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Protein

Integrin alpha-L

Gene

Itgal

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4 (PubMed:2051027). Integrin alpha-L/beta-2 is also a receptor for F11R (By similarity). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:16234355, PubMed:24158516). Required for generation of common lymphoid progenitor cells in bone marrow, indicating the role in lymphopoiesis (PubMed:25108025). Integrin alpha-L/beta-2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.By similarity4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi466 – 474Sequence analysis9
Calcium bindingi528 – 536Sequence analysis9
Calcium bindingi588 – 596Sequence analysis9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIntegrin, Receptor
Biological processCell adhesion, Phagocytosis
LigandCalcium, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-202733 Cell surface interactions at the vascular wall
R-MMU-216083 Integrin cell surface interactions
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-L
Alternative name(s):
CD11 antigen-like family member A
Leukocyte adhesion glycoprotein LFA-1 alpha chain
Short name:
LFA-1A
Leukocyte function-associated molecule 1 alpha chain
Lymphocyte antigen 15
Short name:
Ly-15
CD_antigen: CD11a
Gene namesi
Name:Itgal
Synonyms:Lfa-1, Ly-15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96606 Itgal

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 1087ExtracellularSequence analysisAdd BLAST1064
Transmembranei1088 – 1108HelicalSequence analysisAdd BLAST21
Topological domaini1109 – 1163CytoplasmicSequence analysisAdd BLAST55

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show decreased cellularity in thymus but not spleen, and impaired early T cell development (PubMed:25108025). Obese mutant mice show decreased total number of T-cells, lower levels of neutrophil elastase and reduced cytotoxic T-cell proliferation in adipose tissue, as well as improved glucose tolerance and insulin resistance in comparison to obese wild type mice (PubMed:24158516).2 Publications

Chemistry databases

ChEMBLiCHEMBL1075188

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000001629324 – 1163Integrin alpha-LAdd BLAST1140

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 77By similarity
Glycosylationi86N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi108 ↔ 126By similarity
Disulfide bondi147 ↔ 199By similarity
Glycosylationi185N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi270N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi444N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi651 ↔ 705By similarity
Glycosylationi668N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi696N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi724N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi728N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi767 ↔ 773By similarity
Glycosylationi776N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi840 ↔ 856By similarity
Glycosylationi857N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi880N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi890N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi899N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi927N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi993 ↔ 1009By similarity
Disulfide bondi1017 ↔ 1048By similarity
Glycosylationi1056N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for the activation by some physiological agents.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP24063
MaxQBiP24063
PaxDbiP24063
PRIDEiP24063

PTM databases

iPTMnetiP24063
PhosphoSitePlusiP24063
SwissPalmiP24063

Expressioni

Tissue specificityi

Leukocytes.2 Publications

Gene expression databases

BgeeiENSMUSG00000030830 Expressed in 93 organ(s), highest expression level in mesenteric lymph node
CleanExiMM_ITGAL
ExpressionAtlasiP24063 baseline and differential
GenevisibleiP24063 MM

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Alpha-L associates with beta-2. Interacts with THBD.By similarity

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-3128 Integrin alphaL-beta2 complex
DIPiDIP-35643N
IntActiP24063, 4 interactors
STRINGi10090.ENSMUSP00000101913

Chemistry databases

BindingDBiP24063

Structurei

3D structure databases

ProteinModelPortaliP24063
SMRiP24063
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati28 – 79FG-GAP 1PROSITE-ProRule annotationAdd BLAST52
Repeati80 – 138FG-GAP 2PROSITE-ProRule annotationAdd BLAST59
Domaini153 – 325VWFAPROSITE-ProRule annotationAdd BLAST173
Repeati336 – 387FG-GAP 3PROSITE-ProRule annotationAdd BLAST52
Repeati390 – 443FG-GAP 4PROSITE-ProRule annotationAdd BLAST54
Repeati444 – 504FG-GAP 5PROSITE-ProRule annotationAdd BLAST61
Repeati505 – 561FG-GAP 6PROSITE-ProRule annotationAdd BLAST57
Repeati565 – 625FG-GAP 7PROSITE-ProRule annotationAdd BLAST61

