sli

Functionⁱ

A short-range repellent, controlling axon crossing of the midline and a long-range chemorepellent, controlling mesoderm migration and patterning away from the midline. May interact with extracellular matrix molecules. Repulsive ligand for the guidance receptor roundabout (robo) and prevents inappropriate midline crossing by Robo-expressing axons.

4 Publications

GO - Molecular functionⁱ

calcium ion binding Source: InterPro
heparin binding
Source: FlyBase
Inferred from direct assayⁱ
- "A molecular mechanism for the heparan sulfate dependence of slit-robo signaling."
  Hussain S.A., Piper M., Fukuhara N., Strochlic L., Cho G., Howitt J.A., Ahmed Y., Powell A.K., Turnbull J.E., Holt C.E., Hohenester E.
  J Biol Chem 281:39693-39698(2006) [PubMed] [Europe PMC] [Abstract]
Roundabout binding
Source: FlyBase
Inferred from physical interactionⁱ
- "A molecular mechanism for the heparan sulfate dependence of slit-robo signaling."
  Hussain S.A., Piper M., Fukuhara N., Strochlic L., Cho G., Howitt J.A., Ahmed Y., Powell A.K., Turnbull J.E., Holt C.E., Hohenester E.
  J Biol Chem 281:39693-39698(2006) [PubMed] [Europe PMC] [Abstract]
- "Structural and functional analysis of slit and heparin binding to immunoglobulin-like domains 1 and 2 of Drosophila Robo."
  Fukuhara N., Howitt J.A., Hussain S.A., Hohenester E.
  J Biol Chem 283:16226-16234(2008) [PubMed] [Europe PMC] [Abstract]

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Developmental protein
Biological process	Differentiation, Neurogenesis

Enzyme and pathway databases

Reactomeⁱ	R-DME-376176, Signaling by ROBO receptors R-DME-428890, Role of ABL in ROBO-SLIT signaling
SignaLinkⁱ	P24014

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protein slit Short name: dSlit Cleaved into the following 2 chains: Protein slit N-product Protein slit C-product
Gene namesⁱ	Name:sli ORF Names:CG43758
Organismⁱ	Drosophila melanogaster (Fruit fly)
Taxonomic identifierⁱ	7227 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophila › Sophophora › melanogaster group › melanogaster subgroup
Proteomesⁱ	UP000000803 Componentⁱ: Chromosome 2R

Organism-specific databases

Drosophila genome database More...FlyBaseⁱ	FBgn0264089, sli
VEuPathDBⁱ	VectorBase:FBgn0264089

Keywords - Cellular componentⁱ

Secreted

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Flies lacking sli exhibit disruption of the developing midline cells and the commissural axon pathways.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – 36	Add BLAST	36
ChainⁱPRO_0000007719	37 – 1504	Protein slitAdd BLAST	1468
ChainⁱPRO_0000007720	37 – 1135	Protein slit N-productBy similarityAdd BLAST	1099
ChainⁱPRO_0000007721	1136 – 1504	Protein slit C-productBy similarityAdd BLAST	369

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	111	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	231	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	381	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	459	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	543 ↔ 549	1 Publication
Disulfide bondⁱ	547 ↔ 556	1 Publication
Disulfide bondⁱ	657 ↔ 733	1 Publication
Disulfide bondⁱ	681 ↔ 704	1 Publication
Disulfide bondⁱ	683 ↔ 725	1 Publication
Glycosylationⁱ	807	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	812	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	935 ↔ 946	By similarity
Disulfide bondⁱ	940 ↔ 956	By similarity
Disulfide bondⁱ	958 ↔ 967	By similarity
Disulfide bondⁱ	974 ↔ 985	By similarity
Disulfide bondⁱ	979 ↔ 995	By similarity
Glycosylationⁱ	982	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	997 ↔ 1006	By similarity
Disulfide bondⁱ	1013 ↔ 1025	By similarity
Disulfide bondⁱ	1019 ↔ 1034	By similarity
Glycosylationⁱ	1022	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	1036 ↔ 1045	By similarity
Disulfide bondⁱ	1052 ↔ 1065	By similarity
Disulfide bondⁱ	1059 ↔ 1074	By similarity
Disulfide bondⁱ	1076 ↔ 1085	By similarity
Glycosylationⁱ	1084	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	1092 ↔ 1103	By similarity
Disulfide bondⁱ	1097 ↔ 1112	By similarity
Disulfide bondⁱ	1114 ↔ 1123	By similarity
Disulfide bondⁱ	1139 ↔ 1149	By similarity
Disulfide bondⁱ	1144 ↔ 1161	By similarity
Disulfide bondⁱ	1163 ↔ 1172	By similarity
Glycosylationⁱ	1183	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	1199	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	1267	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	1316	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	1323 ↔ 1349	By similarity
Disulfide bondⁱ	1381 ↔ 1392	By similarity
Disulfide bondⁱ	1386 ↔ 1404	By similarity
Disulfide bondⁱ	1406 ↔ 1415	By similarity
Disulfide bondⁱ	1433 ↔ 1467	By similarity
Disulfide bondⁱ	1447 ↔ 1481	By similarity
Disulfide bondⁱ	1458 ↔ 1497	By similarity
Disulfide bondⁱ	1462 ↔ 1499	By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	1135 – 1136	CleavageBy similarity		2

