UniProtKB - P24158 (PRTN3_HUMAN)
Myeloblastin
PRTN3
Functioni
Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) (PubMed:3198760, PubMed:2033050, PubMed:28240246).
By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed:23202369).
May play a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed:22266279).
5 PublicationsCatalytic activityi
- Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-.6 Publications EC:3.4.21.76
Activity regulationi
pH dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 71 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 118 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 203 | Charge relay systemPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- enzyme binding Source: UniProtKB
- serine-type endopeptidase activity Source: UniProtKB
- serine-type peptidase activity Source: ProtInc
- signaling receptor binding Source: UniProtKB
GO - Biological processi
- antimicrobial humoral response Source: Reactome
- cell-cell junction maintenance Source: UniProtKB
- collagen catabolic process Source: UniProtKB-KW
- mature conventional dendritic cell differentiation Source: UniProtKB
- membrane protein ectodomain proteolysis Source: UniProtKB
- negative regulation of phagocytosis Source: UniProtKB
- neutrophil extravasation Source: UniProtKB
- phagocytosis Source: GO_Central
- positive regulation of cell population proliferation Source: ProtInc
- positive regulation of GTPase activity Source: UniProtKB
- proteolysis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Collagen degradation |
Enzyme and pathway databases
BRENDAi | 3.4.21.76, 2681 |
PathwayCommonsi | P24158 |
Reactomei | R-HSA-140875, Common Pathway of Fibrin Clot Formation R-HSA-449836, Other interleukin signaling R-HSA-6798695, Neutrophil degranulation R-HSA-6803157, Antimicrobial peptides |
SABIO-RKi | P24158 |
SignaLinki | P24158 |
SIGNORi | P24158 |
Protein family/group databases
MEROPSi | S01.134 |
Names & Taxonomyi
Protein namesi | Recommended name: Myeloblastin (EC:3.4.21.766 Publications)Alternative name(s): AGP7 C-ANCA antigen Leukocyte proteinase 3 Short name: PR-3 Short name: PR3 Neutrophil proteinase 4 Short name: NP-4 P29 Wegener autoantigen |
Gene namesi | Name:PRTN3 Synonyms:MBN |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9495, PRTN3 |
MIMi | 177020, gene |
neXtProti | NX_P24158 |
VEuPathDBi | HostDB:ENSG00000196415 |
Subcellular locationi
Plasma membrane
- Cell membrane 4 Publications; Peripheral membrane protein 4 Publications; Extracellular side 4 Publications
Extracellular region or secreted
- Secreted 2 Publications
Other locations
- Cytoplasmic granule 1 Publication4 Publications
- Membrane raft 1 Publication; Peripheral membrane protein 1 Publication; Extracellular side 1 Publication
Note: Localizes predominantly to azurophil granules (primary secretory granules) in neutrophils (PubMed:2033050, PubMed:3198760, PubMed:7897245, PubMed:18462208). Secreted upon neutrophil stimulation by TNF-alpha, lipopolysaccharide (LPS), fMLP and CXCL8/IL8 or during neutrophil transmigration (PubMed:22266279, PubMed:28240246). Following secretion tethered to the cell membrane by CD177 (PubMed:18462208, PubMed:22266279).6 Publications
Cytosol
- cytosol Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: Reactome
- extracellular space Source: UniProtKB
Lysosome
- azurophil granule lumen Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
- plasma membrane raft Source: UniProtKB
Other locations
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
Cell membrane, Membrane, SecretedPathology & Biotechi
Involvement in diseasei
Organism-specific databases
DisGeNETi | 5657 |
MalaCardsi | PRTN3 |
OpenTargetsi | ENSG00000196415 |
Orphaneti | 900, Granulomatosis with polyangiitis |
PharmGKBi | PA33842 |
Miscellaneous databases
Pharosi | P24158, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3900 |
DrugBanki | DB05161, Elafin |
DrugCentrali | P24158 |
GuidetoPHARMACOLOGYi | 2401 |
Genetic variation databases
BioMutai | PRTN3 |
DMDMi | 6174926 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Add BLAST | 25 | |
PropeptideiPRO_0000027707 | 26 – 27 | 2 | ||
ChainiPRO_0000027708 | 28 – 248 | Myeloblastin4 PublicationsAdd BLAST | 221 | |
