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UniProtKB - P24182 (ACCC_ECOLI)
Protein
Biotin carboxylase
Gene
accC
Organism
Escherichia coli (strain K12)
Status
Functioni
This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.
Catalytic activityi
- ATP + hydrogencarbonate + N6-biotinyl-L-lysyl-[protein] = ADP + H+ + N6-carboxybiotinyl-L-lysyl-[protein] + phosphateEC:6.3.4.14
- EC:6.4.1.2
: malonyl-CoA biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes malonyl-CoA from acetyl-CoA. This subpathway is part of the pathway malonyl-CoA biosynthesis, which is itself part of Lipid metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes malonyl-CoA from acetyl-CoA, the pathway malonyl-CoA biosynthesis and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 116 | ATP1 Publication | 1 | |
Binding sitei | 201 | ATP1 Publication | 1 | |
Binding sitei | 236 | ATP1 Publication | 1 | |
Active sitei | 292 | Sequence analysis | 1 |
GO - Molecular functioni
- acetyl-CoA carboxylase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- biotin carboxylase activity Source: EcoliWiki
- metal ion binding Source: InterPro
GO - Biological processi
- fatty acid biosynthetic process Source: ComplexPortal
- malonyl-CoA biosynthetic process Source: ComplexPortal
- negative regulation of fatty acid biosynthetic process Source: EcoliWiki
Keywordsi
Molecular function | Ligase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Ligand | ATP-binding, Biotin, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:BIOTIN-CARBOXYL-MONOMER |
BRENDAi | 6.3.4.14, 2026 |
SABIO-RKi | P24182 |
UniPathwayi | UPA00655;UER00711 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:accC Synonyms:fabG Ordered Locus Names:b3256, JW3224 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: EcoCyc
Other locations
- acetyl-CoA carboxylase complex Source: ComplexPortal
- cytoplasm Source: EcoliWiki
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 19 | R → E: Loss of homodimerization. No effect on ATP binding. 1 Publication | 1 | |
Mutagenesisi | 23 | E → R: Loss of homodimerization. No effect on ATP binding. 1 Publication | 1 | |
Mutagenesisi | 363 | F → A: Loss of homodimerization. No effect on ATP binding. 1 Publication | 1 | |
Mutagenesisi | 366 | R → E: Loss of homodimerization. No effect on ATP binding. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB08314, (2-AMINO-1,3-OXAZOL-5-YL)-(3-BROMOPHENYL)METHANONE DB08315, 2-AMINO-N,N-BIS(PHENYLMETHYL)-1,3-OXAZOLE-5-CARBOXAMIDE DB08074, 3-(3-Methyl-2-buten-1-yl)-3H-purin-6-amine DB08075, 4-(2-amino-1,3-thiazol-4-yl)pyrimidin-2-amine DB08076, 4-[1-(2,6-dichlorobenzyl)-2-methyl-1H-imidazol-4-yl]pyrimidin-2-amine DB08316, 4-amino-7,7-dimethyl-7,8-dihydroquinazolin-5(6H)-one DB08317, 5-methyl-6-phenylquinazoline-2,4-diamine DB08144, 6-(2,6-dibromophenyl)pyrido[2,3-d]pyrimidine-2,7-diamine DB08145, 6-(2,6-DIMETHOXYPHENYL)PYRIDO[2,3-D]PYRIMIDINE-2,7-DIAMINE DB08318, 6-(2-phenoxyethoxy)-1,3,5-triazine-2,4-diamine DB08146, 7-(2,5-dihydropyrrol-1-yl)-6-phenyl-pyrido[6,5-d]pyrimidin-2-amine |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000146791 | 1 – 449 | Biotin carboxylaseAdd BLAST | 449 |
Proteomic databases
jPOSTi | P24182 |
PaxDbi | P24182 |
PRIDEi | P24182 |
Interactioni
Subunit structurei
Acetyl-CoA carboxylase is a heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and the two subunits of carboxyl transferase in a 2:2 complex.
