Slc6a3

Functionⁱ

Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.4 Publications

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines or cocaine.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	75	Sodium 1; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	77	Sodium 2; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	78	Sodium 1; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	82	Sodium 2By similarity	1
Siteⁱ	105	Contributes to high-affinity binding to cocaineBy similarity	1
Metal bindingⁱ	320	Sodium 2By similarity	1
Metal bindingⁱ	352	Sodium 2By similarity	1
Metal bindingⁱ	417	Sodium 1; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	420	Sodium 1By similarity	1
Metal bindingⁱ	421	Sodium 1By similarity	1

GO - Molecular functionⁱ

dopamine:sodium symporter activity
Source: RGD
Inferred from direct assayⁱ
- 1765147
- 20412389
dopamine binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
drug binding
Source: RGD
Inferred from direct assayⁱ
- 15763134
metal ion binding Source: UniProtKB-KW
neurotransmitter:sodium symporter activity Source: InterPro
protease binding
Source: RGD
Inferred from physical interactionⁱ
- 19419578
protein-containing complex binding
Source: RGD
Inferred from direct assayⁱ
- 15056723
protein N-terminus binding Source: Ensembl
protein phosphatase 2A binding
Source: RGD
Inferred from physical interactionⁱ
- 20035724
signaling receptor binding
Source: RGD
Inferred from physical interactionⁱ
- 18256931

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adenohypophysis development Source: Ensembl
aging
Source: RGD
Inferred from expression patternⁱ
- 14643386
dopamine biosynthetic process Source: Ensembl
dopamine catabolic process Source: Ensembl
dopamine transport
Source: RGD
Inferred from direct assayⁱ
- 1765147
- 20412389
lactation Source: Ensembl
locomotory behavior Source: Ensembl
neurotransmitter uptake Source: Ensembl
positive regulation of multicellular organism growth Source: Ensembl
prepulse inhibition Source: Ensembl
regulation of dopamine metabolic process Source: Ensembl
response to cAMP
Source: RGD
Inferred from direct assayⁱ
- 15234104
response to cocaine Source: Ensembl
response to drug
Source: RGD
Inferred from expression patternⁱ
- 15992364
response to ethanol
Source: RGD
Inferred from direct assayⁱ
- 15897718
response to iron ion
Source: RGD
Inferred from expression patternⁱ
- 12672939
response to nicotine
Source: RGD
Inferred from expression patternⁱ
- 15157990
response to organic cyclic compound
Source: RGD
Inferred from expression patternⁱ
- 12541010
sensory perception of smell Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Neurotransmitter transport, Symport, Transport
Ligand	Metal-binding, Sodium

Enzyme and pathway databases

Reactomeⁱ	R-RNO-379401 Dopamine clearance from the synaptic cleft R-RNO-442660 Na+/Cl- dependent neurotransmitter transporters

Reactomeⁱ

R-RNO-379401 Dopamine clearance from the synaptic cleft
R-RNO-442660 Na+/Cl- dependent neurotransmitter transporters

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Sodium-dependent dopamine transporter Short name: DA transporter Short name: DAT Alternative name(s): Solute carrier family 6 member 3
Gene namesⁱ	Name:Slc6a3
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 1

Organism-specific databases

Rat genome database More...RGDⁱ	3715 Slc6a3

RGDⁱ

3715 Slc6a3

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 68	CytoplasmicSequence analysisAdd BLAST	68
Transmembraneⁱ	69 – 89	Helical; Name=1Sequence analysisAdd BLAST	21
Transmembraneⁱ	96 – 116	Helical; Name=2Sequence analysisAdd BLAST	21
Transmembraneⁱ	140 – 160	Helical; Name=3Sequence analysisAdd BLAST	21
Topological domainⁱ	161 – 236	ExtracellularSequence analysisAdd BLAST	76
Transmembraneⁱ	237 – 255	Helical; Name=4Sequence analysisAdd BLAST	19
Transmembraneⁱ	264 – 281	Helical; Name=5Sequence analysisAdd BLAST	18
Transmembraneⁱ	317 – 334	Helical; Name=6Sequence analysisAdd BLAST	18
Transmembraneⁱ	346 – 367	Helical; Name=7Sequence analysisAdd BLAST	22
Transmembraneⁱ	400 – 419	Helical; Name=8Sequence analysisAdd BLAST	20
Transmembraneⁱ	446 – 464	Helical; Name=9Sequence analysisAdd BLAST	19
Transmembraneⁱ	480 – 500	Helical; Name=10Sequence analysisAdd BLAST	21
Transmembraneⁱ	521 – 540	Helical; Name=11Sequence analysisAdd BLAST	20
Transmembraneⁱ	559 – 577	Helical; Name=12Sequence analysisAdd BLAST	19
Topological domainⁱ	578 – 619	CytoplasmicSequence analysisAdd BLAST	42

