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Protein

5-hydroxytryptamine receptor 3A

Gene

Htr3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.1 Publication

Miscellaneous

The HA-stretch region of HTR3A seems to be responsible for the low conductance of HTR3A homomers compared to that of HTR3A/HTR3B heteromers.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 3A
Short name:
5-HT3-A
Short name:
5-HT3A
Alternative name(s):
5-hydroxytryptamine receptor 3
Short name:
5-HT-3
Short name:
5-HT3R
Serotonin receptor 3A
Serotonin-gated ion channel receptor
Gene namesi
Name:Htr3a
Synonyms:5ht3, Htr3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96282 Htr3a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 245ExtracellularSequence analysisAdd BLAST222
Transmembranei246 – 272Helical; Name=1Sequence analysisAdd BLAST27
Topological domaini273 – 277CytoplasmicSequence analysis5
Transmembranei278 – 296Helical; Name=2Sequence analysisAdd BLAST19
Topological domaini297 – 305ExtracellularSequence analysis9
Transmembranei306 – 324Helical; Name=3Sequence analysisAdd BLAST19
Topological domaini325 – 464CytoplasmicSequence analysisAdd BLAST140
Transmembranei465 – 484Helical; Name=4Sequence analysisAdd BLAST20
Topological domaini485 – 487ExtracellularSequence analysis3

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4972
GuidetoPHARMACOLOGYi373

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000000040924 – 4875-hydroxytryptamine receptor 3AAdd BLAST464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi108N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi161 ↔ 175By similarity
Glycosylationi174N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi190N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP23979
PaxDbiP23979
PRIDEiP23979

PTM databases

PhosphoSitePlusiP23979
SwissPalmiP23979

Expressioni

Tissue specificityi

Brain, spinal cord, and heart.

Gene expression databases

CleanExiMM_HTR3A

Interactioni

Subunit structurei

Forms pentahomomeric complex as well as pentaheteromeric complex with HTR3B; homomeric complex is functional but exhibits low conductance with modified voltage dependence, and decreased agonist and antagonist affinity. Interacts with RIC3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-274 5-hydroxytryptamine-3A receptor complex
CPX-275 5-hydroxytryptamine-3A/B receptor complex
DIPiDIP-48769N
STRINGi10090.ENSMUSP00000003826

Chemistry databases

BindingDBiP23979

Structurei

Secondary structure

1487
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP23979
SMRiP23979
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni423 – 459HA-stretchAdd BLAST37

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
HOGENOMiHOG000241519
HOVERGENiHBG106638
InParanoidiP23979
KOiK04819
PhylomeDBiP23979

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR008132 5HT3_rcpt
IPR008133 5HT3_rcpt_A
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR01709 5HT3ARECEPTR
PR01708 5HT3RECEPTOR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.iShow all

Isoform 5-HT3R-A (identifier: P23979-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRLCIPQVLL ALFLSMLTAP GEGSRRRATQ EDTTQPALLR LSDHLLANYK
60 70 80 90 100
KGVRPVRDWR KPTTVSIDVI MYAILNVDEK NQVLTTYIWY RQYWTDEFLQ
110 120 130 140 150
WTPEDFDNVT KLSIPTDSIW VPDILINEFV DVGKSPNIPY VYVHHRGEVQ
160 170 180 190 200
NYKPLQLVTA CSLDIYNFPF DVQNCSLTFT SWLHTIQDIN ITLWRSPEEV
210 220 230 240 250
RSDKSIFINQ GEWELLEVFP QFKEFSIDIS NSYAEMKFYV IIRRRPLFYA
260 270 280 290 300
VSLLLPSIFL MVVDIVGFCL PPDSGERVSF KITLLLGYSV FLIIVSDTLP
310 320 330 340 350
ATIGTPLIGV YFVVCMALLV ISLAETIFIV RLVHKQDLQR PVPDWLRHLV
360 370 380 390 400
LDRIAWILCL GEQPMAHRPP ATFQANKTDD CSGSDLLPAM GNHCSHVGGP
410 420 430 440 450
QDLEKTPRGR GSPLPPPREA SLAVRGLLQE LSSIRHFLEK RDEMREVARD
460 470 480
WLRVGYVLDR LLFRIYLLAV LAYSITLVTL WSIWHYS
Length:487
Mass (Da):56,056
Last modified:March 1, 1992 - v1
Checksum:iD0148867C8536D66
GO
Isoform 5-HT3R-AS (identifier: P23979-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     383-388: Missing.

