UniProtKB - P24230 (RECG_ECOLI)
Protein
ATP-dependent DNA helicase RecG
Gene
recG
Organism
Escherichia coli (strain K12)
Status
Functioni
Plays a critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA). Has a role in constitutive stable DNA replication (cSDR) and R-loop formation. Is genetically synergistic to RadA and RuvABC (PubMed:12446634, PubMed:25484163).4 Publications
Catalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 296 – 303 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- DNA helicase activity Source: EcoCyc
GO - Biological processi
- DNA duplex unwinding Source: EcoCyc
- DNA recombination Source: UniProtKB-KW
- DNA repair Source: GO_Central
- response to radiation Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA damage, DNA recombination, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10829-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:recG Synonyms:radC Ordered Locus Names:b3652, JW3627 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: EcoCyc
Other locations
- Holliday junction helicase complex Source: EcoCyc
Pathology & Biotechi
Disruption phenotypei
Very slight sensitivity to ciprofloxacin (CFX), UV and azidothymidine (AZT) in single deletion mutants, but a radA-recG deletion is extremely sensitive to CFX and AZT, less so to UV (PubMed:12446634, PubMed:25484163). The SOS response is induced in this double mutant, indicating spontaneous DNA damage. AZT sensitivity is suppressed by further recA or recF deletions, suggesting AZT-induced DNA gaps are processed into lethal intermediates in a RecA-dependent fashion mediated by RecF (PubMed:25484163).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 411 – 693 | Missing in radC102; causes mild UV and X-ray sensitivity. 1 PublicationAdd BLAST | 283 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000102140 | 1 – 693 | ATP-dependent DNA helicase RecGAdd BLAST | 693 |
Proteomic databases
jPOSTi | P24230 |
PaxDbi | P24230 |
PRIDEi | P24230 |
Interactioni
Binary interactionsi
P24230
With | #Exp. | IntAct |
---|---|---|
aceF [P06959] | 2 | EBI-556882,EBI-542707 |
Protein-protein interaction databases
BioGRIDi | 4262929, 148 interactors |
DIPi | DIP-10653N |
IntActi | P24230, 30 interactors |
STRINGi | 511145.b3652 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 283 – 448 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 166 | |
Domaini | 482 – 628 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 147 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 397 – 400 | DEQH box | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1200, Bacteria |
HOGENOMi | CLU_005122_7_1_6 |
InParanoidi | P24230 |
PhylomeDBi | P24230 |
Family and domain databases
InterProi | View protein in InterPro IPR004609, ATP-dep_DNA_helicase_RecG IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase IPR033454, RecG_wedge |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit PF17191, RecG_wedge, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR00643, recG, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
P24230-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKGRLLDAVP LSSLTGVGAA LSNKLAKINL HTVQDLLLHL PLRYEDRTHL
60 70 80 90 100
YPIGELLPGV YATVEGEVLN CNISFGGRRM MTCQISDGSG ILTMRFFNFS
110 120 130 140 150
AAMKNSLAAG RRVLAYGEAK RGKYGAEMIH PEYRVQGDLS TPELQETLTP
160 170 180 190 200
VYPTTEGVKQ ATLRKLTDQA LDLLDTCAIE ELLPPELSQG MMTLPEALRT
210 220 230 240 250
LHRPPPTLQL SDLETGQHPA QRRLILEELL AHNLSMLALR AGAQRFHAQP
260 270 280 290 300
LSANDTLKNK LLAALPFKPT GAQARVVAEI ERDMALDVPM MRLVQGDVGS
310 320 330 340 350
GKTLVAALAA LRAIAHGKQV ALMAPTELLA EQHANNFRNW FAPLGIEVGW
360 370 380 390 400
LAGKQKGKAR LAQQEAIASG QVQMIVGTHA IFQEQVQFNG LALVIIDEQH
410 420 430 440 450
RFGVHQRLAL WEKGQQQGFH PHQLIMTATP IPRTLAMTAY ADLDTSVIDE
460 470 480 490 500
LPPGRTPVTT VAIPDTRRTD IIDRVHHACI TEGRQAYWVC TLIEESELLE
510 520 530 540 550
AQAAEATWEE LKLALPELNV GLVHGRMKPA EKQAVMASFK QGELHLLVAT
560 570 580 590 600
TVIEVGVDVP NASLMIIENP ERLGLAQLHQ LRGRVGRGAV ASHCVLLYKT
610 620 630 640 650
PLSKTAQIRL QVLRDSNDGF VIAQKDLEIR GPGELLGTRQ TGNAEFKVAD
660 670 680 690
LLRDQAMIPE VQRLARHIHE RYPQQAKALI ERWMPETERY SNA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59550 Genomic DNA Translation: CAA42123.1 M64367 Genomic DNA Translation: AAA24513.1 L10328 Genomic DNA Translation: AAA62005.1 U00096 Genomic DNA Translation: AAC76676.1 AP009048 Genomic DNA Translation: BAE77641.1 |
PIRi | JH0265 |
RefSeqi | NP_418109.1, NC_000913.3 WP_000678419.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76676; AAC76676; b3652 BAE77641; BAE77641; BAE77641 |
GeneIDi | 57729985 948162 |
KEGGi | ecj:JW3627 eco:b3652 |
PATRICi | fig|511145.12.peg.3772 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59550 Genomic DNA Translation: CAA42123.1 M64367 Genomic DNA Translation: AAA24513.1 L10328 Genomic DNA Translation: AAA62005.1 U00096 Genomic DNA Translation: AAC76676.1 AP009048 Genomic DNA Translation: BAE77641.1 |
PIRi | JH0265 |
RefSeqi | NP_418109.1, NC_000913.3 WP_000678419.1, NZ_LN832404.1 |
3D structure databases
SMRi | P24230 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262929, 148 interactors |
DIPi | DIP-10653N |
IntActi | P24230, 30 interactors |
STRINGi | 511145.b3652 |
Proteomic databases
jPOSTi | P24230 |
PaxDbi | P24230 |
PRIDEi | P24230 |
Genome annotation databases
EnsemblBacteriai | AAC76676; AAC76676; b3652 BAE77641; BAE77641; BAE77641 |
GeneIDi | 57729985 948162 |
KEGGi | ecj:JW3627 eco:b3652 |
PATRICi | fig|511145.12.peg.3772 |
Organism-specific databases
EchoBASEi | EB0822 |
Phylogenomic databases
eggNOGi | COG1200, Bacteria |
HOGENOMi | CLU_005122_7_1_6 |
InParanoidi | P24230 |
PhylomeDBi | P24230 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10829-MONOMER |
Miscellaneous databases
PROi | PR:P24230 |
Family and domain databases
InterProi | View protein in InterPro IPR004609, ATP-dep_DNA_helicase_RecG IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase IPR033454, RecG_wedge |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit PF17191, RecG_wedge, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR00643, recG, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RECG_ECOLI | |
Accessioni | P24230Primary (citable) accession number: P24230 Secondary accession number(s): P76721, Q2M7W5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | March 1, 1992 | |
Last modified: | February 10, 2021 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families