive to CFX and AZT, less so to UV (PubMed:12446634, PubMed:25484163). The SOS response is induced in this double mutant, indicating spontaneous DNA damage. AZT sensitivity is suppressed by further recA or recF deletions, suggesting AZT-induced DNA gaps are processed into lethal intermediates in a RecA-dependent fashion mediated by RecF (PubMed:25484163).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi411 – 693Missing in radC102; causes mild UV and X-ray sensitivity. 1 PublicationAdd BLAST283

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001021401 – 693ATP-dependent DNA helicase RecGAdd BLAST693

Proteomic databases

PaxDbiP24230
PRIDEiP24230

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
aceFP069592EBI-556882,EBI-542707

Protein-protein interaction databases

BioGridi4262929, 148 interactors
DIPiDIP-10653N
IntActiP24230, 30 interactors
STRINGi316385.ECDH10B_3834

Structurei

3D structure databases

ProteinModelPortaliP24230
SMRiP24230
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini283 – 448Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini482 – 628Helicase C-terminalPROSITE-ProRule annotationAdd BLAST147

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi397 – 400DEQH box4

Sequence similaritiesi

Belongs to the helicase family. RecG subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CB5 Bacteria
COG1200 LUCA
HOGENOMiHOG000036617
InParanoidiP24230
KOiK03655
PhylomeDBiP24230

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR004609 ATP-dep_DNA_helicase_RecG
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
IPR033454 RecG_wedge
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF17191 RecG_wedge, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00643 recG, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

P24230-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKGRLLDAVP LSSLTGVGAA LSNKLAKINL HTVQDLLLHL PLRYEDRTHL
60 70 80 90 100
YPIGELLPGV YATVEGEVLN CNISFGGRRM MTCQISDGSG ILTMRFFNFS
110 120 130 140 150
AAMKNSLAAG RRVLAYGEAK RGKYGAEMIH PEYRVQGDLS TPELQETLTP
160 170 180 190 200
VYPTTEGVKQ ATLRKLTDQA LDLLDTCAIE ELLPPELSQG MMTLPEALRT
210 220 230 240 250
LHRPPPTLQL SDLETGQHPA QRRLILEELL AHNLSMLALR AGAQRFHAQP
260 270 280 290 300
LSANDTLKNK LLAALPFKPT GAQARVVAEI ERDMALDVPM MRLVQGDVGS
310 320 330 340 350
GKTLVAALAA LRAIAHGKQV ALMAPTELLA EQHANNFRNW FAPLGIEVGW
360 370 380 390 400
LAGKQKGKAR LAQQEAIASG QVQMIVGTHA IFQEQVQFNG LALVIIDEQH
410 420 430 440 450
RFGVHQRLAL WEKGQQQGFH PHQLIMTATP IPRTLAMTAY ADLDTSVIDE
460 470 480 490 500
LPPGRTPVTT VAIPDTRRTD IIDRVHHACI TEGRQAYWVC TLIEESELLE
510 520 530 540 550
AQAAEATWEE LKLALPELNV GLVHGRMKPA EKQAVMASFK QGELHLLVAT
560 570 580 590 600
TVIEVGVDVP NASLMIIENP ERLGLAQLHQ LRGRVGRGAV ASHCVLLYKT
610 620 630 640 650
PLSKTAQIRL QVLRDSNDGF VIAQKDLEIR GPGELLGTRQ TGNAEFKVAD
660 670 680 690
LLRDQAMIPE VQRLARHIHE RYPQQAKALI ERWMPETERY SNA
Length:693
Mass (Da):76,430
Last modified:March 1, 1992 - v1
Checksum:i7826143A8F4292A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59550 Genomic DNA Translation: CAA42123.1
M64367 Genomic DNA Translation: AAA24513.1
L10328 Genomic DNA Translation: AAA62005.1
U00096 Genomic DNA Translation: AAC76676.1
AP009048 Genomic DNA Translation: BAE77641.1
PIRiJH0265
RefSeqiNP_418109.1, NC_000913.3
WP_000678419.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76676; AAC76676; b3652
BAE77641; BAE77641; BAE77641
GeneIDi948162
KEGGiecj:JW3627
eco:b3652
PATRICifig|511145.12.peg.3772

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59550 Genomic DNA Translation: CAA42123.1
M64367 Genomic DNA Translation: AAA24513.1
L10328 Genomic DNA Translation: AAA62005.1
U00096 Genomic DNA Translation: AAC76676.1
AP009048 Genomic DNA Translation: BAE77641.1
PIRiJH0265
RefSeqiNP_418109.1, NC_000913.3
WP_000678419.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP24230
SMRiP24230
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262929, 148 interactors
DIPiDIP-10653N
IntActiP24230, 30 interactors
STRINGi316385.ECDH10B_3834

Proteomic databases

PaxDbiP24230
PRIDEiP24230

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76676; AAC76676; b3652
BAE77641; BAE77641; BAE77641
GeneIDi948162
KEGGiecj:JW3627
eco:b3652
PATRICifig|511145.12.peg.3772

Organism-specific databases

EchoBASEiEB0822
EcoGeneiEG10829 recG

Phylogenomic databases

eggNOGiENOG4105CB5 Bacteria
COG1200 LUCA
HOGENOMiHOG000036617
InParanoidiP24230
KOiK03655
PhylomeDBiP24230

Enzyme and pathway databases

BioCyciEcoCyc:EG10829-MONOMER

Miscellaneous databases

PROiPR:P24230

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR004609 ATP-dep_DNA_helicase_RecG
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
IPR033454 RecG_wedge
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF17191 RecG_wedge, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00643 recG, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRECG_ECOLI
AccessioniPrimary (citable) accession number: P24230
Secondary accession number(s): P76721, Q2M7W5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 7, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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