PropeptideiPRO_0000027709 | 249 – 256 | 8 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 56 ↔ 72 | Combined sources1 Publication | ||
Glycosylationi | 129 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 152 ↔ 209 | Combined sources1 Publication | ||
Glycosylationi | 174 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Disulfide bondi | 182 ↔ 188 | Combined sources1 Publication | ||
Disulfide bondi | 199 ↔ 224 | Combined sources1 Publication |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
CPTACi | non-CPTAC-1142 |
EPDi | P24158 |
jPOSTi | P24158 |
MassIVEi | P24158 |
PaxDbi | P24158 |
PeptideAtlasi | P24158 |
PRIDEi | P24158 |
ProteomicsDBi | 54192 |
PTM databases
GlyConnecti | 1529, 2 N-Linked glycans (1 site) |
GlyGeni | P24158, 3 sites, 1 N-linked glycan (1 site) |
iPTMneti | P24158 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000196415, Expressed in bone marrow and 91 other tissues |
ExpressionAtlasi | P24158, baseline and differential |
Genevisiblei | P24158, HS |
Organism-specific databases
HPAi | ENSG00000196415, Tissue enriched (bone) |
Interactioni
Subunit structurei
Binary interactionsi
P24158
With | #Exp. | IntAct |
---|---|---|
IL32 - isoform 4 [P24001-4] | 3 | EBI-465028,EBI-15570379 |
GO - Molecular functioni
- enzyme binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 111638, 107 interactors |
DIPi | DIP-31107N |
IntActi | P24158, 7 interactors |
MINTi | P24158 |
STRINGi | 9606.ENSP00000234347 |
Chemistry databases
BindingDBi | P24158 |
Miscellaneous databases
RNActi | P24158, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P24158 |
SMRi | P24158 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P24158 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 248 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 221 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT01030000234528 |
HOGENOMi | CLU_006842_1_0_1 |
InParanoidi | P24158 |
OMAi | IQLNRPA |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P24158 |
TreeFami | TF335284 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAHRPPSPAL ASVLLALLLS GAARAAEIVG GHEAQPHSRP YMASLQMRGN
60 70 80 90 100
PGSHFCGGTL IHPSFVLTAA HCLRDIPQRL VNVVLGAHNV RTQEPTQQHF
110 120 130 140 150
SVAQVFLNNY DAENKLNDVL LIQLSSPANL SASVATVQLP QQDQPVPHGT
160 170 180 190 200
QCLAMGWGRV GAHDPPAQVL QELNVTVVTF FCRPHNICTF VPRRKAGICF
210 220 230 240 250
GDSGGPLICD GIIQGIDSFV IWGCATRLFP DFFTRVALYV DWIRSTLRRV
EAKGRP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketU3KPS2 | U3KPS2_HUMAN | Myeloblastin | PRTN3 | 215 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | A → R in CAA39203 (PubMed:2258701).Curated | 1 | |
Sequence conflicti | 38 | S → I AA sequence (PubMed:1688612).Curated | 1 | |
Sequence conflicti | 40 | P → PI AA sequence (PubMed:1688612).Curated | 1 | |
Sequence conflicti | 46 | Q → E AA sequence (PubMed:2404977).Curated | 1 | |
Sequence conflicti | 46 | Q → E AA sequence (PubMed:2501794).Curated | 1 | |
Sequence conflicti | 48 | R → A AA sequence (PubMed:2121162).Curated | 1 | |
Sequence conflicti | 64 | S → D AA sequence (PubMed:2033050).Curated | 1 | |
Sequence conflicti | 70 | A → P in AAA59558 (PubMed:1681549).Curated | 1 | |
Sequence conflicti | 255 | Missing in CAA39203 (PubMed:2258701).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_011691 | 119 | V → I7 PublicationsCorresponds to variant dbSNP:rs351111Ensembl. | 1 | |
Natural variantiVAR_011713 | 135 | A → T4 PublicationsCorresponds to variant dbSNP:rs1042281Ensembl. | 1 | |
Natural variantiVAR_011714 | 136 | T → S4 PublicationsCorresponds to variant dbSNP:rs1042282Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M75154 mRNA Translation: AAA59558.1 AC004799 Genomic DNA No translation available. CH471139 Genomic DNA Translation: EAW69591.1 BC096183 mRNA Translation: AAH96183.1 BC096184 mRNA Translation: AAH96184.1 BC096185 mRNA Translation: AAH96185.1 BC096186 mRNA Translation: AAH96186.1 M96628 Genomic DNA Translation: AAB59364.1 AH005293 Genomic DNA Translation: AAB59493.1 M97911 Genomic DNA No translation available. AH007523 Genomic DNA Translation: AAD21524.1 X56606 mRNA Translation: CAA39943.1 X55668 mRNA Translation: CAA39203.1 M29142 mRNA Translation: AAA36342.1 Frameshift. X56132 mRNA Translation: CAA39597.1 X56132 mRNA Translation: CAA39598.1 Different initiation. |
CCDSi | CCDS32860.1 |
