UniProtKB - P24100 (CDKA1_ARATH)
Cyclin-dependent kinase A-1
CDKA-1
Functioni
Involved in the control of the cell cycle. Essential for both G1/S and G2/M (mitosis) phase transitions. Functions in cell morphogenesis as well as cell proliferation. Required for cell division (entry into mitosis) of the generative cell in male gametogenesis. Required to trigger guard mother cells (GMC) symmetric divisions at the late stage of stomatal development, probably via the regulation of G1 to S transition in the cell cycle. Required for the function of SPCH in entering the stomatal lineage (PubMed:25680231).
Promotes divisions in the guard cells (GCs) after the guard mother cells (GMC) symmetric division when in the presence of CYCD3-2 via the phosphorylation of SPCH (PubMed:24687979, PubMed:25680231).
5 PublicationsCatalytic activityi
- EC:2.7.11.222 Publications
- EC:2.7.11.222 Publications
- [DNA-directed RNA polymerase] + ATP = ADP + H+ + phospho-[DNA-directed RNA polymerase]2 PublicationsEC:2.7.11.232 Publications
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 33 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 127 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 18 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cyclin binding Source: GO_Central
- cyclin-dependent protein serine/threonine kinase activity Source: TAIR
- kinase activity Source: TAIR
- protein kinase activity Source: TAIR
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine kinase activity Source: RHEA
- RNA polymerase II CTD heptapeptide repeat kinase activity Source: UniProtKB-EC
GO - Biological processi
- asymmetric cell division Source: TAIR
- cytokinesis by cell plate formation Source: TAIR
- DNA endoreduplication Source: TAIR
- embryo development ending in seed dormancy Source: TAIR
- G1/S transition of mitotic cell cycle Source: GO_Central
- gametophyte development Source: TAIR
- guard mother cell cytokinesis Source: UniProtKB
- guard mother cell differentiation Source: UniProtKB
- meiotic cytokinesis Source: TAIR
- pollen development Source: UniProtKB
- positive regulation of cell population proliferation Source: TAIR
- protein phosphorylation Source: GO_Central
- regulation of cell cycle G1/S phase transition Source: UniProtKB
- regulation of G2/M transition of mitotic cell cycle Source: GO_Central
- regulation of gene expression Source: GO_Central
- regulation of meiotic cell cycle Source: GO_Central
- regulation of meiotic nuclear division Source: TAIR
- response to cold Source: TAIR
- signal transduction Source: GO_Central
- symmetric cell division Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.22, 399 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CDKA-11 Publication Synonyms:CDC22 Publications, CDC2A2 Publications Ordered Locus Names:At3g48750Imported ORF Names:T21J18.20Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G48750 |
TAIRi | locus:2099478, AT3G48750 |
Subcellular locationi
Cytoskeleton
- cortical microtubule, transverse to long axis Source: TAIR
- preprophase band Source: TAIR
Nucleus
- nucleus Source: TAIR
Other locations
- cyclin-dependent protein kinase holoenzyme complex Source: GO_Central
- cytoplasm Source: TAIR
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 14 | T → A: Increased kinase activity; when associated with F-15. 1 Publication | 1 | |
Mutagenesisi | 15 | Y → F: Abolishes phosphorylation by WEE1. Increased kinase activity; when associated with A-14. 2 Publications | 1 | |
Mutagenesisi | 146 | D → N: Decreased kinase activity and disturbed cell cycle. Reduced frequency of cell division during embryo development. Altered stomatal production. Interacts with SPCH. 3 Publications | 1 | |
Mutagenesisi | 156 | P → L: Decreased kinase activity and disturbed cell cycle. 1 Publication | 1 | |
Mutagenesisi | 161 | T → D: Strong reduction in kinase activity and ability to bind substrate. Strong reduction in plant growth. Sterile plants. 1 Publication | 1 | |
Mutagenesisi | 161 | T → V: Strong reduction in kinase activity and ability to bind substrate. 1 Publication | 1 | |
Mutagenesisi | 166 | T → I: Decreased kinase activity and disturbed cell cycle. 1 Publication | 1 | |
Mutagenesisi | 234 – 236 | Missing : No change in kinase activity, but disturbed cell cycle. Loss of interaction with CKS1. 1 Publication | 3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085749 | 1 – 294 | Cyclin-dependent kinase A-1Add BLAST | 294 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 15 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 161 | PhosphothreonineCombined sources1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P24100 |
PRIDEi | P24100 |
ProteomicsDBi | 220603 |
PTM databases
iPTMneti | P24100 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
ExpressionAtlasi | P24100, baseline and differential |
Genevisiblei | P24100, AT |
Interactioni
Subunit structurei
Interacts with CDT1A, CYCA2-3, CYCD2-1, CYCD3-1, CYCD4-1, CYCD4-2, CYCH1-1, CYCU1-1, CYCU2-1, CYCU2-2, CYCU3-1, CYCU4-1, CYCU4-2, CYCU4-3, CKS1, KRP2/ICK2, KRP3/ICK6, KRP4/ICK7, KRP6/ICK4, KRP7/ICK5, and C-terminal domain of KRP1/ICK1.
