manC

Functionⁱ

Involved in the biosynthesis of the capsular polysaccharide colanic acid.

Catalytic activityⁱ

GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.

Pathwayⁱ: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route).
Proteins known to be involved in this subpathway in this organism are:

Mannose-1-phosphate guanylyltransferase (manC)

This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route), the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

GO - Molecular functionⁱ

GTP binding Source: UniProtKB-KW
mannose-1-phosphate guanylyltransferase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 4908790
transferase activity, transferring glycosyl groups Source: GO_Central

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

colanic acid biosynthetic process
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 6090411
GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
hyperosmotic response
Source: EcoCyc
Inferred from expression patternⁱ
- 8576059
lipopolysaccharide biosynthetic process Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Nucleotidyltransferase, Transferase
Biological process	Capsule biogenesis/degradation, Lipopolysaccharide biosynthesis
Ligand	GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:MANNPGUANYLTRANGDP-MONOMER MetaCyc:MANNPGUANYLTRANGDP-MONOMER
UniPathwayⁱ	UPA00126;UER00930

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Mannose-1-phosphate guanylyltransferase (EC:2.7.7.13) Alternative name(s): GDP-mannose pyrophosphorylase Short name: GMP Short name: GMPP
Gene namesⁱ	Name:manC Synonyms:cpsB, rfbM Ordered Locus Names:b2049, JW2034
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10161 manC

EcoGeneⁱ

EG10161 manC

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000194253	1 – 478	Mannose-1-phosphate guanylyltransferaseAdd BLAST		478

Proteomic databases

PaxDbⁱ	P24174
PRIDEⁱ	P24174

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4263312, 190 interactors
IntActⁱ	P24174, 5 interactors
STRINGⁱ	316385.ECDH10B_2199

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P24174
SMRⁱ	P24174
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the mannose-6-phosphate isomerase type 2 family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105CJT Bacteria COG0662 LUCA COG0836 LUCA
HOGENOMⁱ	HOG000153549
InParanoidⁱ	P24174
KEGG Orthology (KO) More...KOⁱ	K00971
OMAⁱ	WSDLGNF
PhylomeDBⁱ	P24174

Family and domain databases

Gene3Dⁱ	2.60.120.10, 1 hit 3.90.550.10, 1 hit
InterProⁱ	View protein in InterPro IPR006375 Man1P_GuaTrfase/Man6P_Isoase IPR001538 Man6P_isomerase-2_C IPR005835 NTP_transferase_dom IPR029044 Nucleotide-diphossugar_trans IPR014710 RmlC-like_jellyroll IPR011051 RmlC_Cupin_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01050 MannoseP_isomer, 1 hit PF00483 NTP_transferase, 1 hit
SUPFAMⁱ	SSF51182 SSF51182, 1 hit SSF53448 SSF53448, 1 hit
TIGRFAMsⁱ	TIGR01479 GMP_PMI, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P24174-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAQSKLYPVV MAGGSGSRLW PLSRVLYPKQ FLCLKGDLTM LQTTICRLNG 
        60         70         80         90        100
VECESPVVIC NEQHRFIVAE QLRQLNKLTE NIILEPAGRN TAPAIALAAL 
       110        120        130        140        150
AAKRHSPESD PLMLVLAADH VIADEDAFRA AVRNAMPYAE AGKLVTFGIV 
       160        170        180        190        200
PDLPETGYGY IRRGEVSAGE QDMVAFEVAQ FVEKPNLETA QAYVASGEYY 
       210        220        230        240        250
WNSGMFLFRA GRYLEELKKY RPDILDACEK AMSAVDPDLN FIRVDEEAFL 
       260        270        280        290        300
ACPEESVDYA VMERTADAVV VPMDAGWSDV GSWSSLWEIS AHTAEGNVCH 
       310        320        330        340        350
GDVINHKTEN SYVYAESGLV TTVGVKDLVV VQTKDAVLIA DRNAVQDVKK 
       360        370        380        390        400
VVEQIKADGR HEHRVHREVY RPWGKYDSID AGDRYQVKRI TVKPGEGLSV 
       410        420        430        440        450
QMHHHRAEHW VVVAGTAKVT IDGDIKLLGE NESIYIPLGA THCLENPGKI 
       460        470 
PLDLIEVRSG SYLEEDDVVR FADRYGRV

Length:478

Mass (Da):53,016

Last modified:July 19, 2003 - v3

Checksum:ⁱ675C0EEA050B4186

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	325	V → L (PubMed:7815923).Curated		1
Sequence conflictⁱ	325	V → L (PubMed:8759852).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U38473 Genomic DNA Translation: AAC77846.1 U00096 Genomic DNA Translation: AAC75110.1 AP009048 Genomic DNA Translation: BAA15905.1 M77127 Genomic DNA Translation: AAA02893.1
PIRⁱ	H64970
NCBI Reference Sequences More...RefSeqⁱ	NP_416553.1, NC_000913.3 WP_000079274.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC75110; AAC75110; b2049 BAA15905; BAA15905; BAA15905
GeneIDⁱ	946580
KEGGⁱ	ecj:JW2034 eco:b2049
PATRICⁱ	fig\|1411691.4.peg.202