Show »
Length:481
Mass (Da):55,474
Checksum:i7A9B7B5C38FEC909
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QLC0E9QLC0_MOUSE
5-hydroxytryptamine receptor 3A
Htr3a
489Annotation score:
Q8K1F4Q8K1F4_MOUSE
5-hydroxytryptamine receptor 3A
Htr3a
483Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31E → AR in CAA51089 (PubMed:7683998).Curated1
Sequence conflicti31E → AR in CAA55870 (PubMed:7854052).Curated1
Sequence conflicti31E → AR in CAA55871 (PubMed:7854052).Curated1
Sequence conflicti74I → V in CAA51089 (PubMed:7683998).Curated1
Sequence conflicti302T → TA in CAA51089 (PubMed:7683998).Curated1
Sequence conflicti302T → TA in CAA55870 (PubMed:7854052).Curated1
Sequence conflicti302T → TA in CAA55871 (PubMed:7854052).Curated1
Sequence conflicti384S → SS in CAA80453 (PubMed:8112471).Curated1
Sequence conflicti393H → T in CAA55870 (PubMed:7854052).Curated1
Sequence conflicti393H → T in CAA55871 (PubMed:7854052).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000079383 – 388Missing in isoform 5-HT3R-AS. Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74425 mRNA Translation: AAA37124.1
Z22772, Z22773 Genomic DNA Translation: CAA80453.1
X72395 mRNA Translation: CAA51089.1
X79283 Genomic DNA Translation: CAA55870.1
X79283 Genomic DNA Translation: CAA55871.1
PIRiS41757
RefSeqiNP_038589.2, NM_013561.2
UniGeneiMm.4831

Genome annotation databases

GeneIDi15561
KEGGimmu:15561

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74425 mRNA Translation: AAA37124.1
Z22772, Z22773 Genomic DNA Translation: CAA80453.1
X72395 mRNA Translation: CAA51089.1
X79283 Genomic DNA Translation: CAA55870.1
X79283 Genomic DNA Translation: CAA55871.1
PIRiS41757
RefSeqiNP_038589.2, NM_013561.2
UniGeneiMm.4831

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PIRX-ray3.50A/B/C/D/E32-485[»]
6BE1electron microscopy4.31A/B/C/D/E32-487[»]
ProteinModelPortaliP23979
SMRiP23979
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-274 5-hydroxytryptamine-3A receptor complex
CPX-275 5-hydroxytryptamine-3A/B receptor complex
DIPiDIP-48769N
STRINGi10090.ENSMUSP00000003826

Chemistry databases

BindingDBiP23979
ChEMBLiCHEMBL4972
GuidetoPHARMACOLOGYi373

PTM databases

PhosphoSitePlusiP23979
SwissPalmiP23979

Proteomic databases

MaxQBiP23979
PaxDbiP23979
PRIDEiP23979

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi15561
KEGGimmu:15561

Organism-specific databases

CTDi3359
MGIiMGI:96282 Htr3a

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
HOGENOMiHOG000241519
HOVERGENiHBG106638
InParanoidiP23979
KOiK04819
PhylomeDBiP23979

Miscellaneous databases

PROiPR:P23979
SOURCEiSearch...

Gene expression databases

CleanExiMM_HTR3A

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR008132 5HT3_rcpt
IPR008133 5HT3_rcpt_A
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR01709 5HT3ARECEPTR
PR01708 5HT3RECEPTOR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei5HT3A_MOUSE
AccessioniPrimary (citable) accession number: P23979
Secondary accession number(s): Q61225, Q61226
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 20, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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