Interacts with WEE1 and TIF4A-1/EIF4A-1.
Interacts with PAS2; when phosphorylated at Tyr-15.
Interacts with SMR3, SMR4, SMR5, SMR6, SMR8 and At4g14310. Binds to CYCD3-2 (PubMed:24687979).
Component of a DREAM-like complex which modulates a variety of developmentally regulated genes and of the mitotic genes in proliferating and differentiated cells.
Interacts with MYB3R3 at later and with MYB3R4 at earlier stages of leaf development (PubMed:26069325). May interact with SPCH (PubMed:25680231).
28 PublicationsBinary interactionsi
P24100
GO - Molecular functioni
- cyclin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 9354, 107 interactors |
IntActi | P24100, 74 interactors |
STRINGi | 3702.AT3G48750.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 287 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 284 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0594, Eukaryota |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | P24100 |
OMAi | NNDVWPE |
OrthoDBi | 1010560at2759 |
PhylomeDBi | P24100 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDQYEKVEKI GEGTYGVVYK ARDKVTNETI ALKKIRLEQE DEGVPSTAIR
60 70 80 90 100
EISLLKEMQH SNIVKLQDVV HSEKRLYLVF EYLDLDLKKH MDSTPDFSKD
110 120 130 140 150
LHMIKTYLYQ ILRGIAYCHS HRVLHRDLKP QNLLIDRRTN SLKLADFGLA
160 170 180 190 200
RAFGIPVRTF THEVVTLWYR APEILLGSHH YSTPVDIWSV GCIFAEMISQ
210 220 230 240 250
KPLFPGDSEI DQLFKIFRIM GTPYEDTWRG VTSLPDYKSA FPKWKPTDLE
260 270 280 290
TFVPNLDPDG VDLLSKMLLM DPTKRINARA ALEHEYFKDL GGMP
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59198 mRNA Translation: AAA32831.1 S45387 mRNA Translation: AAB23643.1 X57839 mRNA Translation: CAA40971.1 D10850 Genomic DNA Translation: BAA01623.1 AL132963 Genomic DNA Translation: CAB87903.1 Sequence problems. CP002686 Genomic DNA Translation: AEE78452.1 AY090353 mRNA Translation: AAL91258.1 Frameshift. BT024706 mRNA Translation: ABD59044.1 AK226373 mRNA Translation: BAE98520.1 AY085153 mRNA Translation: AAM61706.1 |
PIRi | S23095 T49271 |
RefSeqi | NP_566911.1, NM_114734.4 |
Genome annotation databases
EnsemblPlantsi | AT3G48750.1; AT3G48750.1; AT3G48750 |
GeneIDi | 824036 |
Gramenei | AT3G48750.1; AT3G48750.1; AT3G48750 |
KEGGi | ath:AT3G48750 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59198 mRNA Translation: AAA32831.1 S45387 mRNA Translation: AAB23643.1 X57839 mRNA Translation: CAA40971.1 D10850 Genomic DNA Translation: BAA01623.1 AL132963 Genomic DNA Translation: CAB87903.1 Sequence problems. CP002686 Genomic DNA Translation: AEE78452.1 AY090353 mRNA Translation: AAL91258.1 Frameshift. BT024706 mRNA Translation: ABD59044.1 AK226373 mRNA Translation: BAE98520.1 AY085153 mRNA Translation: AAM61706.1 |
PIRi | S23095 T49271 |
RefSeqi | NP_566911.1, NM_114734.4 |
3D structure databases
AlphaFoldDBi | P24100 |
SMRi | P24100 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 9354, 107 interactors |
IntActi | P24100, 74 interactors |
STRINGi | 3702.AT3G48750.1 |
PTM databases
iPTMneti | P24100 |
Proteomic databases
PaxDbi | P24100 |
PRIDEi | P24100 |
ProteomicsDBi | 220603 |
Genome annotation databases
EnsemblPlantsi | AT3G48750.1; AT3G48750.1; AT3G48750 |
GeneIDi | 824036 |
Gramenei | AT3G48750.1; AT3G48750.1; AT3G48750 |
KEGGi | ath:AT3G48750 |
Organism-specific databases
Araporti | AT3G48750 |
TAIRi | locus:2099478, AT3G48750 |
Phylogenomic databases
eggNOGi | KOG0594, Eukaryota |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | P24100 |
OMAi | NNDVWPE |
OrthoDBi | 1010560at2759 |
PhylomeDBi | P24100 |
Enzyme and pathway databases
BRENDAi | 2.7.11.22, 399 |
Miscellaneous databases
PROi | PR:P24100 |
Gene expression databases
ExpressionAtlasi | P24100, baseline and differential |
Genevisiblei | P24100, AT |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CDKA1_ARATH | |
Accessioni | P24100Primary (citable) accession number: P24100 Secondary accession number(s): Q29Q50, Q8RX68, Q9M307 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | March 1, 1992 | |
Last modified: | May 25, 2022 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families