UniProtKB - P24203 (YJIA_ECOLI)
Protein
P-loop guanosine triphosphatase YjiA
Gene
yjiA
Organism
Escherichia coli (strain K12)
Status
Functioni
Binds GTP and has low GTPase activity. May have a GTP-dependent regulatory function.2 Publications
Activity regulationi
GTPase activity is inhibited by metal binding. Activity is decreased in the presence of Co(II) or Ni(II), and is completely inhibited in the presence of Zn(II).1 Publication
Kineticsi
kcat is 0.006 sec(-1) for GTP hydrolysis in the absence of metal. kcat is 0.0005 sec(-1) for GTP hydrolysis in the presence of Co(II).1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 37 | Zinc 11 Publication | 1 | |
Metal bindingi | 42 | Zinc 11 Publication | 1 | |
Metal bindingi | 66 | Zinc 11 Publication | 1 | |
Metal bindingi | 74 | Zinc 2; shared with dimeric partner1 Publication | 1 | |
Metal bindingi | 114 | Zinc 2; shared with dimeric partner1 Publication | 1 | |
Binding sitei | 161 | GTPBy similarity | 1 | |
Metal bindingi | 167 | Zinc 31 Publication | 1 | |
Metal bindingi | 167 | Zinc 4; shared with dimeric partner1 Publication | 1 | |
Metal bindingi | 170 | Zinc 3; via pros nitrogen1 Publication | 1 | |
Metal bindingi | 170 | Zinc 4; via pros nitrogen; shared with dimeric partner1 Publication | 1 | |
Metal bindingi | 187 | Zinc 3; shared with dimeric partner1 Publication | 1 | |
Metal bindingi | 187 | Zinc 41 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 11 – 19 | GTPBy similarity | 9 |
GO - Molecular functioni
- GTPase activity Source: EcoCyc
- GTP binding Source: UniProtKB-KW
- identical protein binding Source: EcoCyc
- transition metal ion binding Source: EcoCyc
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10021-MONOMER MetaCyc:EG10021-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: P-loop guanosine triphosphatase YjiA1 Publication (EC:3.6.-.-2 Publications)Alternative name(s): GTP-binding protein YjiACurated |
Gene namesi | Name:yjiA Ordered Locus Names:b4352, JW5790 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169785 | 1 – 318 | P-loop guanosine triphosphatase YjiASequence analysisAdd BLAST | 318 |
Proteomic databases
jPOSTi | P24203 |
PaxDbi | P24203 |
PRIDEi | P24203 |
Interactioni
Subunit structurei
Monomer in the apo form. Metal binding induces oligomerization. Forms homodimers and higher oligomers.
1 PublicationGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4262771, 37 interactors 853160, 1 interactor |
DIPi | DIP-12630N |
IntActi | P24203, 2 interactors |
STRINGi | 511145.b4352 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P24203 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24203 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 224 – 315 | CobW C-terminalSequence analysisAdd BLAST | 92 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0523, Bacteria |
HOGENOMi | CLU_017452_0_2_6 |
InParanoidi | P24203 |
PhylomeDBi | P24203 |
Family and domain databases
Gene3Di | 3.30.1220.10, 1 hit |
InterProi | View protein in InterPro IPR011629, Cbl_biosynth_CobW-like_C IPR036627, CobW-likC_sf IPR003495, CobW/HypB/UreG_dom IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF02492, cobW, 1 hit PF07683, CobW_C, 1 hit |
SMARTi | View protein in SMART SM00833, CobW_C, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
i Sequence
Sequence statusi: Complete.
P24203-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNPIAVTLLT GFLGAGKTTL LRHILNEQHG YKIAVIENEF GEVSVDDQLI
60 70 80 90 100
GDRATQIKTL TNGCICCSRS NELEDALLDL LDNLDKGNIQ FDRLVIECTG
110 120 130 140 150
MADPGPIIQT FFSHEVLCQR YLLDGVIALV DAVHADEQMN QFTIAQSQVG
160 170 180 190 200
YADRILLTKT DVAGEAEKLH ERLARINARA PVYTVTHGDI DLGLLFNTNG
210 220 230 240 250
FMLEENVVST KPRFHFIADK QNDISSIVVE LDYPVDISEV SRVMENLLLE
260 270 280 290 300
SADKLLRYKG MLWIDGEPNR LLFQGVQRLY SADWDRPWGD EKPHSTMVFI
310
GIQLPEEEIR AAFAGLRK
Sequence cautioni
The sequence AAA97249 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97249.1 Frameshift. U00096 Genomic DNA Translation: AAC77308.2 AP009048 Genomic DNA Translation: BAE78342.1 X54198 Genomic DNA Translation: CAA38118.1 |
PIRi | S56578 |
RefSeqi | NP_418772.4, NC_000913.3 WP_001312515.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77308; AAC77308; b4352 BAE78342; BAE78342; BAE78342 |
GeneIDi | 948882 |
KEGGi | ecj:JW5790 eco:b4352 |
PATRICi | fig|1411691.4.peg.2334 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97249.1 Frameshift. U00096 Genomic DNA Translation: AAC77308.2 AP009048 Genomic DNA Translation: BAE78342.1 X54198 Genomic DNA Translation: CAA38118.1 |
PIRi | S56578 |
RefSeqi | NP_418772.4, NC_000913.3 WP_001312515.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NIJ | X-ray | 2.00 | A | 1-318 | [»] | |
4IXM | X-ray | 2.57 | A/B | 1-318 | [»] | |
4IXN | X-ray | 2.05 | A/B | 1-318 | [»] | |
SMRi | P24203 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262771, 37 interactors 853160, 1 interactor |
DIPi | DIP-12630N |
IntActi | P24203, 2 interactors |
STRINGi | 511145.b4352 |
Proteomic databases
jPOSTi | P24203 |
PaxDbi | P24203 |
PRIDEi | P24203 |
Genome annotation databases
EnsemblBacteriai | AAC77308; AAC77308; b4352 BAE78342; BAE78342; BAE78342 |
GeneIDi | 948882 |
KEGGi | ecj:JW5790 eco:b4352 |
PATRICi | fig|1411691.4.peg.2334 |
Organism-specific databases
EchoBASEi | EB0020 |
Phylogenomic databases
eggNOGi | COG0523, Bacteria |
HOGENOMi | CLU_017452_0_2_6 |
InParanoidi | P24203 |
PhylomeDBi | P24203 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10021-MONOMER MetaCyc:EG10021-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P24203 |
PROi | PR:P24203 |
Family and domain databases
Gene3Di | 3.30.1220.10, 1 hit |
InterProi | View protein in InterPro IPR011629, Cbl_biosynth_CobW-like_C IPR036627, CobW-likC_sf IPR003495, CobW/HypB/UreG_dom IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF02492, cobW, 1 hit PF07683, CobW_C, 1 hit |
SMARTi | View protein in SMART SM00833, CobW_C, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YJIA_ECOLI | |
Accessioni | P24203Primary (citable) accession number: P24203 Secondary accession number(s): Q2M5W4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | April 27, 2001 | |
Last modified: | April 7, 2021 | |
This is version 149 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families