Protein	Similar proteins		Species	Score	Length	Source
P24174	Mannose-1-phosphate guanylyltransferase		ECOLX		478	UniRef100_P24174
Mannose-1-phosphate guanylyltransferase protein (Fragment)		human gut metagenome		91
Mannose-1-phosphate guanylyltransferase (GDP)/mannose-6-phosphate isomerase		uncultured bacterium		93
Mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (Fragment)		ACINO		76

Protein	Similar proteins		Species	Score	Length	Source
P24174	Mannose-1-phosphate guanylyltransferase ManC		SALTY		480	UniRef90_P24174
Mannose-1-phosphate guanylyltransferase 1		ECO57		478
Mannose-1-phosphate guanylyltransferase		ECOLX		478
Mannose-1-phosphate guanylyltransferase protein (Fragment)		human gut metagenome		91
Mannose-1-phosphate guanylyltransferase (GDP)/mannose-6-phosphate isomerase		uncultured bacterium		93
+1410

Protein	Similar proteins		Species	Score	Length	Source
P24174	Mannose-1-phosphate guanylyltransferase ManC		SALTY		480	UniRef50_P24174
Mannose-1-phosphate guanylyltransferase 1		ECO57		478
Mannose-1-phosphate guanylyltransferase		ECOLX		478
Mannose-1-phosphate guanylyltransferase protein (Fragment)		human gut metagenome		91
Mannose-1-phosphate guanylyltransferase (GDP)/mannose-6-phosphate isomerase		uncultured bacterium		93
+1420

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U38473 Genomic DNA Translation: AAC77846.1 U00096 Genomic DNA Translation: AAC75110.1 AP009048 Genomic DNA Translation: BAA15905.1 M77127 Genomic DNA Translation: AAA02893.1
PIRⁱ	H64970
RefSeqⁱ	NP_416553.1, NC_000913.3 WP_000079274.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P24174
SMRⁱ	P24174
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4263312, 190 interactors
IntActⁱ	P24174, 5 interactors
STRINGⁱ	316385.ECDH10B_2199

Proteomic databases

PaxDbⁱ	P24174
PRIDEⁱ	P24174

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC75110; AAC75110; b2049 BAA15905; BAA15905; BAA15905
GeneIDⁱ	946580
KEGGⁱ	ecj:JW2034 eco:b2049
PATRICⁱ	fig\|1411691.4.peg.202

Organism-specific databases

EchoBASEⁱ	EB0159
EcoGeneⁱ	EG10161 manC

Phylogenomic databases

eggNOGⁱ	ENOG4105CJT Bacteria COG0662 LUCA COG0836 LUCA
HOGENOMⁱ	HOG000153549
InParanoidⁱ	P24174
KOⁱ	K00971
OMAⁱ	WSDLGNF
PhylomeDBⁱ	P24174

Enzyme and pathway databases

UniPathwayⁱ	UPA00126;UER00930
BioCycⁱ	EcoCyc:MANNPGUANYLTRANGDP-MONOMER MetaCyc:MANNPGUANYLTRANGDP-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P24174

PROⁱ

PR:P24174

Family and domain databases

Gene3Dⁱ	2.60.120.10, 1 hit 3.90.550.10, 1 hit
InterProⁱ	View protein in InterPro IPR006375 Man1P_GuaTrfase/Man6P_Isoase IPR001538 Man6P_isomerase-2_C IPR005835 NTP_transferase_dom IPR029044 Nucleotide-diphossugar_trans IPR014710 RmlC-like_jellyroll IPR011051 RmlC_Cupin_sf
Pfamⁱ	View protein in Pfam PF01050 MannoseP_isomer, 1 hit PF00483 NTP_transferase, 1 hit
SUPFAMⁱ	SSF51182 SSF51182, 1 hit SSF53448 SSF53448, 1 hit
TIGRFAMsⁱ	TIGR01479 GMP_PMI, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MANC_ECOLI
Accessionⁱ	P24174Primary (citable) accession number: P24174 Secondary accession number(s): P78084
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
	Last sequence update:	July 19, 2003
	Last modified:	March 28, 2018
	This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

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UniRef

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Annotation systems

Supporting data

UniProtKB - P24174 (MANC_ECOLI)

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Mannose-1-phosphate guanylyltransferase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: GDP-alpha-D-mannose biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: GDP-alpha-D-mannose biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