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbⁱ	P24014

PaxDbⁱ

P24014

PTM databases

GlyGenⁱ	P24014, 13 sites

GlyGenⁱ

P24014, 13 sites

Expressionⁱ

Tissue specificityⁱ

In embryos, highest expression occurs around the midline glia and low expression is observed around CNS axons lateral to the midline. Expression can be seen on the commissural axons traversing the glial cells but it is absent from the cell bodies of these neurons.3 Publications

Gene expression databases

Bgeeⁱ	FBgn0264089, Expressed in central nervous system and 21 other tissues
ExpressionAtlasⁱ	P24014, baseline and differential
Genevisibleⁱ	P24014, DM

Interactionⁱ

Subunit structureⁱ

Interacts with robo.

1 Publication

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGRIDⁱ	62473, 41 interactors
IntActⁱ	P24014, 7 interactors
STRINGⁱ	7227.FBpp0303575

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	547 – 550	Combined sources	4
Beta strandⁱ	553 – 555	Combined sources	3
Beta strandⁱ	573 – 576	Combined sources	4
Helixⁱ	591 – 593	Combined sources	3
Beta strandⁱ	599 – 601	Combined sources	3
Turnⁱ	612 – 617	Combined sources	6
Beta strandⁱ	623 – 625	Combined sources	3
Beta strandⁱ	636 – 639	Combined sources	4
Beta strandⁱ	647 – 649	Combined sources	3
Beta strandⁱ	671 – 673	Combined sources	3
Helixⁱ	683 – 685	Combined sources	3
Helixⁱ	686 – 695	Combined sources	10
Helixⁱ	699 – 701	Combined sources	3
Turnⁱ	708 – 712	Combined sources	5
Helixⁱ	715 – 717	Combined sources	3
Turnⁱ	720 – 722	Combined sources	3

Show more details

3D structure databases

SMRⁱ	P24014
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P24014

EvolutionaryTraceⁱ

P24014

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	64 – 100	LRRNT 1Add BLAST	37
Repeatⁱ	101 – 122	LRR 1Add BLAST	22
Repeatⁱ	125 – 146	LRR 2Add BLAST	22
Repeatⁱ	149 – 170	LRR 3Add BLAST	22
Repeatⁱ	173 – 194	LRR 4Add BLAST	22
Repeatⁱ	197 – 218	LRR 5Add BLAST	22
Repeatⁱ	221 – 242	LRR 6Add BLAST	22
Domainⁱ	254 – 304	LRRCT 1Add BLAST	51
Domainⁱ	310 – 346	LRRNT 2Add BLAST	37
Repeatⁱ	347 – 368	LRR 7Add BLAST	22
Repeatⁱ	371 – 392	LRR 8Add BLAST	22
Repeatⁱ	395 – 416	LRR 9Add BLAST	22
Repeatⁱ	419 – 440	LRR 10Add BLAST	22
Repeatⁱ	443 – 464	LRR 11Add BLAST	22
Domainⁱ	476 – 526	LRRCT 2Add BLAST	51
Domainⁱ	534 – 570	LRRNT 3Add BLAST	37
Repeatⁱ	571 – 592	LRR 12Add BLAST	22
Repeatⁱ	596 – 617	LRR 13Add BLAST	22
Repeatⁱ	620 – 641	LRR 14Add BLAST	22
Repeatⁱ	644 – 665	LRR 15Add BLAST	22
Domainⁱ	677 – 727	LRRCT 3Add BLAST	51
Domainⁱ	730 – 766	LRRNT 4Add BLAST	37
Repeatⁱ	767 – 788	LRR 16Add BLAST	22
Repeatⁱ	791 – 812	LRR 17Add BLAST	22
Repeatⁱ	815 – 836	LRR 18Add BLAST	22
Repeatⁱ	839 – 860	LRR 19Add BLAST	22
Domainⁱ	872 – 922	LRRCT 4Add BLAST	51
Domainⁱ	931 – 968	EGF-like 1PROSITE-ProRule annotationAdd BLAST	38
Domainⁱ	970 – 1007	EGF-like 2PROSITE-ProRule annotationAdd BLAST	38
Domainⁱ	1009 – 1046	EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST	38
Domainⁱ	1048 – 1086	EGF-like 4PROSITE-ProRule annotationAdd BLAST	39
Domainⁱ	1088 – 1124	EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd BLAST	37
Domainⁱ	1135 – 1173	EGF-like 6PROSITE-ProRule annotationAdd BLAST	39
Domainⁱ	1176 – 1349	Laminin G-likePROSITE-ProRule annotationAdd BLAST	174
Domainⁱ	1377 – 1416	EGF-like 7PROSITE-ProRule annotationAdd BLAST	40
Domainⁱ	1433 – 1504	CTCKPROSITE-ProRule annotationAdd BLAST	72

