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Protein

Chymase

Gene

CMA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

Catalytic activityi

Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei66Charge relay system1
Active sitei110Charge relay system1
Active sitei203Charge relay system1

GO - Molecular functioni

GO - Biological processi

  • angiotensin maturation Source: Reactome
  • basement membrane disassembly Source: BHF-UCL
  • cellular response to glucose stimulus Source: Ensembl
  • extracellular matrix disassembly Source: BHF-UCL
  • interleukin-1 beta biosynthetic process Source: BHF-UCL
  • midbrain development Source: Ensembl
  • positive regulation of angiogenesis Source: Ensembl
  • regulation of inflammatory response Source: BHF-UCL

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.39 2681
ReactomeiR-HSA-1433557 Signaling by SCF-KIT
R-HSA-1592389 Activation of Matrix Metalloproteinases
R-HSA-2022377 Metabolism of Angiotensinogen to Angiotensins
SABIO-RKiP23946

Protein family/group databases

MEROPSiS01.140

Names & Taxonomyi

Protein namesi
Recommended name:
Chymase (EC:3.4.21.39)
Alternative name(s):
Alpha-chymase
Mast cell protease I
Gene namesi
Name:CMA1
Synonyms:CYH, CYM
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000092009.10
HGNCiHGNC:2097 CMA1
MIMi118938 gene
neXtProtiNX_P23946

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi1215
OpenTargetsiENSG00000092009
PharmGKBiPA26623

Chemistry databases

ChEMBLiCHEMBL4068
DrugBankiDB03814 2-(N-Morpholino)-Ethanesulfonic Acid
DB04016 2-[3-({Methyl[1-(2-Naphthoyl)Piperidin-4-Yl]Amino}Carbonyl)-2-Naphthyl]-1-(1-Naphthyl)-2-Oxoethylphosphonic Acid
DB07680 [(1S)-1-(5-CHLORO-1-BENZOTHIEN-3-YL)-2-(2-NAPHTHYLAMINO)-2-OXOETHYL]PHOSPHONIC ACID
DB03297 Benzylsulfinic Acid
DB03149 Phenylalanylmethane
GuidetoPHARMACOLOGYi2340

Polymorphism and mutation databases

BioMutaiCMA1
DMDMi126825

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Add BLAST19
PropeptideiPRO_000002743320 – 21Activation peptide1 Publication2
ChainiPRO_000002743422 – 247ChymaseAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 67
Glycosylationi80N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi103N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi144 ↔ 209
Disulfide bondi175 ↔ 188

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP23946
PeptideAtlasiP23946
PRIDEiP23946
ProteomicsDBi54171

PTM databases

iPTMnetiP23946
PhosphoSitePlusiP23946

Miscellaneous databases

PMAP-CutDBiP23946

Expressioni

Tissue specificityi

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.1 Publication

Gene expression databases

BgeeiENSG00000092009
CleanExiHS_CMA1
GenevisibleiP23946 HS

Organism-specific databases

HPAiCAB000363
HPA052634

Interactioni

Protein-protein interaction databases

BioGridi107624, 20 interactors
STRINGi9606.ENSP00000250378

Chemistry databases

BindingDBiP23946

Structurei

Secondary structure

1247
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 42Combined sources7
Beta strandi44 – 47Combined sources4
Beta strandi49 – 57Combined sources9
Beta strandi60 – 63Combined sources4
Helixi65 – 67Combined sources3
Beta strandi70 – 77Combined sources8
Beta strandi79 – 83Combined sources5
Beta strandi89 – 98Combined sources10
Turni104 – 106Combined sources3
Beta strandi112 – 118Combined sources7
Beta strandi124 – 126Combined sources3
Beta strandi143 – 153Combined sources11
Beta strandi163 – 170Combined sources8
Helixi172 – 175Combined sources4
Turni183 – 185Combined sources3
Beta strandi186 – 190Combined sources5
Turni192 – 194Combined sources3
Beta strandi206 – 209Combined sources4
Beta strandi212 – 219Combined sources8
Beta strandi228 – 232Combined sources5
Helixi233 – 246Combined sources14

3D structure databases

ProteinModelPortaliP23946
SMRiP23946
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23946

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 245Peptidase S1PROSITE-ProRule annotationAdd BLAST224

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118895
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP23946
KOiK01329
OMAiHYRPWIN
OrthoDBiEOG091G0G5F
PhylomeDBiP23946
TreeFamiTF333630

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P23946-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLPLPLLL FLLCSRAEAG EIIGGTECKP HSRPYMAYLE IVTSNGPSKF
60 70 80 90 100
CGGFLIRRNF VLTAAHCAGR SITVTLGAHN ITEEEDTWQK LEVIKQFRHP
110 120 130 140 150
KYNTSTLHHD IMLLKLKEKA SLTLAVGTLP FPSQFNFVPP GRMCRVAGWG
160 170 180 190 200
RTGVLKPGSD TLQEVKLRLM DPQACSHFRD FDHNLQLCVG NPRKTKSAFK
210 220 230 240
GDSGGPLLCA GVAQGIVSYG RSDAKPPAVF TRISHYRPWI NQILQAN
Length:247
Mass (Da):27,325
Last modified:March 1, 1992 - v1
Checksum:iDC1464A049ED6B00
GO
Isoform 2 (identifier: P23946-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: Missing.

Note: No experimental confirmation available.
Show »
Length:136
Mass (Da):14,893
Checksum:iDA8A15F89D222FC4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28C → S in AAB26828 (PubMed:8495723).Curated1
Sequence conflicti131 – 132FP → AV AA sequence (PubMed:8144971).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01177046G → R. Corresponds to variant dbSNP:rs5246Ensembl.1
Natural variantiVAR_01177166H → R1 PublicationCorresponds to variant dbSNP:rs5247Ensembl.1
Natural variantiVAR_02919098R → H. Corresponds to variant dbSNP:rs13306252Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0569471 – 111Missing in isoform 2. 1 PublicationAdd BLAST111

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64269 Genomic DNA Translation: AAA52020.1
M69137 Genomic DNA Translation: AAA52021.1
M69136 mRNA Translation: AAA52019.1
AB451464 mRNA Translation: BAG70278.1
AL132800 Genomic DNA No translation available.
CH471078 Genomic DNA Translation: EAW66007.1
BC069110 mRNA Translation: AAH69110.1
BC069370 mRNA Translation: AAH69370.1
BC069490 mRNA Translation: AAH69490.1
BC103974 mRNA Translation: AAI03975.1
BC103975 mRNA Translation: AAI03976.1
S61334 mRNA Translation: AAB26828.1
X59072 Genomic DNA Translation: CAA41796.1
CCDSiCCDS76666.1 [P23946-2]
CCDS9630.1 [P23946-1]
PIRiA40967 KYHUCM
RefSeqiNP_001295012.1, NM_001308083.1 [P23946-2]
NP_001827.1, NM_001836.4 [P23946-1]
UniGeneiHs.135626

Genome annotation databases

EnsembliENST00000206446; ENSP00000206446; ENSG00000092009 [P23946-2]
ENST00000250378; ENSP00000250378; ENSG00000092009 [P23946-1]
GeneIDi1215
KEGGihsa:1215
UCSCiuc001wpp.2 human [P23946-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCMA1_HUMAN
AccessioniPrimary (citable) accession number: P23946
Secondary accession number(s): B5BUM8
, Q16018, Q3SY36, Q3SY37, Q9UDH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 20, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families
  8. UniProtKB entry view manual
    User manual for the UniProtKB entry view

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