UniProtKB - P24197 (YGID_ECOLI)
Protein
4,5-DOPA dioxygenase extradiol
Gene
ygiD
Organism
Escherichia coli (strain K12)
Status
Functioni
In vitro, opens the cyclic ring of dihydroxy-phenylalanine (DOPA) between carbons 4 and 5, thus producing an unstable seco-DOPA that rearranges nonenzymatically to betalamic acid. The physiological substrate is unknown.1 Publication
Miscellaneous
Betalamic acid is the structural unit of the betalains, natural nitrogen-containing water-soluble pigments with high colorant and bioactive properties, characteristic of plants of the order Caryophyllales.1 Publication
Caution
It is uncertain whether Met-1 or Met-10 is the initiator.Curated
Catalytic activityi
- EC:1.13.11.291 Publication
Cofactori
Zn2+By similarity
Kineticsi
kcat is 0.17 min(-1) with L-DOPA.1 Publication
- KM=7.9 mM for L-DOPA1 Publication
pH dependencei
Optimum pH is 8.0.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 22 | Zinc1 Publication | 1 | |
Metal bindingi | 57 | Zinc1 Publication | 1 | |
Metal bindingi | 177 | Zinc1 Publication | 1 | |
Metal bindingi | 234 | Zinc1 Publication | 1 |
GO - Molecular functioni
- DOPA dioxygenase activity Source: EcoCyc
- ferrous iron binding Source: InterPro
- stizolobate synthase activity Source: UniProtKB-EC
- zinc ion binding Source: InterPro
GO - Biological processi
- cellular aromatic compound metabolic process Source: InterPro
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11166-MONOMER MetaCyc:EG11166-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ygiD Ordered Locus Names:b3039, JW3007 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm 1 Publication
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169405 | 1 – 271 | 4,5-DOPA dioxygenase extradiolAdd BLAST | 271 |
Proteomic databases
PaxDbi | P24197 |
PRIDEi | P24197 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4263035, 3 interactors |
IntActi | P24197, 3 interactors |
STRINGi | 511145.b3039 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P24197 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24197 |
Family & Domainsi
Sequence similaritiesi
Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.Curated
Phylogenomic databases
eggNOGi | COG3384, Bacteria |
HOGENOMi | CLU_046582_2_0_6 |
InParanoidi | P24197 |
Family and domain databases
CDDi | cd07363, 45_DOPA_Dioxygenase, 1 hit |
InterProi | View protein in InterPro IPR014436, Extradiol_dOase_DODA IPR004183, Xdiol_dOase_suB |
Pfami | View protein in Pfam PF02900, LigB, 1 hit |
PIRSFi | PIRSF006157, Doxgns_DODA, 1 hit |
i Sequence
Sequence statusi: Complete.
P24197-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTPLVKDIIM SSTRMPALFL GHGSPMNVLE DNLYTRSWQK LGMTLPRPQA
60 70 80 90 100
IVVVSAHWFT RGTGVTAMET PPTIHDFGGF PQALYDTHYP APGSPALAQR
110 120 130 140 150
LVELLAPIPV TLDKEAWGFD HGSWGVLIKM YPDADIPMVQ LSIDSSKPAA
160 170 180 190 200
WHFEMGRKLA ALRDEGIMLV ASGNVVHNLR TVKWHGDSSP YPWATSFNEY
210 220 230 240 250
VKANLTWQGP VEQHPLVNYL DHEGGTLSNP TPEHYLPLLY VLGAWDGQEP
260 270
ITIPVEGIEM GSLSMLSVQI G
Sequence cautioni
The sequence AAC76075 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 243 – 270 | GAWDG…LSVQI → RYVGWAGANYHS in AAA71877 (PubMed:1314093).CuratedAdd BLAST | 28 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M77129 Genomic DNA Translation: AAA71877.1 U28377 Genomic DNA Translation: AAA69207.1 U00096 Genomic DNA Translation: AAC76075.2 Different initiation. AP009048 Genomic DNA Translation: BAE77095.1 |
PIRi | E65091 |
RefSeqi | NP_417511.2, NC_000913.3 WP_000188362.1, NZ_CP047127.1 |
Genome annotation databases
EnsemblBacteriai | AAC76075; AAC76075; b3039 BAE77095; BAE77095; BAE77095 |
GeneIDi | 946447 |
KEGGi | ecj:JW3007 eco:b3039 |
PATRICi | fig|1411691.4.peg.3693 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M77129 Genomic DNA Translation: AAA71877.1 U28377 Genomic DNA Translation: AAA69207.1 U00096 Genomic DNA Translation: AAC76075.2 Different initiation. AP009048 Genomic DNA Translation: BAE77095.1 |
PIRi | E65091 |
RefSeqi | NP_417511.2, NC_000913.3 WP_000188362.1, NZ_CP047127.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2PW6 | X-ray | 2.27 | A | 1-271 | [»] | |
SMRi | P24197 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263035, 3 interactors |
IntActi | P24197, 3 interactors |
STRINGi | 511145.b3039 |
Proteomic databases
PaxDbi | P24197 |
PRIDEi | P24197 |
Genome annotation databases
EnsemblBacteriai | AAC76075; AAC76075; b3039 BAE77095; BAE77095; BAE77095 |
GeneIDi | 946447 |
KEGGi | ecj:JW3007 eco:b3039 |
PATRICi | fig|1411691.4.peg.3693 |
Organism-specific databases
EchoBASEi | EB1154 |
Phylogenomic databases
eggNOGi | COG3384, Bacteria |
HOGENOMi | CLU_046582_2_0_6 |
InParanoidi | P24197 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11166-MONOMER MetaCyc:EG11166-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P24197 |
PROi | PR:P24197 |
Family and domain databases
CDDi | cd07363, 45_DOPA_Dioxygenase, 1 hit |
InterProi | View protein in InterPro IPR014436, Extradiol_dOase_DODA IPR004183, Xdiol_dOase_suB |
Pfami | View protein in Pfam PF02900, LigB, 1 hit |
PIRSFi | PIRSF006157, Doxgns_DODA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YGID_ECOLI | |
Accessioni | P24197Primary (citable) accession number: P24197 Secondary accession number(s): Q2M9G1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | November 2, 2016 | |
Last modified: | December 2, 2020 | |
This is version 144 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families