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UniProtKB - P24224 (ACPS_ECOLI)
Protein
Holo-[acyl-carrier-protein] synthase
Gene
acpS
Organism
Escherichia coli (strain K12)
Status
Functioni
Transfers the 4'-phosphopantetheine moiety from coenzyme A to the 'Ser-36' of acyl-carrier-protein.
1 PublicationCatalytic activityi
- EC:2.7.8.7UniRule annotation1 Publication
Cofactori
Mg2+UniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 9 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 58 | MagnesiumUniRule annotation | 1 |
GO - Molecular functioni
- holo-[acyl-carrier-protein] synthase activity Source: EcoliWiki
- magnesium ion binding Source: UniProtKB-UniRule
- transferase activity Source: EcoliWiki
GO - Biological processi
- fatty acid biosynthetic process Source: EcoliWiki
- peptidyl-serine phosphopantetheinylation Source: EcoliWiki
Keywordsi
Molecular function | Transferase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:HOLO-ACP-SYNTH-MONOMER |
BRENDAi | 2.7.8.7, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Holo-[acyl-carrier-protein] synthaseUniRule annotation (EC:2.7.8.7UniRule annotation)Short name: Holo-ACP synthaseUniRule annotation Alternative name(s): 4'-phosphopantetheinyl transferase AcpSUniRule annotation |
Gene namesi | Name:acpSUniRule annotation Synonyms:dpj Ordered Locus Names:b2563, JW2547 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 5 | G → D: 5-fold reduction in catalytic activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000175643 | 2 – 126 | Holo-[acyl-carrier-protein] synthaseAdd BLAST | 125 |
Proteomic databases
PaxDbi | P24224 |
PRIDEi | P24224 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260598, 286 interactors |
DIPi | DIP-9047N |
IntActi | P24224, 13 interactors |
STRINGi | 511145.b2563 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P24224 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0736, Bacteria |
HOGENOMi | CLU_089696_3_1_6 |
InParanoidi | P24224 |
PhylomeDBi | P24224 |
Family and domain databases
Gene3Di | 3.90.470.20, 1 hit |
HAMAPi | MF_00101, AcpS, 1 hit |
InterProi | View protein in InterPro IPR008278, 4-PPantetheinyl_Trfase_dom IPR037143, 4-PPantetheinyl_Trfase_dom_sf IPR002582, ACPS IPR004568, Ppantetheine-prot_Trfase_dom |
Pfami | View protein in Pfam PF01648, ACPS, 1 hit |
SUPFAMi | SSF56214, SSF56214, 1 hit |
TIGRFAMsi | TIGR00516, acpS, 1 hit TIGR00556, pantethn_trn, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P24224-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAILGLGTDI VEIARIEAVI ARSGDRLARR VLSDNEWAIW KTHHQPVRFL
60 70 80 90 100
AKRFAVKEAA AKAFGTGIRN GLAFNQFEVF NDELGKPRLR LWGEALKLAE
110 120
KLGVANMHVT LADERHYACA TVIIES
Mass spectrometryi
Molecular mass is 13950 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M76470 Genomic DNA Translation: AAA21846.1 M74526 Genomic DNA Translation: AAA24316.1 D64044 Genomic DNA No translation available. U36841 Genomic DNA Translation: AAA79825.1 U00096 Genomic DNA Translation: AAC75616.1 AP009048 Genomic DNA Translation: BAE76739.1 |
PIRi | B42294 |
RefSeqi | NP_417058.1, NC_000913.3 WP_000986023.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75616; AAC75616; b2563 BAE76739; BAE76739; BAE76739 |
GeneIDi | 947037 |
KEGGi | ecj:JW2547 eco:b2563 |
PATRICi | fig|1411691.4.peg.4171 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M76470 Genomic DNA Translation: AAA21846.1 M74526 Genomic DNA Translation: AAA24316.1 D64044 Genomic DNA No translation available. U36841 Genomic DNA Translation: AAA79825.1 U00096 Genomic DNA Translation: AAC75616.1 AP009048 Genomic DNA Translation: BAE76739.1 |
PIRi | B42294 |
RefSeqi | NP_417058.1, NC_000913.3 WP_000986023.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5VBX | X-ray | 2.05 | A/B/C | 1-126 | [»] | |
5VCB | X-ray | 4.10 | A/B/C/G/H/I/M/N/O/S/T/U/a/b/c | 1-126 | [»] | |
5XU7 | X-ray | 1.84 | A/B/C | 1-126 | [»] | |
5XUH | X-ray | 2.02 | A/B/C | 1-126 | [»] | |
SMRi | P24224 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260598, 286 interactors |
DIPi | DIP-9047N |
IntActi | P24224, 13 interactors |
STRINGi | 511145.b2563 |
Proteomic databases
PaxDbi | P24224 |
PRIDEi | P24224 |
Genome annotation databases
EnsemblBacteriai | AAC75616; AAC75616; b2563 BAE76739; BAE76739; BAE76739 |
GeneIDi | 947037 |
KEGGi | ecj:JW2547 eco:b2563 |
PATRICi | fig|1411691.4.peg.4171 |
Organism-specific databases
EchoBASEi | EB0243 |
Phylogenomic databases
eggNOGi | COG0736, Bacteria |
HOGENOMi | CLU_089696_3_1_6 |
InParanoidi | P24224 |
PhylomeDBi | P24224 |
Enzyme and pathway databases
BioCyci | EcoCyc:HOLO-ACP-SYNTH-MONOMER |
BRENDAi | 2.7.8.7, 2026 |
Miscellaneous databases
PROi | PR:P24224 |
Family and domain databases
Gene3Di | 3.90.470.20, 1 hit |
HAMAPi | MF_00101, AcpS, 1 hit |
InterProi | View protein in InterPro IPR008278, 4-PPantetheinyl_Trfase_dom IPR037143, 4-PPantetheinyl_Trfase_dom_sf IPR002582, ACPS IPR004568, Ppantetheine-prot_Trfase_dom |
Pfami | View protein in Pfam PF01648, ACPS, 1 hit |
SUPFAMi | SSF56214, SSF56214, 1 hit |
TIGRFAMsi | TIGR00516, acpS, 1 hit TIGR00556, pantethn_trn, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACPS_ECOLI | |
Accessioni | P24224Primary (citable) accession number: P24224 Secondary accession number(s): Q2MAG7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 168 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families