Keywords - Domainⁱ

EGF-like domain, Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGⁱ	KOG4237, Eukaryota
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00940000167217
InParanoidⁱ	P24014
OMAⁱ	ETKCQNN
PhylomeDBⁱ	P24014

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00110, LamG, 1 hit
Gene3Dⁱ	3.80.10.10, 5 hits
InterProⁱ	View protein in InterPro IPR013320, ConA-like_dom_sf IPR000483, Cys-rich_flank_reg_C IPR006207, Cys_knot_C IPR001881, EGF-like_Ca-bd_dom IPR013032, EGF-like_CS IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR009030, Growth_fac_rcpt_cys_sf IPR001791, Laminin_G IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR000372, LRRNT
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00008, EGF, 4 hits PF12661, hEGF, 1 hit PF00054, Laminin_G_1, 1 hit PF13855, LRR_8, 6 hits PF01463, LRRCT, 4 hits PF01462, LRRNT, 4 hits
SMARTⁱ	View protein in SMART SM00041, CT, 1 hit SM00181, EGF, 7 hits SM00179, EGF_CA, 6 hits SM00282, LamG, 1 hit SM00369, LRR_TYP, 16 hits SM00082, LRRCT, 4 hits SM00013, LRRNT, 4 hits
SUPFAMⁱ	SSF49899, SSF49899, 1 hit SSF57184, SSF57184, 1 hit
PROSITEⁱ	View protein in PROSITE PS00010, ASX_HYDROXYL, 3 hits PS01185, CTCK_1, 1 hit PS01225, CTCK_2, 1 hit PS00022, EGF_1, 7 hits PS01186, EGF_2, 5 hits PS50026, EGF_3, 7 hits PS01187, EGF_CA, 2 hits PS50025, LAM_G_DOMAIN, 1 hit PS51450, LRR, 20 hits

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform C (identifier: P24014-1) [UniParc]FASTA Add to basket

