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Protein

Endochitinase B

Gene

CHN50

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Defense against chitin-containing fungal pathogens.

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei140Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase, Pathogenesis-related protein
Biological processCarbohydrate metabolism, Chitin degradation, Hypersensitive response, Plant defense, Polysaccharide degradation
LigandChitin-binding

Protein family/group databases

CAZyiCBM18 Carbohydrate-Binding Module Family 18
GH19 Glycoside Hydrolase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase B (EC:3.2.1.14)
Short name:
CHN-B
Gene namesi
Name:CHN50
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana
Proteomesi
  • UP000084051 Componenti: Genome assembly

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000000533224 – 317Endochitinase BAdd BLAST294
PropeptideiPRO_0000005333318 – 324Removed in mature formCurated7

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 41PROSITE-ProRule annotation
Disulfide bondi35 ↔ 47PROSITE-ProRule annotation
Disulfide bondi40 ↔ 54PROSITE-ProRule annotation
Disulfide bondi59 ↔ 63PROSITE-ProRule annotation
Modified residuei674-hydroxyproline1 Publication1
Modified residuei694-hydroxyproline1 Publication1
Disulfide bondi96 ↔ 158PROSITE-ProRule annotation
Disulfide bondi170 ↔ 178PROSITE-ProRule annotation
Disulfide bondi277 ↔ 309PROSITE-ProRule annotation

Post-translational modificationi

The 4-hydroxyproline residues are not glycosylated in this plant vacuolar protein.

Keywords - PTMi

Disulfide bond, Hydroxylation

Proteomic databases

PRIDEiP24091

Expressioni

Inductioni

By ethylene.

Structurei

3D structure databases

ProteinModelPortaliP24091
SMRiP24091
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 65Chitin-binding type-1PROSITE-ProRule annotationAdd BLAST42

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK20547

Family and domain databases

CDDicd00325 chitinase_glyco_hydro_19, 1 hit
Gene3Di3.30.60.10, 1 hit
InterProiView protein in InterPro
IPR001002 Chitin-bd_1
IPR018371 Chitin-binding_1_CS
IPR036861 Endochitinase-like_sf
IPR016283 Glyco_hydro_19
IPR000726 Glyco_hydro_19_cat
IPR023346 Lysozyme-like_dom_sf
PfamiView protein in Pfam
PF00187 Chitin_bind_1, 1 hit
PF00182 Glyco_hydro_19, 1 hit
PIRSFiPIRSF001060 Endochitinase, 1 hit
PRINTSiPR00451 CHITINBINDNG
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000609 Chitin_bd_1, 1 hit
SMARTiView protein in SMART
SM00270 ChtBD1, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
SSF57016 SSF57016, 1 hit
PROSITEiView protein in PROSITE
PS00026 CHIT_BIND_I_1, 1 hit
PS50941 CHIT_BIND_I_2, 1 hit
PS00773 CHITINASE_19_1, 1 hit
PS00774 CHITINASE_19_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24091-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLREFTALS SLLFSLLLLS ASAEQCGSQA GGARCASGLC CSKFGWCGNT
60 70 80 90 100
NDYCGPGNCQ SQCPGGPTPP GGGDLGSIIS SSMFDQMLKH RNDNACQGKG
110 120 130 140 150
FYSYNAFINA ARSFPGFGTS GDTTARKREI AAFFAQTSHE TTGGWATAPD
160 170 180 190 200
GPYAWGYCWL REQGSPGDYC TPSGQWPCAP GRKYFGRGPI QISHNYNYGP
210 220 230 240 250
CGRAIGVDLL NNPDLVATDP VISFKSALWF WMTPQSPKPS CHDVIIGRWQ
260 270 280 290 300
PSSADRAANR LPGFGVITNI INGGLECGRG TDSRVQDRIG FYRRYCSILG
310 320
VSPGDNLDCG NQRSFGNGLL VDTM
Length:324
Mass (Da):34,721
Last modified:March 1, 1992 - v1
Checksum:iFA65DC2113B33EB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51599 Genomic DNA Translation: CAA35945.1
X64519 Genomic DNA Translation: CAA45822.1
M15173 mRNA Translation: AAA34070.1
PIRiS20981
RefSeqiXP_016509308.1, XM_016653822.1
UniGeneiNta.4173

Genome annotation databases

GeneIDi107826794
KEGGinta:107826794

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51599 Genomic DNA Translation: CAA35945.1
X64519 Genomic DNA Translation: CAA45822.1
M15173 mRNA Translation: AAA34070.1
PIRiS20981
RefSeqiXP_016509308.1, XM_016653822.1
UniGeneiNta.4173

3D structure databases

ProteinModelPortaliP24091
SMRiP24091
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM18 Carbohydrate-Binding Module Family 18
GH19 Glycoside Hydrolase Family 19

Proteomic databases

PRIDEiP24091

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi107826794
KEGGinta:107826794

Phylogenomic databases

KOiK20547

Family and domain databases

CDDicd00325 chitinase_glyco_hydro_19, 1 hit
Gene3Di3.30.60.10, 1 hit
InterProiView protein in InterPro
IPR001002 Chitin-bd_1
IPR018371 Chitin-binding_1_CS
IPR036861 Endochitinase-like_sf
IPR016283 Glyco_hydro_19
IPR000726 Glyco_hydro_19_cat
IPR023346 Lysozyme-like_dom_sf
PfamiView protein in Pfam
PF00187 Chitin_bind_1, 1 hit
PF00182 Glyco_hydro_19, 1 hit
PIRSFiPIRSF001060 Endochitinase, 1 hit
PRINTSiPR00451 CHITINBINDNG
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000609 Chitin_bd_1, 1 hit
SMARTiView protein in SMART
SM00270 ChtBD1, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
SSF57016 SSF57016, 1 hit
PROSITEiView protein in PROSITE
PS00026 CHIT_BIND_I_1, 1 hit
PS50941 CHIT_BIND_I_2, 1 hit
PS00773 CHITINASE_19_1, 1 hit
PS00774 CHITINASE_19_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHI2_TOBAC
AccessioniPrimary (citable) accession number: P24091
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 10, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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