2 PublicationsBinary interactionsi
P24182
With | #Exp. | IntAct |
---|---|---|
accB [P0ABD8] | 10 | EBI-542308,EBI-542320 |
accD [P0A9Q5] | 4 | EBI-542308,EBI-542064 |
cysM [P16703] | 3 | EBI-542308,EBI-542376 |
ribD [P25539] | 3 | EBI-542308,EBI-552457 |
Protein-protein interaction databases
BioGRIDi | 4262456, 269 interactors 852073, 6 interactors |
ComplexPortali | CPX-3206, Acetyl-CoA carboxylase complex |
DIPi | DIP-9035N |
IntActi | P24182, 20 interactors |
STRINGi | 511145.b3256 |
Chemistry databases
BindingDBi | P24182 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P24182 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24182 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 445 | Biotin carboxylationAdd BLAST | 445 | |
Domaini | 120 – 317 | ATP-graspPROSITE-ProRule annotationAdd BLAST | 198 |
Phylogenomic databases
eggNOGi | COG0439, Bacteria |
HOGENOMi | CLU_000395_3_2_6 |
InParanoidi | P24182 |
PhylomeDBi | P24182 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
InterProi | View protein in InterPro IPR004549, Acetyl_CoA_COase_biotin_COase IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR005481, BC-like_N IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR016185, PreATP-grasp_dom_sf IPR011054, Rudment_hybrid_motif |
Pfami | View protein in Pfam PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF02786, CPSase_L_D2, 1 hit |
SMARTi | View protein in SMART SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR00514, accC, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS00866, CPSASE_1, 1 hit PS00867, CPSASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P24182-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLDKIVIANR GEIALRILRA CKELGIKTVA VHSSADRDLK HVLLADETVC
60 70 80 90 100
IGPAPSVKSY LNIPAIISAA EITGAVAIHP GYGFLSENAN FAEQVERSGF
110 120 130 140 150
IFIGPKAETI RLMGDKVSAI AAMKKAGVPC VPGSDGPLGD DMDKNRAIAK
160 170 180 190 200
RIGYPVIIKA SGGGGGRGMR VVRGDAELAQ SISMTRAEAK AAFSNDMVYM
210 220 230 240 250
EKYLENPRHV EIQVLADGQG NAIYLAERDC SMQRRHQKVV EEAPAPGITP
260 270 280 290 300
ELRRYIGERC AKACVDIGYR GAGTFEFLFE NGEFYFIEMN TRIQVEHPVT
310 320 330 340 350
EMITGVDLIK EQLRIAAGQP LSIKQEEVHV RGHAVECRIN AEDPNTFLPS
360 370 380 390 400
PGKITRFHAP GGFGVRWESH IYAGYTVPPY YDSMIGKLIC YGENRDVAIA
410 420 430 440
RMKNALQELI IDGIKTNVDL QIRIMNDENF QHGGTNIHYL EKKLGLQEK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 136 | G → A (PubMed:2575489).Curated | 1 | |
Sequence conflicti | 160 | A → P (PubMed:2575489).Curated | 1 | |
Sequence conflicti | 260 – 261 | CA → SR (PubMed:1370469).Curated | 2 | |
Sequence conflicti | 313 | L → M in AAA23748 (PubMed:1682920).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M79446 Genomic DNA Translation: AAA23748.1 M80458 Genomic DNA Translation: AAA23409.1 M83198 Genomic DNA Translation: AAA23746.1 U18997 Genomic DNA Translation: AAA58059.1 U00096 Genomic DNA Translation: AAC76288.1 AP009048 Genomic DNA Translation: BAE77297.1 M32214 Genomic DNA No translation available. |
PIRi | JS0632 |
RefSeqi | NP_417722.1, NC_000913.3 WP_000884639.1, NZ_STEB01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC76288; AAC76288; b3256 BAE77297; BAE77297; BAE77297 |
GeneIDi | 58460262 947761 |
KEGGi | ecj:JW3224 eco:b3256 |
PATRICi | fig|511145.12.peg.3355 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M79446 Genomic DNA Translation: AAA23748.1 M80458 Genomic DNA Translation: AAA23409.1 M83198 Genomic DNA Translation: AAA23746.1 U18997 Genomic DNA Translation: AAA58059.1 U00096 Genomic DNA Translation: AAC76288.1 AP009048 Genomic DNA Translation: BAE77297.1 M32214 Genomic DNA No translation available. |
PIRi | JS0632 |
RefSeqi | NP_417722.1, NC_000913.3 WP_000884639.1, NZ_STEB01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BNC | X-ray | 2.40 | A/B | 1-449 | [»] | |
