UniProtKB - P24180 (ACRE_ECOLI)
Protein
Multidrug export protein AcrE
Gene
acrE
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the tripartite efflux system AcrEF-TolC. Involved in the efflux of indole and organic solvents.2 Publications
GO - Molecular functioni
- xenobiotic transmembrane transporter activity Source: GO_Central
GO - Biological processi
- response to antibiotic Source: GO_Central
- response to drug Source: EcoliWiki
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10266-MONOMER MetaCyc:EG10266-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Multidrug export protein AcrEAlternative name(s): Acriflavine resistance protein E Protein EnvC |
Gene namesi | Name:acrE Synonyms:envC Ordered Locus Names:b3265, JW3233 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Lipid-anchor 1 Publication
GO - Cellular componenti
- intrinsic component of plasma membrane Source: EcoliWiki
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | PROSITE-ProRule annotationAdd BLAST | 23 | |
ChainiPRO_0000018688 | 24 – 385 | Multidrug export protein AcrEAdd BLAST | 362 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 24 | N-palmitoyl cysteinePROSITE-ProRule annotation | 1 | |
Lipidationi | 24 | S-diacylglycerol cysteinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
PaxDbi | P24180 |
PRIDEi | P24180 |
Expressioni
Inductioni
Induced by LeuO, part of the acrEF operon.1 Publication
Interactioni
Subunit structurei
Part of the tripartite efflux system AcrEF-TolC, which is composed of an inner membrane transporter, AcrF, a periplasmic membrane fusion protein, AcrE, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes (Probable).
1 PublicationProtein-protein interaction databases
BioGRIDi | 4261953, 367 interactors |
ComplexPortali | CPX-4265, AcrEF-TolC multidrug efflux transport complex |
STRINGi | 511145.b3265 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0845, Bacteria |
HOGENOMi | CLU_018816_2_1_6 |
InParanoidi | P24180 |
PhylomeDBi | P24180 |
Family and domain databases
InterProi | View protein in InterPro IPR043602, CusB_dom_1 IPR032317, HlyD_D23 IPR006143, RND_pump_MFP |
Pfami | View protein in Pfam PF00529, HlyD, 1 hit PF16576, HlyD_D23, 1 hit |
TIGRFAMsi | TIGR01730, RND_mfp, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P24180-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKHARFFLL PSFILISAAL IAGCNDKGEE KAHVGEPQVT VHIVKTAPLE
60 70 80 90 100
VKTELPGRTN AYRIAEVRPQ VSGIVLNRNF TEGSDVQAGQ SLYQIDPATY
110 120 130 140 150
QANYDSAKGE LAKSEAAAAI AHLTVKRYVP LVGTKYISQQ EYDQAIADAR
160 170 180 190 200
QADAAVIAAK ATVESARINL AYTKVTAPIS GRIGKSTVTE GALVTNGQTT
210 220 230 240 250
ELATVQQLDP IYVDVTQSSN DFMRLKQSVE QGNLHKENAT SNVELVMENG
260 270 280 290 300
QTYPLKGTLQ FSDVTVDEST GSITLRAVFP NPQHTLLPGM FVRARIDEGV
310 320 330 340 350
QPDAILIPQQ GVSRTPRGDA TVLIVNDKSQ VEARPVVASQ AIGDKWLISE
360 370 380
GLKSGDQVIV SGLQKARPGE QVKATTDTPA DTASK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 191 – 212 | GALVT…LDPIY → ALLSLMGKRLNWRLSSSSIL ST (PubMed:1720861).CuratedAdd BLAST | 22 | |
Sequence conflicti | 313 – 314 | SR → T (PubMed:1720861).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M96848 Unassigned DNA Translation: AAA02931.1 X57948 Genomic DNA Translation: CAA41016.1 U18997 Genomic DNA Translation: AAA58069.1 U00096 Genomic DNA Translation: AAC76297.1 AP009048 Genomic DNA Translation: BAE77306.1 |
PIRi | C65119 |
RefSeqi | NP_417731.1, NC_000913.3 WP_000160334.1, NZ_STEB01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC76297; AAC76297; b3265 BAE77306; BAE77306; BAE77306 |
GeneIDi | 57728466 947706 |
KEGGi | ecj:JW3233 eco:b3265 |
PATRICi | fig|1411691.4.peg.3463 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M96848 Unassigned DNA Translation: AAA02931.1 X57948 Genomic DNA Translation: CAA41016.1 U18997 Genomic DNA Translation: AAA58069.1 U00096 Genomic DNA Translation: AAC76297.1 AP009048 Genomic DNA Translation: BAE77306.1 |
PIRi | C65119 |
RefSeqi | NP_417731.1, NC_000913.3 WP_000160334.1, NZ_STEB01000012.1 |
3D structure databases
SMRi | P24180 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261953, 367 interactors |
ComplexPortali | CPX-4265, AcrEF-TolC multidrug efflux transport complex |
STRINGi | 511145.b3265 |
Proteomic databases
PaxDbi | P24180 |
PRIDEi | P24180 |
Genome annotation databases
EnsemblBacteriai | AAC76297; AAC76297; b3265 BAE77306; BAE77306; BAE77306 |
GeneIDi | 57728466 947706 |
KEGGi | ecj:JW3233 eco:b3265 |
PATRICi | fig|1411691.4.peg.3463 |
Organism-specific databases
EchoBASEi | EB0262 |
Phylogenomic databases
eggNOGi | COG0845, Bacteria |
HOGENOMi | CLU_018816_2_1_6 |
InParanoidi | P24180 |
PhylomeDBi | P24180 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10266-MONOMER MetaCyc:EG10266-MONOMER |
Miscellaneous databases
PROi | PR:P24180 |
Family and domain databases
InterProi | View protein in InterPro IPR043602, CusB_dom_1 IPR032317, HlyD_D23 IPR006143, RND_pump_MFP |
Pfami | View protein in Pfam PF00529, HlyD, 1 hit PF16576, HlyD_D23, 1 hit |
TIGRFAMsi | TIGR01730, RND_mfp, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACRE_ECOLI | |
Accessioni | P24180Primary (citable) accession number: P24180 Secondary accession number(s): Q2M8V0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | July 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 148 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families