Also known as: E

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPSRTTLM PPPFRLQLRL LILPILLLLR HDAVHAEPYS GGFGSSAVSS 
        60         70         80         90        100
GGLGSVGIHI PGGGVGVITE ARCPRVCSCT GLNVDCSHRG LTSVPRKISA 
       110        120        130        140        150
DVERLELQGN NLTVIYETDF QRLTKLRMLQ LTDNQIHTIE RNSFQDLVSL 
       160        170        180        190        200
ERLRLNNNRL KAIPENFVTS SASLLRLDIS NNVITTVGRR VFKGAQSLRS 
       210        220        230        240        250
LQLDNNQITC LDEHAFKGLV ELEILTLNNN NLTSLPHNIF GGLGRLRALR 
       260        270        280        290        300
LSDNPFACDC HLSWLSRFLR SATRLAPYTR CQSPSQLKGQ NVADLHDQEF 
       310        320        330        340        350
KCSGLTEHAP MECGAENSCP HPCRCADGIV DCREKSLTSV PVTLPDDTTE 
       360        370        380        390        400
LRLEQNFITE LPPKSFSSFR RLRRIDLSNN NISRIAHDAL SGLKQLTTLV 
       410        420        430        440        450
LYGNKIKDLP SGVFKGLGSL QLLLLNANEI SCIRKDAFRD LHSLSLLSLY 
       460        470        480        490        500
DNNIQSLANG TFDAMKSIKT VHLAKNPFIC DCNLRWLADY LHKNPIETSG 
       510        520        530        540        550
ARCESPKRMH RRRIESLREE KFKCSWDELR MKLSGECRMD SDCPAMCHCE 
       560        570        580        590        600
GTTVDCTGRG LKEIPRDIPL HTTELLLNDN ELGRISSDGL FGRLPHLVKL 
       610        620        630        640        650
ELKRNQLTGI EPNAFEGASH IQELQLGENK IKEISNKMFL GLHQLKTLNL 
       660        670        680        690        700
YDNQISCVMP GSFEHLNSLT SLNLASNPFN CNCHLAWFAE WLRKKSLNGG 
       710        720        730        740        750
AARCGAPSKV RDVQIKDLPH SEFKCSSENS EGCLGDGYCP PSCTCTGTVV 
       760        770        780        790        800
RCSRNQLKEI PRGIPAETSE LYLESNEIEQ IHYERIRHLR SLTRLDLSNN 
       810        820        830        840        850
QITILSNYTF ANLTKLSTLI ISYNKLQCLQ RHALSGLNNL RVLSLHGNRI 
       860        870        880        890        900
SMLPEGSFED LKSLTHIALG SNPLYCDCGL KWFSDWIKLD YVEPGIARCA 
       910        920        930        940        950
EPEQMKDKLI LSTPSSSFVC RGRVRNDILA KCNACFEQPC QNQAQCVALP 
       960        970        980        990       1000
QREYQCLCQP GYHGKHCEFM IDACYGNPCR NNATCTVLEE GRFSCQCAPG 
      1010       1020       1030       1040       1050
YTGARCETNI DDCLGEIKCQ NNATCIDGVE SYKCECQPGF SGEFCDTKIQ 
      1060       1070       1080       1090       1100
FCSPEFNPCA NGAKCMDHFT HYSCDCQAGF HGTNCTDNID DCQNHMCQNG 
      1110       1120       1130       1140       1150
GTCVDGINDY QCRCPDDYTG KYCEGHNMIS MMYPQTSPCQ NHECKHGVCF 
      1160       1170       1180       1190       1200
QPNAQGSDYL CRCHPGYTGK WCEYLTSISF VHNNSFVELE PLRTRPEANV 
      1210       1220       1230       1240       1250
TIVFSSAEQN GILMYDGQDA HLAVELFNGR IRVSYDVGNH PVSTMYSFEM 
      1260       1270       1280       1290       1300
VADGKYHAVE LLAIKKNFTL RVDRGLARSI INEGSNDYLK LTTPMFLGGL 
      1310       1320       1330       1340       1350
PVDPAQQAYK NWQIRNLTSF KGCMKEVWIN HKLVDFGNAQ RQQKITPGCA 
      1360       1370       1380       1390       1400
LLEGEQQEEE DDEQDFMDET PHIKEEPVDP CLENKCRRGS RCVPNSNARD 
      1410       1420       1430       1440       1450
GYQCKCKHGQ RGRYCDQGEG STEPPTVTAA STCRKEQVRE YYTENDCRSR 
      1460       1470       1480       1490       1500
QPLKYAKCVG GCGNQCCAAK IVRRRKVRMV CSNNRKYIKN LDIVRKCGCT 

KKCY

Length:1,504

Mass (Da):168,599

Last modified:February 28, 2003 - v2

Checksum:ⁱ836A3F5022BF234F

GO

Isoform A (identifier: P24014-2) [UniParc]FASTA Add to basket

Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
152-175: Missing.