1DV1 | X-ray | 1.90 | A/B | 1-449 | [»] | |
1DV2 | X-ray | 2.50 | A/B | 1-449 | [»] | |
2GPS | X-ray | 2.80 | A/B | 1-449 | [»] | |
2GPW | X-ray | 2.20 | A/B/C/D | 1-449 | [»] | |
2J9G | X-ray | 2.05 | A/B | 1-449 | [»] | |
2V58 | X-ray | 2.10 | A/B | 1-449 | [»] | |
2V59 | X-ray | 2.40 | A/B | 1-449 | [»] | |
2V5A | X-ray | 2.31 | A/B | 1-449 | [»] | |
2VR1 | X-ray | 2.60 | A/B | 1-449 | [»] | |
2W6M | X-ray | 2.00 | A/B | 1-449 | [»] | |
2W6N | X-ray | 1.87 | A/B | 1-449 | [»] | |
2W6O | X-ray | 2.50 | A/C | 1-449 | [»] | |
2W6P | X-ray | 1.85 | A/B | 1-449 | [»] | |
2W6Q | X-ray | 2.05 | A/B | 1-449 | [»] | |
2W6Z | X-ray | 1.90 | A/B | 1-449 | [»] | |
2W70 | X-ray | 1.77 | A/B | 1-449 | [»] | |
2W71 | X-ray | 1.99 | A/C | 1-449 | [»] | |
3G8C | X-ray | 2.00 | A/B | 1-444 | [»] | |
3G8D | X-ray | 1.90 | A/B | 1-444 | [»] | |
3JZF | X-ray | 2.13 | A/B | 1-449 | [»] | |
3JZI | X-ray | 2.31 | A/B | 1-449 | [»] | |
3RUP | X-ray | 1.99 | A/B | 1-449 | [»] | |
3RV3 | X-ray | 1.91 | A/B | 1-449 | [»] | |
3RV4 | X-ray | 1.98 | A | 1-449 | [»] | |
4HR7 | X-ray | 2.50 | A/C/E/F | 1-449 | [»] | |
6OI9 | X-ray | 2.06 | A/B | 1-449 | [»] | |
SMRi | P24182 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262456, 269 interactors 852073, 6 interactors |
ComplexPortali | CPX-3206, Acetyl-CoA carboxylase complex |
DIPi | DIP-9035N |
IntActi | P24182, 20 interactors |
STRINGi | 511145.b3256 |
Chemistry databases
BindingDBi | P24182 |
DrugBanki | DB08314, (2-AMINO-1,3-OXAZOL-5-YL)-(3-BROMOPHENYL)METHANONE DB08315, 2-AMINO-N,N-BIS(PHENYLMETHYL)-1,3-OXAZOLE-5-CARBOXAMIDE DB08074, 3-(3-Methyl-2-buten-1-yl)-3H-purin-6-amine DB08075, 4-(2-amino-1,3-thiazol-4-yl)pyrimidin-2-amine DB08076, 4-[1-(2,6-dichlorobenzyl)-2-methyl-1H-imidazol-4-yl]pyrimidin-2-amine DB08316, 4-amino-7,7-dimethyl-7,8-dihydroquinazolin-5(6H)-one DB08317, 5-methyl-6-phenylquinazoline-2,4-diamine DB08144, 6-(2,6-dibromophenyl)pyrido[2,3-d]pyrimidine-2,7-diamine DB08145, 6-(2,6-DIMETHOXYPHENYL)PYRIDO[2,3-D]PYRIMIDINE-2,7-DIAMINE DB08318, 6-(2-phenoxyethoxy)-1,3,5-triazine-2,4-diamine DB08146, 7-(2,5-dihydropyrrol-1-yl)-6-phenyl-pyrido[6,5-d]pyrimidin-2-amine |
Proteomic databases
jPOSTi | P24182 |
PaxDbi | P24182 |
PRIDEi | P24182 |
Genome annotation databases
EnsemblBacteriai | AAC76288; AAC76288; b3256 BAE77297; BAE77297; BAE77297 |
GeneIDi | 58460262 947761 |
KEGGi | ecj:JW3224 eco:b3256 |
PATRICi | fig|511145.12.peg.3355 |
Organism-specific databases
EchoBASEi | EB0272 |
Phylogenomic databases
eggNOGi | COG0439, Bacteria |
HOGENOMi | CLU_000395_3_2_6 |
InParanoidi | P24182 |
PhylomeDBi | P24182 |
Enzyme and pathway databases
UniPathwayi | UPA00655;UER00711 |
BioCyci | EcoCyc:BIOTIN-CARBOXYL-MONOMER |
BRENDAi | 6.3.4.14, 2026 |
SABIO-RKi | P24182 |
Miscellaneous databases
EvolutionaryTracei | P24182 |
PROi | PR:P24182 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
InterProi | View protein in InterPro IPR004549, Acetyl_CoA_COase_biotin_COase IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR005481, BC-like_N IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR016185, PreATP-grasp_dom_sf IPR011054, Rudment_hybrid_motif |
Pfami | View protein in Pfam PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF02786, CPSase_L_D2, 1 hit |
SMARTi | View protein in SMART SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR00514, accC, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS00866, CPSASE_1, 1 hit PS00867, CPSASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACCC_ECOLI | |
Accessioni | P24182Primary (citable) accession number: P24182 Secondary accession number(s): Q2M8V9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 200 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references