PIRi | A45080, PRHU3 |
RefSeqi | NP_002768.3, NM_002777.3 |
Genome annotation databases
Ensembli | ENST00000234347.10; ENSP00000234347.3; ENSG00000196415.10 ENST00000612112.4; ENSP00000478977.1; ENSG00000277804.4 |
GeneIDi | 5657 |
KEGGi | hsa:5657 |
MANE-Selecti | ENST00000234347.10; ENSP00000234347.3; NM_002777.4; NP_002768.3 |
UCSCi | uc002lqa.2, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia Proteinase 3 entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M75154 mRNA Translation: AAA59558.1 AC004799 Genomic DNA No translation available. CH471139 Genomic DNA Translation: EAW69591.1 BC096183 mRNA Translation: AAH96183.1 BC096184 mRNA Translation: AAH96184.1 BC096185 mRNA Translation: AAH96185.1 BC096186 mRNA Translation: AAH96186.1 M96628 Genomic DNA Translation: AAB59364.1 AH005293 Genomic DNA Translation: AAB59493.1 M97911 Genomic DNA No translation available. AH007523 Genomic DNA Translation: AAD21524.1 X56606 mRNA Translation: CAA39943.1 X55668 mRNA Translation: CAA39203.1 M29142 mRNA Translation: AAA36342.1 Frameshift. X56132 mRNA Translation: CAA39597.1 X56132 mRNA Translation: CAA39598.1 Different initiation. |
CCDSi | CCDS32860.1 |
PIRi | A45080, PRHU3 |
RefSeqi | NP_002768.3, NM_002777.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FUJ | X-ray | 2.20 | A/B/C/D | 28-248 | [»] | |
AlphaFoldDBi | P24158 | |||||
SMRi | P24158 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111638, 107 interactors |
DIPi | DIP-31107N |
IntActi | P24158, 7 interactors |
MINTi | P24158 |
STRINGi | 9606.ENSP00000234347 |
Chemistry databases
BindingDBi | P24158 |
ChEMBLi | CHEMBL3900 |
DrugBanki | DB05161, Elafin |
DrugCentrali | P24158 |
GuidetoPHARMACOLOGYi | 2401 |
Protein family/group databases
MEROPSi | S01.134 |
PTM databases
GlyConnecti | 1529, 2 N-Linked glycans (1 site) |
GlyGeni | P24158, 3 sites, 1 N-linked glycan (1 site) |
iPTMneti | P24158 |
Genetic variation databases
BioMutai | PRTN3 |
DMDMi | 6174926 |
Proteomic databases
CPTACi | non-CPTAC-1142 |
EPDi | P24158 |
jPOSTi | P24158 |
MassIVEi | P24158 |
PaxDbi | P24158 |
PeptideAtlasi | P24158 |
PRIDEi | P24158 |
ProteomicsDBi | 54192 |
Protocols and materials databases
ABCDi | P24158, 4 sequenced antibodies |
Antibodypediai | 1069, 524 antibodies from 39 providers |
DNASUi | 5657 |
Genome annotation databases
Ensembli | ENST00000234347.10; ENSP00000234347.3; ENSG00000196415.10 ENST00000612112.4; ENSP00000478977.1; ENSG00000277804.4 |
GeneIDi | 5657 |
KEGGi | hsa:5657 |
MANE-Selecti | ENST00000234347.10; ENSP00000234347.3; NM_002777.4; NP_002768.3 |
UCSCi | uc002lqa.2, human |
Organism-specific databases
CTDi | 5657 |
DisGeNETi | 5657 |
GeneCardsi | PRTN3 |
HGNCi | HGNC:9495, PRTN3 |
HPAi | ENSG00000196415, Tissue enriched (bone) |
MalaCardsi | PRTN3 |
MIMi | 177020, gene |
neXtProti | NX_P24158 |
OpenTargetsi | ENSG00000196415 |
Orphaneti | 900, Granulomatosis with polyangiitis |
PharmGKBi | PA33842 |
VEuPathDBi | HostDB:ENSG00000196415 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT01030000234528 |
HOGENOMi | CLU_006842_1_0_1 |
InParanoidi | P24158 |
OMAi | IQLNRPA |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P24158 |
TreeFami | TF335284 |
Enzyme and pathway databases
BRENDAi | 3.4.21.76, 2681 |
PathwayCommonsi | P24158 |
Reactomei | R-HSA-140875, Common Pathway of Fibrin Clot Formation R-HSA-449836, Other interleukin signaling R-HSA-6798695, Neutrophil degranulation R-HSA-6803157, Antimicrobial peptides |
SABIO-RKi | P24158 |
SignaLinki | P24158 |
SIGNORi | P24158 |
Miscellaneous databases
BioGRID-ORCSi | 5657, 11 hits in 1069 CRISPR screens |
EvolutionaryTracei | P24158 |
GeneWikii | Proteinase_3 |
GenomeRNAii | 5657 |
Pharosi | P24158, Tchem |
PROi | PR:P24158 |
RNActi | P24158, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000196415, Expressed in bone marrow and 91 other tissues |
ExpressionAtlasi | P24158, baseline and differential |
Genevisiblei | P24158, HS |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PRTN3_HUMAN | |
Accessioni | P24158Primary (citable) accession number: P24158 Secondary accession number(s): P15637 Q9UQD8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | December 15, 1998 | |
Last modified: | May 25, 2022 | |
This is version 223 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families