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UniProtKB - P23917 (MAK_ECOLI)
Protein
Fructokinase
Gene
mak
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the phosphorylation of fructose to fructose-6-P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine.
2 PublicationsCatalytic activityi
- EC:2.7.1.4
Kineticsi
Catalytic efficiency with D-fructose as substrate is 55-fold higher than that with D-glucose.
- KM=1.3 mM for D-fructose (at 25 degrees Celsius and pH 7.6)1 Publication
- KM=1.8 mM for ATP (at 25 degrees Celsius and pH 7.6)1 Publication
- KM=59 mM for glucose (at 25 degrees Celsius and pH 7.6)1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 4 – 11 | ATPSequence analysis | 8 | |
Nucleotide bindingi | 133 – 140 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- fructokinase activity Source: EcoCyc
- hexokinase activity Source: GO_Central
- mannokinase activity Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Carbohydrate metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11288-MONOMER |
SABIO-RKi | P23917 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mak Synonyms:yajF Ordered Locus Names:b0394, JW0385 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000095689 | 1 – 302 | FructokinaseAdd BLAST | 302 |
Proteomic databases
jPOSTi | P23917 |
PaxDbi | P23917 |
PRIDEi | P23917 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259818, 15 interactors |
DIPi | DIP-11288N |
IntActi | P23917, 30 interactors |
STRINGi | 511145.b0394 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ROK (NagC/XylR) family.Curated
Phylogenomic databases
eggNOGi | COG1940, Bacteria |
HOGENOMi | CLU_036604_0_3_6 |
InParanoidi | P23917 |
OMAi | DHLVMIT |
PhylomeDBi | P23917 |
Family and domain databases
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR000600, ROK |
PANTHERi | PTHR18964, PTHR18964, 1 hit |
Pfami | View protein in Pfam PF00480, ROK, 1 hit |
SUPFAMi | SSF53067, SSF53067, 1 hit |
PROSITEi | View protein in PROSITE PS01125, ROK, 1 hit |
i Sequence
Sequence statusi: Complete.
P23917-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRIGIDLGGT KTEVIALGDA GEQLYRHRLP TPRDDYRQTI ETIATLVDMA
60 70 80 90 100
EQATGQRGTV GMGIPGSISP YTGVVKNANS TWLNGQPFDK DLSARLQREV
110 120 130 140 150
RLANDANCLA VSEAVDGAAA GAQTVFAVII GTGCGAGVAF NGRAHIGGNG
160 170 180 190 200
TAGEWGHNPL PWMDEDELRY REEVPCYCGK QGCIETFISG TGFAMDYRRL
210 220 230 240 250
SGHALKGSEI IRLVEESDPV AELALRRYEL RLAKSLAHVV NILDPDVIVL
260 270 280 290 300
GGGMSNVDRL YQTVGQLIKQ FVFGGECETP VRKAKHGDSS GVRGAAWLWP
QE
Sequence cautioni
The sequence AAB18118 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76979 Genomic DNA Translation: CAA54284.1 U73857 Genomic DNA Translation: AAB18118.1 Different initiation. U00096 Genomic DNA Translation: AAC73497.2 AP009048 Genomic DNA Translation: BAE76175.1 M64787 Genomic DNA Translation: AAA23475.1 |
PIRi | B64768 |
RefSeqi | NP_414928.2, NC_000913.3 WP_001219309.1, NZ_SSZK01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC73497; AAC73497; b0394 BAE76175; BAE76175; BAE76175 |
GeneIDi | 949086 |
KEGGi | ecj:JW0385 eco:b0394 |
PATRICi | fig|1411691.4.peg.1884 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76979 Genomic DNA Translation: CAA54284.1 U73857 Genomic DNA Translation: AAB18118.1 Different initiation. U00096 Genomic DNA Translation: AAC73497.2 AP009048 Genomic DNA Translation: BAE76175.1 M64787 Genomic DNA Translation: AAA23475.1 |
PIRi | B64768 |
RefSeqi | NP_414928.2, NC_000913.3 WP_001219309.1, NZ_SSZK01000009.1 |
3D structure databases
AlphaFoldDBi | P23917 |
SMRi | P23917 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259818, 15 interactors |
DIPi | DIP-11288N |
IntActi | P23917, 30 interactors |
STRINGi | 511145.b0394 |
Proteomic databases
jPOSTi | P23917 |
PaxDbi | P23917 |
PRIDEi | P23917 |
Genome annotation databases
EnsemblBacteriai | AAC73497; AAC73497; b0394 BAE76175; BAE76175; BAE76175 |
GeneIDi | 949086 |
KEGGi | ecj:JW0385 eco:b0394 |
PATRICi | fig|1411691.4.peg.1884 |
Organism-specific databases
EchoBASEi | EB1265 |
Phylogenomic databases
eggNOGi | COG1940, Bacteria |
HOGENOMi | CLU_036604_0_3_6 |
InParanoidi | P23917 |
OMAi | DHLVMIT |
PhylomeDBi | P23917 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11288-MONOMER |
SABIO-RKi | P23917 |
Miscellaneous databases
PROi | PR:P23917 |
Family and domain databases
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR000600, ROK |
PANTHERi | PTHR18964, PTHR18964, 1 hit |
Pfami | View protein in Pfam PF00480, ROK, 1 hit |
SUPFAMi | SSF53067, SSF53067, 1 hit |
PROSITEi | View protein in PROSITE PS01125, ROK, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAK_ECOLI | |
Accessioni | P23917Primary (citable) accession number: P23917 Secondary accession number(s): P71316, P75705, Q2MC31 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 1992 |
Last sequence update: | June 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families