« Hide

        10         20         30         40         50
MAAPSRTTLM PPPFRLQLRL LILPILLLLR HDAVHAEPYS GGFGSSAVSS 
        60         70         80         90        100
GGLGSVGIHI PGGGVGVITE ARCPRVCSCT GLNVDCSHRG LTSVPRKISA 
       110        120        130        140        150
DVERLELQGN NLTVIYETDF QRLTKLRMLQ LTDNQIHTIE RNSFQDLVSL 
       160        170        180        190        200
ERLDISNNVI TTVGRRVFKG AQSLRSLQLD NNQITCLDEH AFKGLVELEI 
       210        220        230        240        250
LTLNNNNLTS LPHNIFGGLG RLRALRLSDN PFACDCHLSW LSRFLRSATR 
       260        270        280        290        300
LAPYTRCQSP SQLKGQNVAD LHDQEFKCSG LTEHAPMECG AENSCPHPCR 
       310        320        330        340        350
CADGIVDCRE KSLTSVPVTL PDDTTELRLE QNFITELPPK SFSSFRRLRR 
       360        370        380        390        400
IDLSNNNISR IAHDALSGLK QLTTLVLYGN KIKDLPSGVF KGLGSLQLLL 
       410        420        430        440        450
LNANEISCIR KDAFRDLHSL SLLSLYDNNI QSLANGTFDA MKSIKTVHLA 
       460        470        480        490        500
KNPFICDCNL RWLADYLHKN PIETSGARCE SPKRMHRRRI ESLREEKFKC 
       510        520        530        540        550
SWDELRMKLS GECRMDSDCP AMCHCEGTTV DCTGRGLKEI PRDIPLHTTE 
       560        570        580        590        600
LLLNDNELGR ISSDGLFGRL PHLVKLELKR NQLTGIEPNA FEGASHIQEL 
       610        620        630        640        650
QLGENKIKEI SNKMFLGLHQ LKTLNLYDNQ ISCVMPGSFE HLNSLTSLNL 
       660        670        680        690        700
ASNPFNCNCH LAWFAEWLRK KSLNGGAARC GAPSKVRDVQ IKDLPHSEFK 
       710        720        730        740        750
CSSENSEGCL GDGYCPPSCT CTGTVVRCSR NQLKEIPRGI PAETSELYLE 
       760        770        780        790        800
SNEIEQIHYE RIRHLRSLTR LDLSNNQITI LSNYTFANLT KLSTLIISYN 
       810        820        830        840        850
KLQCLQRHAL SGLNNLRVLS LHGNRISMLP EGSFEDLKSL THIALGSNPL 
       860        870        880        890        900
YCDCGLKWFS DWIKLDYVEP GIARCAEPEQ MKDKLILSTP SSSFVCRGRV 
       910        920        930        940        950
RNDILAKCNA CFEQPCQNQA QCVALPQREY QCLCQPGYHG KHCEFMIDAC 
       960        970        980        990       1000
YGNPCRNNAT CTVLEEGRFS CQCAPGYTGA RCETNIDDCL GEIKCQNNAT 
      1010       1020       1030       1040       1050
CIDGVESYKC ECQPGFSGEF CDTKIQFCSP EFNPCANGAK CMDHFTHYSC 
      1060       1070       1080       1090       1100
DCQAGFHGTN CTDNIDDCQN HMCQNGGTCV DGINDYQCRC PDDYTGKYCE 
      1110       1120       1130       1140       1150
GHNMISMMYP QTSPCQNHEC KHGVCFQPNA QGSDYLCRCH PGYTGKWCEY 
      1160       1170       1180       1190       1200
LTSISFVHNN SFVELEPLRT RPEANVTIVF SSAEQNGILM YDGQDAHLAV 
      1210       1220       1230       1240       1250
ELFNGRIRVS YDVGNHPVST MYSFEMVADG KYHAVELLAI KKNFTLRVDR 
      1260       1270       1280       1290       1300
GLARSIINEG SNDYLKLTTP MFLGGLPVDP AQQAYKNWQI RNLTSFKGCM 
      1310       1320       1330       1340       1350
KEVWINHKLV DFGNAQRQQK ITPGCALLEG EQQEEEDDEQ DFMDETPHIK 
      1360       1370       1380       1390       1400
EEPVDPCLEN KCRRGSRCVP NSNARDGYQC KCKHGQRGRY CDQGEGSTEP 
      1410       1420       1430       1440       1450
PTVTAASTCR KEQVREYYTE NDCRSRQPLK YAKCVGGCGN QCCAAKIVRR 
      1460       1470       1480
RKVRMVCSNN RKYIKNLDIV RKCGCTKKCY

Show »

Length:1,480

Mass (Da):165,890

Checksum:ⁱF456BC617D8453ED

GO

Isoform B (identifier: P24014-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
152-175: Missing.
1418-1428: Missing.

