UniProtKB - P24193 (HYPE_ECOLI)
Protein
Carbamoyl dehydratase HypE
Gene
hypE
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the maturation of [NiFe] hydrogenases. Along with HypF, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases. HypE catalyzes the ATP-dependent dehydration of the carboxamido group attached to its C-terminal cysteine to a cyano group (PubMed:12586941, PubMed:15291820). The cyano group is then transferred from HypE to the HypC-HypD complex or the HybG-HypD complex (PubMed:15504408).3 Publications
Catalytic activityi
- ATP + C-terminal S-carboxamide-L-cysteinyl-[HypE protein] = ADP + C-terminal S-cyanate-L-cysteinyl-[HypE protein] + H+ + phosphate2 Publications
: [NiFe] hydrogenase maturation Pathwayi
This protein is involved in the pathway [NiFe] hydrogenase maturation, which is part of Protein modification.CuratedView all proteins of this organism that are known to be involved in the pathway [NiFe] hydrogenase maturation and in Protein modification.
GO - Molecular functioni
- lyase activity Source: UniProtKB-KW
GO - Biological processi
- peptidyl-S-carbamoyl-L-cysteine dehydration Source: EcoCyc
- protein maturation Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10487-MONOMER MetaCyc:EG10487-MONOMER |
UniPathwayi | UPA00335 |
Names & Taxonomyi
Protein namesi | Recommended name: Carbamoyl dehydratase HypECurated (EC:4.2.1.-2 Publications)Alternative name(s): Hydrogenase maturation factor HypECurated |
Gene namesi | Name:hypE1 Publication Ordered Locus Names:b2730, JW2700 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 83 | D → N: Lack of ATPase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201459 | 1 – 336 | Carbamoyl dehydratase HypEAdd BLAST | 336 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 336 | S-carbamoylcysteine; by HypF; alternate2 Publications | 1 | |
Modified residuei | 336 | S-cyanocysteine; by autocatalysis; alternate2 Publications | 1 |
Post-translational modificationi
Modified by HypF, which adds a carboxamido group to the thiolate of the C-terminal cysteine, yielding a protein-S-carboxamide. The carboxamido group is then dehydrated by HypE itself to yield a protein-thiocyanate.2 Publications
Proteomic databases
jPOSTi | P24193 |
PaxDbi | P24193 |
PRIDEi | P24193 |
Interactioni
Subunit structurei
Binary interactionsi
P24193
With | #Exp. | IntAct |
---|---|---|
hypF [P30131] | 2 | EBI-552743,EBI-9128507 |
Protein-protein interaction databases
BioGRIDi | 4261424, 23 interactors 851514, 6 interactors |
ComplexPortali | CPX-5281, HypEF Ni-hydrogenase maturation complex CPX-5283, Hybg-HypDE Ni-hydrogenase maturation complex CPX-5284, HypCDE Ni-hydrogenase maturation complex |
DIPi | DIP-9999N |
IntActi | P24193, 10 interactors |
STRINGi | 511145.b2730 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P24193 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24193 |
Family & Domainsi
Sequence similaritiesi
Belongs to the HypE family.Curated
Phylogenomic databases
eggNOGi | COG0309, Bacteria |
HOGENOMi | CLU_049733_0_0_6 |
InParanoidi | P24193 |
PhylomeDBi | P24193 |
Family and domain databases
CDDi | cd02197, HypE, 1 hit |
Gene3Di | 3.30.1330.10, 1 hit 3.90.650.10, 1 hit |
InterProi | View protein in InterPro IPR011854, HypE IPR010918, PurM-like_C_dom IPR036676, PurM-like_C_sf IPR016188, PurM-like_N IPR036921, PurM-like_N_sf |
PANTHERi | PTHR30303, PTHR30303, 1 hit |
Pfami | View protein in Pfam PF00586, AIRS, 1 hit PF02769, AIRS_C, 1 hit |
PIRSFi | PIRSF005644, Hdrgns_mtr_HypE, 1 hit |
SUPFAMi | SSF55326, SSF55326, 1 hit SSF56042, SSF56042, 1 hit |
TIGRFAMsi | TIGR02124, hypE, 1 hit |
i Sequence
Sequence statusi: Complete.
