UniProtKB - P24183 (FDNG_ECOLI)
Protein
Formate dehydrogenase, nitrate-inducible, major subunit
Gene
fdnG
Organism
Escherichia coli (strain K12)
Status
Functioni
Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit FdnG contains the formate oxidation site. Electrons are transferred from formate to menaquinone in the gamma subunit (FdnI), through the 4Fe-4S clusters in the beta subunit (FdnH). Formate dehydrogenase-N is part of a system that generates proton motive force, together with the dissimilatory nitrate reductase (Nar).1 Publication
Catalytic activityi
- EC:1.17.5.31 Publication
Cofactori
Protein has several cofactor binding sites:- Mo-bis(molybdopterin guanine dinucleotide)1 PublicationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.1 Publication
- [4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 50 | Iron-sulfur (4Fe-4S) | 1 | |
Metal bindingi | 53 | Iron-sulfur (4Fe-4S) | 1 | |
Metal bindingi | 57 | Iron-sulfur (4Fe-4S) | 1 | |
Metal bindingi | 92 | Iron-sulfur (4Fe-4S) | 1 | |
Metal bindingi | 196 | Molybdenum | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: EcoCyc
- electron transfer activity Source: EcoCyc
- formate dehydrogenase (cytochrome-c-553) activity Source: InterPro
- formate dehydrogenase (NAD+) activity Source: InterPro
- formate dehydrogenase (quinone) activity Source: EcoCyc
- molybdenum ion binding Source: EcoCyc
- molybdopterin cofactor binding Source: InterPro
- selenium binding Source: EcoCyc
GO - Biological processi
- anaerobic respiration Source: EcoCyc
- formate oxidation Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:FDNG-MONOMER MetaCyc:FDNG-MONOMER |
BRENDAi | 1.1.5.6, 2026 |
Protein family/group databases
TCDBi | 5.A.3.2.1, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family |
Names & Taxonomyi
Protein namesi | Recommended name: Formate dehydrogenase, nitrate-inducible, major subunit (EC:1.17.5.31 Publication)Alternative name(s): Anaerobic formate dehydrogenase major subunit Formate dehydrogenase-N subunit alpha Short name: FDH-N subunit alpha |
Gene namesi | Name:fdnG Ordered Locus Names:b1474, JW1470 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm 1 Publication
Other locations
- formate dehydrogenase complex Source: EcoCyc
- membrane Source: UniProtKB
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 33 | Tat-type signalPROSITE-ProRule annotationAdd BLAST | 33 | |
ChainiPRO_0000063222 | 34 – 1015 | Formate dehydrogenase, nitrate-inducible, major subunitAdd BLAST | 982 |
Post-translational modificationi
Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Proteomic databases
jPOSTi | P24183 |
PaxDbi | P24183 |
PRIDEi | P24183 |
Expressioni
Inductioni
By nitrate under anaerobic conditions.1 Publication
Interactioni
Subunit structurei
Trimer of heterotrimers, consisting of subunits alpha, beta and gamma.
1 PublicationBinary interactionsi
P24183
With | #Exp. | IntAct |
---|---|---|
fdhE [P13024] | 6 | EBI-550115,EBI-550129 |
Protein-protein interaction databases
BioGRIDi | 4262900, 30 interactors 850396, 1 interactor |
ComplexPortali | CPX-1975, Formate dehydrogenase N complex |
DIPi | DIP-9573N |
IntActi | P24183, 15 interactors |
STRINGi | 511145.b1474 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P24183 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24183 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 43 – 106 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 64 |
Sequence similaritiesi
Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0243, Bacteria COG3383, Bacteria |
InParanoidi | P24183 |
PhylomeDBi | P24183 |
Family and domain databases
InterProi | View protein in InterPro IPR009010, Asp_de-COase-like_dom_sf IPR006443, Formate-DH-alph_fdnG IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR006655, Mopterin_OxRdtase_prok_CS IPR027467, MopterinOxRdtase_cofactor_BS IPR006311, TAT_signal |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF01568, Molydop_binding, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit |
TIGRFAMsi | TIGR01553, formate-DH-alph, 1 hit |
PROSITEi | View protein in PROSITE PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00551, MOLYBDOPTERIN_PROK_1, 1 hit PS00932, MOLYBDOPTERIN_PROK_3, 1 hit PS51318, TAT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P24183-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVSRRQFFK ICAGGMAGTT VAALGFAPKQ ALAQARNYKL LRAKEIRNTC
60 70 80 90 100
TYCSVGCGLL MYSLGDGAKN AREAIYHIEG DPDHPVSRGA LCPKGAGLLD
110 120 130 140 150
