UniProtKB - P24174 (MANC_ECOLI)
Protein
Mannose-1-phosphate guanylyltransferase
Gene
manC
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the biosynthesis of the capsular polysaccharide colanic acid.
Catalytic activityi
- EC:2.7.7.13
: GDP-alpha-D-mannose biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route).Proteins known to be involved in this subpathway in this organism are:
- Mannose-1-phosphate guanylyltransferase (cpsB), Mannose-1-phosphate guanylyltransferase (manC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route), the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.
GO - Molecular functioni
- GTP binding Source: UniProtKB-KW
- mannose-1-phosphate guanylyltransferase activity Source: EcoCyc
GO - Biological processi
- colanic acid biosynthetic process Source: EcoCyc
- GDP-mannose biosynthetic process Source: GO_Central
- hyperosmotic response Source: EcoCyc
- lipopolysaccharide biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Capsule biogenesis/degradation, Lipopolysaccharide biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:MANNPGUANYLTRANGDP-MONOMER MetaCyc:MANNPGUANYLTRANGDP-MONOMER |
UniPathwayi | UPA00126;UER00930 |
Names & Taxonomyi
Protein namesi | Recommended name: Mannose-1-phosphate guanylyltransferase (EC:2.7.7.13)Alternative name(s): GDP-mannose pyrophosphorylase Short name: GMP Short name: GMPP |
Gene namesi | Name:manC Synonyms:cpsB, rfbM Ordered Locus Names:b2049, JW2034 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194253 | 1 – 478 | Mannose-1-phosphate guanylyltransferaseAdd BLAST | 478 |
Proteomic databases
PaxDbi | P24174 |
PRIDEi | P24174 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263312, 190 interactors |
IntActi | P24174, 5 interactors |
STRINGi | 511145.b2049 |
Family & Domainsi
Sequence similaritiesi
Belongs to the mannose-6-phosphate isomerase type 2 family.Curated
Phylogenomic databases
eggNOGi | COG0662, Bacteria COG0836, Bacteria |
HOGENOMi | CLU_035527_1_0_6 |
InParanoidi | P24174 |
PhylomeDBi | P24174 |
Family and domain databases
Gene3Di | 2.60.120.10, 1 hit 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR006375, Man1P_GuaTrfase/Man6P_Isoase IPR001538, Man6P_isomerase-2_C IPR005835, NTP_transferase_dom IPR029044, Nucleotide-diphossugar_trans IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
Pfami | View protein in Pfam PF01050, MannoseP_isomer, 1 hit PF00483, NTP_transferase, 1 hit |
SUPFAMi | SSF51182, SSF51182, 1 hit SSF53448, SSF53448, 1 hit |
TIGRFAMsi | TIGR01479, GMP_PMI, 1 hit |
i Sequence
Sequence statusi: Complete.
P24174-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQSKLYPVV MAGGSGSRLW PLSRVLYPKQ FLCLKGDLTM LQTTICRLNG
60 70 80 90 100
VECESPVVIC NEQHRFIVAE QLRQLNKLTE NIILEPAGRN TAPAIALAAL
110 120 130 140 150
AAKRHSPESD PLMLVLAADH VIADEDAFRA AVRNAMPYAE AGKLVTFGIV
160 170 180 190 200
PDLPETGYGY IRRGEVSAGE QDMVAFEVAQ FVEKPNLETA QAYVASGEYY
210 220 230 240 250
WNSGMFLFRA GRYLEELKKY RPDILDACEK AMSAVDPDLN FIRVDEEAFL
260 270 280 290 300
ACPEESVDYA VMERTADAVV VPMDAGWSDV GSWSSLWEIS AHTAEGNVCH
310 320 330 340 350
GDVINHKTEN SYVYAESGLV TTVGVKDLVV VQTKDAVLIA DRNAVQDVKK
360 370 380 390 400
VVEQIKADGR HEHRVHREVY RPWGKYDSID AGDRYQVKRI TVKPGEGLSV
410 420 430 440 450
QMHHHRAEHW VVVAGTAKVT IDGDIKLLGE NESIYIPLGA THCLENPGKI
460 470
PLDLIEVRSG SYLEEDDVVR FADRYGRV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 325 | V → L (PubMed:7815923).Curated | 1 | |
Sequence conflicti | 325 | V → L (PubMed:8759852).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U38473 Genomic DNA Translation: AAC77846.1 U00096 Genomic DNA Translation: AAC75110.1 AP009048 Genomic DNA Translation: BAA15905.1 M77127 Genomic DNA Translation: AAA02893.1 |
PIRi | H64970 |
RefSeqi | NP_416553.1, NC_000913.3 WP_000079274.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75110; AAC75110; b2049 BAA15905; BAA15905; BAA15905 |
GeneIDi | 946580 |
KEGGi | ecj:JW2034 eco:b2049 |
PATRICi | fig|1411691.4.peg.202 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U38473 Genomic DNA Translation: AAC77846.1 U00096 Genomic DNA Translation: AAC75110.1 AP009048 Genomic DNA Translation: BAA15905.1 M77127 Genomic DNA Translation: AAA02893.1 |
PIRi | H64970 |
RefSeqi | NP_416553.1, NC_000913.3 WP_000079274.1, NZ_LN832404.1 |
3D structure databases
SMRi | P24174 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263312, 190 interactors |
IntActi | P24174, 5 interactors |
STRINGi | 511145.b2049 |
Proteomic databases
PaxDbi | P24174 |
PRIDEi | P24174 |
Genome annotation databases
EnsemblBacteriai | AAC75110; AAC75110; b2049 BAA15905; BAA15905; BAA15905 |
GeneIDi | 946580 |
KEGGi | ecj:JW2034 eco:b2049 |
PATRICi | fig|1411691.4.peg.202 |
Organism-specific databases
EchoBASEi | EB0159 |
Phylogenomic databases
eggNOGi | COG0662, Bacteria COG0836, Bacteria |
HOGENOMi | CLU_035527_1_0_6 |
InParanoidi | P24174 |
PhylomeDBi | P24174 |
Enzyme and pathway databases
UniPathwayi | UPA00126;UER00930 |
BioCyci | EcoCyc:MANNPGUANYLTRANGDP-MONOMER MetaCyc:MANNPGUANYLTRANGDP-MONOMER |
Miscellaneous databases
PROi | PR:P24174 |
Family and domain databases
Gene3Di | 2.60.120.10, 1 hit 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR006375, Man1P_GuaTrfase/Man6P_Isoase IPR001538, Man6P_isomerase-2_C IPR005835, NTP_transferase_dom IPR029044, Nucleotide-diphossugar_trans IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
Pfami | View protein in Pfam PF01050, MannoseP_isomer, 1 hit PF00483, NTP_transferase, 1 hit |
SUPFAMi | SSF51182, SSF51182, 1 hit SSF53448, SSF53448, 1 hit |
TIGRFAMsi | TIGR01479, GMP_PMI, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MANC_ECOLI | |
Accessioni | P24174Primary (citable) accession number: P24174 Secondary accession number(s): P78084 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | July 19, 2003 | |
Last modified: | December 2, 2020 | |
This is version 160 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families