Keywords - Cellular componentⁱ

Cell membrane, Membrane

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL338
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	927

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000214754	1 – 619	Sodium-dependent dopamine transporterAdd BLAST		619

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	180 ↔ 189	By similarity
Glycosylationⁱ	181	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	188	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	196	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	204	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	305	InterchainBy similarity

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbⁱ	P23977
PRIDEⁱ	P23977

PTM databases

iPTMnetⁱ	P23977
PhosphoSitePlusⁱ	P23977
SwissPalmⁱ	P23977

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSRNOG00000017302 Expressed in 4 organ(s), highest expression level in brain
Genevisibleⁱ	P23977 RN

Interactionⁱ

Subunit structureⁱ

Homooligomer; disulfide-linked (By similarity). Interacts with PRKCABP and TGFB1I1. Interacts (via N-terminus) with SYNGR3 (via N-terminus). Interacts with SLC18A2. Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location (By similarity).By similarity

GO - Molecular functionⁱ

protease binding
Source: RGD
Inferred from physical interactionⁱ
- 19419578
protein N-terminus binding Source: Ensembl
protein phosphatase 2A binding
Source: RGD
Inferred from physical interactionⁱ
- 20035724
signaling receptor binding
Source: RGD
Inferred from physical interactionⁱ
- 18256931

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	247007, 5 interactors
IntActⁱ	P23977, 2 interactors
Molecular INTeraction database More...MINTⁱ	P23977
STRINGⁱ	10116.ENSRNOP00000047272

Chemistry databases

BindingDBⁱ

P23977

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P23977
SMRⁱ	P23977
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	560 – 589	Interaction with TGFB1I1By similarityAdd BLAST		30

Sequence similaritiesⁱ

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG3659 Eukaryota COG0733 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118857
HOGENOMⁱ	HOG000116406
HOVERGENⁱ	HBG071421
InParanoidⁱ	P23977
KEGG Orthology (KO) More...KOⁱ	K05036
OMAⁱ	PLTFAKM
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G08PX
PhylomeDBⁱ	P23977
TreeFamⁱ	TF343812

Family and domain databases

InterProⁱ	View protein in InterPro IPR000175 Na/ntran_symport IPR002436 Na/ntran_symport_dopamine IPR037272 SNS_sf
PANTHERⁱ	PTHR11616 PTHR11616, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00209 SNF, 1 hit
PRINTSⁱ	PR01202 DOPTRANSPORT PR00176 NANEUSMPORT
SUPFAMⁱ	SSF161070 SSF161070, 1 hit
PROSITEⁱ	View protein in PROSITE PS00610 NA_NEUROTRAN_SYMP_1, 1 hit PS00754 NA_NEUROTRAN_SYMP_2, 1 hit PS50267 NA_NEUROTRAN_SYMP_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P23977-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSKSKCSVGP MSSVVAPAKE SNAVGPREVE LILVKEQNGV QLTNSTLINP 
        60         70         80         90        100
PQTPVEAQER ETWSKKIDFL LSVIGFAVDL ANVWRFPYLC YKNGGGAFLV 
       110        120        130        140        150
PYLLFMVIAG MPLFYMELAL GQFNREGAAG VWKICPVLKG VGFTVILISF 
       160        170        180        190        200
YVGFFYNVII AWALHYFFSS FTMDLPWIHC NNTWNSPNCS DAHASNSSDG 
       210        220        230        240        250
LGLNDTFGTT PAAEYFERGV LHLHQSRGID DLGPPRWQLT ACLVLVIVLL 
       260        270        280        290        300
YFSLWKGVKT SGKVVWITAT MPYVVLTALL LRGVTLPGAM DGIRAYLSVD 
       310        320        330        340        350
FYRLCEASVW IDAATQVCFS LGVGFGVLIA FSSYNKFTNN CYRDAIITTS 
       360        370        380        390        400
INSLTSFSSG FVVFSFLGYM AQKHNVPIRD VATDGPGLIF IIYPEAIATL 
       410        420        430        440        450
PLSSAWAAVF FLMLLTLGID SAMGGMESVI TGLVDEFQLL HRHRELFTLG 
       460        470        480        490        500
IVLATFLLSL FCVTNGGIYV FTLLDHFAAG TSILFGVLIE AIGVAWFYGV 
       510        520        530        540        550
QQFSDDIKQM TGQRPNLYWR LCWKLVSPCF LLYVVVVSIV TFRPPHYGAY 
       560        570        580        590        600
IFPDWANALG WIIATSSMAM VPIYATYKFC SLPGSFREKL AYAITPEKDH 
       610 
QLVDRGEVRQ FTLRHWLLL