« Hide

        10         20         30         40         50
MAAPSRTTLM PPPFRLQLRL LILPILLLLR HDAVHAEPYS GGFGSSAVSS 
        60         70         80         90        100
GGLGSVGIHI PGGGVGVITE ARCPRVCSCT GLNVDCSHRG LTSVPRKISA 
       110        120        130        140        150
DVERLELQGN NLTVIYETDF QRLTKLRMLQ LTDNQIHTIE RNSFQDLVSL 
       160        170        180        190        200
ERLDISNNVI TTVGRRVFKG AQSLRSLQLD NNQITCLDEH AFKGLVELEI 
       210        220        230        240        250
LTLNNNNLTS LPHNIFGGLG RLRALRLSDN PFACDCHLSW LSRFLRSATR 
       260        270        280        290        300
LAPYTRCQSP SQLKGQNVAD LHDQEFKCSG LTEHAPMECG AENSCPHPCR 
       310        320        330        340        350
CADGIVDCRE KSLTSVPVTL PDDTTELRLE QNFITELPPK SFSSFRRLRR 
       360        370        380        390        400
IDLSNNNISR IAHDALSGLK QLTTLVLYGN KIKDLPSGVF KGLGSLQLLL 
       410        420        430        440        450
LNANEISCIR KDAFRDLHSL SLLSLYDNNI QSLANGTFDA MKSIKTVHLA 
       460        470        480        490        500
KNPFICDCNL RWLADYLHKN PIETSGARCE SPKRMHRRRI ESLREEKFKC 
       510        520        530        540        550
SWDELRMKLS GECRMDSDCP AMCHCEGTTV DCTGRGLKEI PRDIPLHTTE 
       560        570        580        590        600
LLLNDNELGR ISSDGLFGRL PHLVKLELKR NQLTGIEPNA FEGASHIQEL 
       610        620        630        640        650
QLGENKIKEI SNKMFLGLHQ LKTLNLYDNQ ISCVMPGSFE HLNSLTSLNL 
       660        670        680        690        700
ASNPFNCNCH LAWFAEWLRK KSLNGGAARC GAPSKVRDVQ IKDLPHSEFK 
       710        720        730        740        750
CSSENSEGCL GDGYCPPSCT CTGTVVRCSR NQLKEIPRGI PAETSELYLE 
       760        770        780        790        800
SNEIEQIHYE RIRHLRSLTR LDLSNNQITI LSNYTFANLT KLSTLIISYN 
       810        820        830        840        850
KLQCLQRHAL SGLNNLRVLS LHGNRISMLP EGSFEDLKSL THIALGSNPL 
       860        870        880        890        900
YCDCGLKWFS DWIKLDYVEP GIARCAEPEQ MKDKLILSTP SSSFVCRGRV 
       910        920        930        940        950
RNDILAKCNA CFEQPCQNQA QCVALPQREY QCLCQPGYHG KHCEFMIDAC 
       960        970        980        990       1000
YGNPCRNNAT CTVLEEGRFS CQCAPGYTGA RCETNIDDCL GEIKCQNNAT 
      1010       1020       1030       1040       1050
CIDGVESYKC ECQPGFSGEF CDTKIQFCSP EFNPCANGAK CMDHFTHYSC 
      1060       1070       1080       1090       1100
DCQAGFHGTN CTDNIDDCQN HMCQNGGTCV DGINDYQCRC PDDYTGKYCE 
      1110       1120       1130       1140       1150
GHNMISMMYP QTSPCQNHEC KHGVCFQPNA QGSDYLCRCH PGYTGKWCEY 
      1160       1170       1180       1190       1200
LTSISFVHNN SFVELEPLRT RPEANVTIVF SSAEQNGILM YDGQDAHLAV 
      1210       1220       1230       1240       1250
ELFNGRIRVS YDVGNHPVST MYSFEMVADG KYHAVELLAI KKNFTLRVDR 
      1260       1270       1280       1290       1300
GLARSIINEG SNDYLKLTTP MFLGGLPVDP AQQAYKNWQI RNLTSFKGCM 
      1310       1320       1330       1340       1350
KEVWINHKLV DFGNAQRQQK ITPGCALLEG EQQEEEDDEQ DFMDETPHIK 
      1360       1370       1380       1390       1400
EEPVDPCLEN KCRRGSRCVP NSNARDGYQC KCKHGQRGRY CDQAASTCRK 
      1410       1420       1430       1440       1450
EQVREYYTEN DCRSRQPLKY AKCVGGCGNQ CCAAKIVRRR KVRMVCSNNR 
      1460 
KYIKNLDIVR KCGCTKKCY

Show »

Length:1,469

Mass (Da):164,834

Checksum:ⁱ14DBA99CE1DAEAC4

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A0B4KES0	A0A0B4KES0_DROME		Slit, isoform F Slit, isoform F	sli CG33464, CG8355, CT21700, CT37068, Dmel\CG43758	2,157	Annotation score:

Sequence cautionⁱ

The sequence AAA72722 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	350 – 351	EL → DV in CAA37910 (PubMed:2176636).Curated	2
Sequence conflictⁱ	350 – 351	EL → DV in AAD26567 (PubMed:10102267).Curated	2
Sequence conflictⁱ	421	Q → R in CAA37910 (PubMed:2176636).Curated	1
Sequence conflictⁱ	468	I → M in CAA37910 (PubMed:2176636).Curated	1
Sequence conflictⁱ	527	D → G in CAA37910 (PubMed:2176636).Curated	1
Sequence conflictⁱ	560	G → R in CAA37910 (PubMed:2176636).Curated	1
Sequence conflictⁱ	691 – 692	WL → CV in CAA37910 (PubMed:2176636).Curated	2
Sequence conflictⁱ	751	R → A in CAA37910 (PubMed:2176636).Curated	1
Sequence conflictⁱ	843	L → V in CAA37910 (PubMed:2176636).Curated	1
Sequence conflictⁱ	1207 – 1208	AE → G in AAA72722 (PubMed:3144436).Curated	2
Sequence conflictⁱ	1429 – 1435	AASTCRK → GTYIYHA in AAA72722 (PubMed:3144436).Curated	7

