We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P24186 (FOLD_ECOLI)
Protein
Bifunctional protein FolD
Gene
folD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. This enzyme is specific for NADP.
UniRule annotation1 PublicationCatalytic activityi
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPHUniRule annotationEC:1.5.1.5UniRule annotation
- EC:3.5.4.9UniRule annotation
: tetrahydrofolate interconversion Pathwayi
This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 232 | NADP; via amide nitrogenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 166 – 168 | NADPUniRule annotation | 3 |
GO - Molecular functioni
- methenyltetrahydrofolate cyclohydrolase activity Source: EcoCyc
- methylenetetrahydrofolate dehydrogenase (NADP+) activity Source: GO_Central
GO - Biological processi
- histidine biosynthetic process Source: UniProtKB-KW
- methionine biosynthetic process Source: UniProtKB-KW
- purine nucleotide biosynthetic process Source: UniProtKB-KW
- tetrahydrofolate interconversion Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Multifunctional enzyme, Oxidoreductase |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:FOLD-MONOMER |
BRENDAi | 1.5.1.5, 2026 3.5.4.9, 2026 |
SABIO-RKi | P24186 |
UniPathwayi | UPA00193 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:folDUniRule annotation Synonyms:ads Ordered Locus Names:b0529, JW0518 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000199306 | 2 – 288 | Bifunctional protein FolDAdd BLAST | 287 |
Proteomic databases
jPOSTi | P24186 |
PaxDbi | P24186 |
PRIDEi | P24186 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation2 PublicationsProtein-protein interaction databases
BioGRIDi | 4262819, 297 interactors |
DIPi | DIP-9675N |
IntActi | P24186, 4 interactors |
STRINGi | 511145.b0529 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P24186 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24186 |
Family & Domainsi
Sequence similaritiesi
Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0190, Bacteria |
HOGENOMi | CLU_034045_2_1_6 |
InParanoidi | P24186 |
PhylomeDBi | P24186 |
Family and domain databases
CDDi | cd01080, NAD_bind_m-THF_DH_Cyclohyd, 1 hit |
HAMAPi | MF_01576, THF_DHG_CYH, 1 hit |
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR000672, THF_DH/CycHdrlase IPR020630, THF_DH/CycHdrlase_cat_dom IPR020867, THF_DH/CycHdrlase_CS IPR020631, THF_DH/CycHdrlase_NAD-bd_dom |
Pfami | View protein in Pfam PF00763, THF_DHG_CYH, 1 hit PF02882, THF_DHG_CYH_C, 1 hit |
PRINTSi | PR00085, THFDHDRGNASE |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00766, THF_DHG_CYH_1, 1 hit PS00767, THF_DHG_CYH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P24186-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAKIIDGKT IAQQVRSEVA QKVQARIAAG LRAPGLAVVL VGSNPASQIY
60 70 80 90 100
VASKRKACEE VGFVSRSYDL PETTSEAELL ELIDTLNADN TIDGILVQLP
110 120 130 140 150
LPAGIDNVKV LERIHPDKDV DGFHPYNVGR LCQRAPRLRP CTPRGIVTLL
160 170 180 190 200
ERYNIDTFGL NAVVIGASNI VGRPMSMELL LAGCTTTVTH RFTKNLRHHV
210 220 230 240 250
ENADLLIVAV GKPGFIPGDW IKEGAIVIDV GINRLENGKV VGDVVFEDAA
260 270 280
KRASYITPVP GGVGPMTVAT LIENTLQACV EYHDPQDE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 47 | S → L in AAB40282 (Ref. 3) Curated | 1 | |
Sequence conflicti | 200 | V → L in AAA23803 (PubMed:1748668).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74789 Genomic DNA Translation: AAA23803.1 D10588 Genomic DNA Translation: BAA01445.1 U82664 Genomic DNA Translation: AAB40282.1 U00096 Genomic DNA Translation: AAC73631.1 AP009048 Genomic DNA Translation: BAE76306.1 |
PIRi | H64784, JS0662 |
RefSeqi | NP_415062.1, NC_000913.3 WP_000729160.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73631; AAC73631; b0529 BAE76306; BAE76306; BAE76306 |
GeneIDi | 945221 |
KEGGi | ecj:JW0518 eco:b0529 |
PATRICi | fig|1411691.4.peg.1749 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74789 Genomic DNA Translation: AAA23803.1 D10588 Genomic DNA Translation: BAA01445.1 U82664 Genomic DNA Translation: AAB40282.1 U00096 Genomic DNA Translation: AAC73631.1 AP009048 Genomic DNA Translation: BAE76306.1 |
PIRi | H64784, JS0662 |
RefSeqi | NP_415062.1, NC_000913.3 WP_000729160.1, NZ_STEB01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1B0A | X-ray | 2.56 | A | 1-288 | [»] | |
5O22 | X-ray | 2.10 | A/B/C/D | 2-288 | [»] | |
5O28 | X-ray | 1.89 | A/B/C/D | 2-288 | [»] | |
5O2A | X-ray | 1.90 | A/B/C/D | 2-288 | [»] | |
SMRi | P24186 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262819, 297 interactors |
DIPi | DIP-9675N |
IntActi | P24186, 4 interactors |
STRINGi | 511145.b0529 |
Proteomic databases
jPOSTi | P24186 |
PaxDbi | P24186 |
PRIDEi | P24186 |
Genome annotation databases
EnsemblBacteriai | AAC73631; AAC73631; b0529 BAE76306; BAE76306; BAE76306 |
GeneIDi | 945221 |
KEGGi | ecj:JW0518 eco:b0529 |
PATRICi | fig|1411691.4.peg.1749 |
Organism-specific databases
EchoBASEi | EB0324 |
Phylogenomic databases
eggNOGi | COG0190, Bacteria |
HOGENOMi | CLU_034045_2_1_6 |
InParanoidi | P24186 |
PhylomeDBi | P24186 |
Enzyme and pathway databases
UniPathwayi | UPA00193 |
BioCyci | EcoCyc:FOLD-MONOMER |
BRENDAi | 1.5.1.5, 2026 3.5.4.9, 2026 |
SABIO-RKi | P24186 |
Miscellaneous databases
EvolutionaryTracei | P24186 |
PROi | PR:P24186 |
Family and domain databases
CDDi | cd01080, NAD_bind_m-THF_DH_Cyclohyd, 1 hit |
HAMAPi | MF_01576, THF_DHG_CYH, 1 hit |
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR000672, THF_DH/CycHdrlase IPR020630, THF_DH/CycHdrlase_cat_dom IPR020867, THF_DH/CycHdrlase_CS IPR020631, THF_DH/CycHdrlase_NAD-bd_dom |
Pfami | View protein in Pfam PF00763, THF_DHG_CYH, 1 hit PF02882, THF_DHG_CYH_C, 1 hit |
PRINTSi | PR00085, THFDHDRGNASE |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00766, THF_DHG_CYH_1, 1 hit PS00767, THF_DHG_CYH_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FOLD_ECOLI | |
Accessioni | P24186Primary (citable) accession number: P24186 Secondary accession number(s): P77132, Q2MBQ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 167 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families