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Protein

Biotin carboxylase

Gene

accC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.

Catalytic activityi

ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine.
ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA.

Pathwayi: malonyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes malonyl-CoA from acetyl-CoA.
Proteins known to be involved in this subpathway in this organism are:
  1. Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD), Biotin carboxylase (accC), Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA)
This subpathway is part of the pathway malonyl-CoA biosynthesis, which is itself part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes malonyl-CoA from acetyl-CoA, the pathway malonyl-CoA biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116ATP1 Publication1
Binding sitei201ATP1 Publication1
Binding sitei236ATP1 Publication1
Active sitei292Sequence analysis1

GO - Molecular functioni

GO - Biological processi

  • fatty acid biosynthetic process Source: EcoCyc
  • malonyl-CoA biosynthetic process Source: UniProtKB-UniPathway
  • negative regulation of fatty acid biosynthetic process Source: EcoliWiki

Keywordsi

Molecular functionLigase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandATP-binding, Biotin, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:BIOTIN-CARBOXYL-MONOMER
MetaCyc:BIOTIN-CARBOXYL-MONOMER
BRENDAi6.3.4.14 2026
SABIO-RKiP24182
UniPathwayi
UPA00655;UER00711

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin carboxylase (EC:6.3.4.14)
Alternative name(s):
Acetyl-CoA carboxylase subunit A (EC:6.4.1.2)
Short name:
ACC
Gene namesi
Name:accC
Synonyms:fabG
Ordered Locus Names:b3256, JW3224
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10276 accC

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19R → E: Loss of homodimerization. No effect on ATP binding. 1 Publication1
Mutagenesisi23E → R: Loss of homodimerization. No effect on ATP binding. 1 Publication1
Mutagenesisi363F → A: Loss of homodimerization. No effect on ATP binding. 1 Publication1
Mutagenesisi366R → E: Loss of homodimerization. No effect on ATP binding. 1 Publication1

Chemistry databases

DrugBankiDB08074 3-(3-methylbut-2-en-1-yl)-3H-purin-6-amine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001467911 – 449Biotin carboxylaseAdd BLAST449

Proteomic databases

EPDiP24182
PaxDbiP24182
PRIDEiP24182

Interactioni

Subunit structurei

Acetyl-CoA carboxylase is a heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and the two subunits of carboxyl transferase in a 2:2 complex.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi4262456, 269 interactors
ComplexPortaliCPX-3206 Acetyl-CoA carboxylase complex
DIPiDIP-9035N
IntActiP24182, 20 interactors
STRINGi316385.ECDH10B_3431

Chemistry databases

BindingDBiP24182

Structurei

Secondary structure

1449
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP24182
SMRiP24182
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24182

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 445Biotin carboxylationAdd BLAST445
Domaini120 – 317ATP-graspPROSITE-ProRule annotationAdd BLAST198

Phylogenomic databases

eggNOGiENOG4105CER Bacteria
COG0439 LUCA
HOGENOMiHOG000008988
InParanoidiP24182
KOiK01961
PhylomeDBiP24182

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
InterProiView protein in InterPro
IPR004549 Acetyl_CoA_COase_biotin_COase
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR005481 BC-like_N
IPR011764 Biotin_carboxylation_dom
IPR005482 Biotin_COase_C
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR016185 PreATP-grasp_dom_sf
IPR011054 Rudment_hybrid_motif
PfamiView protein in Pfam
PF02785 Biotin_carb_C, 1 hit
PF00289 Biotin_carb_N, 1 hit
PF02786 CPSase_L_D2, 1 hit
SMARTiView protein in SMART
SM00878 Biotin_carb_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR00514 accC, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS50979 BC, 1 hit
PS00866 CPSASE_1, 1 hit
PS00867 CPSASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P24182-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLDKIVIANR GEIALRILRA CKELGIKTVA VHSSADRDLK HVLLADETVC
60 70 80 90 100
IGPAPSVKSY LNIPAIISAA EITGAVAIHP GYGFLSENAN FAEQVERSGF
110 120 130 140 150
IFIGPKAETI RLMGDKVSAI AAMKKAGVPC VPGSDGPLGD DMDKNRAIAK
160 170 180 190 200
RIGYPVIIKA SGGGGGRGMR VVRGDAELAQ SISMTRAEAK AAFSNDMVYM
210 220 230 240 250
EKYLENPRHV EIQVLADGQG NAIYLAERDC SMQRRHQKVV EEAPAPGITP
260 270 280 290 300
ELRRYIGERC AKACVDIGYR GAGTFEFLFE NGEFYFIEMN TRIQVEHPVT
310 320 330 340 350
EMITGVDLIK EQLRIAAGQP LSIKQEEVHV RGHAVECRIN AEDPNTFLPS
360 370 380 390 400
PGKITRFHAP GGFGVRWESH IYAGYTVPPY YDSMIGKLIC YGENRDVAIA
410 420 430 440
RMKNALQELI IDGIKTNVDL QIRIMNDENF QHGGTNIHYL EKKLGLQEK
Length:449
Mass (Da):49,321
Last modified:February 1, 1994 - v2
Checksum:i68C55F10ACB4F170
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136G → A (PubMed:2575489).Curated1
Sequence conflicti160A → P (PubMed:2575489).Curated1
Sequence conflicti260 – 261CA → SR (PubMed:1370469).Curated2
Sequence conflicti313L → M in AAA23748 (PubMed:1682920).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M79446 Genomic DNA Translation: AAA23748.1
M80458 Genomic DNA Translation: AAA23409.1
M83198 Genomic DNA Translation: AAA23746.1
U18997 Genomic DNA Translation: AAA58059.1
U00096 Genomic DNA Translation: AAC76288.1
AP009048 Genomic DNA Translation: BAE77297.1
M32214 Genomic DNA No translation available.
PIRiJS0632
RefSeqiNP_417722.1, NC_000913.3
WP_000884639.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76288; AAC76288; b3256
BAE77297; BAE77297; BAE77297
GeneIDi947761
KEGGiecj:JW3224
eco:b3256
PATRICifig|511145.12.peg.3355