Length:619

Mass (Da):68,746

Last modified:March 1, 1992 - v1

Checksum:ⁱDF3A30C981095D24

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	597	E → K in AAB21099 (PubMed:1765147).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M80233 mRNA Translation: AAA41100.1 M80570 mRNA Translation: AAA73143.1 S76145 mRNA Translation: AAB21099.1
PIRⁱ	I59558 S20346
NCBI Reference Sequences More...RefSeqⁱ	NP_036826.1, NM_012694.2
UniGeneⁱ	Rn.10093

Genome annotation databases

Ensemblⁱ	ENSRNOT00000040291; ENSRNOP00000047272; ENSRNOG00000017302 ENSRNOT00000079987; ENSRNOP00000070728; ENSRNOG00000017302
GeneIDⁱ	24898
KEGGⁱ	rno:24898
UCSC genome browser More...UCSCⁱ	RGD:3715 rat

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P23977	Sodium-dependent dopamine transporter		HUMAN		620	UniRef90_Q01959
Sodium-dependent dopamine transporter		MACFA		620
Sodium-dependent dopamine transporter		MOUSE		619
Transporter		FUKDA		766
Transporter		MACMU		620
+41

Protein	Similar proteins		Species	Score	Length	Source
P23977	Sodium-dependent dopamine transporter		HUMAN		620	UniRef50_Q01959
Sodium-dependent dopamine transporter		MACFA		620
Sodium-dependent dopamine transporter		MOUSE		619
Sodium-dependent noradrenaline transporter		BOVIN		615
Transporter		FUKDA		766
+241

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M80233 mRNA Translation: AAA41100.1 M80570 mRNA Translation: AAA73143.1 S76145 mRNA Translation: AAB21099.1
PIRⁱ	I59558 S20346
RefSeqⁱ	NP_036826.1, NM_012694.2
UniGeneⁱ	Rn.10093

3D structure databases

ProteinModelPortalⁱ	P23977
SMRⁱ	P23977
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	247007, 5 interactors
IntActⁱ	P23977, 2 interactors
MINTⁱ	P23977
STRINGⁱ	10116.ENSRNOP00000047272

Chemistry databases

BindingDBⁱ	P23977
ChEMBLⁱ	CHEMBL338
GuidetoPHARMACOLOGYⁱ	927

PTM databases

iPTMnetⁱ	P23977
PhosphoSitePlusⁱ	P23977
SwissPalmⁱ	P23977

Proteomic databases

PaxDbⁱ	P23977
PRIDEⁱ	P23977

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000040291; ENSRNOP00000047272; ENSRNOG00000017302 ENSRNOT00000079987; ENSRNOP00000070728; ENSRNOG00000017302
GeneIDⁱ	24898
KEGGⁱ	rno:24898
UCSCⁱ	RGD:3715 rat

Organism-specific databases

CTDⁱ	6531
RGDⁱ	3715 Slc6a3

Phylogenomic databases

eggNOGⁱ	KOG3659 Eukaryota COG0733 LUCA
GeneTreeⁱ	ENSGT00760000118857
HOGENOMⁱ	HOG000116406
HOVERGENⁱ	HBG071421
InParanoidⁱ	P23977
KOⁱ	K05036
OMAⁱ	PLTFAKM
OrthoDBⁱ	EOG091G08PX
PhylomeDBⁱ	P23977
TreeFamⁱ	TF343812

Enzyme and pathway databases

Reactomeⁱ	R-RNO-379401 Dopamine clearance from the synaptic cleft R-RNO-442660 Na+/Cl- dependent neurotransmitter transporters

Reactomeⁱ

R-RNO-379401 Dopamine clearance from the synaptic cleft
R-RNO-442660 Na+/Cl- dependent neurotransmitter transporters

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P23977

PROⁱ

PR:P23977

Gene expression databases

Bgeeⁱ	ENSRNOG00000017302 Expressed in 4 organ(s), highest expression level in brain
Genevisibleⁱ	P23977 RN

Family and domain databases

InterProⁱ	View protein in InterPro IPR000175 Na/ntran_symport IPR002436 Na/ntran_symport_dopamine IPR037272 SNS_sf
PANTHERⁱ	PTHR11616 PTHR11616, 1 hit
Pfamⁱ	View protein in Pfam PF00209 SNF, 1 hit
PRINTSⁱ	PR01202 DOPTRANSPORT PR00176 NANEUSMPORT
SUPFAMⁱ	SSF161070 SSF161070, 1 hit
PROSITEⁱ	View protein in PROSITE PS00610 NA_NEUROTRAN_SYMP_1, 1 hit PS00754 NA_NEUROTRAN_SYMP_2, 1 hit PS50267 NA_NEUROTRAN_SYMP_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SC6A3_RAT
Accessionⁱ	P23977Primary (citable) accession number: P23977
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
	Last sequence update:	March 1, 1992
	Last modified:	November 7, 2018
	This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P23977 (SC6A3_RAT)

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Sodium-dependent dopamine transporter

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