UniProtKB - P24175 (MANB_ECOLI)
Protein
Phosphomannomutase
Gene
manB
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the biosynthesis of the capsular polysaccharide colanic acid.
Catalytic activityi
- EC:5.4.2.8
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
: GDP-alpha-D-mannose biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- no protein annotated in this organism
- Phosphomannomutase (manB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 98 | Phosphoserine intermediateBy similarity | 1 | |
Metal bindingi | 98 | Magnesium; via phosphate groupBy similarity | 1 | |
Metal bindingi | 245 | MagnesiumBy similarity | 1 | |
Metal bindingi | 247 | MagnesiumBy similarity | 1 | |
Metal bindingi | 249 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- magnesium ion binding Source: InterPro
- phosphomannomutase activity Source: UniProtKB-EC
GO - Biological processi
- GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
- lipopolysaccharide biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Isomerase |
Biological process | Capsule biogenesis/degradation, Lipopolysaccharide biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:PHOSMANMUT-MONOMER MetaCyc:PHOSMANMUT-MONOMER |
UniPathwayi | UPA00126;UER00424 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:manB Synonyms:cpsG, rfbL Ordered Locus Names:b2048, JW2033 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000147818 | 1 – 456 | PhosphomannomutaseAdd BLAST | 456 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P24175 |
PRIDEi | P24175 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263309, 280 interactors |
IntActi | P24175, 9 interactors |
STRINGi | 511145.b2048 |
Family & Domainsi
Sequence similaritiesi
Belongs to the phosphohexose mutase family.Curated
Phylogenomic databases
eggNOGi | COG1109, Bacteria |
InParanoidi | P24175 |
PhylomeDBi | P24175 |
Family and domain databases
InterProi | View protein in InterPro IPR005844, A-D-PHexomutase_a/b/a-I IPR016055, A-D-PHexomutase_a/b/a-I/II/III IPR005845, A-D-PHexomutase_a/b/a-II IPR005846, A-D-PHexomutase_a/b/a-III IPR005843, A-D-PHexomutase_C IPR036900, A-D-PHexomutase_C_sf IPR016066, A-D-PHexomutase_CS IPR005841, Alpha-D-phosphohexomutase_SF |
Pfami | View protein in Pfam PF02878, PGM_PMM_I, 1 hit PF02879, PGM_PMM_II, 1 hit PF02880, PGM_PMM_III, 1 hit PF00408, PGM_PMM_IV, 1 hit |
PRINTSi | PR00509, PGMPMM |
SUPFAMi | SSF53738, SSF53738, 3 hits SSF55957, SSF55957, 1 hit |
PROSITEi | View protein in PROSITE PS00710, PGM_PMM, 1 hit |
i Sequence
Sequence statusi: Complete.
P24175-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKLTCFKAY DIRGKLGEEL NEDIAWRIGR AYGEFLKPKT IVLGGDVRLT
60 70 80 90 100
SETLKLALAK GLQDAGVDVL DIGMSGTEEI YFATFHLGVD GGIEVTASHN
110 120 130 140 150
PMDYNGMKLV REGARPISGD TGLRDVQRLA EANDFPPVDE TKRGRYQQIN
160 170 180 190 200
LRDAYVDHLF GYINVKNLTP LKLVINSGNG AAGPVVDAIE ARFKALGAPV
210 220 230 240 250
ELIKVHNTPD GNFPNGIPNP LLPECRDDTR NAVIKHGADM GIAFDGDFDR
260 270 280 290 300
CFLFDEKGQF IEGYYIVGLL AEAFLEKNPG AKIIHDPRLS WNTVDVVTAA
310 320 330 340 350
GGTPVMSKTG HAFIKERMRK EDAIYGGEMS AHHYFRDFAY CDSGMIPWLL
360 370 380 390 400
VAELVCLKDK TLGELVRDRM AAFPASGEIN SKLAQPVEAI NRVEQHFSRE
410 420 430 440 450
ALAVDRTDGI SMTFADWRFN LRTSNTEPVV RLNVESRGDV PLMEARTRTL
LTLLNE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M77127 Genomic DNA Translation: AAA02894.1 U38473 Genomic DNA Translation: AAC77847.1 U00096 Genomic DNA Translation: AAC75109.1 AP009048 Genomic DNA Translation: BAA15901.1 |
PIRi | B55239 |
RefSeqi | NP_416552.1, NC_000913.3 WP_001350528.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75109; AAC75109; b2048 BAA15901; BAA15901; BAA15901 |
GeneIDi | 946574 |
KEGGi | ecj:JW2033 eco:b2048 |
PATRICi | fig|511145.12.peg.2125 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M77127 Genomic DNA Translation: AAA02894.1 U38473 Genomic DNA Translation: AAC77847.1 U00096 Genomic DNA Translation: AAC75109.1 AP009048 Genomic DNA Translation: BAA15901.1 |
PIRi | B55239 |
RefSeqi | NP_416552.1, NC_000913.3 WP_001350528.1, NZ_LN832404.1 |
3D structure databases
SMRi | P24175 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263309, 280 interactors |
IntActi | P24175, 9 interactors |
STRINGi | 511145.b2048 |
Proteomic databases
PaxDbi | P24175 |
PRIDEi | P24175 |
Genome annotation databases
EnsemblBacteriai | AAC75109; AAC75109; b2048 BAA15901; BAA15901; BAA15901 |
GeneIDi | 946574 |
KEGGi | ecj:JW2033 eco:b2048 |
PATRICi | fig|511145.12.peg.2125 |
Organism-specific databases
EchoBASEi | EB0160 |
Phylogenomic databases
eggNOGi | COG1109, Bacteria |
InParanoidi | P24175 |
PhylomeDBi | P24175 |
Enzyme and pathway databases
UniPathwayi | UPA00126;UER00424 |
BioCyci | EcoCyc:PHOSMANMUT-MONOMER MetaCyc:PHOSMANMUT-MONOMER |
Miscellaneous databases
PROi | PR:P24175 |
Family and domain databases
InterProi | View protein in InterPro IPR005844, A-D-PHexomutase_a/b/a-I IPR016055, A-D-PHexomutase_a/b/a-I/II/III IPR005845, A-D-PHexomutase_a/b/a-II IPR005846, A-D-PHexomutase_a/b/a-III IPR005843, A-D-PHexomutase_C IPR036900, A-D-PHexomutase_C_sf IPR016066, A-D-PHexomutase_CS IPR005841, Alpha-D-phosphohexomutase_SF |
Pfami | View protein in Pfam PF02878, PGM_PMM_I, 1 hit PF02879, PGM_PMM_II, 1 hit PF02880, PGM_PMM_III, 1 hit PF00408, PGM_PMM_IV, 1 hit |
PRINTSi | PR00509, PGMPMM |
SUPFAMi | SSF53738, SSF53738, 3 hits SSF55957, SSF55957, 1 hit |
PROSITEi | View protein in PROSITE PS00710, PGM_PMM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MANB_ECOLI | |
Accessioni | P24175Primary (citable) accession number: P24175 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | March 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families