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Protein

Cyclin-dependent kinase 1-B

Gene

cdk1-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Activity regulationi

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei128Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22 6725

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1-B (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDK1-B
Alternative name(s):
Cell division control protein 2 homolog 2
Cell division control protein 2-B
Cell division protein kinase 1
p34 protein kinase 2
Gene namesi
Name:cdk1-b
Synonyms:cdc2, cdc2x1.2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6254942 cdk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857361 – 302Cyclin-dependent kinase 1-BAdd BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphothreonineBy similarity1
Modified residuei15Phosphotyrosine; by wee1 and wee22 Publications1
Modified residuei161Phosphothreonine; by cak1 Publication1
Modified residuei277PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Tyr-15 by wee1 and wee2 inhibits the protein kinase activity and acts negative regulator of entry into mitosis (G2 to M transition).3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP24033

PTM databases

iPTMnetiP24033

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with spdya.3 Publications

Structurei

3D structure databases

ProteinModelPortaliP24033
SMRiP24033
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 287Protein kinasePROSITE-ProRule annotationAdd BLAST284

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG014652
KOiK02087

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P24033-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDEYTKIEKI GEGTYGVVYK GRHKATGQVV AMKKIRLENE EEGVPSTAIR
60 70 80 90 100
EISLLKELQH PNIVCLLDVL MQDSRLYLIF EFLSMDLKKY LDSIPSGQYI
110 120 130 140 150
DTMLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDNKG VIKLADFGLA
160 170 180 190 200
RAFGIPVRVY THEVVTLWYR ASEVLLGSVR YSTPVDVWSV GTIFAEIATK
210 220 230 240 250
KPLFHGDSEI DQLFRIFRSL GTPNNEVWPE VESLQDYKNT FPKWKGGSLS
260 270 280 290 300
SNVKNIDEDG LDLLSKMLVY DPAKRISARK AMLHPYFDDL DKSSLPANQI

RN
Length:302
Mass (Da):34,532
Last modified:May 30, 2006 - v2
Checksum:iFA9DC156392D7CBC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87L → V in AAA63562 (PubMed:1377775).Curated1
Sequence conflicti123R → G in AAA63562 (PubMed:1377775).Curated1
Sequence conflicti172S → P in AAA63562 (PubMed:1377775).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60681 mRNA Translation: AAA63562.1
BC054146 mRNA Translation: AAH54146.1
PIRiB44349
RefSeqiNP_001080093.1, NM_001086624.1
UniGeneiXl.3815

Genome annotation databases

GeneIDi379785
KEGGixla:379785

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60681 mRNA Translation: AAA63562.1
BC054146 mRNA Translation: AAH54146.1
PIRiB44349
RefSeqiNP_001080093.1, NM_001086624.1
UniGeneiXl.3815

3D structure databases

ProteinModelPortaliP24033
SMRiP24033
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP24033

Proteomic databases

PRIDEiP24033

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi379785
KEGGixla:379785

Organism-specific databases

CTDi379785
XenbaseiXB-GENE-6254942 cdk1

Phylogenomic databases

HOVERGENiHBG014652
KOiK02087

Enzyme and pathway databases

BRENDAi2.7.11.22 6725

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCDK1B_XENLA
AccessioniPrimary (citable) accession number: P24033
Secondary accession number(s): Q7SZ44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: May 30, 2006
Last modified: October 10, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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