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1111 – 1115GFFKR motif5

Domaini

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. The I-domain is necessary and sufficient for interaction with ICAM1 and F11R.By similarity

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPAP Eukaryota
ENOG410ZJIF LUCA
GeneTreeiENSGT00930000150854
HOGENOMiHOG000113114
HOVERGENiHBG006188
InParanoidiP24063

Family and domain databases

Gene3Di2.130.10.130, 1 hit
3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR013517 FG-GAP
IPR013519 Int_alpha_beta-p
IPR000413 Integrin_alpha
IPR013649 Integrin_alpha-2
IPR018184 Integrin_alpha_C_CS
IPR028994 Integrin_alpha_N
IPR032695 Integrin_dom_sf
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF01839 FG-GAP, 1 hit
PF00357 Integrin_alpha, 1 hit
PF08441 Integrin_alpha2, 1 hit
PF00092 VWA, 1 hit
PRINTSiPR01185 INTEGRINA
SMARTiView protein in SMART
SM00191 Int_alpha, 5 hits
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
SSF69179 SSF69179, 2 hits
PROSITEiView protein in PROSITE
PS51470 FG_GAP, 7 hits
PS00242 INTEGRIN_ALPHA, 1 hit
PS50234 VWFA, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P24063-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSFRIAGPRL LLLGLQLFAK AWSYNLDTRP TQSFLAQAGR HFGYQVLQIE
60 70 80 90 100
DGVVVGAPGE GDNTGGLYHC RTSSEFCQPV SLHGSNHTSK YLGMTLATDA
110 120 130 140 150
AKGSLLACDP GLSRTCDQNT YLSGLCYLFP QSLEGPMLQN RPAYQECMKG
160 170 180 190 200
KVDLVFLFDG SQSLDRKDFE KILEFMKDVM RKLSNTSYQF AAVQFSTDCR
210 220 230 240 250
TEFTFLDYVK QNKNPDVLLG SVQPMFLLTN TFRAINYVVA HVFKEESGAR
260 270 280 290 300
PDATKVLVII TDGEASDKGN ISAAHDITRY IIGIGKHFVS VQKQKTLHIF
310 320 330 340 350
ASEPVEEFVK ILDTFEKLKD LFTDLQRRIY AIEGTNRQDL TSFNMELSSS
360 370 380 390 400
GISADLSKGH AVVGAVGAKD WAGGFLDLRE DLQGATFVGQ EPLTSDVRGG
410 420 430 440 450
YLGYTVAWMT SRSSRPLLAA GAPRYQHVGQ VLLFQAPEAG GRWNQTQKIE
460 470 480 490 500
GTQIGSYFGG ELCSVDLDQD GEAELLLIGA PLFFGEQRGG RVFTYQRRQS
510 520 530 540 550
LFEMVSELQG DPGYPLGRFG AAITALTDIN GDRLTDVAVG APLEEQGAVY
560 570 580 590 600
IFNGKPGGLS PQPSQRIQGA QVFPGIRWFG RSIHGVKDLG GDRLADVVVG
610 620 630 640 650
AEGRVVVLSS RPVVDVVTEL SFSPEEIPVH EVECSYSARE EQKHGVKLKA
660 670 680 690 700
CFRIKPLTPQ FQGRLLANLS YTLQLDGHRM RSRGLFPDGS HELSGNTSIT
710 720 730 740 750
PDKSCLDFHF HFPICIQDLI SPINVSLNFS LLEEEGTPRD QKGRAMQPIL
760 770 780 790 800
RPSIHTVTKE IPFEKNCGED KKCEANLTLS SPARSGPLRL MSSASLAVEW
810 820 830 840 850
TLSNSGEDAY WVRLDLDFPR GLSFRKVEML QPHSRMPVSC EELTEGSSLL
860 870 880 890 900
TKTLKCNVSS PIFKAGQEVS LQVMFNTLLN SSWEDFVELN GTVHCENENS
910 920 930 940 950
SLQEDNSAAT HIPVLYPVNI LTKEQENSTL YISFTPKGPK TQQVQHVYQV
960 970 980 990 1000
RIQPSAYDHN MPTLEALVGV PWPHSEDPIT YTWSVQTDPL VTCHSEDLKR
1010 1020 1030 1040 1050
PSSEAEQPCL PGVQFRCPIV FRREILIQVT GTVELSKEIK ASSTLSLCSS
1060 1070 1080 1090 1100
LSVSFNSSKH FHLYGSKASE AQVLVKVDLI HEKEMLHVYV LSGIGGLVLL
1110 1120 1130 1140 1150
FLIFLALYKV GFFKRNLKEK MEADGGVPNG SPPEDTDPLA VPGEETKDMG
1160
CLEPSGRVTR TKA
Length:1,163
Mass (Da):128,328
Last modified:July 27, 2011 - v2
Checksum:iF19C786C4D33CC0C
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q5M7E9Q5M7_MOUSE
Integrin alpha-L
Itgal
1,162Annotation score:
E9QNL8E9QNL8_MOUSE
Integrin alpha-L
Itgal
1,161Annotation score:
D3Z627D3Z627_MOUSE
Integrin alpha-L
Itgal
1,201Annotation score:
D3YW95D3YW95_MOUSE
Integrin alpha-L
Itgal
765Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti972W → R in AAA39426 (PubMed:2051027).Curated1
Sequence conflicti978P → L in AAA39426 (PubMed:2051027).Curated1
Sequence conflicti1023R → W in AAA39426 (PubMed:2051027).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60778 mRNA Translation: AAA39426.1
AC133494 Genomic DNA No translation available.
PIRiI56126
UniGeneiMm.1618

