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Protein

Holo-[acyl-carrier-protein] synthase

Gene

acpS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transfers the 4'-phosphopantetheine moiety from coenzyme A to the 'Ser-36' of acyl-carrier-protein.1 Publication

Catalytic activityi

CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein].UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumUniRule annotation1
Metal bindingi58MagnesiumUniRule annotation1

GO - Molecular functioni

  • holo-[acyl-carrier-protein] synthase activity Source: EcoliWiki
  • magnesium ion binding Source: InterPro
  • transferase activity Source: EcoliWiki

GO - Biological processi

  • fatty acid biosynthetic process Source: EcoliWiki
  • peptidyl-serine phosphopantetheinylation Source: EcoliWiki

Keywordsi

Molecular functionTransferase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HOLO-ACP-SYNTH-MONOMER
MetaCyc:HOLO-ACP-SYNTH-MONOMER
BRENDAi2.7.8.7 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Holo-[acyl-carrier-protein] synthaseUniRule annotation (EC:2.7.8.7UniRule annotation)
Short name:
Holo-ACP synthaseUniRule annotation
Alternative name(s):
4'-phosphopantetheinyl transferase AcpSUniRule annotation
Gene namesi
Name:acpSUniRule annotation
Synonyms:dpj
Ordered Locus Names:b2563, JW2547
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10247 acpS

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi5G → D: 5-fold reduction in catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001756432 – 126Holo-[acyl-carrier-protein] synthaseAdd BLAST125

Proteomic databases

PaxDbiP24224
PRIDEiP24224

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4260598, 286 interactors
DIPiDIP-9047N
IntActiP24224, 13 interactors
STRINGi316407.85675454

Structurei

Secondary structure

1126
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 12Combined sources10
Helixi13 – 23Combined sources11
Helixi26 – 31Combined sources6
Helixi34 – 40Combined sources7
Helixi46 – 64Combined sources19
Helixi74 – 76Combined sources3
Beta strandi77 – 81Combined sources5
Beta strandi87 – 91Combined sources5
Helixi93 – 102Combined sources10
Beta strandi106 – 113Combined sources8
Beta strandi115 – 125Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5VBXX-ray2.05A/B/C1-126[»]
5VCBX-ray4.10A/B/C/G/H/I/M/N/O/S/T/U/a/b/c1-126[»]
ProteinModelPortaliP24224
SMRiP24224
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the P-Pant transferase superfamily. AcpS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KJV Bacteria
COG0736 LUCA
HOGENOMiHOG000014316
InParanoidiP24224
KOiK00997
OMAiDERHYAV
PhylomeDBiP24224

Family and domain databases

Gene3Di3.90.470.20, 1 hit
HAMAPiMF_00101 AcpS, 1 hit
InterProiView protein in InterPro
IPR008278 4-PPantetheinyl_Trfase_dom
IPR037143 4-PPantetheinyl_Trfase_dom_sf
IPR002582 ACPS
IPR004568 Ppantetheine-prot_Trfase_dom
PANTHERiPTHR45198 PTHR45198, 1 hit
PfamiView protein in Pfam
PF01648 ACPS, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004282 PPantethiene-prot_Trfase, 1 hit
SUPFAMiSSF56214 SSF56214, 1 hit
TIGRFAMsiTIGR00516 acpS, 1 hit
TIGR00556 pantethn_trn, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAILGLGTDI VEIARIEAVI ARSGDRLARR VLSDNEWAIW KTHHQPVRFL
60 70 80 90 100
AKRFAVKEAA AKAFGTGIRN GLAFNQFEVF NDELGKPRLR LWGEALKLAE
110 120
KLGVANMHVT LADERHYACA TVIIES
Length:126
Mass (Da):14,052
Last modified:January 23, 2007 - v3
Checksum:iA80628315E69731E
GO

Mass spectrometryi

Molecular mass is 13950 Da from positions 2 - 126. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76470 Genomic DNA Translation: AAA21846.1
M74526 Genomic DNA Translation: AAA24316.1
D64044 Genomic DNA No translation available.
U36841 Genomic DNA Translation: AAA79825.1
U00096 Genomic DNA Translation: AAC75616.1
AP009048 Genomic DNA Translation: BAE76739.1
PIRiB42294
RefSeqiNP_417058.1, NC_000913.3
WP_000986023.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75616; AAC75616; b2563
BAE76739; BAE76739; BAE76739
GeneIDi947037
KEGGiecj:JW2547
eco:b2563
PATRICifig|1411691.4.peg.4171

Similar proteinsi

Entry informationi

Entry nameiACPS_ECOLI
AccessioniPrimary (citable) accession number: P24224
Secondary accession number(s): Q2MAG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 149 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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