UniProtKB - P24171 (DCP_ECOLI)
Protein
Dipeptidyl carboxypeptidase
Gene
dcp
Organism
Escherichia coli (strain K12)
Status
Functioni
Removes dipeptides from the C-termini of N-blocked tripeptides, tetrapeptides and larger peptides.1 Publication
Catalytic activityi
- Hydrolysis of unblocked, C-terminal dipeptides from oligopeptides, with broad specificity. Does not hydrolyze bonds in which P1' is Pro, or both P1 and P1' are Gly. EC:3.4.15.5
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion.By similarity
Activity regulationi
Stimulated by Mn2+, Mg2+, Co2+ and Ca2+, inhibited by Cu2+, Ni2+, Zn2+, chymostatin and 1,10-phenanthroline.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 470 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 471 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 474 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 477 | Zinc; catalyticPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- carboxypeptidase activity Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
- metalloendopeptidase activity Source: InterPro
- metallopeptidase activity Source: GO_Central
GO - Biological processi
- proteolysis Source: EcoCyc
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10212-MONOMER MetaCyc:EG10212-MONOMER |
BRENDAi | 3.4.15.5, 2026 |
Protein family/group databases
MEROPSi | M03.005 |
Names & Taxonomyi
Protein namesi | Recommended name: Dipeptidyl carboxypeptidase1 Publication (EC:3.4.15.5)Alternative name(s): Peptidyl-dipeptidase Dcp |
Gene namesi | Name:dcp1 Publication Ordered Locus Names:b1538, JW1531 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm 1 Publication
GO - Cellular componenti
- cytoplasm Source: EcoCyc
- cytosol Source: EcoCyc
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Unable to grow on N-benzoyl-Gly-His-Leu as a nitrogen source.1 Publication
Chemistry databases
ChEMBLi | CHEMBL3259514 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000078157 | 2 – 681 | Dipeptidyl carboxypeptidaseAdd BLAST | 680 |
Proteomic databases
jPOSTi | P24171 |
PaxDbi | P24171 |
PRIDEi | P24171 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261739, 52 interactors |
IntActi | P24171, 4 interactors |
STRINGi | 511145.b1538 |
Chemistry databases
BindingDBi | P24171 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P24171 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24171 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase M3 family.Curated
Phylogenomic databases
eggNOGi | COG0339, Bacteria |
HOGENOMi | CLU_001805_4_0_6 |
InParanoidi | P24171 |
PhylomeDBi | P24171 |
Family and domain databases
Gene3Di | 1.10.1370.10, 1 hit |
InterProi | View protein in InterPro IPR024077, Neurolysin/TOP_dom2 IPR001567, Pept_M3A_M3B |
Pfami | View protein in Pfam PF01432, Peptidase_M3, 1 hit |
PROSITEi | View protein in PROSITE PS00142, ZINC_PROTEASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P24171-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTMNPFLVQ STLPYLAPHF DQIANHHYRP AFDEGMQQKR AEIAAIALNP
60 70 80 90 100
QMPDFNNTIL ALEQSGELLT RVTSVFFAMT AAHTNDELQR LDEQFSAELA
110 120 130 140 150
ELANDIYLNG ELFARVDAVW QRRESLGLDS ESIRLVEVIH QRFVLAGAKL
160 170 180 190 200
AQADKAKLKV LNTEAATLTS QFNQRLLAAN KSGGLVVNDI AQLAGMSEQE
210 220 230 240 250
IALAAEAARE KGLDNKWLIP LLNTTQQPAL AEMRDRATRE KLFIAGWTRA
260 270 280 290 300
EKNDANDTRA IIQRLVEIRA QQATLLGFPH YAAWKIADQM AKTPEAALNF
310 320 330 340 350
MREIVPAARQ RASDELASIQ AVIDKQQGGF SAQPWDWAFY AEQVRREKFD
360 370 380 390 400
LDEAQLKPYF ELNTVLNEGV FWTANQLFGI KFVERFDIPV YHPDVRVWEI
410 420 430 440 450
FDHNGVGLAL FYGDFFARDS KSGGAWMGNF VEQSTLNKTH PVIYNVCNYQ
460 470 480 490 500
KPAAGEPALL LWDDVITLFH EFGHTLHGLF ARQRYATLSG TNTPRDFVEF
510 520 530 540 550
PSQINEHWAT HPQVFARYAR HYQSGAAMPD ELQQKMRNAS LFNKGYEMSE
560 570 580 590 600
LLSAALLDMR WHCLEENEAM QDVDDFELRA LVAENMDLPA IPPRYRSSYF
610 620 630 640 650
AHIFGGGYAA GYYAYLWTQM LADDGYQWFV EQGGLTRENG LRFREAILSR
660 670 680
GNSEDLERLY RQWRGKAPKI MPMLQHRGLN I
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 139 – 140 | IH → LL in CAA41014 (PubMed:8226676).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57947 Genomic DNA Translation: CAA41014.1 U00096 Genomic DNA Translation: AAC74611.1 AP009048 Genomic DNA Translation: BAA15228.1 |
PIRi | E64908 |
RefSeqi | NP_416056.1, NC_000913.3 WP_000210373.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74611; AAC74611; b1538 BAA15228; BAA15228; BAA15228 |
GeneIDi | 946084 |
KEGGi | ecj:JW1531 eco:b1538 |
PATRICi | fig|1411691.4.peg.727 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57947 Genomic DNA Translation: CAA41014.1 U00096 Genomic DNA Translation: AAC74611.1 AP009048 Genomic DNA Translation: BAA15228.1 |
PIRi | E64908 |
RefSeqi | NP_416056.1, NC_000913.3 WP_000210373.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Y79 | X-ray | 2.00 | 1 | 2-681 | [»] | |
SMRi | P24171 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261739, 52 interactors |
IntActi | P24171, 4 interactors |
STRINGi | 511145.b1538 |
Chemistry databases
BindingDBi | P24171 |
ChEMBLi | CHEMBL3259514 |
Protein family/group databases
MEROPSi | M03.005 |
Proteomic databases
jPOSTi | P24171 |
PaxDbi | P24171 |
PRIDEi | P24171 |
Genome annotation databases
EnsemblBacteriai | AAC74611; AAC74611; b1538 BAA15228; BAA15228; BAA15228 |
GeneIDi | 946084 |
KEGGi | ecj:JW1531 eco:b1538 |
PATRICi | fig|1411691.4.peg.727 |
Organism-specific databases
EchoBASEi | EB0208 |
Phylogenomic databases
eggNOGi | COG0339, Bacteria |
HOGENOMi | CLU_001805_4_0_6 |
InParanoidi | P24171 |
PhylomeDBi | P24171 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10212-MONOMER MetaCyc:EG10212-MONOMER |
BRENDAi | 3.4.15.5, 2026 |
Miscellaneous databases
EvolutionaryTracei | P24171 |
PROi | PR:P24171 |
Family and domain databases
Gene3Di | 1.10.1370.10, 1 hit |
InterProi | View protein in InterPro IPR024077, Neurolysin/TOP_dom2 IPR001567, Pept_M3A_M3B |
Pfami | View protein in Pfam PF01432, Peptidase_M3, 1 hit |
PROSITEi | View protein in PROSITE PS00142, ZINC_PROTEASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCP_ECOLI | |
Accessioni | P24171Primary (citable) accession number: P24171 Secondary accession number(s): P78305 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 172 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries