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Entry version 142 (07 Oct 2020)
Sequence version 2 (05 May 2009)
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Protein

Carbamoyl dehydratase HypE

Gene

hypE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the maturation of [NiFe] hydrogenases. Along with HypF, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases. HypE catalyzes the ATP-dependent dehydration of the carboxamido group attached to its C-terminal cysteine to a cyano group (PubMed:12586941, PubMed:15291820). The cyano group is then transferred from HypE to the HypC-HypD complex or the HybG-HypD complex (PubMed:15504408).3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: [NiFe] hydrogenase maturation

This protein is involved in the pathway [NiFe] hydrogenase maturation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway [NiFe] hydrogenase maturation and in Protein modification.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

  • peptidyl-S-carbamoyl-L-cysteine dehydration Source: EcoCyc
  • protein maturation Source: EcoCyc

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLyase

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:EG10487-MONOMER
MetaCyc:EG10487-MONOMER

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00335

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Carbamoyl dehydratase HypECurated (EC:4.2.1.-2 Publications)
Alternative name(s):
Hydrogenase maturation factor HypECurated
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:hypE1 Publication
Ordered Locus Names:b2730, JW2700
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi83D → N: Lack of ATPase activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002014591 – 336Carbamoyl dehydratase HypEAdd BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei336S-carbamoylcysteine; by HypF; alternate2 Publications1
Modified residuei336S-cyanocysteine; by autocatalysis; alternate2 Publications1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Modified by HypF, which adds a carboxamido group to the thiolate of the C-terminal cysteine, yielding a protein-S-carboxamide. The carboxamido group is then dehydrated by HypE itself to yield a protein-thiocyanate.2 Publications

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P24193

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P24193

PRoteomics IDEntifications database

More...
PRIDEi
P24193

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer (PubMed:18065529).

Forms a complex with HypF (PubMed:15291820, PubMed:18065529). Also forms a complex with HypC, or HybG, and HypD (PubMed:15504408, PubMed:16412426).

4 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4261424, 23 interactors
851514, 6 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-5281, HypEF Ni-hydrogenase maturation complex
CPX-5283, Hybg-HypDE Ni-hydrogenase maturation complex
CPX-5284, HypCDE Ni-hydrogenase maturation complex

Database of interacting proteins

More...
DIPi
DIP-9999N

Protein interaction database and analysis system

More...
IntActi
P24193, 10 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b2730

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P24193

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P24193

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the HypE family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0309, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_049733_0_0_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P24193

KEGG Orthology (KO)

More...
KOi
K04655

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P24193

Family and domain databases

Conserved Domains Database

More...
CDDi
cd02197, HypE, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.1330.10, 1 hit
3.90.650.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011854, HypE
IPR010918, PurM-like_C_dom
IPR036676, PurM-like_C_sf
IPR016188, PurM-like_N
IPR036921, PurM-like_N_sf

The PANTHER Classification System

More...
PANTHERi
PTHR30303, PTHR30303, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00586, AIRS, 1 hit
PF02769, AIRS_C, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF005644, Hdrgns_mtr_HypE, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55326, SSF55326, 1 hit
SSF56042, SSF56042, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02124, hypE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P24193-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNNIQLAHGS GGQAMQQLIN SLFMEAFANP WLAEQEDQAR LDLAQLVAEG
60 70 80 90 100
DRLAFSTDSY VIDPLFFPGG NIGKLAICGT ANDVAVSGAI PRYLSCGFIL
110 120 130 140 150
EEGLPMETLK AVVTSMAETA RAAGIAIVTG DTKVVQRGAV DKLFINTAGM
160 170 180 190 200
GAIPANIHWG AQTLTAGDVL LVSGTLGDHG ATILNLREQL GLDGELVSDC
210 220 230 240 250
AVLTPLIQTL RDIPGVKALR DATRGGVNAV VHEFAAACGC GIELSEAALP
260 270 280 290 300
VKPAVRGVCE LLGLDALNFA NEGKLVIAVE RNAAEQVLAA LHSHPLGKDA
310 320 330
ALIGEVVERK GVRLAGLYGV KRTLDLPHAE PLPRIC
Length:336
Mass (Da):35,092
Last modified:May 5, 2009 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i0811EE415F11AF6C
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA69240 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE76807 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA38416 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X54543 Genomic DNA Translation: CAA38416.1 Different initiation.
U29579 Genomic DNA Translation: AAA69240.1 Different initiation.
U00096 Genomic DNA Translation: AAC75772.2
AP009048 Genomic DNA Translation: BAE76807.1 Different initiation.

Protein sequence database of the Protein Information Resource

More...
PIRi
S15201

NCBI Reference Sequences

More...
RefSeqi
NP_417210.2, NC_000913.3
WP_001059922.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC75772; AAC75772; b2730
BAE76807; BAE76807; BAE76807

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
947182

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW2700
eco:b2730

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.4011

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54543 Genomic DNA Translation: CAA38416.1 Different initiation.
U29579 Genomic DNA Translation: AAA69240.1 Different initiation.
U00096 Genomic DNA Translation: AAC75772.2
AP009048 Genomic DNA Translation: BAE76807.1 Different initiation.
PIRiS15201
RefSeqiNP_417210.2, NC_000913.3
WP_001059922.1, NZ_LN832404.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I6RX-ray2.51A/B/C/D15-336[»]
SMRiP24193
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4261424, 23 interactors
851514, 6 interactors
ComplexPortaliCPX-5281, HypEF Ni-hydrogenase maturation complex
CPX-5283, Hybg-HypDE Ni-hydrogenase maturation complex
CPX-5284, HypCDE Ni-hydrogenase maturation complex
DIPiDIP-9999N
IntActiP24193, 10 interactors
STRINGi511145.b2730

Proteomic databases

jPOSTiP24193
PaxDbiP24193
PRIDEiP24193

Genome annotation databases

EnsemblBacteriaiAAC75772; AAC75772; b2730
BAE76807; BAE76807; BAE76807
GeneIDi947182
KEGGiecj:JW2700
eco:b2730
PATRICifig|1411691.4.peg.4011

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0482

Phylogenomic databases

eggNOGiCOG0309, Bacteria
HOGENOMiCLU_049733_0_0_6
InParanoidiP24193
KOiK04655
PhylomeDBiP24193

Enzyme and pathway databases

UniPathwayiUPA00335
BioCyciEcoCyc:EG10487-MONOMER
MetaCyc:EG10487-MONOMER

Miscellaneous databases

EvolutionaryTraceiP24193

Protein Ontology

More...
PROi
PR:P24193

Family and domain databases

CDDicd02197, HypE, 1 hit
Gene3Di3.30.1330.10, 1 hit
3.90.650.10, 1 hit
InterProiView protein in InterPro
IPR011854, HypE
IPR010918, PurM-like_C_dom
IPR036676, PurM-like_C_sf
IPR016188, PurM-like_N
IPR036921, PurM-like_N_sf
PANTHERiPTHR30303, PTHR30303, 1 hit
PfamiView protein in Pfam
PF00586, AIRS, 1 hit
PF02769, AIRS_C, 1 hit
PIRSFiPIRSF005644, Hdrgns_mtr_HypE, 1 hit
SUPFAMiSSF55326, SSF55326, 1 hit
SSF56042, SSF56042, 1 hit
TIGRFAMsiTIGR02124, hypE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHYPE_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P24193
Secondary accession number(s): Q2MA99, Q46886
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: May 5, 2009
Last modified: October 7, 2020
This is version 142 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
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