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_001408	152 – 175	Missing in isoform A and isoform B. 1 PublicationAdd BLAST		24
Alternative sequenceⁱVSP_001409	1418 – 1428	Missing in isoform B. 1 PublicationAdd BLAST		11

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X53959 mRNA Translation: CAA37910.1 AF126540 mRNA Translation: AAD26567.1 AE013599 Genomic DNA Translation: AAF58097.1 AE013599 Genomic DNA Translation: AAF58098.1 AE013599 Genomic DNA Translation: AAM70966.1 AE013599 Genomic DNA Translation: ABV53816.1 AE013599 Genomic DNA Translation: ABV53817.1 M23543 Genomic DNA Translation: AAA72722.1 Different initiation.
PIRⁱ	A36665 B36665
NCBI Reference Sequences More...RefSeqⁱ	NP_001097333.1, NM_001103863.3 [P24014-2] NP_001097334.1, NM_001103864.3 [P24014-1] NP_476727.1, NM_057379.4 [P24014-1] NP_476728.1, NM_057380.4 [P24014-2] NP_476729.1, NM_057381.4 [P24014-3]

Genome annotation databases

EnsemblMetazoaⁱ	FBtr0330729; FBpp0303573; FBgn0264089 [P24014-2] FBtr0330730; FBpp0303574; FBgn0264089 [P24014-3] FBtr0330731; FBpp0303575; FBgn0264089 [P24014-1] FBtr0330732; FBpp0303576; FBgn0264089 [P24014-2] FBtr0330733; FBpp0303577; FBgn0264089 [P24014-1]
GeneIDⁱ	36746
KEGGⁱ	dme:Dmel_CG43758
UCSC genome browser More...UCSCⁱ	CG8355-RD, d. melanogaster CG8355-RE, d. melanogaster

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P24014	Slit, isoform F		DROME		2157	UniRef100_P24014
RT11125p (Fragment)		DROME		1468
IP16087p (Fragment)		DROME		149
P24014-2	RT09975p (Fragment)		DROME		1444	UniRef100_P24014-2

Protein	Similar proteins		Species	Score	Length	Source
P24014	Slit, isoform F		DROME		2157	UniRef90_P24014
LOW QUALITY PROTEIN: protein slit-like		DROOB		2225
LOW QUALITY PROTEIN: protein slit		DROPS		2214
LOW QUALITY PROTEIN: protein slit		DROEL		2181
LOW QUALITY PROTEIN: protein slit		DRORH		2170
+33
P24014-2	RT09975p (Fragment)		DROME		1444	UniRef90_P24014-2
protein slit isoform X1		DROAB		1533
GL16263		DROPE		1426
protein slit isoform X2		DROAB		1522
protein slit isoform X3		DROAB		1509
+27

Protein	Similar proteins		Species	Score	Length	Source
P24014	Slit, isoform F		DROME		2157	UniRef50_P24014
LOW QUALITY PROTEIN: protein slit-like		DROOB		2225
LOW QUALITY PROTEIN: protein slit		DROPS		2214
LOW QUALITY PROTEIN: protein slit		DROEL		2181
LOW QUALITY PROTEIN: protein slit		DRORH		2170
+79
P24014-2	RT09975p (Fragment)		DROME		1444	UniRef50_P24014-2
protein slit isoform X1		DROAB		1533
GL16263		DROPE		1426
Protein slit		BACDO		1596
Protein slit		ZEUCU		1622
+52

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X53959 mRNA Translation: CAA37910.1 AF126540 mRNA Translation: AAD26567.1 AE013599 Genomic DNA Translation: AAF58097.1 AE013599 Genomic DNA Translation: AAF58098.1 AE013599 Genomic DNA Translation: AAM70966.1 AE013599 Genomic DNA Translation: ABV53816.1 AE013599 Genomic DNA Translation: ABV53817.1 M23543 Genomic DNA Translation: AAA72722.1 Different initiation.
PIRⁱ	A36665 B36665
RefSeqⁱ	NP_001097333.1, NM_001103863.3 [P24014-2] NP_001097334.1, NM_001103864.3 [P24014-1] NP_476727.1, NM_057379.4 [P24014-1] NP_476728.1, NM_057380.4 [P24014-2] NP_476729.1, NM_057381.4 [P24014-3]