Genome annotation databases

EnsembliENSMUST00000170971; ENSMUSP00000131847; ENSMUSG00000030830

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60778 mRNA Translation: AAA39426.1
AC133494 Genomic DNA No translation available.
PIRiI56126
UniGeneiMm.1618

3D structure databases

ProteinModelPortaliP24063
SMRiP24063
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-3128 Integrin alphaL-beta2 complex
DIPiDIP-35643N
IntActiP24063, 4 interactors
STRINGi10090.ENSMUSP00000101913

Chemistry databases

BindingDBiP24063
ChEMBLiCHEMBL1075188

PTM databases

iPTMnetiP24063
PhosphoSitePlusiP24063
SwissPalmiP24063

Proteomic databases

EPDiP24063
MaxQBiP24063
PaxDbiP24063
PRIDEiP24063

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000170971; ENSMUSP00000131847; ENSMUSG00000030830

Organism-specific databases

MGIiMGI:96606 Itgal

Phylogenomic databases

eggNOGiENOG410IPAP Eukaryota
ENOG410ZJIF LUCA
GeneTreeiENSGT00930000150854
HOGENOMiHOG000113114
HOVERGENiHBG006188
InParanoidiP24063

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-202733 Cell surface interactions at the vascular wall
R-MMU-216083 Integrin cell surface interactions
R-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

PROiPR:P24063
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030830 Expressed in 93 organ(s), highest expression level in mesenteric lymph node
CleanExiMM_ITGAL
ExpressionAtlasiP24063 baseline and differential
GenevisibleiP24063 MM

Family and domain databases

Gene3Di2.130.10.130, 1 hit
3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR013517 FG-GAP
IPR013519 Int_alpha_beta-p
IPR000413 Integrin_alpha
IPR013649 Integrin_alpha-2
IPR018184 Integrin_alpha_C_CS
IPR028994 Integrin_alpha_N
IPR032695 Integrin_dom_sf
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF01839 FG-GAP, 1 hit
PF00357 Integrin_alpha, 1 hit
PF08441 Integrin_alpha2, 1 hit
PF00092 VWA, 1 hit
PRINTSiPR01185 INTEGRINA
SMARTiView protein in SMART
SM00191 Int_alpha, 5 hits
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
SSF69179 SSF69179, 2 hits
PROSITEiView protein in PROSITE
PS51470 FG_GAP, 7 hits
PS00242 INTEGRIN_ALPHA, 1 hit
PS50234 VWFA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiITAL_MOUSE
AccessioniPrimary (citable) accession number: P24063
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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