YVNSENRLRY PEYRAPGSDK WQRISWEEAF SRIAKLMKAD RDANFIEKNE
160 170 180 190 200
QGVTVNRWLS TGMLCASGAS NETGMLTQKF ARSLGMLAVD NQARVUHGPT
210 220 230 240 250
VASLAPTFGR GAMTNHWVDI KNANVVMVMG GNAAEAHPVG FRWAMEAKNN
260 270 280 290 300
NDATLIVVDP RFTRTASVAD IYAPIRSGTD ITFLSGVLRY LIENNKINAE
310 320 330 340 350
YVKHYTNASL LVRDDFAFED GLFSGYDAEK RQYDKSSWNY QLDENGYAKR
360 370 380 390 400
DETLTHPRCV WNLLKEHVSR YTPDVVENIC GTPKADFLKV CEVLASTSAP
410 420 430 440 450
DRTTTFLYAL GWTQHTVGAQ NIRTMAMIQL LLGNMGMAGG GVNALRGHSN
460 470 480 490 500
IQGLTDLGLL STSLPGYLTL PSEKQVDLQS YLEANTPKAT LADQVNYWSN
510 520 530 540 550
YPKFFVSLMK SFYGDAAQKE NNWGYDWLPK WDQTYDVIKY FNMMDEGKVT
560 570 580 590 600
GYFCQGFNPV ASFPDKNKVV SCLSKLKYMV VIDPLVTETS TFWQNHGESN
610 620 630 640 650
DVDPASIQTE VFRLPSTCFA EEDGSIANSG RWLQWHWKGQ DAPGEARNDG
660 670 680 690 700
EILAGIYHHL RELYQSEGGK GVEPLMKMSW NYKQPHEPQS DEVAKENNGY
710 720 730 740 750
ALEDLYDANG VLIAKKGQLL SSFAHLRDDG TTASSCWIYT GSWTEQGNQM
760 770 780 790 800
ANRDNSDPSG LGNTLGWAWA WPLNRRVLYN RASADINGKP WDPKRMLIQW
810 820 830 840 850
NGSKWTGNDI PDFGNAAPGT PTGPFIMQPE GMGRLFAINK MAEGPFPEHY
860 870 880 890 900
EPIETPLGTN PLHPNVVSNP VVRLYEQDAL RMGKKEQFPY VGTTYRLTEH
910 920 930 940 950
FHTWTKHALL NAIAQPEQFV EISETLAAAK GINNGDRVTV SSKRGFIRAV
960 970 980 990 1000
AVVTRRLKPL NVNGQQVETV GIPIHWGFEG VARKGYIANT LTPNVGDANS
1010
QTPEYKAFLV NIEKA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 96 | A → P (PubMed:1834669).Curated | 1 | |
Sequence conflicti | 484 – 491 | ANTPKATL → GEHAERRRW (PubMed:1834669).Curated | 8 | |
Sequence conflicti | 941 | S → A (PubMed:1834669).Curated | 1 |
Non-standard residue
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-standard residuei | 196 | Selenocysteine | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M75029 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAD13438.1 AP009048 Genomic DNA Translation: BAA15123.1 |
PIRi | E64900, JS0628 |
RefSeqi | NP_415991.1, NC_000913.3 WP_010723100.1, NZ_LN832404.1 |
Genome annotation databases
GeneIDi | 946035 |
KEGGi | ecj:JW1470 eco:b1474 |
PATRICi | fig|511145.12.peg.1540 |
Keywords - Coding sequence diversityi
SelenocysteineSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M75029 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAD13438.1 AP009048 Genomic DNA Translation: BAA15123.1 |
PIRi | E64900, JS0628 |
RefSeqi | NP_415991.1, NC_000913.3 WP_010723100.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KQF | X-ray | 1.60 | A | 1-1015 | [»] | |
1KQG | X-ray | 2.80 | A | 1-1015 | [»] | |
SMRi | P24183 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262900, 30 interactors 850396, 1 interactor |
ComplexPortali | CPX-1975, Formate dehydrogenase N complex |
DIPi | DIP-9573N |
IntActi | P24183, 15 interactors |
STRINGi | 511145.b1474 |
Chemistry databases
DrugBanki | DB07918, 2-heptyl-4-hydroxyquinoline N-oxide |
Protein family/group databases
TCDBi | 5.A.3.2.1, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family |
Proteomic databases
jPOSTi | P24183 |
PaxDbi | P24183 |
PRIDEi | P24183 |
Genome annotation databases
GeneIDi | 946035 |
KEGGi | ecj:JW1470 eco:b1474 |
PATRICi | fig|511145.12.peg.1540 |
Organism-specific databases
EchoBASEi | EB1209 |
Phylogenomic databases
eggNOGi | COG0243, Bacteria COG3383, Bacteria |
InParanoidi | P24183 |
PhylomeDBi | P24183 |
Enzyme and pathway databases
BioCyci | EcoCyc:FDNG-MONOMER MetaCyc:FDNG-MONOMER |
BRENDAi | 1.1.5.6, 2026 |
Miscellaneous databases
EvolutionaryTracei | P24183 |
PROi | PR:P24183 |
Family and domain databases
InterProi | View protein in InterPro IPR009010, Asp_de-COase-like_dom_sf IPR006443, Formate-DH-alph_fdnG IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR006655, Mopterin_OxRdtase_prok_CS IPR027467, MopterinOxRdtase_cofactor_BS IPR006311, TAT_signal |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF01568, Molydop_binding, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit |
TIGRFAMsi | TIGR01553, formate-DH-alph, 1 hit |
PROSITEi | View protein in PROSITE PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00551, MOLYBDOPTERIN_PROK_1, 1 hit PS00932, MOLYBDOPTERIN_PROK_3, 1 hit PS51318, TAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FDNG_ECOLI | |
Accessioni | P24183Primary (citable) accession number: P24183 Secondary accession number(s): P78261 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | February 26, 2008 | |
Last modified: | April 7, 2021 | |
This is version 192 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families