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UniProtKB - P24228 (DACB_ECOLI)
Protein
D-alanyl-D-alanine carboxypeptidase DacB
Gene
dacB
Organism
Escherichia coli (strain K12)
Status
Functioni
Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction.
1 PublicationMiscellaneous
In E.coli there are three murein endopeptidases: two are penicillin sensitive (DacB and PbpG), the other (MepA) not.
Catalytic activityi
- Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine. EC:3.4.16.4
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 62 | Acyl-ester intermediateBy similarity | 1 | |
Active sitei | 65 | Proton acceptorBy similarity | 1 | |
Active sitei | 306 | By similarity | 1 | |
Binding sitei | 417 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- carboxypeptidase activity Source: EcoCyc
- endopeptidase activity Source: EcoCyc
- penicillin binding Source: EcoCyc
- serine-type carboxypeptidase activity Source: GO_Central
- serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoliWiki
GO - Biological processi
- cell wall macromolecule catabolic process Source: EcoCyc
- cell wall organization Source: EcoCyc
- FtsZ-dependent cytokinesis Source: EcoCyc
- peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
- peptidoglycan catabolic process Source: EcoCyc
- peptidoglycan metabolic process Source: EcoCyc
- peptidoglycan turnover Source: EcoCyc
- proteolysis Source: GO_Central
- regulation of cell shape Source: EcoCyc
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Antibiotic resistance, Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10202-MONOMER |
BRENDAi | 3.4.16.4, 2165 3.4.17.14, 2026 |
UniPathwayi | UPA00219 |
Protein family/group databases
MEROPSi | S13.001 |
MoonProti | P24228 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dacB Ordered Locus Names:b3182, JW3149 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm Curated
Other locations
- periplasmic space Source: UniProtKB-SubCell
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL2354204 |
DrugBanki | DB01602, Bacampicillin DB00578, Carbenicillin DB09319, Carindacillin DB14879, Cefiderocol DB01328, Cefonicid DB01329, Cefoperazone DB01331, Cefoxitin DB09050, Ceftolozane DB01000, Cyclacillin DB00303, Ertapenem DB04570, Latamoxef DB00760, Meropenem DB00417, Phenoxymethylpenicillin |
DrugCentrali | P24228 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | 1 PublicationAdd BLAST | 20 | |
ChainiPRO_0000027241 | 21 – 477 | D-alanyl-D-alanine carboxypeptidase DacBAdd BLAST | 457 |
Proteomic databases
jPOSTi | P24228 |
PaxDbi | P24228 |
PRIDEi | P24228 |
Interactioni
Binary interactionsi
P24228
With | #Exp. | IntAct |
---|---|---|
argG [P0A6E4] | 2 | EBI-1131834,EBI-1120296 |
Protein-protein interaction databases
BioGRIDi | 4262437, 332 interactors 852006, 1 interactor |
IntActi | P24228, 1 interactor |
STRINGi | 511145.b3182 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P24228 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24228 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 90 – 263 | Absent in class-A beta-lactamasesAdd BLAST | 174 |
Sequence similaritiesi
Belongs to the peptidase S13 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG2027, Bacteria |
HOGENOMi | CLU_017692_1_1_6 |
InParanoidi | P24228 |
PhylomeDBi | P24228 |
Family and domain databases
Gene3Di | 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR000667, Peptidase_S13 |
PANTHERi | PTHR30023, PTHR30023, 1 hit |
Pfami | View protein in Pfam PF02113, Peptidase_S13, 1 hit |
PRINTSi | PR00922, DADACBPTASE3 |
SUPFAMi | SSF56601, SSF56601, 1 hit |
TIGRFAMsi | TIGR00666, PBP4, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P24228-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRFSRFIIGL TSCIAFSVQA ANVDEYITQL PAGANLALMV QKVGASAPAI
60 70 80 90 100
DYHSQQMALP ASTQKVITAL AALIQLGPDF RFTTTLETKG NVENGVLKGD
