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Protein

Mannonate dehydratase

Gene

uxuA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the dehydration of D-mannonate.1 Publication

Catalytic activityi

D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O.1 Publication

Cofactori

Fe2+1 Publication, Mn2+1 PublicationNote: Mn2+ can substitute for iron, but in higher concentrations. Cannot use Fe3+, Ni2+ or Mg2+.1 Publication

Activity regulationi

Is inhibited by high concentrations of Fe2+ (> 2 mM), and by EDTA or other iron chelators in vitro.1 Publication

pH dependencei

Optimum pH is about 7.5. The activity at pH 6.5 and 8.6 is only about 20% of the maximal achievable activity at optimum pH.1 Publication

Pathwayi: pentose and glucuronate interconversion

This protein is involved in the pathway pentose and glucuronate interconversion, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway pentose and glucuronate interconversion and in Carbohydrate metabolism.

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • D-glucuronate catabolic process Source: EcoCyc

Keywordsi

Molecular functionLyase
LigandIron, Manganese

Enzyme and pathway databases

BioCyciEcoCyc:MANNONDEHYDRAT-MONOMER
MetaCyc:MANNONDEHYDRAT-MONOMER
UniPathwayi
UPA00246

Names & Taxonomyi

Protein namesi
Recommended name:
Mannonate dehydratase (EC:4.2.1.8)
Alternative name(s):
D-mannonate hydro-lyase
Gene namesi
Name:uxuA
Ordered Locus Names:b4322, JW4285
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11066 uxuA

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001706701 – 394Mannonate dehydrataseAdd BLAST394

Proteomic databases

EPDiP24215
PaxDbiP24215
PRIDEiP24215

Expressioni

Inductioni

By fructuronate. Its expression is subjected to catabolite repression by glucose.

Interactioni

Protein-protein interaction databases

BioGridi4261003, 15 interactors
DIPiDIP-11108N
IntActiP24215, 2 interactors
STRINGi316407.85677065

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP24215
SMRiP24215
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mannonate dehydratase family.Curated

Phylogenomic databases

eggNOGiENOG4105CHZ Bacteria
COG1312 LUCA
HOGENOMiHOG000195381
InParanoidiP24215
KOiK01686
PhylomeDBiP24215

Family and domain databases

HAMAPiMF_00106 UxuA, 1 hit
InterProiView protein in InterPro
IPR004628 Man_deHydtase
IPR036237 Xyl_isomerase-like_sf
PANTHERiPTHR30387 PTHR30387, 1 hit
PfamiView protein in Pfam
PF03786 UxuA, 1 hit
PIRSFiPIRSF016049 Man_dehyd, 1 hit
SUPFAMiSSF51658 SSF51658, 1 hit
TIGRFAMsiTIGR00695 uxuA, 1 hit

Sequencei

Sequence statusi: Complete.

P24215-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEQTWRWYGP NDPVSLADVR QAGATGVVTA LHHIPNGEVW SVEEILKRKA
60 70 80 90 100
IIEDAGLVWS VVESVPIHED IKTHTGNYEQ WIANYQQTLR NLAQCGIRTV
110 120 130 140 150
CYNFMPVLDW TRTDLEYVLP DGSKALRFDQ IEFAAFEMHI LKRPGAEADY
160 170 180 190 200
TEEEIAQAAE RFATMSDEDK ARLTRNIIAG LPGAEEGYTL DQFRKHLELY
210 220 230 240 250
KDIDKAKLRE NFAVFLKAII PVAEEVGVRM AVHPDDPPRP ILGLPRIVST
260 270 280 290 300
IEDMQWMVDT VNSMANGFTM CTGSYGVRAD NDLVDMIKQF GPRIYFTHLR
310 320 330 340 350
STMREDNPKT FHEAAHLNGD VDMYEVVKAI VEEEHRRKAE GKEDLIPMRP
360 370 380 390
DHGHQMLDDL KKKTNPGYSA IGRLKGLAEV RGVELAIQRA FFSR
Length:394
Mass (Da):44,838
Last modified:February 1, 1995 - v2
Checksum:i0716A50BD6436679
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti52I → V in strain: MT78. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13329 Genomic DNA Translation: BAA02590.1
U14003 Genomic DNA Translation: AAA97218.1
U00096 Genomic DNA Translation: AAC77278.1
AP009048 Genomic DNA Translation: BAE78315.1
AJ225176 Genomic DNA Translation: CAA12424.1
X03411 Genomic DNA Translation: CAA27147.1 Different termination.
X91735 Genomic DNA Translation: CAA62860.1
PIRiI57745
S56547
RefSeqiNP_418742.1, NC_000913.3
WP_000438562.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77278; AAC77278; b4322
BAE78315; BAE78315; BAE78315
GeneIDi947082
KEGGiecj:JW4285
eco:b4322
PATRICifig|1411691.4.peg.2370

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13329 Genomic DNA Translation: BAA02590.1
U14003 Genomic DNA Translation: AAA97218.1
U00096 Genomic DNA Translation: AAC77278.1
AP009048 Genomic DNA Translation: BAE78315.1
AJ225176 Genomic DNA Translation: CAA12424.1
X03411 Genomic DNA Translation: CAA27147.1 Different termination.
X91735 Genomic DNA Translation: CAA62860.1
PIRiI57745
S56547
RefSeqiNP_418742.1, NC_000913.3
WP_000438562.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EACX-ray2.30A/B/C/D1-394[»]
4EAYX-ray2.35A/B/C/D1-394[»]
ProteinModelPortaliP24215
SMRiP24215
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261003, 15 interactors
DIPiDIP-11108N
IntActiP24215, 2 interactors
STRINGi316407.85677065

Proteomic databases

EPDiP24215
PaxDbiP24215
PRIDEiP24215

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77278; AAC77278; b4322
BAE78315; BAE78315; BAE78315
GeneIDi947082
KEGGiecj:JW4285
eco:b4322
PATRICifig|1411691.4.peg.2370

Organism-specific databases

EchoBASEiEB1059
EcoGeneiEG11066 uxuA

Phylogenomic databases

eggNOGiENOG4105CHZ Bacteria
COG1312 LUCA
HOGENOMiHOG000195381
InParanoidiP24215
KOiK01686
PhylomeDBiP24215

Enzyme and pathway databases

UniPathwayi
UPA00246

BioCyciEcoCyc:MANNONDEHYDRAT-MONOMER
MetaCyc:MANNONDEHYDRAT-MONOMER

Miscellaneous databases

PROiPR:P24215

Family and domain databases

HAMAPiMF_00106 UxuA, 1 hit
InterProiView protein in InterPro
IPR004628 Man_deHydtase
IPR036237 Xyl_isomerase-like_sf
PANTHERiPTHR30387 PTHR30387, 1 hit
PfamiView protein in Pfam
PF03786 UxuA, 1 hit
PIRSFiPIRSF016049 Man_dehyd, 1 hit
SUPFAMiSSF51658 SSF51658, 1 hit
TIGRFAMsiTIGR00695 uxuA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUXUA_ECOLI
AccessioniPrimary (citable) accession number: P24215
Secondary accession number(s): O87739, Q2M5Z1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 1, 1995
Last modified: November 7, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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