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1W8A	X-ray	2.80	A	542-733	[»]
SMRⁱ	P24014
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Protein-protein interaction databases

BioGRIDⁱ	62473, 41 interactors
IntActⁱ	P24014, 7 interactors
STRINGⁱ	7227.FBpp0303575

PTM databases

GlyGenⁱ	P24014, 13 sites

GlyGenⁱ

P24014, 13 sites

Proteomic databases

PaxDbⁱ	P24014

PaxDbⁱ

P24014

Genome annotation databases

EnsemblMetazoaⁱ	FBtr0330729; FBpp0303573; FBgn0264089 [P24014-2] FBtr0330730; FBpp0303574; FBgn0264089 [P24014-3] FBtr0330731; FBpp0303575; FBgn0264089 [P24014-1] FBtr0330732; FBpp0303576; FBgn0264089 [P24014-2] FBtr0330733; FBpp0303577; FBgn0264089 [P24014-1]
GeneIDⁱ	36746
KEGGⁱ	dme:Dmel_CG43758
UCSCⁱ	CG8355-RD, d. melanogaster CG8355-RE, d. melanogaster

Organism-specific databases

CTDⁱ	36746
FlyBaseⁱ	FBgn0264089, sli
VEuPathDBⁱ	VectorBase:FBgn0264089

Phylogenomic databases

eggNOGⁱ	KOG4237, Eukaryota
GeneTreeⁱ	ENSGT00940000167217
InParanoidⁱ	P24014
OMAⁱ	ETKCQNN
PhylomeDBⁱ	P24014

Enzyme and pathway databases

Reactomeⁱ	R-DME-376176, Signaling by ROBO receptors R-DME-428890, Role of ABL in ROBO-SLIT signaling
SignaLinkⁱ	P24014

Miscellaneous databases

BioGRID-ORCSⁱ	36746, 0 hits in 3 CRISPR screens
EvolutionaryTraceⁱ	P24014
GenomeRNAiⁱ	36746
Protein Ontology More...PROⁱ	PR:P24014

Gene expression databases

Bgeeⁱ	FBgn0264089, Expressed in central nervous system and 21 other tissues
ExpressionAtlasⁱ	P24014, baseline and differential
Genevisibleⁱ	P24014, DM

Family and domain databases

CDDⁱ	cd00110, LamG, 1 hit
Gene3Dⁱ	3.80.10.10, 5 hits
InterProⁱ	View protein in InterPro IPR013320, ConA-like_dom_sf IPR000483, Cys-rich_flank_reg_C IPR006207, Cys_knot_C IPR001881, EGF-like_Ca-bd_dom IPR013032, EGF-like_CS IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR009030, Growth_fac_rcpt_cys_sf IPR001791, Laminin_G IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR000372, LRRNT
Pfamⁱ	View protein in Pfam PF00008, EGF, 4 hits PF12661, hEGF, 1 hit PF00054, Laminin_G_1, 1 hit PF13855, LRR_8, 6 hits PF01463, LRRCT, 4 hits PF01462, LRRNT, 4 hits
SMARTⁱ	View protein in SMART SM00041, CT, 1 hit SM00181, EGF, 7 hits SM00179, EGF_CA, 6 hits SM00282, LamG, 1 hit SM00369, LRR_TYP, 16 hits SM00082, LRRCT, 4 hits SM00013, LRRNT, 4 hits
SUPFAMⁱ	SSF49899, SSF49899, 1 hit SSF57184, SSF57184, 1 hit
PROSITEⁱ	View protein in PROSITE PS00010, ASX_HYDROXYL, 3 hits PS01185, CTCK_1, 1 hit PS01225, CTCK_2, 1 hit PS00022, EGF_1, 7 hits PS01186, EGF_2, 5 hits PS50026, EGF_3, 7 hits PS01187, EGF_CA, 2 hits PS50025, LAM_G_DOMAIN, 1 hit PS51450, LRR, 20 hits
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SLIT_DROME
Accessionⁱ	P24014Primary (citable) accession number: P24014 Secondary accession number(s): A8DYF5 Q9XYV4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
	Last sequence update:	February 28, 2003
	Last modified:	February 23, 2022
	This is version 221 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Drosophila annotation project

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UniProtKB - P24014 (SLIT_DROME)

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Functionⁱ

GO - Molecular functionⁱ

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Keywords - PTMⁱ

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