110 120 130 140 150
LVARFGADPT LKRQDIRNMV ATLKKSGVNQ IDGNVLIDTS IFASHDKAPG
160 170 180 190 200
WPWNDMTQCF SAPPAAAIVD RNCFSVSLYS APKPGDMAFI RVASYYPVTM
210 220 230 240 250
FSQVRTLPRG SAEAQYCELD VVPGDLNRFT LTGCLPQRSE PLPLAFAVQD
260 270 280 290 300
GASYAGAILK DELKQAGITW SGTLLRQTQV NEPGTVVASK QSAPLHDLLK
310 320 330 340 350
IMLKKSDNMI ADTVFRMIGH ARFNVPGTWR AGSDAVRQIL RQQAGVDIGN
360 370 380 390 400
TIIADGSGLS RHNLIAPATM MQVLQYIAQH DNELNFISML PLAGYDGSLQ
410 420 430 440 450
YRAGLHQAGV DGKVSAKTGS LQGVYNLAGF ITTASGQRMA FVQYLSGYAV
460 470
EPADQRNRRI PLVRFESRLY KDIYQNN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 261 | D → Y (PubMed:16411754).Curated | 1 | |
Sequence conflicti | 427 | L → Q in CAA42643 (PubMed:2040429).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59460 Genomic DNA Translation: CAA42070.1 X60038 Genomic DNA Translation: CAA42643.1 U01376 Genomic DNA Translation: AAA97505.1 U18997 Genomic DNA Translation: AAA57983.1 U00096 Genomic DNA Translation: AAC76214.1 AP009048 Genomic DNA Translation: BAE77226.1 |
PIRi | A54535 |
RefSeqi | NP_417649.1, NC_000913.3 WP_001212619.1, NZ_SSZK01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC76214; AAC76214; b3182 BAE77226; BAE77226; BAE77226 |
GeneIDi | 947693 |
KEGGi | ecj:JW3149 eco:b3182 |
PATRICi | fig|511145.12.peg.3275 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59460 Genomic DNA Translation: CAA42070.1 X60038 Genomic DNA Translation: CAA42643.1 U01376 Genomic DNA Translation: AAA97505.1 U18997 Genomic DNA Translation: AAA57983.1 U00096 Genomic DNA Translation: AAC76214.1 AP009048 Genomic DNA Translation: BAE77226.1 |
PIRi | A54535 |
RefSeqi | NP_417649.1, NC_000913.3 WP_001212619.1, NZ_SSZK01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2EX2 | X-ray | 1.55 | A | 21-477 | [»] | |
2EX6 | X-ray | 1.60 | A | 21-477 | [»] | |
2EX8 | X-ray | 1.60 | A | 21-477 | [»] | |
2EX9 | X-ray | 1.65 | A | 21-477 | [»] | |
2EXA | X-ray | 1.70 | A | 21-477 | [»] | |
2EXB | X-ray | 1.75 | A | 21-477 | [»] | |
SMRi | P24228 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262437, 332 interactors 852006, 1 interactor |
IntActi | P24228, 1 interactor |
STRINGi | 511145.b3182 |
Chemistry databases
ChEMBLi | CHEMBL2354204 |
DrugBanki | DB01602, Bacampicillin DB00578, Carbenicillin DB09319, Carindacillin DB14879, Cefiderocol DB01328, Cefonicid DB01329, Cefoperazone DB01331, Cefoxitin DB09050, Ceftolozane DB01000, Cyclacillin DB00303, Ertapenem DB04570, Latamoxef DB00760, Meropenem DB00417, Phenoxymethylpenicillin |
DrugCentrali | P24228 |
Protein family/group databases
MEROPSi | S13.001 |
MoonProti | P24228 |
Proteomic databases
jPOSTi | P24228 |
PaxDbi | P24228 |
PRIDEi | P24228 |
Genome annotation databases
EnsemblBacteriai | AAC76214; AAC76214; b3182 BAE77226; BAE77226; BAE77226 |
GeneIDi | 947693 |
KEGGi | ecj:JW3149 eco:b3182 |
PATRICi | fig|511145.12.peg.3275 |
Organism-specific databases
EchoBASEi | EB0198 |
Phylogenomic databases
eggNOGi | COG2027, Bacteria |
HOGENOMi | CLU_017692_1_1_6 |
InParanoidi | P24228 |
PhylomeDBi | P24228 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:EG10202-MONOMER |
BRENDAi | 3.4.16.4, 2165 3.4.17.14, 2026 |
Miscellaneous databases
EvolutionaryTracei | P24228 |
PROi | PR:P24228 |
Family and domain databases
Gene3Di | 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR000667, Peptidase_S13 |
PANTHERi | PTHR30023, PTHR30023, 1 hit |
Pfami | View protein in Pfam PF02113, Peptidase_S13, 1 hit |
PRINTSi | PR00922, DADACBPTASE3 |
SUPFAMi | SSF56601, SSF56601, 1 hit |
TIGRFAMsi | TIGR00666, PBP4, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DACB_ECOLI | |
Accessioni | P24228Primary (citable) accession number: P24228 Secondary accession number(s): Q2M930 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 169 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families