P24193-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNNIQLAHGS GGQAMQQLIN SLFMEAFANP WLAEQEDQAR LDLAQLVAEG
60 70 80 90 100
DRLAFSTDSY VIDPLFFPGG NIGKLAICGT ANDVAVSGAI PRYLSCGFIL
110 120 130 140 150
EEGLPMETLK AVVTSMAETA RAAGIAIVTG DTKVVQRGAV DKLFINTAGM
160 170 180 190 200
GAIPANIHWG AQTLTAGDVL LVSGTLGDHG ATILNLREQL GLDGELVSDC
210 220 230 240 250
AVLTPLIQTL RDIPGVKALR DATRGGVNAV VHEFAAACGC GIELSEAALP
260 270 280 290 300
VKPAVRGVCE LLGLDALNFA NEGKLVIAVE RNAAEQVLAA LHSHPLGKDA
310 320 330
ALIGEVVERK GVRLAGLYGV KRTLDLPHAE PLPRIC
Sequence cautioni
The sequence AAA69240 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE76807 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA38416 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54543 Genomic DNA Translation: CAA38416.1 Different initiation. U29579 Genomic DNA Translation: AAA69240.1 Different initiation. U00096 Genomic DNA Translation: AAC75772.2 AP009048 Genomic DNA Translation: BAE76807.1 Different initiation. |
PIRi | S15201 |
RefSeqi | NP_417210.2, NC_000913.3 WP_001059922.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75772; AAC75772; b2730 BAE76807; BAE76807; BAE76807 |
GeneIDi | 947182 |
KEGGi | ecj:JW2700 eco:b2730 |
PATRICi | fig|1411691.4.peg.4011 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54543 Genomic DNA Translation: CAA38416.1 Different initiation. U29579 Genomic DNA Translation: AAA69240.1 Different initiation. U00096 Genomic DNA Translation: AAC75772.2 AP009048 Genomic DNA Translation: BAE76807.1 Different initiation. |
PIRi | S15201 |
RefSeqi | NP_417210.2, NC_000913.3 WP_001059922.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2I6R | X-ray | 2.51 | A/B/C/D | 15-336 | [»] | |
SMRi | P24193 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261424, 23 interactors 851514, 6 interactors |
ComplexPortali | CPX-5281, HypEF Ni-hydrogenase maturation complex CPX-5283, Hybg-HypDE Ni-hydrogenase maturation complex CPX-5284, HypCDE Ni-hydrogenase maturation complex |
DIPi | DIP-9999N |
IntActi | P24193, 10 interactors |
STRINGi | 511145.b2730 |
Proteomic databases
jPOSTi | P24193 |
PaxDbi | P24193 |
PRIDEi | P24193 |
Genome annotation databases
EnsemblBacteriai | AAC75772; AAC75772; b2730 BAE76807; BAE76807; BAE76807 |
GeneIDi | 947182 |
KEGGi | ecj:JW2700 eco:b2730 |
PATRICi | fig|1411691.4.peg.4011 |
Organism-specific databases
EchoBASEi | EB0482 |
Phylogenomic databases
eggNOGi | COG0309, Bacteria |
HOGENOMi | CLU_049733_0_0_6 |
InParanoidi | P24193 |
PhylomeDBi | P24193 |
Enzyme and pathway databases
UniPathwayi | UPA00335 |
BioCyci | EcoCyc:EG10487-MONOMER MetaCyc:EG10487-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P24193 |
PROi | PR:P24193 |
Family and domain databases
CDDi | cd02197, HypE, 1 hit |
Gene3Di | 3.30.1330.10, 1 hit 3.90.650.10, 1 hit |
InterProi | View protein in InterPro IPR011854, HypE IPR010918, PurM-like_C_dom IPR036676, PurM-like_C_sf IPR016188, PurM-like_N IPR036921, PurM-like_N_sf |
PANTHERi | PTHR30303, PTHR30303, 1 hit |
Pfami | View protein in Pfam PF00586, AIRS, 1 hit PF02769, AIRS_C, 1 hit |
PIRSFi | PIRSF005644, Hdrgns_mtr_HypE, 1 hit |
SUPFAMi | SSF55326, SSF55326, 1 hit SSF56042, SSF56042, 1 hit |
TIGRFAMsi | TIGR02124, hypE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HYPE_ECOLI | |
Accessioni | P24193Primary (citable) accession number: P24193 Secondary accession number(s): Q2MA99, Q46886 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | May 5, 2009 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families