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M79446 Genomic DNA Translation: AAA23748.1
M80458 Genomic DNA Translation: AAA23409.1
M83198 Genomic DNA Translation: AAA23746.1
U18997 Genomic DNA Translation: AAA58059.1
U00096 Genomic DNA Translation: AAC76288.1
AP009048 Genomic DNA Translation: BAE77297.1
M32214 Genomic DNA No translation available.
PIRiJS0632
RefSeqiNP_417722.1, NC_000913.3
WP_000884639.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BNCX-ray2.40A/B1-449[»]
1DV1X-ray1.90A/B1-449[»]
1DV2X-ray2.50A/B1-449[»]
1K69model-A1-447[»]
2GPSX-ray2.80A/B1-449[»]
2GPWX-ray2.20A/B/C/D1-449[»]
2J9GX-ray2.05A/B1-449[»]
2V58X-ray2.10A/B1-449[»]
2V59X-ray2.40A/B1-449[»]
2V5AX-ray2.31A/B1-449[»]
2VR1X-ray2.60A/B1-449[»]
2W6MX-ray2.00A/B1-449[»]
2W6NX-ray1.87A/B1-449[»]
2W6OX-ray2.50A/C1-449[»]
2W6PX-ray1.85A/B1-449[»]
2W6QX-ray2.05A/B1-449[»]
2W6ZX-ray1.90A/B1-449[»]
2W70X-ray1.77A/B1-449[»]
2W71X-ray1.99A/C1-449[»]
3G8CX-ray2.00A/B1-444[»]
3G8DX-ray1.90A/B1-444[»]
3JZFX-ray2.13A/B1-449[»]
3JZIX-ray2.31A/B1-449[»]
3RUPX-ray1.99A/B1-449[»]
3RV3X-ray1.91A/B1-449[»]
3RV4X-ray1.98A1-449[»]
4HR7X-ray2.50A/C/E/F1-449[»]
ProteinModelPortaliP24182
SMRiP24182
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262456, 269 interactors
ComplexPortaliCPX-3206 Acetyl-CoA carboxylase complex
DIPiDIP-9035N
IntActiP24182, 20 interactors
STRINGi316385.ECDH10B_3431

Chemistry databases

BindingDBiP24182
DrugBankiDB08074 3-(3-methylbut-2-en-1-yl)-3H-purin-6-amine

Proteomic databases

EPDiP24182
PaxDbiP24182
PRIDEiP24182

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76288; AAC76288; b3256
BAE77297; BAE77297; BAE77297
GeneIDi947761
KEGGiecj:JW3224
eco:b3256
PATRICifig|511145.12.peg.3355

Organism-specific databases

EchoBASEiEB0272
EcoGeneiEG10276 accC

Phylogenomic databases

eggNOGiENOG4105CER Bacteria
COG0439 LUCA
HOGENOMiHOG000008988
InParanoidiP24182
KOiK01961
PhylomeDBiP24182

Enzyme and pathway databases

UniPathwayi
UPA00655;UER00711

BioCyciEcoCyc:BIOTIN-CARBOXYL-MONOMER
MetaCyc:BIOTIN-CARBOXYL-MONOMER
BRENDAi6.3.4.14 2026
SABIO-RKiP24182

Miscellaneous databases

EvolutionaryTraceiP24182
PROiPR:P24182

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
InterProiView protein in InterPro
IPR004549 Acetyl_CoA_COase_biotin_COase
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR005481 BC-like_N
IPR011764 Biotin_carboxylation_dom
IPR005482 Biotin_COase_C
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR016185 PreATP-grasp_dom_sf
IPR011054 Rudment_hybrid_motif
PfamiView protein in Pfam
PF02785 Biotin_carb_C, 1 hit
PF00289 Biotin_carb_N, 1 hit
PF02786 CPSase_L_D2, 1 hit
SMARTiView protein in SMART
SM00878 Biotin_carb_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR00514 accC, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS50979 BC, 1 hit
PS00866 CPSASE_1, 1 hit
PS00867 CPSASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACCC_ECOLI
AccessioniPrimary (citable) accession number: P24182
Secondary accession number(s): Q2M8V9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 1, 1994
Last modified: November 7, 2018
This is version 181 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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